Auditory Brainstem Response (ABR) measurement for Kctd10Gt(RRG305)Byg MGP Pipeline 2 (Kctd10 Generic Baseline)

Results Summary

  • ABR thresholds normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean Threshold is plotted (+/- population standard deviation). The green shaded area indicates the mean threshold (+/- 1SD and +/- 2SD) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and are plotted as a function of absolute sound pressure level (SPL) and SPL relative to threshold. Means are plotted (+/- population standard deviation).

ABR Thresholds Summary

ABR Thresholds for Kctd10<Gt(RRG305)Byg> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
MAKJ117.1g M00020937 Kctd10/+(locked) Female 12.9 49 25 28 34 46
MAKJ122.1g M00020979 Kctd10/+(locked) Female 13.1 40 19 31 34 40
MAKJ130.1f M00020993 Kctd10/+(locked) Female 12.9 40 19 13 19 31
MAKJ130.1h M00020995 Kctd10/+(locked) Female 13.0 43 19 16 31 40
MAKJ130.1i M00020996 Kctd10/+(locked) Female 12.9 40 22 22 25 40
MAKJ109.1d M00020963 Kctd10/+(locked) Male 12.8 61 22 28 19 34
MAKJ117.1c M00020933 Kctd10/+(locked) Male 12.9 43 28 25 31 40
MAKJ117.1d M00020934 Kctd10/+(locked) Male 12.9 52 19 28 37 37
MAKJ117.1e M00020935 Kctd10/+(locked) Male 12.9 49 25 22 31 40
MAKJ123.1a M00020980 Kctd10/+(locked) Male 12.9 61 13 19 25 46
Kctd10/+ Average 12.92 47.8 21.1 23.2 28.6 39.4
Kctd10/+ Standard Deviation 0.07 7.73 4.04 5.56 5.97 4.41
Kctd10/+ Standard Error 0.02 2.44 1.28 1.76 1.89 1.39
Controls
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
MAKJ117.1f M00020936 +/+(locked) Female 12.9 55 31 25 25 40
MAKJ122.1e M00020977 +/+(locked) Female 13.1 52 25 34 37 49
MAKJ130.1g M00020994 +/+(locked) Female 12.9 28 16 13 22 28
MAKJ130.1j M00020997 +/+(locked) Female 13.0 46 31 34 25 34
MAKJ109.1a M00020960 +/+(locked) Male 12.8 52 13 16 16 31
MAKJ109.1c M00020962 +/+(locked) Male 12.8 37 19 13 19 34
MAKJ111.1a M00020921 +/+(locked) Male 12.5 49 10 16 19 40
MAKJ111.1b M00020922 +/+(locked) Male 12.5 46 19 34 28 37
MAKJ111.1c M00020923 +/+(locked) Male 12.5 40 16 16 13 31
+/+ Average 12.78 45 20 22.33 22.67 36
+/+ Standard Deviation 0.21 8.12 7.07 8.88 6.75 6
+/+ Standard Error 0.07 2.71 2.36 2.96 2.25 2