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Browsing Data by Available Products: 'mirKO ES Cells'

Mir9-1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mir9-1 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details

Marker Name(s): microRNA 9-1
view this gene in MGI
Marker Type: miRNA gene
Synonyms: Mirn9, Mirn9-1, mmu-mir-9-1
Location: Chr3:88215598-88215686(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:2676911 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: 387133 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
mirKO
(Project: mirKO20911)
order from MMRRC
order from EUMMCR
view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 4 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
mirKO_ES_PuDtk_3G10 mirKO_TV_PuDtk_PL1H4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_3G12 mirKO_TV_PuDtk_PL1H4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_3G8 mirKO_TV_PuDtk_PL1H4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_3G9 mirKO_TV_PuDtk_PL1H4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
49885 mirKO_TV_PuDtk_PL1H4 PGK_EM7_PuDtk_bGHpA PL611 view

DNA Clone Resources no data available

Mir9-2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mir9-2 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details

Marker Name(s): microRNA 9-2
view this gene in MGI
Marker Type: miRNA gene
Synonyms: Mirn9-2, mir 9-2, mmu-mir-9-2
Location: Chr13:83738814-83738885(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:3619442 Ensembl Gene ID: ENSMUSG00000065424 Vega Gene ID:
Entrez Gene ID: 723967 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
mirKO
(Project: mirKO20899)
order from MMRRC
order from EUMMCR
view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 5 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
mirKO_ES_PuDtk_4B1 mirKO_TV_PuDtk_PL2A5 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_4B3 mirKO_TV_PuDtk_PL2A5 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_4B4 mirKO_TV_PuDtk_PL2A5 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_4B5 mirKO_TV_PuDtk_PL2A5 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_4B6 mirKO_TV_PuDtk_PL2A5 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
49997 mirKO_TV_PuDtk_PL2A5 PGK_EM7_PuDtk_bGHpA PL611 view

DNA Clone Resources no data available

Mir9-3

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mir9-3 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details

Marker Name(s): microRNA 9-3
view this gene in MGI
Marker Type: miRNA gene
Synonyms: Mirn9-3, mir 9-3, mmu-mir-9-3
Location: Chr7:79505264-79505353(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:3619443 Ensembl Gene ID: ENSMUSG00000093238 Vega Gene ID:
Entrez Gene ID: 723968 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
mirKO
(Project: mirKO20829)
order from MMRRC
order from EUMMCR
view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 5 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
mirKO_ES_PuDtk_2B1 mirKO_TV_PuDtk_PL1D9 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_2B2 mirKO_TV_PuDtk_PL1D9 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_2B3 mirKO_TV_PuDtk_PL1D9 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_2B4 mirKO_TV_PuDtk_PL1D9 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_2B6 mirKO_TV_PuDtk_PL1D9 C57BL/6N JM8.F6 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
49927 mirKO_TV_PuDtk_PL1D9 PGK_EM7_PuDtk_bGHpA PL611 view

DNA Clone Resources no data available

Mir92b

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mir92b was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details no data available

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

Mir96

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mir96 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details no data available

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Mir96 MAMC view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Mir96 MAMC view view view view view view view view view view view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

Mirlet7a-2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mirlet7a-2 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details

Marker Name(s): microRNA let7a-2
view this gene in MGI
Marker Type: miRNA gene
Synonyms: Mirnlet7a-2, let 7a-2, mmu-let-7a-2
Location: Chr9:41536716-41536811(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:3619049 Ensembl Gene ID: ENSMUSG00000092770 Vega Gene ID:
Entrez Gene ID: 723965 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
mirKO
(Project: mirKO25860)
order from MMRRC
order from EUMMCR
view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 6 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
mirKO_ES_PuDtk_13B1 mirKO_TV_PuDtk_PL7-F3 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_13B2 mirKO_TV_PuDtk_PL7-F3 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_13B3 mirKO_TV_PuDtk_PL7-F3 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_13B4 mirKO_TV_PuDtk_PL7-F3 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_13B5 mirKO_TV_PuDtk_PL7-F3 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_13B6 mirKO_TV_PuDtk_PL7-F3 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
49945 mirKO_TV_PuDtk_PL7-F3 PGK_EM7_PuDtk_bGHpA PL611 view

DNA Clone Resources no data available

Mirlet7d

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mirlet7d was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details

Marker Name(s): microRNA let7d
view this gene in MGI
Marker Type: miRNA gene
Synonyms: Mirnlet7d, mmu-let-7d
Location: Chr13:48536012-48536114(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:2676796 Ensembl Gene ID: ENSMUSG00000065453 Vega Gene ID:
Entrez Gene ID: 387247 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
mirKO
(Project: mirKO20815)
order from MMRRC
order from EUMMCR
view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 4 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
mirKO_ES_PuDtk_9G12 mirKO_TV_PuDtk_PL5B1 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_9G7 mirKO_TV_PuDtk_PL5B1 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_9G8 mirKO_TV_PuDtk_PL5B1 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_9G9 mirKO_TV_PuDtk_PL5B1 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
210039 mirKO_TV_PuDtk_PL5B1 PGK_EM7_PuDtk_bGHpA PL611 view

DNA Clone Resources no data available

Mirlet7f-1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mirlet7f-1 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details

Marker Name(s): microRNA let7f-1
view this gene in MGI
Marker Type: miRNA gene
Synonyms: Mirnlet7f1, mmu-let-7f-1
Location: Chr13:48537829-48537917(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:2676798 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: 387252 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Vectors ES Cells
mirKO
(Project: mirKO20921)
order from MMRRC
order from EUMMCR
view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 5 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
mirKO_ES_PuDtk_8G10 mirKO_TV_PuDtk_PL4D4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_8G12 mirKO_TV_PuDtk_PL4D4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_8G7 mirKO_TV_PuDtk_PL4D4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_8G8 mirKO_TV_PuDtk_PL4D4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
mirKO_ES_PuDtk_8G9 mirKO_TV_PuDtk_PL4D4 C57BL/6N-Atm1Brd/a JM8A3 Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen - 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
210454 mirKO_TV_PuDtk_PL4D4 PGK_EM7_PuDtk_bGHpA PL611 view

DNA Clone Resources no data available

Mirlet7f-2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Mirlet7f-2 was found for the following reasons:
ikmc_project_product_status_str - mirKO ES Cell Available

Gene Details no data available

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available