Adult LacZ Expression Data for Arid2 (MAEV)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Arid2 Knockout First B05 - EPD0027_2 Arid2 Knockout First B05 - EPD0027_2 Arid2 Knockout First B05 - EPD0027_2 Arid2 Knockout First B05 - EPD0027_2
Genotype Arid2/+ Arid2/+ Arid2/+ Arid2/+
Gender Female Male Male Female
Age In Weeks 13.5 7.2 10.6 10.6
Adrenal Gland absent No data present present
Bone absent absent absent absent
Brain present present present present
Brown Adipose Tissue absent ambiguous ambiguous absent
Cartilage ambiguous present present present
Colon absent present present present
Eye absent absent absent absent
Gall Bladder No data present absent absent
Heart absent present present present
Kidney ambiguous present present present
Large Intestine absent present present present
Liver absent absent present absent
Lung ambiguous present present present
Mammary Gland absent absent absent absent
Mesenteric Lymph Node absent present present ambiguous
Nasal Epithelia No data No data No data No data
Oesophagus absent present present present
Oral Epithelia absent ambiguous absent absent
Ovaries present No data No data present
Oviduct absent No data No data present
Pancreas absent present present present
Parathyroid present present present present
Peripheral Nervous System present present present present
Peyers Patch absent present present present
Pituitary Gland No data present present present
Prostate No data present present No data
Skeletal Muscle ambiguous present present present
Skin present present present present
Small Intestine absent present present present
Spinal Cord present present present present
Spleen absent present present present
Stomach ambiguous present present present
Testis No data present present No data
Thymus absent present present present
Thyroid absent present present present
Trachea absent present present present
Urinary System ambiguous present present present
Uterus absent No data No data present
Vas Deferens No data present present No data
Vascular System absent ambiguous absent absent
White Adipose Tissue absent absent absent absent
Images
  • Tissue: Adrenal gland
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Hind limb
    Description: -
    Genotype: Heterozygous
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Lung
    Description: -
    Genotype: Heterozygous
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Heterozygous
  • Tissue: Pinna
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: -
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype