Plasma Chemistry Data for Gys2 (MABV)

Fasted Plasma Chemistry (Ketamine / Xylazine)
Fasted Plasma Chemistry Metabolic Panel (week 15) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are fasted overnight (maximum 16 hours) then terminally anaesthetised with ketamine/xylazine, after which blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. The resulting plasma is analysed for glucose, triglycerides, cholesterol, high density lipoproteins, low density lipoproteins, non-esterified free fatty acids (NEFAC) and glycerol on an Olympus AU400. For a subset of genes, fructosamine is analysed using the AU400 and plasma insulin concentration is measured by ELISA and/or Mesoscale Discovery (MSD) array technology.
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Glucose
Triglycerides
Cholesterol
High Density Lipoprotein
Low Density Lipoprotein
NEFAC
Glycerol
Plasma Chemistry (Ketamine / Xylazine) - significant parameters
Plasma Chemistry Panel (week 16) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Non-fasted mice are terminally anaesthetised with ketamine/xylazine, and blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. Plasma is analysed for the following parameters on an Olympus AU400:
Electrolytes: Sodium, potassium, chloride
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Sodium
Potassium
Chloride
Glucose view graph MP:0005560 - decreased circulating glucose level
Fructosamine
Triglycerides
Cholesterol
High Density Lipoprotein
Low Density Lipoprotein
NEFAC
Glycerol
Insulin (MSD)
Amylase
Alanine Aminotransferase
Alkaline Phosphatase
Creatine Kinase
Aspartate Aminotransferase
Total Bilirubin
Total Protein
Albumin
Creatinine
Urea
Calcium
Magnesium
Iron
Phosphate
Lactate Dehydrogenase
Uric Acid
Plasma Chemistry (Ketamine / Xylazine) - significant parameters
Plasma Chemistry panel (week 14) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted mice are terminally anaesthetised with ketamine/xylazine and blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. Plasma is analysed for the following parameters on an Olympus AU400:
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Sodium
Potassium
Chloride
Glucose view graph MP:0005560 - decreased circulating glucose level
Fructosamine
Triglycerides
Cholesterol
High Density Lipoprotein
Low Density Lipoprotein
NEFAC
Glycerol
Amylase
Alanine Aminotransferase
Alkaline Phosphatase
Creatine Kinase
Aspartate Aminotransferase
Total Bilirubin
Total Protein
Albumin
Creatinine
Urea
Calcium
Magnesium
Iron
Phosphate
Lactate Dehydrogenase
Uric Acid