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Mammalian Phenotype
Chromosome
Marker Symbol

Browsing Data by Mammalian Phenotype: 'cardiovascular system'

Ccdc134

Ccdc134 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0001544 | MP:0002128 | MP:0001914
mp - MP:0005385

Gene Details

Marker Name(s): coiled-coil domain containing 134
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 2310042L06Rik, AW208859
Location: Chr15:82127922-82142200(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1923707 Ensembl Gene ID: ENSMUSG00000068114 Vega Gene ID:
Entrez Gene ID: 76457 CCDS ID:
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human CCDC134 79879
Rat Ccdc134 500909

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Ccdc134tm1a(KOMP)Wtsi MERW C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Ccdc134tm1a(KOMP)Wtsi MERW C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor Ccdc134tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MERW EPD0293_1_D11 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/Harwell
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 25305)
order order view details

ES Cell Clones

Cassette Type 7 Knockout-First Alleles 3 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0293_1_B12 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_C12 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_D11 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_E10 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_F09 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_F10 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_G10 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0293_1_C09 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_C10 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0293_1_G09 PRPGS00081_A_B10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
82648 PRPGS00081_A_B10 PCS00081_B_B10 L1L2_Bact_P L3L4_pD223_DTA_spec view
82648 PRPGS00081_A_B10 PCS00081_B_B10 L1L2_Bact_P L3L4_pD223_DTA_spec view
Vectors ES Cells
KOMP-Regeneron
(Project: VG14903)
order order view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 5 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
14903A-A2 VG14903 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
14903A-A3 VG14903 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
14903A-D10 VG14903 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
14903A-G1 VG14903 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
14903A-G10 VG14903 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
VG14903 ZEN-Ub1 view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
34 12 0 2 view in Ensembl

Cdh23

Cdh23 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0001544 | MP:0004085 | MP:0001629 | MP:0005333
mp - MP:0005385

Gene Details

Marker Name(s): cadherin 23 (otocadherin)
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 4930542A03Rik, USH1D, ahl, bob, bus, mdfw, nmf112, nmf181, nmf252, sals, v
Location: Chr10:60302748-60696490(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1890219 Ensembl Gene ID: ENSMUSG00000012819 Vega Gene ID: OTTMUSG00000017511
Entrez Gene ID: 22295 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human CDH23 64072
Rat Cdh23 114102

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Cdh23 MBTS view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Cdh23 MBTS view view view view view view view view view view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
EUCOMMToolsCre
(Project: 125079)
currently unavailable currently unavailable view details

ES Cell Clones

Cassette Type 7 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotorless
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
CEPD0036_4_B07 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
CEPD0036_4_B10 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
CEPD0036_4_C10 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
CEPD0036_4_D07 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
CEPD0036_4_F07 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
CEPD0036_4_G08 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
CEPD0036_4_H08 ETGRD0009_A_1_B03 C57BL/6N JM8.N4 tm1(EGFP_CreERT2)Wtsi Cre Knock In (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
40485 ETGRD0009_A_1_B03 ETPCS0009_A_1_B05 pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro L3L4_pD223_DTA_T_spec view
Vectors ES Cells
EUCOMMTools
(Project: 125417)
currently unavailable currently unavailable view details

ES Cell Clones

Cassette Type 30 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
MEPD1008_2_A03 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_A12 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_B04 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_B08 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_B09 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_C03 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_C07 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_C08 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_C11 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_C12 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_D05 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_D06 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_D09 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_E05 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_E06 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_E07 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_E09 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_E10 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_E12 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_F03 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_F04 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_F06 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_G03 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_G11 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_H01 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_H03 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_H04 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_H05 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_H10 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
MEPD1008_2_H12 PGM4044_Z_2_A01 C57BL/6N-Atm1Brd/a JM8A3.N1.C2 tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP pass LACZ pass
Chromosome 1 - Chromosome 8a fail Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
40485 PGM4044_Z_2_A01 PCS04044_B01 L1L2_Bact_P L3L4_pD223_DTA_T_spec view
Vectors ES Cells
EUCOMM
(Project: 31758)
order order view details

ES Cell Clones

Cassette Type 4 Knockout-First Alleles 3 Targeted Non-Conditional Alleles
Promotorless
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0320_1_A12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR pass
EPD0320_1_B12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR pass
EPD0320_1_D12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0320_1_F12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0320_1_C12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0320_1_E12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0320_1_G12 PGS00026_B_D01 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
40485 PGS00026_B_D01 PCS00026_B_D01 L1L2_st0 L3L4_pZero_DTA_kan view
40485 PGS00026_B_D01 PCS00026_B_D01 L1L2_st0 L3L4_pZero_DTA_kan view
Vectors ES Cells
KOMP-Regeneron
(Project: VG15164)
order order view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 6 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
15164A-B8 VG15164 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
15164A-D11 VG15164 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
15164A-D4 VG15164 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
15164A-F12 VG15164 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
15164A-G5 VG15164 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
15164A-H1 VG15164 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
VG15164 ZEN-Ub1 view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
62 24 14 2 view in Ensembl

Cenpj

Cenpj was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0002127 | MP:0005391 | MP:0002925 | MP:0001614 | MP:0002092 | MP
mp - MP:0005385

Gene Details

Marker Name(s): centromere protein J
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 4932437H03Rik, Gm81
Location: Chr14:56526761-56571846(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:2684927 Ensembl Gene ID: ENSMUSG00000064128 Vega Gene ID: OTTMUSG00000019769
Entrez Gene ID: 219103 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human CENPJ 55835
Rat Cenpj 305909

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Cenpjtm1a(EUCOMM)Wtsi MBKA C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Cenpjtm1a(EUCOMM)Wtsi MBKA C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view view view pdf view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor Cenpjtm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MBKA EPD0028_7_G05 C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view data
about )
WTSI/VETMEDUNI
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
EUCOMM
(Project: 34777)
order order view details

ES Cell Clones

Cassette Type 13 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotorless
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0028_7_A05 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_A06 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_C06 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_D04 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_E04 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ fail
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a pass Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_E05 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a pass Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_E06 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP pass LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a pass Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_F05 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_G04 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_G05 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a pass Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_G06 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_H04 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP pass LACZ pass
Chromosome 1 - Chromosome 8a pass Chromosome 8b -
Chromosome 11a pass Chromosome 11b - Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0028_7_H06 PGS00019_A_G09 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
39937 PGS00019_A_G09 PCS00019_A_G09 L1L2_gt1 L3L4_pZero_kan view
Vectors ES Cells
KOMP-Regeneron
(Project: VG17791)
order order view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 5 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
17791A-C11 VG17791 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
17791A-C8 VG17791 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
17791A-D6 VG17791 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
17791A-F3 VG17791 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
17791A-H6 VG17791 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
VG17791 ZEN-Ub1 view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
28 9 2 1 view in Ensembl

Chd7

Chd7 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0002127 | MP:0005391 | MP:0002925 | MP:0001614 | MP:0002092 | MP
mp - MP:0005385

Gene Details

Marker Name(s): chromodomain helicase DNA binding protein 7
view this gene in MGI
Marker Type: protein coding gene
Synonyms: A730019I05Rik, Cycn, Cyn, Dz, Edy, Flo, GENA 47, GENA 60, Gena 52, Lda, Mt, Obt, Todo, WBE1, Whi
Location: Chr4:8690406-8867659(+)
Related Human Conditions (from OMIM) - no related Human Condition

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Chd7 FL view
Chd7 MAMF view view view view view view view view view view view view view view view view view view view view
Chd7tm2a(EUCOMM)Wtsi MAWN 129S5/SvEvBrd/Wtsi;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Chd7 FL view view
Chd7 MAMF view view view view view view view view view view view view view view view view view view view view view view view view pdf view view
Chd7tm2a(EUCOMM)Wtsi MAWN 129S5/SvEvBrd/Wtsi;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor Chd7tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MAWN EPD0019_1_D07 129S5/SvEvBrd/Wtsi;C57BL/6N view data
about )
WTSI/CNB
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
EUCOMM
(Project: 35714)
order order view details

ES Cell Clones

Cassette Type 9 Knockout-First Alleles 8 Targeted Non-Conditional Alleles
Promotorless
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0019_1_A09 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_B09 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_C09 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_D07 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_E07 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_F08 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_G09 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_H07 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_H08 PGS00021_B_F02 C57BL/6N JM8 parental tm2a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0019_1_A07 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_B08 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_C07 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_C08 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_D08 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_D09 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_E08 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0019_1_G08 PGS00021_B_F02 C57BL/6N JM8 parental tm2e(EUCOMM)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
35837 PGS00021_B_F02 PCS00021_A_F02 L1L2_st0 L3L4_pZero_DTA_kan view
35837 PGS00021_B_F02 PCS00021_A_F02 L1L2_st0 L3L4_pZero_DTA_kan view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
45 8 5 4 view in Ensembl

Crlf3

Crlf3 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0002127 | MP:0005391 | MP:0002925 | MP:0001614 | MP:0002092 | MP
mp - MP:0005385

Gene Details

Marker Name(s): cytokine receptor-like factor 3
view this gene in MGI
Marker Type: protein coding gene
Synonyms: BB164954, Creme9, cytor4
Location: Chr11:80046493-80080991(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1860086 Ensembl Gene ID: ENSMUSG00000017561 Vega Gene ID: OTTMUSG00000000187
Entrez Gene ID: 54394 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human CRLF3 51379
Rat Crlf3 54395

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Crlf3tm1a(KOMP)Wtsi MADG C57BL/6Dnk or C57BL/6NTac;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Crlf3 MLFC view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Crlf3tm1a(KOMP)Wtsi MADG C57BL/6Dnk or C57BL/6NTac;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view view view view pdf view view
Crlf3 MLFC view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
currently unavailable Crlf3tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MFQB EPD0033_3_A11 C57BL/6NTac;C57BL/6NTac;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment
currently unavailable Crlf3tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MADG EPD0033_3_C11 C57BL/6Dnk or C57BL/6NTac;C57BL/6Brd-Tyrc-Brd;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 38131)
order order view details

ES Cell Clones

Cassette Type 11 Knockout-First Alleles 2 Targeted Non-Conditional Alleles
Promotorless
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0033_3_A11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_B11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_B12 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_C11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_C12 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_D12 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_E11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_F11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_G11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_G12 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_H12 PGS00023_E_B01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0033_3_D11 PGS00023_E_B01 C57BL/6N JM8.F6 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0033_3_E12 PGS00023_E_B01 C57BL/6N JM8.F6 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
38187 PGS00023_E_B01 PCS00023_E_B01 L1L2_gt0 L3L4_pZero_DTA_kan view
38187 PGS00023_E_B01 PCS00023_E_B01 L1L2_gt0 L3L4_pZero_DTA_kan view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
29 7 1 2 view in Ensembl

Csrp2bp

Csrp2bp was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0002127 | MP:0005391 | MP:0002925 | MP:0001614 | MP:0002092 | MP
mp - MP:0005385

Gene Details

Marker Name(s): cysteine and glycine-rich protein 2 binding protein
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 2510008M08Rik, ATAC2, AU023459, D2Ertd473e, D2Wsu131e
Location: Chr2:144368983-144407676(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1917264 Ensembl Gene ID: ENSMUSG00000027425 Vega Gene ID: OTTMUSG00000003524
Entrez Gene ID: 228714 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human CSRP2BP 57325
Rat Csrp2bp 362224

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Csrp2bptm1a(KOMP)Wtsi MBDX C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Csrp2bptm1a(KOMP)Wtsi MBDX C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view view view view pdf view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor Csrp2bptm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) MBDX EPD0025_4_B07 C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 38090)
order order view details

ES Cell Clones

Cassette Type 19 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotorless
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0025_4_A07 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_A09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_B07 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_B08 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_B09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_C07 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_C08 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_C09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_D08 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_D09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_E09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_F07 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_F08 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_G07 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_G08 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_G09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_H07 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_H08 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0025_4_H09 PGS00023_A_D10 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotorless Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
35277 PGS00023_A_D10 PCS00023_E_D10 L1L2_gt1 L3L4_pZero_DTA_kan view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
20 4 2 1 view in Ensembl

Cyfip2

Cyfip2 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0001544 | MP:0002128 | MP:0001914
mp - MP:0005385

Gene Details

Marker Name(s): cytoplasmic FMR1 interacting protein 2
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 1500004I01Rik, 6430511D02Rik, AA930218, AU022376, Pir121
Location: Chr11:46193855-46312294(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1924134 Ensembl Gene ID: ENSMUSG00000020340 Vega Gene ID: OTTMUSG00000005492
Entrez Gene ID: 76884 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human CYFIP2 26999

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Cyfip2tm1a(EUCOMM)Wtsi MEPG C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Cyfip2tm1a(EUCOMM)Wtsi MEPG C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor Cyfip2tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MEPG EPD0436_2_B03 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/VETMEDUNI
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
EUCOMM
(Project: 33461)
order order view details

ES Cell Clones

Cassette Type 3 Knockout-First Alleles 3 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0436_2_A04 PG00068_Z_2_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0436_2_B03 PG00068_Z_2_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0436_2_H02 PG00068_Z_2_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0436_2_E01 PG00068_Z_2_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0436_2_F03 PG00068_Z_2_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0436_2_G04 PG00068_Z_2_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
48341 PG00068_Z_2_A04 PCS00068_A_A04 L1L2_Bact_P L3L4_pD223_DTA_spec view
48341 PG00068_Z_2_A04 PCS00068_A_A04 L1L2_Bact_P L3L4_pD223_DTA_spec view
Vectors ES Cells
KOMP-Regeneron
(Project: VG18339)
order order view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 2 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
18339A-G6 VG18339 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
18339A-H2 VG18339 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
VG18339 ZEN-Ub1 view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
34 6 6 2 view in Ensembl

Dbn1

Dbn1 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0002127 | MP:0005391 | MP:0002925 | MP:0001614 | MP:0002092 | MP
mp - MP:0005385

Gene Details

Marker Name(s): drebrin 1
view this gene in MGI
Marker Type: protein coding gene
Synonyms: drebrin A, drebrin E2
Location: Chr13:55473429-55488111(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1931838 Ensembl Gene ID: ENSMUSG00000034675 Vega Gene ID: OTTMUSG00000017050
Entrez Gene ID: 56320 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human DBN1 1627
Rat Dbn1 81653

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Dbn1tm1a(KOMP)Wtsi MCKS C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Dbn1 MUCL view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Dbn1tm1a(KOMP)Wtsi MCKS C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view
Dbn1 MUCL view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor Dbn1tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MCKS EPD0211_3_A05 C57BL/6NTac;C57BL/6NTac;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR fail
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR pass
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 40214)
order order view details

ES Cell Clones

Cassette Type 7 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0211_3_A05 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0211_3_A07 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 0.9 - 0.81 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0211_3_A07 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0211_3_C08 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 1 - 0.91 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR pass
EPD0211_3_D07 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0211_3_F05 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 1 - 0.91 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0211_3_G05 PRPGS00070_C_D05 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
49236 PRPGS00070_C_D05 PCS00070_B_D05 L1L2_Bact_P L3L4_pD223_DTA_T_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
22 8 1 1 view in Ensembl

Ddx55

Ddx55 was found for the following reasons:
mp_id - MP:0005385
MP - MP:0005385
mp_ontology - MP:0000001 | MP:0005385 | MP:0001544 | MP:0002128 | MP:0001914
mp - MP:0005385

Gene Details

Marker Name(s): DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 2810021H22Rik
Location: Chr5:124552864-124569660(+)
Related Human Conditions (from OMIM) - no related Human Condition

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Ddx55tm1a(EUCOMM)Wtsi METX C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Ddx55tm1a(EUCOMM)Wtsi METX C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are avai