Adult LacZ Expression Data for Cbx7 (MACK)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Cbx7 Knockout First D08 Cbx7 Knockout First D08
Genotype Cbx7/+ Cbx7/+
Gender Female Male
Age In Weeks 8.8 11.5
Adrenal Gland present present
Bone ambiguous ambiguous
Brain present present
Brown Adipose Tissue absent absent
Cartilage present present
Colon present present
Eye absent absent
Gall Bladder No data No data
Heart present present
Kidney ambiguous ambiguous
Large Intestine present present
Liver absent absent
Lung present present
Mammary Gland absent absent
Mesenteric Lymph Node present present
Nasal Epithelia ambiguous ambiguous
Oesophagus absent absent
Oral Epithelia present present
Ovaries absent No data
Oviduct present No data
Pancreas present present
Parathyroid present present
Peripheral Nervous System present present
Peyers Patch absent absent
Pituitary Gland No data No data
Prostate No data absent
Skeletal Muscle ambiguous ambiguous
Skin present present
Small Intestine present present
Spinal Cord present present
Spleen present present
Stomach present present
Testis No data present
Thymus absent absent
Thyroid absent absent
Trachea present present
Urinary System present present
Uterus present No data
Vas Deferens No data absent
Vascular System present present
White Adipose Tissue absent absent
Images
  • Tissue: Adrenal gland
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Knee joint
    Description: -
    Genotype: Heterozygous
  • Tissue: Large intestine
    Description: -
    Genotype: Heterozygous
  • Tissue: Liver and gall bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: Internal view
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype