clone sequencing and manual annotation

Clones from different libraries were ordered by fingerprinting and by locating markers from the SATMAP, HS, MGH and T51 maps. A minimal tiling path was identified and clones were selected for high quality sequencing (gold standard). The sequence data is submitted to ENA/Genbank

The resulting tiling path (TPF) is used for the generation of integrated assemblies which contain the clone sequences plus added whole genome shotgun sequence to fill remaining gaps.

The finished clone sequence is also run through an analysis pipeline that includes repeatmasking, ab initio gene prediction and blast searches against all available protein, EST and cDNA data. The information generated is then used to manually annotate the clone adding gene structures, descriptions and poly-A features. After this, the clone is submitted to EMBL/Genbank again and can also be browsed in Vega.

		clones browse the live clone paths and download sequence in Chromoview blast against all zebrafish clones status of a clone in the sequencing pipeline details about the used clone libraries information about EMBL submissions ftp EMBL clone sequence files gene annotation contains the sequenced clones on chromosomal tile paths as determined by the mapping project. The clones are manually annotated, resulting in high quality gene structures based on supporting evidence. The project is ongoing and Vega is updated frequently. browse manual gene annotation in Vega request manual annotation of your region of interest