Auditory Brainstem Response (ABR) measurement for Baz1btm2a(KOMP)WtsiMGP Pipeline 2

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Baz1b<tm2a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MACW41.1b M00078072 Baz1b/+(locked) Female 12.7 31 76 37 37 40 40
MACW59.1c M00078108 Baz1b/+(locked) Female 12.9 19 49 25 28 25 40
MACW58.1a M00078110 Baz1b/+(locked) Male 12.6 28 52 22 25 46 43
MACW58.1b M00078111 Baz1b/+(locked) Male 12.6 31 70 25 28 40 58
MACW68.1b M00078120 Baz1b/+(locked) Male 12.5 25 58 37 37 37 49
Baz1b/+ Average 12.66 26.8 61 29.2 31 37.6 46
Baz1b/+ Standard Deviation 0.14 4.49 10.39 6.46 5.02 6.95 6.84
Baz1b/+ Standard Error 0.06 2.01 4.65 2.89 2.24 3.11 3.06
MACW59.1d M00078109 Baz1b:failed(locked) Female 12.9 40 85 40 40 43 52
Baz1b:failed Average 12.9 40 85 40 40 43 52
Baz1b:failed Standard Deviation 0.0 0 0 0 0 0 0
Baz1b:failed Standard Error 0.0 0 0 0 0 0 0
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MACW33.1c M00078060 +/+(locked) Female 12.6 25 31 16 22 25 43
MACW61.1c M00078100 +/+(locked) Female 12.6 22 40 16 19 28 46
MACW72.1d M00078135 +/+(locked) Female 13.1 25 37 16 19 25 46
MACW51.1c M00078083 +/+(locked) Male 13.1 28 46 31 22 46 49
MACW56.1c M00078116 +/+(locked) Male 12.9 22 64 31 28 34 61
MACW59.1a M00078106 +/+(locked) Male 12.9 19 34 22 25 25 40
+/+ Average 12.87 23.5 42 22 22.5 30.5 47.5
+/+ Standard Deviation 0.21 2.87 10.91 6.71 3.2 7.63 6.65
+/+ Standard Error 0.08 1.17 4.45 2.74 1.31 3.12 2.72