Phenotyping Data for Mysm1 (MAHN) Associated with 'hematopoietic system' (MP:0005397)

Haematology (CBC): Haematology (Avertin) - significant parameters
Haematology (week 14) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted mice are terminally anaesthetised with avertin, and blood is collected into EDTA coated tubes via the retro-orbital sinus. Whole blood is analysed for the following parameters: White and red blood cell counts, mean corpuscular volume, haemoglobin, erythrocyte indices (haematocrit, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, red blood cell distribution width), platelet counts and mean platelet volume. All blood samples are analysed with an automatic haematology analyser using volume impedance principle (scilVet Animal Blood Counter, RAB 015 A Ind.E, 22.02.01).
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
White Blood Cell Count view graph MP:0000221 - decreased leukocyte cell number
Red Blood Cell Count view graph MP:0002875 - decreased erythrocyte cell number
Mean Corpuscular Volume view graph MP:0002590 - increased mean corpuscular volume
Haemoglobin view graph MP:0002874 - decreased hemoglobin content
Haematocrit view graph MP:0000208 - decreased hematocrit
Mean Corpuscular Haemoglobin view graph MP:0005561 - increased mean corpuscular hemoglobin
Mean Corpuscular Haemoglobin Conc. view graph MP:0005641 - increased mean corpuscular hemoglobin concentration
Red Blood Cell Distribution Width view graph MP:0010067 - increased red blood cell distribution width
Platelet Count view graph MP:0005505 - increased platelet cell number
Mean Platelet Volume view graph MP:0002599 - increased mean platelet volume
Peripheral Blood Leukocytes: PBL FACS Analysis - FC500 (4 panel) - significant parameters
Fluorescence Activated Cell Sorting (week 14) [Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning]: FACS analysis is performed on heparinised blood for the following parameters: Percentages of CD4+ and CD8+ T cells, NKT cells, NK cells, B cells, Granulocytes and Monocytes are presented relative to the total CD45+ WBC population. Percentages of CD4+CD25+ Treg cells are presented relative to the total CD4+ T cell population. Percentages of mature IgD+ B cells are presented relative to the total B cell population. All samples are analysed on a Beckman-Coulter FC-500.
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
T cell CD4+ percentage view graph MP:0008074 - increased CD4-positive T cell number
Treg cell CD25+ percentage
T cell CD8+ percentage
NKT cell percentage
NK cell percentage
B cell CD19+ percentage view graph MP:0005017 - decreased B cell number
Mature B cell IgD+ percentage view graph MP:0008211 - decreased mature B cell number
Granulocyte Gr1+ percentage view graph MP:0000322 - increased granulocyte number
Monocyte percentage