The Sanger Institute has been funded to sequence the 4.4 Mb genome of Mycobacterium bovis strain AF2122/97 (spoligotype 9), in collaboration with Dr. Stewart Cole at the Unit de Genetique Moleculaire Bacterienne, Institut Pasteur and Dr. Glyn Hewinson at the Veterinary Laboratories Agency.
M. bovis is the major cause of tuberculosis in cattle and other animals, and can be transmitted to man. The strain chosen is a fully virulent UK isolate from a cow suffering caseous lesions in lung and bronchomediastinal lymph nodes. A matched isolate from a badger found in the cattle shed is also available.
The sequence and analysis has now been published: Garnier T et al., The complete genome sequence of Mycobacterium bovis P.N.A.S. early edition (2003)
It was generated with shotgun reads from the Sanger Institute with finishing and analysis carried out at the Institut Pasteur.
The genome is 4,345,492 bp long, with a G+C content of 65.63%. The sequence and annotation data is available for download by FTP and can be searched using our BLAST server.
The sequence and annotation have been submitted to EMBL and Genbank with the accession number BX248333The annotation will shortly be available from the Institut Pasteur's Genolist server.
As an extension to this project, we have produced a 4x-coverage shotgun sequence of Mycobacterium bovis BCG Pasteur, the vaccine strain of M. bovis. It was also generated with shotgun reads from the Sanger Institute with finishing and analysis carried out at the Institut Pasteur.
The genome is now finished, and the complete sequence is available for searching on our Blast Server, or for download from our FTP site.
The genome is 4,375,192 bp long, with a G+C content of 65.64%.
The sequence and analysis has now been published in Brosch et al, PNAS 104, 5596-5601, 2007.
The sequence and annotation have been submitted to EMBL and Genbank with the accession number AM408590.
The shotgun data is still available: there are 32,982 reads in the database, totalling 17.418 Mb and giving a theoretical coverage of 98.09% of the genome. The data is available for download by FTP and can be searched using our BLAST server.
Other sites that may be of interest:
The TubercuList TB database at the Institut Pasteur, Paris
Sanger M. tuberculosis and M. leprae web sites
