Library Construction

The ESTs (Expressed Sequence Tags) were derived from a cDNA Library supplied by collaborators at The Department of Infectious Diseases and Immunology of Utrecht University. The cDNA was prepared from mRNA extracted from an asynchronous in vitro culture of B. bovis strain C61411, in bovine erythrocytes. The cDNA was then cloned into EcoRI/XhoI sites of UniZap lambda vector (Stratagene) and then excised to pBluescript for sequencing.

Sequencing and Database Information

The sequences contained in the single EST database are unedited single reads from ABI sequencers; they will contain errors, along with E. coli and vector contamination. The clustered database contains automatically aligned unfinished contigs that may include errors and ambiguities. Numbers in the clustered databases are not stable, and will vary with each data release. The databases are available from our FTP site or can be searched using the BLAST server.