Auditory Brainstem Response (ABR) measurement for Apoetm1UncMGP Pipeline 2

Results Summary

  • ABR thresholds normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean Threshold is plotted (+/- population standard deviation). The green shaded area indicates the mean threshold (+/- 1SD and +/- 2SD) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and are plotted as a function of absolute sound pressure level (SPL) and SPL relative to threshold. Means are plotted (+/- population standard deviation).

ABR Thresholds Summary

ABR Thresholds for Apoe<tm1Unc> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
MAKL13.1g M00020297 ApoE/+(locked) Female 13.1 31 19 16 28 31
MAKL27.1h M00020489 ApoE/+(locked) Female 12.8 34 22 31 34 46
MAKL26.1a M00020431 ApoE/+(locked) Male 13.2 31 16 25 19 46
MAKL27.1f M00020487 ApoE/+(locked) Male 12.8 28 19 25 25 49
MAKL33.1b M00020509 ApoE/+(locked) Male 12.8 37 19 25 31 43
ApoE/+ Average 12.94 32.2 19 24.4 27.4 43
ApoE/+ Standard Deviation 0.17 3.06 1.9 4.8 5.16 6.29
ApoE/+ Standard Error 0.08 1.37 .85 2.15 2.31 2.81
MAKL18.1f M00020322 ApoE/ApoE(locked) Female 13.1 34 16 19 25 46
MAKL36.1d M00020592 ApoE/ApoE(locked) Female 12.3 28 13 19 19 34
MAKL38.1f M00020599 ApoE/ApoE(locked) Female 12.6 46 16 16 28 31
MAKL41.1h M00020616 ApoE/ApoE(locked) Female 12.6 28 13 13 19 31
MAKL18.1b M00020318 ApoE/ApoE(locked) Male 13.1 31 16 22 19 43
MAKL18.1c M00020319 ApoE/ApoE(locked) Male 13.1 34 22 28 31 40
MAKL35.3c M00047200 ApoE/ApoE(locked) Male 12.8 49 25 37 31 37
MAKL55.2a M00047269 ApoE/ApoE(locked) Male 12.9 49 19 43 34 43
ApoE/ApoE Average 12.81 37.38 17.5 24.63 25.75 38.13
ApoE/ApoE Standard Deviation 0.28 8.54 3.97 9.89 5.76 5.4
ApoE/ApoE Standard Error 0.1 3.02 1.4 3.5 2.04 1.91
Controls
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
MAKL13.1c M00020294 +/+(locked) Female 12.9 37 22 25 22 34
MAKL13.1d M00020295 +/+(locked) Female 12.9 28 22 25 31 46
MAKL13.1h M00020298 +/+(locked) Female 12.9 28 13 16 16 37
MAKL19.1d M00020345 +/+(locked) Female 13.8 34 16 16 22 43
MAKL19.1f M00020347 +/+(locked) Female 13.8 31 13 16 25 37
MAKL20.1d M00020369 +/+(locked) Female 13.2 34 16 25 16 31
MAKL26.1c M00020433 +/+(locked) Male 13.2 34 13 16 19 40
MAKL26.1e M00020435 +/+(locked) Male 13.2 43 19 31 34 58
MAKL26.1g M00020437 +/+(locked) Male 13.2 37 19 25 28 37
MAKL3.3a M00047181 +/+(locked) Male 12.9 34 19 22 22 31
MAKL3.3c M00047183 +/+(locked) Male 12.9 40 19 34 28 40
MAKL30.1a M00020528 +/+(locked) Male 12.3 22 13 22 19 46
MAKL33.1d M00020511 +/+(locked) Male 12.8 34 16 19 25 40
+/+ Average 13.08 33.54 16.92 22.46 23.62 40
+/+ Standard Deviation 0.39 5.24 3.2 5.62 5.34 6.96
+/+ Standard Error 0.11 1.45 .89 1.56 1.48 1.93