Adult LacZ Expression Data for Ccdc57 (MALA)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Ccdc57 Knockout First EPD0037_3_D11 Ccdc57 Knockout First EPD0037_3_D11 Ccdc57 Knockout First EPD0037_3_D11
Genotype Ccdc57/+ Ccdc57/+ Ccdc57/+
Gender Male Female Male
Age In Weeks 24.1 6.4 13.3
Adrenal Gland absent absent present
Bone absent absent absent
Brain present present present
Brown Adipose Tissue absent absent absent
Cartilage present present present
Colon absent absent absent
Eye absent absent absent
Gall Bladder No data No data absent
Heart absent absent absent
Kidney absent absent present
Large Intestine absent absent absent
Liver absent absent absent
Lung present present present
Mammary Gland absent absent absent
Mesenteric Lymph Node absent absent absent
Nasal Epithelia No data No data No data
Oesophagus absent absent No data
Oral Epithelia absent absent absent
Ovaries No data present No data
Oviduct No data present No data
Pancreas absent absent ambiguous
Parathyroid present present No data
Peripheral Nervous System present ambiguous present
Peyers Patch absent absent absent
Pituitary Gland No data No data present
Prostate absent No data absent
Skeletal Muscle present present present
Skin absent absent absent
Small Intestine absent absent absent
Spinal Cord present present present
Spleen absent absent ambiguous
Stomach absent absent absent
Testis present No data present
Thymus absent absent absent
Thyroid absent absent No data
Trachea present present No data
Urinary System absent absent absent
Uterus No data absent No data
Vas Deferens absent No data absent
Vascular System absent absent absent
White Adipose Tissue absent absent absent
Images
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Lung
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype