Wolbachia pipientis

The Sanger Institute has been funded by Beowulf Genomics to sequence the genome of the Wolbachia pipientis endosymbiont of Culex quinquefasciatus, in collaboration with Dr. Steven Sinkins of the Department of Zoology, University of Oxford and Prof. Scott O'Neill of the Dept. of Zoology and Entomology The University of Queensland, Australia.

Culex quinquefasciatus is a vector of human Bancroftian filariasis. The mosquito contains an endosymbiotic bacterium, Wolbachia pipientis, the genome of which is estimated to be 1.5 Mb, and will be sequenced with a whole-genome shotgun approach.

Finishing is in progress; a database containing the latest assembly is available for searching on our Blast Server , or for download from our FTP site.

At present there are 12 contigs > 1kb (12 contigs >2kb) with a total size of 1.546 Mb

The shotgun reads are also available: There are 24,531 reads totalling 13.243 Mb and giving a theoretical coverage of 99.74% of the genome.

Finishing warning

Please note that although finishing has begun, the assembly database may still contain errors and misassemblies, and E. coli and vector contamination. The contig numbers are not stable, and will vary with each data release. The sequences in the shotgun database are single reads from ABI sequencers; they will contain errors, along with both E. coli and vector contamination.

Data Release Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale.

Sequencing enquiries

Please address all sequencing enquiries to: parkhill@sanger.ac.uk

webmaster@sanger.ac.uk

Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK Tel:+44 (0)1223 834244

Last Modified Thu Jan 31 09:01:10 2008

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