Adult LacZ Expression Data for Brd7 (MAAB)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Brd7 Preconditional E04 (clone #5) Brd7 Preconditional E04 (clone #5) Brd7 Preconditional E04 (clone #5) Brd7 Preconditional E04 (clone #5)
Genotype Brd7/+ Brd7/+ Brd7/+ Brd7/+
Gender Male Female Male Female
Age In Weeks 8.5 8.5 11.4 11.2
Adrenal Gland present present absent absent
Bone ambiguous ambiguous absent absent
Brain present present present absent
Brown Adipose Tissue No data absent absent No data
Cartilage present present present absent
Colon present present No data absent
Eye absent absent No data absent
Gall Bladder No data No data No data No data
Heart present present absent absent
Kidney present present present absent
Large Intestine present present absent absent
Liver absent absent absent absent
Lung present present present absent
Mammary Gland absent absent absent absent
Mesenteric Lymph Node present absent absent absent
Nasal Epithelia present present present absent
Oesophagus present No data absent absent
Oral Epithelia absent absent absent absent
Ovaries No data present No data absent
Oviduct No data present No data absent
Pancreas present absent absent absent
Parathyroid No data No data No data No data
Peripheral Nervous System present present present absent
Peyers Patch present absent present absent
Pituitary Gland No data No data No data No data
Prostate ambiguous No data absent No data
Skeletal Muscle present present absent absent
Skin present present present ambiguous
Small Intestine present present absent absent
Spinal Cord present present present absent
Spleen absent absent absent absent
Stomach present present present absent
Testis ambiguous No data present No data
Thymus absent absent present absent
Thyroid No data No data No data No data
Trachea present No data present absent
Urinary System present present present absent
Uterus No data present No data absent
Vas Deferens ambiguous No data ambiguous No data
Vascular System ambiguous ambiguous present absent
White Adipose Tissue absent absent No data absent
Images
  • Tissue: Bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Hind limb
    Description: -
    Genotype: Heterozygous
  • Tissue: Kidney
    Description: -
    Genotype: Heterozygous
  • Tissue: Peyer's patch
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Trachea
    Description: Ventral view
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype