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Plasmid sequencing

The Sanger Institute Pathogen Genomics group is sequencing a number of plasmids from various sources. Sequence data for each project will be made available for download (FTP site) and searching (BLAST server) once assemblies have begun. The annotation will be made available upon publication. Please read our data release policy and guidelines and conditions on use of data.

Plasmid information
Plasmid Source Size Phenotype Status Data
pBtoxis Bacillus thuringiensis 137 kb insecticidal toxins Published: Berry et al., Appl Environ Microbiol 68, 5082-5095 (2002) Website
pCoo Enterotoxigenic E. coli 98.4 kb CS1 pilus Published: Froehlich et al., J. Bacteriol. 187 6509-6516 (2005) Website
R478 Serratia marcescens 286 kb Antibiotic resistance Published: Gilmour et al.,
Plasmid 52, 182-202 (2004)
Website
pHag1 and pHag2 Bacteroides fragilis strain WI1 5.6 kb and 9.9 kb Antibiotic resistance Finished FTP site

In collaboration with Christopher Thomas (University of Birmingham), John Wain (Sanger Institute) and the broader plasmid biology community (with advisors Anne Summers, Athens Georgia, USA and Ron Skurray, Sydney, Australia) we are sequencing reference plasmids for each of the Escherichia coli incompatibility groups for which no complete sequence exists, along with equivalent plasmids from modern clinical isolates. These are all listed below. If you have a plasmid that might be interesting to sequence in this context please contact Christopher Thomas. The sequencing of plasmid pVL501 is in collaboration with Jonathan Fletcher (University of Bradford).

Plasmid information
Plasmid Size Inc group NCTC# Resistances Status Data
RA1 129 kb IncA/C 50073 Su Tc Planned -
R667 168 kb IncA/C 50463 Ap Su Finished Sequence
TP113 86 kb IncB=O 50084 Km Hg Finished Sequence
TP114 62 kb IncIbeta(2) 50085 Km Planned -
R387 80 kb IncK 50022 Cm Sm Finished Sequence
R471 78 kb IncL/M 50036 Ap Hg Finished Sequence
pIP135 68 kb IncL/M 50107 Gm Sm Su Tc Hg Finished Sequence
N3 50 kb IncN 50124 Sm Sp Su Tc Hg [HspII] In Finishing -
R753 102 kb IncV 50521 Ap Cm Sm Su Planned -
R7K 30 kb IncW 50006 Ap Sm Sp Published: Revilla et al.,
Antimicrob Agents Chemother. 52, 1472-1480 (2008)
Sequence
pIP71 68 kb Com9 50106 Ap Cm Sm Sp Su Tc Finished Sequence
R6K 39 kb IncX 50005 Ap Sm Finished Sequence
R485 54 kb IncX 50040 Su Planned -
pHH1508a 208 kb IncH - Sm Sp Tp Tel Phi In Finishing -
MIP233 231 kb IncHI3 - Scr Phi Tel In Finishing -
pLV501 96.5 kb IncFII - Cm Km Tc Hg Finished Sequence

Legend

Ap - ampicillin; Su - sulphonamide; Gm - gentamycin; Cm - chloramphenicol; Tc - tetracycline; Tp - trimethoprim; Sm - streptomycin; Sp - spectinomycin; Km - kanamycin; Hg - mercury resistance; Tel - tellurite resistance; Phi - resistance to various bacteriophages; Scr - sucrose fermentation; [ ] square brackets - restriction-modification system


Data Release Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale.


Finishing warning

Please note that although finishing has begun, the assembly database may still contain errors and misassemblies, and E. coli and vector contamination. The contig numbers are not stable, and will vary with each data release. The sequences in the shotgun database are single reads from ABI sequencers; they will contain errors, along with both E. coli and vector contamination.


Sequencing enquiries

Please address all sequencing enquiries to: parkhill@sanger.ac.uk

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Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK  Tel:+44 (0)1223 834244

Last Modified Wed Oct 22 15:52:32 2008

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