Phenotyping Data for Brd7 (MAAB) Associated with 'behavior/neurological' (MP:0005386)

Rotarod: Standard Protocol
Rotarod (week 10) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Mice are assessed for motor activity and coordination by rotarod (Bioseb). Three 5 minute trials on a rod constantly accelerating from 4-40rpm are performed with a 15 minute inter-trial interval, and the latency and reason for ending each trial is recorded. The trial is ended when the mouse either falls off the rod, jumps off the rod, or performs a passive rotation (the mouse grasps the surface of the rod and rides around the rod instead of walking on it).
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Latency to Fall Trial 1
Latency to Fall Trial 2
Latency to Fall Trial 3
Hot Plate: Standard Protocol
Hotplate (week 12) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Mice are assessed for thermal pain perception using a hotplate (TSE systems). Each mouse is placed on the plate heated to 52?C, and the latency to the first response, and response type, are recorded. Mice that do not respond after 30 seconds are removed.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Latency to respond
Open Field: Open Field (20 minutes)
Open Field (week 9) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Anxiety and exploratory drive are assessed over 20 minutes using open field apparatus (PhenoMaster ActiMot; TSE Systems)
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Total Distance Travelled Whole Arena
Whole Arena Resting Time
Whole Arena Average Speed
Total Rears
Total Distance Travelled - Periphery
Total Rest Time at Periphery
Total Time at Periphery
Average Speed at Periphery
Total Distance Travelled - Centre
Total Resting Time in Centre
Total Time in Centre
Average Speed at Centre
Latency To Enter Centre
Number Entries to Centre
% Distance Moved in Centre - Total
% Time Spent in Centre - Total
Indirect Calorimetry: Standard Protocol
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Water bottles are weighed before and after the experiment to assess water consumption.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Cumulative Food Intake
X-Activity Total
X-Activity Ambulatory