As staff scientists we perform basic research in Microbial Pathogenesis whilst also teaching relevant skills to students and visiting scientists. Our projects include growing and differentiating human induced pluripotent cells to a macrophage-like lineage and investigating host-pathogen interactions. The main techniques that are used are flow cytometry, con-focal imaging, high-throughput Cellomics assays and cell culture
Transcriptional profiling of macrophages derived from monocytes and iPS cells identifies a conserved response to LPS and novel alternative transcription.
Scientific reports 2015;5;12524
Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells.
Infection and immunity 2015;83;7;2926-34
Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.
Scientific reports 2015;5;8908
Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
PloS one 2012;7;12;e52232
The critical role of histone H2A-deubiquitinase Mysm1 in hematopoiesis and lymphocyte differentiation.
Mannosidase 2, alpha 1 deficiency is associated with ricin resistance in embryonic stem (ES) cells.
PloS one 2011;6;8;e22993
Defining the range of pathogens susceptible to Ifitm3 restriction using a knockout mouse model.
PloS one 2013;8;11;e80723
Epithelial IL-22RA1-mediated fucosylation promotes intestinal colonization resistance to an opportunistic pathogen.
Cell host & microbe 2014;16;4;504-16
Induced pluripotent stem cell derived macrophages as a cellular system to study salmonella and other pathogens.
PloS one 2015;10;5;e0124307
Signatures of adaptation in human invasive Salmonella Typhimurium ST313 populations from sub-Saharan Africa.
PLoS neglected tropical diseases 2015;9;3;e0003611
Infection by Brucella melitensis or Brucella papionis modifies essential physiological functions of human trophoblasts.
Cellular microbiology 2019;21;7;e13019
Using a Systems Biology Approach To Study Host-Pathogen Interactions.
Microbiology spectrum 2019;7;2
A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions.
PLoS neglected tropical diseases 2016;10;2;e0004446