Gene Editing and Cellular Research and Development | Cellular Operations

Gene Editing and Cellular Research and Development | Cellular Operations

Gene Editing and Cellular Research and Development

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Our Research and Approach

We develop novel genome editing techniques, cellular differentiation and cellular phenotyping systems, especially with respect to high-throughput investigation of gene and non-coding regulatory element function. 

  • Developing induced pluripotent stem cell models of genetic disease
  • Genetic screening techniques using pooled and arrayed CRISPR/Cas9
  • Differentiation protocols of pluripotent stem cells to brain and blood-derived lineages
  • Application of screening to complex phenotypic readouts such as single cell transcriptomics or spatial genomics 
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People

Bassett, Andrew
Dr Andrew Bassett
Group Leader
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Alumni

Key Projects, Collaborations, Tools & Data

Partners and Funders

Internal Partners
External Partners and Funders

Publications

  • Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes.

    Bruntraeger M, Byrne M, Long K and Bassett AR

    Methods in molecular biology (Clifton, N.J.) 2019;1961;153-183

  • Predicting the mutations generated by repair of Cas9-induced double-strand breaks.

    Allen F, Crepaldi L, Alsinet C, Strong AJ, Kleshchevnikov V et al.

    Nature biotechnology 2018

  • In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering.

    Wu Q, Ferry QRV, Baeumler TA, Michaels YS, Vitsios DM et al.

    Nature communications 2017;8;1;2109

  • Alpha-synuclein induces the unfolded protein response in Parkinson's disease SNCA triplication iPSC-derived neurons.

    Heman-Ackah SM, Manzano R, Hoozemans JJM, Scheper W, Flynn R et al.

    Human molecular genetics 2017;26;22;4441-4450

  • Editing the genome of hiPSC with CRISPR/Cas9: disease models.

    Bassett AR

    Mammalian genome : official journal of the International Mammalian Genome Society 2017;28;7-8;348-364

  • Precision Modulation of Neurodegenerative Disease-Related Gene Expression in Human iPSC-Derived Neurons.

    Heman-Ackah SM, Bassett AR and Wood MJ

    Scientific reports 2016;6;28420

  • A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells.

    Bassett AR, Kong L and Liu JL

    Journal of genetics and genomics = Yi chuan xue bao 2015;42;6;301-9

  • Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.

    Bassett AR, Azzam G, Wheatley L, Tibbit C, Rajakumar T et al.

    Nature communications 2014;5;4640

  • Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9.

    Bassett AR, Tibbit C, Ponting CP and Liu JL

    Biology open 2014;3;1;42-9

  • Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.

    Bassett AR, Tibbit C, Ponting CP and Liu JL

    Cell reports 2013;4;1;220-8