The gene family encoding protein kinases is the most commonly mutated
in human cancer. Moreover, mutated and activated protein kinases have
proved to be tractable targets for the development of new anticancer
therapies. We have examined the full coding sequence of the protein kinase
genes (518 protein kinases, more than 1.3Mb of DNA per sample) in primary
cancers and cancer cell lines. The genes with mutations and the details of
the mutations are available through COSMIC. In addition a list of genes
without mutations is available.
To complement the sequencing of the protein kinase genes we have analysed the
tumours and cell lines using Affymetrix SNP microarrays. This data provides a
genotype for each sample and reveals regions where there is loss of heterozygosity.
Furthermore we have analysed the array data for changes in genome copy number by
comparing the tumour data with data from a normal sample (CGH).
The results from this work have been published and incorporate a discussion of
the data. There are various supplementary data files that are available to
support the data. These include details of the samples, the genes that have been
screened and the mutations. The oligonucleotide PCR/sequencing primer sequences
are also available.