What are the chances of identifying a non-sense or splice affected mutation in my chosen gene?

At present, we routinely screen ~500bp of coding sequence per gene across 3840 individuals, which yields ~1/3 chance of identifying a nonsense or splice-site mutation. The actual rate depends on the amino acid sequence of the protein (some have greater probability of producing to a nonsense allele with ENU mutagenesis than others).

How long will the screening take?

Each library of F1 fish from a given ENU mutagenesis is screened for ~12 months. Therefore, requests could take up to 12 months to process. If a mutation is not found, requests are retained until the next library of F1 carriers is available.

How long will it take to receive fish for alleles I request?

4-6 weeks (as long as the fish are laying well, and the allele is available for request, i.e we have identified carriers)

Can other labs ask for alleles in genes I requested?

Yes, we are an open resource, and release all lines to the community.

Are requests anonymous?

Yes

Could I get adults?

No, we are only able to ship fertilized eggs.

Want more information?

email mr-help@sanger.ac.uk