Auditory Brainstem Response (ABR) measurement for Brd7tm2a(EUCOMM)WtsiMGP Pipeline 2

Results Summary

  • ABR thresholds normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean Threshold is plotted (+/- population standard deviation). The green shaded area indicates the mean threshold (+/- 1SD and +/- 2SD) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and are plotted as a function of absolute sound pressure level (SPL) and SPL relative to threshold. Means are plotted (+/- population standard deviation).

ABR Thresholds Summary

ABR Thresholds for Brd7<tm2a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
MAAB19.1b M00013165 Brd7/+(locked) Female 13.8 37 13 19 34 43
MAAB19.1c M00013166 Brd7/+(locked) Female 13.8 64 25 25 40 49
MAAB22.1c M00013179 Brd7/+(locked) Female 13.6 55 22 34 37 61
MAAB23.1c M00013183 Brd7/+(locked) Female 12.7 43 13 19 34 52
MAAB6.1c M00013169 Brd7/+(locked) Female 12.7 52 22 31 37 52
MAAB8.1c M00013175 Brd7/+(locked) Female 14.0 37 19 22 34 49
MAAB8.1d M00013176 Brd7/+(locked) Female 14.1 43 19 22 37 52
MAAB10.1a M00013186 Brd7/+(locked) Male 13.5 58 28 40 43 55
MAAB10.1c M00013188 Brd7/+(locked) Male 13.5 55 22 25 34 49
MAAB13.1c M00013197 Brd7/+(locked) Male 13.5 49 22 28 34 49
MAAB13.1d M00013198 Brd7/+(locked) Male 13.5 55 22 25 34 58
MAAB24.1a M00013191 Brd7/+(locked) Male 13.5 58 31 34 43 64
MAAB6.1a M00013167 Brd7/+(locked) Male 13.5 52 28 31 31 46
Brd7/+ Average 13.52 50.62 22 27.31 36.31 52.23
Brd7/+ Standard Deviation 0.4 8.03 5.13 6.13 3.56 5.7
Brd7/+ Standard Error 0.11 2.23 1.42 1.7 .99 1.58
Controls
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
621.6d M00014134 +/+ Female 13.2 52 13 22 40 58
621.6e M00014135 +/+ Female 13.1 58 16 25 37 46
621.6f M00014136 +/+ Female 13.1 49 19 19 34 52
621.6g M00014137 +/+ Female 13.2 52 19 31 34 55
621.6h M00014138 +/+ Female 13.1 49 19 25 34 46
669.3a M00014124 +/+ Male 13.8 52 16 19 28 46
669.3b M00014125 +/+ Male 13.8 61 28 31 43 61
669.3c M00014126 +/+ Male 13.8 52 22 28 40 46
669.3d M00014127 +/+ Male 13.8 52 13 13 28 49
669.3e M00014128 +/+ Male 13.8 46 16 19 28 43
+/+ Average 13.47 52.3 18.1 23.2 34.6 50.2
+/+ Standard Deviation 0.33 4.12 4.25 5.56 5.16 5.72
+/+ Standard Error 0.1 1.3 1.34 1.76 1.63 1.81