I use prime editing to induce large scale structural variants in human cells
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Really cool and creative work turning the BCL6 BTB domain into a small-molecule controlled, reversible protein oligomerization switch!
We employed the BTB domain of transcription factor BCL6 as small-molecule-controlled reversible oligomerization switch. Led by @lena_nitsch and my first corresponding author manuscript @CellRepMethods 1/16 https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(22)00048-0#.YlcELay7FD4.twitter
Our work is finally out @NatureBiotech! We functionally scored and interpreted nearly 1000 variants in the NPC1 gene using saturation prime editing and locus haploidization ✂️🧬 https://twitter.com/NatureBiotech/status/1495812638735052800
Saturation variant interpretation using CRISPR prime editing https://go.nature.com/3LOa1MK
Prime editing can be used to precisely insert short DNA sequences into the genome. But what determines how efficiently any given sequence can be inserted? Joined forces @JulianeWeller, Elin Madli Peets, Ananth Pallaseni, @FabioLib, @LeopoldParts https://www.biorxiv.org/content/10.1101/2021.11.10.468024v1. A 🧵