clone mapping and sequencing project

For the clone based mapping and finishing, clones from different libraries were fingerprinted. An fpc database was built with the resulting information, thereby linking overlapping clones into FPC contigs. Subsequently, clones from a tiling path through the FPC contigs are being selected for sequencing, resulting in high quality sequence which is submitted to EMBL/Genbank.

The finished clone sequence is then run through an analysis pipeline that includes repeatmasking, ab initio gene prediction and blast searches against all available protein, EST and cDNA data. The information generated is then used to manually annotate the clone adding gene structures, descriptions and poly-A features. After this, the clone is submitted to EMBL/Genbank again and can also be browsed in Vega.

		mapping project overview mapping contacts browse the live clone paths and download sequence in Chromoview details about the used libraries gene annotation Vega contains the sequenced clones on chromosomal tile paths as determined by the mapping project. The clones are manually annotated, resulting in high quality gene structures based on supporting evidence. The project is ongoing and Vega is updated frequently. You can ask for a gene/region of your interest to be manually annotated. search You can blast against all clones on the chromosomal tile path in Vega or against all the zebrafish clones that were sequenced (or are currently being sequenced) at the Sanger Institute status information status of a clone in the sequencing pipeline download information about EMBL submissions ftp EMBL files other ask for a clone of your choice to be sequenced