Auditory Brainstem Response (ABR) measurement for Mysm1tm1a(KOMP)WtsiMGP Pipeline 2

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal
  • (Homozygote n=3)

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Mysm1<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAHN48.1c M00077901 Mysm1/+(locked) Female 12.5 25 52 31 28 46 49
MAHN48.1d M00077902 Mysm1/+(locked) Female 12.5 25 70 37 37 37 43
MAHN48.1f M00077904 Mysm1/+(locked) Female 12.5 25 40 22 25 31 49
MAHN49.1d M00077909 Mysm1/+(locked) Male 12.6 25 34 28 28 52 85
MAHN55.1d M00078009 Mysm1/+(locked) Male 12.6 19 55 37 40 49 67
MAHN55.1e M00078010 Mysm1/+(locked) Male 12.6 31 55 28 37 64 58
Mysm1/+ Average 12.55 25 51 30.5 32.5 46.5 58.5
Mysm1/+ Standard Deviation 0.05 3.46 11.58 5.32 5.68 10.59 14.12
Mysm1/+ Standard Error 0.02 1.41 4.73 2.17 2.32 4.33 5.76
MAHN35.2a M00097368 Mysm1/Mysm1(locked) Male 12.6 28 40 31 25 28 43
MAHN49.2a M00097443 Mysm1/Mysm1(locked) Male 13.2 28 49 22 34 34 40
MAHN49.2b M00097444 Mysm1/Mysm1(locked) Male 13.2 28 70 43 37 37 49
Mysm1/Mysm1 Average 13.0 28 53 32 32 33 44
Mysm1/Mysm1 Standard Deviation 0.28 0 12.57 8.6 5.1 3.74 3.74
Mysm1/Mysm1 Standard Error 0.16 0 7.26 4.97 2.94 2.16 2.16
MAHN57.1d M00077949 Mysm1:failed(locked) Female 12.9 25 49 34 22 37 37
MAHN55.1g M00078012 Mysm1:failed(locked) Male 12.6 22 58 31 34 37 64
Mysm1:failed Average 12.75 23.5 53.5 32.5 28 37 50.5
Mysm1:failed Standard Deviation 0.15 1.5 4.5 1.5 6 0 13.5
Mysm1:failed Standard Error 0.11 1.06 3.18 1.06 4.24 0 9.55
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAHN38.1f M00077862 +/+(locked) Female 12.5 25 34 22 19 31 46
MAHN57.1e M00077950 +/+(locked) Female 12.9 25 34 28 22 31 43
MAHN55.1c M00078008 +/+(locked) Male 12.6 19 40 19 31 37 40
MAHN55.1f M00078011 +/+(locked) Male 12.6 25 73 40 37 37 43
+/+ Average 12.65 23.5 45.25 27.25 27.25 34 43
+/+ Standard Deviation 0.15 2.6 16.21 8.04 7.15 3 2.12
+/+ Standard Error 0.08 1.3 8.1 4.02 3.58 1.5 1.06