Ms Caroline Tigoi

Visiting Worker

Alumni

This person is a member of Sanger Institute Alumni.

Caroline Tigoi is a visiting PhD student from University of Oxford undertaking a DPhil in clinical medicine. Her PhD project is focusing on acquisition and loss of carriage of antimicrobial resistant  among vulnerable children and neonates in Kenya. She is using epidemiological, biostatistical and genomics approaches characterise the frequency and longitudinal dynamics of acquisition and loss of AMR. Prior to starting he PhD, Caroline worked as the Labaratory lead for the CHAIN network being implemented in 9 sites in Africa and Asia, that aims to understand the pathways and causes of death in hospital and after discharge among young acutely ill children.She is an experienced microbiologist with great interest in emergining infectious diseases aiming to understand the clinical and genomic determinants of antimicrobial resistance and evaluating its impact on mortality among vulnerable children.

Acquisition and loss of carriage of antimicrobial resistant Enterobacterales among vulnerable children and neonates in Kenya: clinical and genomic determinants

I would like to evaluate the changes in bacterial genomic characteristics of antimicrobial resistance carriage over time using whole genome sequencing and phylogenetic clustering. I would like to know the types of AMR genes (ARGs) and virulence genes are carried at admission to hospital, acquired in hospital, and present in the hospital environment, sources of  acquisition of ARGS and genotypes associated with persistent carriage 6 months post discharge from hospital. My bacterial isolates have been sequenced using Illumina and nanopore platforms with the aims to identifying AMR genes using ARIBA and using De novo assembly and phylogenetic approaches to determine genetic relatedness of my strains at the gene/mobile genetic element/strain-level.

 

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