An international consortium which includes the Sanger Institute has completed the sequencing of the Dictyostelium discoideum genome (strain AX4). D.discoideum has a 34 Mb haploid genome with a base composition of 77% [A+T]. It consists of 6 chromosomes encoding approximately 12,500 proteins.
Publication details
| The genome of the social amoeba Dictyostelium discoideum. Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A Nature. 2005;435;43-57. PMID: 15875012 |
Dictyostelium discoideum is a soil amoeba widely used as a model organism in cell and developmental biology. The amoeba shares many physiological functions seen in mammalian cells and is amenable to genetic manipulation.
Resources and useful links
- DictyBase:
Resources for the Dictyostelium research community
- D. discoideum cDNA project
consortium pages
- Ted
Cox
- The National Institutes of Health model organism
initiative and Dictyostelium,
contains links to other Dicty sites
Dictyostelium discoideum Sequencing Projects links
- Baylor College of
Medicine in Houston
- GSC in Jena
Sequence Data
Sequence data and preliminary analysis data can be accessed through GeneDB. Sequence data for the genome are deposited in the EMBL nucleotide database under accession number AAFI00000000. The six chromosomal assemblies have accession numbers CM000150 to CM000155, and their component sequence contigs have accession numbers AAFI01000001-AAFI01000336.
