The Project
The Wellcome Trust Sanger Institute Pathogen Sequencing Unit is undertaking partial shotgun sequencing of a number of different species of Plasmodium. This will serve as useful comparative genomics resource to complement the almost complete Plasmodium falciparum genome. This work is being done in collaboration with Chris Newbold (Oxford) and Mike Turner (Glasgow).
The Strain
We sequenced the H strain of P. knowlesi. The H strain originally came from a naturally infected patient and was acquired in peninsular Malaysia in 1965.
Genomic libraries were constructed from P.knowlesi total genomic DNA in the pUC18 vector. Two ligations have been used to produce a total of 8x coverage of the genome. Genomic DNA was provided by Clemens Kocken (BPRC).
Progress
The genome of P. knowlesi (H strain) is published in Pain et al, Nature 455, 799-803 (2008). See the press release for further details.
Accessing the P. knowlesi Sequence data
Sequence data can be downloaded from the FTP site.
Searching the P. knowlesi genomic sequence online
You can also search against P. knowlesi genomic sequence using our online BLAST server.
