The Project
The Wellcome Trust Sanger Institute Pathogen Sequencing Unit is undertaking partial shotgun sequencing of a number of different species of Plasmodium. This will serve as useful comparative genomics resource to complement the complete Plasmodium falciparum genome. This work is being done in collaboration with Chris Newbold (Oxford) and Mike Turner (Glasgow).
Library construction
Genomic libraries were constructed by Chris Jansen from P.chabaudi AS strain total genomic DNA in the pUC18 vector, with procedures to remove the murine DNA. The insert size for the library is 2-4kb. Both ends of the inserts have been sequenced to give approximately 8x genome coverage
Project Status
As part of a large scale survey of the Plasmodium lifecyle an initial survey of the P. chabaudi genome has been published based on analysis of a ~3x partial genome shotgun.
Whole genome shotgun sequencing has continued to 8x coverage. Finishing (gap closure) was started in 2008 and will be followed by a publication of the completed and annotated genome sequence.
The latest 8x assembly sequence data can be downloaded from the FTP site.
Searching the P. chabaudi genomic sequence online
The P. chabaudi genome is available online through GeneDB.
You can also search against P. chabaudi genomic sequence using our online BLAST server.
