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Retrieve BLAST result
Zebrafish WebFPC information

WebFPC official users guide

The official WebFPC users guide from the Arizona Genomics Insitute descibes all the standard features of the Contig Selection Screen and the Contig Display screen.


Sanger reports

Reports are available for clones and markers shown in the map display area, which are accessible via the information lookup control (described in the Getting Additional Information section in the guide to the Contig Display Screen). To access a report click on the clone or marker of interest to highlight it and then select "Look up in current .." from the information lookup control area of the Contig Display Screen, as shown below.

Sanger information lookup control

A clone report, please see example DKEY-110K5, gives library information, the external clone name and sanger sequencing pipeline information for selected clones (coloured grey, orange or yellow in the default map display). If a clone is selected for sequencing a linkage group assignment may be given, this is normally based on e-PCR results for a clone sequence in the fingerprint contig or related WGS contigs and therefore may only be considered a "best guess" based on data available at the time. If the clone has been end sequenced these will be detailed in the End Sequence Report section of the page.

You can also search for a fingerprinted clone, by Sanger clone name, external clone name or sequence accession via the clone report interface.


A marker report, please see example stID310085.9, gives basic information for a marker. Reports for publically available markers normally contain links to the site from which we collected the data e.g. UniSTS, other marker types e.g. "faux markers" represent primers designed only to place sequences by e-PCR and will not be found in external databases. The marker names shown in the map are normally in the following standard format :

Placement prefix Sanger id Linkage group (optional)
et = placed by e-PCR,
st = placed by another method
internal sanger id for a pair of primers assignment by external laboratory

e.g. etID18021.5, stID17506

Markers names not in the standard format have normally been used for specific small scale projects, or have been provided to us with no primer/sequence information. Please contact us if you have a specific query regarding a particular marker.

We use the Tübingen radiation hybrid map as our framework map in FPC, so a marker report for markers on this map will also contain the radiation hybrid map position.

The clone marker relationships table lists markers associated with clone. The relationship types are as follows :-

Relationship type Description
0 The marker is found in WGS contig by e-PCR which has been aligned to this region (approx. +/- 150kb). There is no direct relationship with the clone and this data is covered by all the same caveats as the WGS contigs.
1 The marker is designed in an end sequence read from this clone.
2 The marker is found by e-PCR in the sequence of this clone.



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Last Modified Mon Sep 27 12:40:43 2004

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