Cellular Operations

Cellular Operations

Cellular Operations

Our Approach

The Cellular Operations team provides cell biology expertise and support to enable the delivery of projects across the various scientific programmes at the Sanger Institute.

Cellular Operations at the Wellcome Sanger Institute

Cellular Operations is led by Alex Alderton and consists of approximately 60 scientists with specialist skills and experience across a number of key technology platforms:

Induced Pluripotent Stem (iPS) Cells

We generate human iPS cells from blood and skin samples and differentiate those pluripotent cells into a range of different cell types.  Current protocols include macrophage, cortical and dopaminergic neurons. This work is performed by our Cellular Generation and Phenotyping team.

Organoids

We have expertise in the derivation and culture of 3D organoid lines from human tissue samples.  In particular the team have extensive experience the generation of organoid models from tumours to develop new cellular models for cancer research. This work is performed by our Cellular Generation and Phenotyping team.

CRISPR Screening

We carry out large scale CRISPR-Cas9 screens in a range of different cell lines.  These screens allow investigators to study the impact of genetically altering thousands of genes in the genome in a single experiment. This work is performed by our Cellular Generation and Phenotyping team and Gene editing teams.

Genome engineering

We use highly efficient methods that take advantage of CRISPR-Cas9 technology to genetically engineer a range of cell types.  Current services offered include gene knockouts, SNP introduction, protein tags and vector construction.   This work is performed by our Gene editing team 

Flow Cytometry and Cell Sorting

Our Flow Cytometry team offer investigators state of art instrumentation and expertise in flow cytometry.  The facility is equipped with four high throughput cell analysers and five cell sorters. 

The majority of our work involves collaborating with other teams to carry out specific scientific projects.  In addition, we also undertake our own R&D to develop tools, establish new platforms and processes and to identify potential efficiency improvements. R&D is carried out by scientists within all of our teams as well as in our dedicated R&D team.

Research

Groups