CRISPR GUARD is a tool to reduce off-target editing by Cas9 and base editors.

Short guide RNAs called "GUARD RNAs" recruit Cas9 complexes to off-target sites but do not permit nuclease activity, thereby protecting them from the mismatched guide RNA by direct competition.

The CRISPR GUARD Finder tool searches for guide RNA off-targets and designs GUARD RNAs to protect them from editing.

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please cite: Coelho et al., CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs, Nature Communications, 2020
Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

Shiny app development by Matt Coelho, CRISPR GUARD Finder code by Mike Firth. With thanks to Nick Boughton.