Burkholderia pseudomallei

Sanger Institute Press Release Image source: Copyright Dennis Kunkel Microscopy, Inc.

The Sanger Institute has recently published the 7.2 Mb genome of Burkholderia pseudomallei in collaboration with Dr. Rick Titball of the Defence Science and Technology Laboratory ,Porton Down, and Dr. Ty Pitt of the Heath Protection Agency. We have sequenced strain K96243, a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University.

The genome is 7,247,547 bp in size with a G+C content of 68.06% consisting of two chromosomes of 4,074,542 bp and 3,173,005 bp.

		B. pseudomallei Data     The complete Chromosome 1 sequence and annotation in Artemis format.     The complete Chromosome 2 sequence and annotation in Artemis format.     Chromosome 1 tandem repeats (greater than 12 bp) in Artemis format.     Chromosome 2 tandem repeats (greater than 12 bp) in Artemis format.     A BLAST server allowing searching of the chromosome sequences and predicted CDSs.     FTP access to the sequences and CDS databases.       ACT comparison file of B. pseudomallei chromosome 1 to chromosome 2 (BLASTN, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 1 to B. mallei chromosome 1 (Genbank:CP000010) (BLASTN, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 1 to B. mallei chromosome 2 (Genbank:CP000011) (BLASTN, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 1 to R. solanacearum chromosome (EMBL:AL646052) (TBLASTX, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 1 to R. solanacearum megaplasmid (EMBL:AL646053) (TBLASTX, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 1 to P. aeruginosa genome (EMBL:AE004091) (TBLASTX, filters: score 100).       ACT comparison file of B. pseudomallei chromosome 2 to B. mallei chromosome 2 (Genbank:CP000011) (BLASTN, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 2 to B. mallei chromosome 1 (Genbank:CP000010) (BLASTN, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 2 to R. solanacearum megaplasmid (EMBL:AL646053) (TBLASTX, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 2 to R. solanacearum chromosome (EMBL:AL646052) (TBLASTX, filters: score 100).     ACT comparison file of B. pseudomallei chromosome 2 to P. aeruginosa genome (EMBL:AE004091) (TBLASTX, filters: score 100).

The databases are available for searching on our BLAST server, and for download from our FTP site

The annotation for the B. pseudomallei genome is now accessible through GeneDB. GeneDB allows searching, browsing and download of the full annotation.

Reference:

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei" Proc. Natl. Acad. Sci. USA 101: 14240-14245 (2004).

M.T.G. Holden, R.W. Titball, S.J. Peacock, A.M. Cerdeño-Tárraga, T. Atkins, L.C. Crossman, T. Pitt, C. Churcher, K. Mungall, S.D. Bentley, M. Sebaihia, N.R. Thomson, N. Bason, I.R. Beacham, K. Brooks, K.A. Brown, N.F. Brown, G.L. Challis, I. Cherevach, T. Chillingworth, A. Cronin, B. Crossett, P. Davis, D. DeShazer, T. Feltwell, A. Fraser, Z. Hance, H. Hauser, S. Holroyd, K. Jagels, K. E. Keith, M. Maddison, S. Moule, C. Price, M.A. Quail, E. Rabbinowitsch, K. Rutherford, M. Sanders, M. Simmonds, S. Songsivilai, K. Stevens, S. Tumapa, M. Vesaratchavest, S. Whitehead, C. Yeats, B. G. Barrell, P.C.F. Oyston and J. Parkhill

Other sites that may be of interest:

• Heath Protection Agency B. pseudomallei facts sheet

• B. mallei strain database at TIGR

• B. pseudomallei Multi Locus Sequence Typing database

This project is funded by Beowulf Genomics

Sequencing enquiries

Please address all sequencing enquiries to: parkhill@sanger.ac.uk