10th February 2000: Sequence and annotation of C.jejuni NCTC 11168 complete  The complete sequence and annotation of C. jejuni NCTC 11168 has been published.  Press Release  Nature Paper  Sequence Data

"The genome sequence of the foodborne pathogen Campylobacter jejuni reveals hypervariable sequences"
Nature 403, 665-668 (2000).

J. Parkhill, B.W. Wren, K. Mungall, J.M. Ketley, C. Churcher, D. Basham, T. Chillingworth, R.M. Davies, T. Feltwell, S. Holroyd, K. Jagels, A.V. Karlyshev, S. Moule, M.J. Pallen, C. W. Penn, M.A. Quail, M-A. Rajandream, K.M. Rutherford, A.H.M. van Vliet, S. Whitehead, and B.G. Barrell.

The complete sequence is 1,641,481 bp in length (with 25 polymorphic regions) and was generated from 33,824 sequencing reads. It starts at the first base in the dnaA gene, close to the origin of replication. Both the sequence and annotation have been deposited in the public databases with the ID CJ11168 (accession number AL111168).

C. jejuni is the leading cause of food poisoning, being three times more common than Salmonella. Further information on C. jejuni can be found on our collaborators' website.

The genome sequence of C. jejuni was produced in collaboration with Brendan Wren of the London School of Hygiene and Tropical Medicine and Dr. Julian Ketley of the Department of Genetics, University of Leicester. It was sequenced using a whole genome shotgun approach using several 1.4-2.2 kb pUC18 libraries, both generated in-house, and supplied by Andrey V. Karlyshev from Dr. Wren's laboratory.

In August 2006, Ozan Gundogdu from The London School of Hygiene & Tropical Medicine in collaboration with the Sanger Institute, carried out a complete re-annotation of the NCTC11168 genome. The re-annotation has added new information to over 1450 of the original 1654 coding sequences with over 300 product functions being updated. Additional Campylobacter work carried out in Brendan Wren's group can be accessed from the Campylobacter Resource Facility homepage

		C. jejuni Data    The complete updated annotation as an EMBL file for viewing in Artemis.    A hierarchical list of genes, classified by function.    A list of stable RNAs present in the sequence.    A table of polymorphic regions present in the sequence.    A BLAST server allowing searching of the sequence and predicted CDSs.    FTP access to the sequence and CDS databases.    Sequence and annotation in Entrez Genomes at the NCBI.    The complete gene map coloured by function, A4 or A3 sized, in PDF format

We have also generated sequence data from C. jejuni M1 using the 454/Roche GS20 system.

The sequence is in 624 contigs with a total size of 1.619 Mb and may be downloaded from our ftp site.

Please note that this sequence is unchecked and unedited, and will contain errors. The GS20 is known to have problems with homopolymeric tracts, and these are therefore likely to contain significant numbers of errors.