The Sanger Institute has been funded by the BBSRC to sequence the genome of Pseudomonas fluorescens, in collaboration with Dr. Paul Rainey of the Department of Plant Sciences, University of Oxford, and Prof. Chris Thomas of the Department of Biosciences, University of Birmingham.
Pseudomonas fluorescens encompasses a diverse group of bacteria that is commonly found in a wide range of terrestrial and aquatic habitats. We will be sequencing strain SBW25; a plant-growth promoting bacterium that colonises both plant leaves and roots.
The sequence is finished, and is available for searching on our Blast Server, or for download from our FTP site.
The genome is now published in Silby et al., Genome Biology 2009 10:R51. The full annotation is available in the EMBl database with accession number AM181176.
The genome is 6,722,540 bp in length, with an average G+C content of 60.5 %
The shotgun data is still available: At present there are 126,206 reads totalling 57.041 Mb and giving a theoretical coverage of 99.98% of the genome.
We have also sequenced pQBR103, a plasmid from P. fluorescens. The plasmid sequence is now published in Tett et al., ISME J. 2007 1, 331-340. The full annotation is available in the EMBl database with accession number AM235768. The sequence is 425,094 bp with a G+C content of 53.15%. The data is available for searching and download as above.
