High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
 
       
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by
Valid After Annotation
not done
Comment Type Comment Detail Edited User Edited Date
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 96147
Chr: chr14-02
Strand: 1
Status: Ordered
BACs: RP23-389P9 : RP23-442L4 : RP23-161M3 : RP23-206J5 :
Instance: Plate 132: Well D08: BACs RP23-161M3 RP23-442L4 RP23-389P9 RP23-206J5
Oligos on GRCm38:
Type Start End Length Seq
G5 65971326 65971375 50 GGGATGTTTGCATGTTTCGGGAGAGGTATCTTTAGTATTTGGCATGTTAA
U5 65972808 65972857 50 TGAATGCAGCTCTTCATTTATCGTCCCGTCTCCATGGTGTCTGGTGCTAT
U3 65972960 65973009 50 ATGCTTAGGAGGTTGTTTTGTTTTGTTTTCTGTTTTCCTTGAAATATTCT
D5 65973636 65973685 50 GAGCCTCAGTGCCGAGGCCTTGAATTTGGTTTCCCTGCAACTCTGGTCCA
D3 65973697 65973746 50 CTAGATTCATTTCAATAGGTTTTTCTAACCCTTGCTGTGGGTAGGGCATG
G3 65980685 65980734 50 AAAAAAACCGAGATAGTTTTGCTCGGAATTCTCTAGGCATCCAGAAATGA
Oligos:
Type Start End Length Seq
G5 64925436 64925485 50 GGGATGTTTGCATGTTTCGGGAGAGGTATCTTTAGTATTTGGCATGTTAA
U5 64926918 64926967 50 TGAATGCAGCTCTTCATTTATCGTCCCGTCTCCATGGTGTCTGGTGCTAT
U3 64927070 64927119 50 ATGCTTAGGAGGTTGTTTTGTTTTGTTTTCTGTTTTCCTTGAAATATTCT
D5 64927746 64927795 50 GAGCCTCAGTGCCGAGGCCTTGAATTTGGTTTCCCTGCAACTCTGGTCCA
D3 64927807 64927856 50 CTAGATTCATTTCAATAGGTTTTTCTAACCCTTGCTGTGGGTAGGGCATG
G3 64934795 64934844 50 AAAAAAACCGAGATAGTTTTGCTCGGAATTCTCTAGGCATCCAGAAATGA
G5_U5 64925436 64926967 1532 GGGATGTTTGCATGTTTCGGGAGAGGTATCTTTAGTATTTGGCATGTTAAATATGCTCTGGAGACACAGGAAATTCATCTGCCCTGCCTCAGGCTCTTATGGGAAGAAAGGGACTCAGCTTGGAGCTCGGCCCCTCCAACTCCAGATAGCCTGGCTCCTCTGTGCAGCCGGGCCATGGTTCTTCCCAGGGCCTCGCTGTCTCCAGTGTCTATAGAAGGGGTCAGTTTAATCTTGGCTTTCTTCCCCAGTTCTTGAGAAGTAGATTGCAGTGATGCCAGATGAGGAGACAGATGAGGGCCGCGTGTTCCAATCCCACCCCACCTCCCCTCTCAGGGAGCTGTAGCAGGGTGCAGAAAACCCTGAAGCTGCCCAGGGGTTGCCATGACACATCTGCTGTTGTTTCCAGGACTCCAGATTCCAAGGAGGCCACGCCATGAAGATTCTCCTGCTGTGCGTGGCACTGCTGCTGATCTGGGACAATGGCATGGTCCTGGGAGAGCAGGAGGTCTCTGACAATGAGCTCCAAGGTAGGTAGGCTGAGACTGTTCCTCTGGTGGATGCTATAGGGCCCAGGGCAGGGCCATTCCTCAAAGCTCAGTCCCCAAGTGCTGAGGGCTGTTTTCATTCTACATCCTGGGTCTGTCCATGCCCTCTGCCCTCTCTAGGTCCTGTCTTGATGTTTTTGTGGTCAGGTGACTTCTAGACATTGCCCTCTGTCTCAAGCCCCTCTGTTGGCTGCCTGTCATTCCTTTGTCATTACTGGTCCCCTCACCTGGCTCTCTTACTCTTCTCCCAAAGCCGTGCCCTAGACCCTTTGAAACTTTGTCAGTCTTCCCTTTGTGTGACATCCAGGGTTCTTACCGATGTGTCTCCACACCAACCCCTCCTGGAAAGCCATATCCACCAATTTCCTCCTCTGGAAAAAGGGCAATGCAATTTGACATGAAGTAGTCACCTGAGAGCACCCTCTCCCTTCCTCCCCATCTTCCAAGGCTTTTCTGGAAACCCACCTTTTCTCCCTTCTTCTCTCCTAACCTCTTGCTCATAGCTGACCAGTTCTGGGGGGGGGGGTCTTCGTTAAAGACAATGTGATCAGATTCTTTGATGTGGATACACACGGTGAAATGATTGCTGTTGTCAAGCTATTAGCTGTCCATCACCTCAACTTATCATTGGGTTCAGACACTTAAAATATGTTCTCTCAGCACACTTCAAGGGTAGAACACATTATCAACCACGAGCTCTGCTTGGACCATTGACCTTCGGGCTTACTTGTGTTGCTACATCATTGAAGCTCTTGCCCTTGGCTCAGCAGTCCCCCCTCCCGCTTCTCTTTGGATGTGTCTGGCAACCGCATCAGGTAGAAAACAGCAGAGGTGTGGGAAATGGCACTTGACACAGGATAAGTAAGTGGTACCACCGTGACATGATGTGTCATTGCCTTCGTCACCTGGGGCCGTGTCATGGCTATGGTCTCCATGGTGAATGCAGCTCTTCATTTATCGTCCCGTCTCCATGGTGTCTGGTGCTAT
U3_D5 64927070 64927795 726 ATGCTTAGGAGGTTGTTTTGTTTTGTTTTCTGTTTTCCTTGAAATATTCTCTGTGGCTGTTGGCTCTCCAGGGTGGCATGGTGGGGTCAGTCATAGTATGGCTGCCCCTCTCTGCACCACTCACTCCTGAGTTTCTGAGCTGTGACTTGGAGTCTACCATGAGCCCTTTGATGGGACATAGAGGCAGGACTGAGGCCATAAGGACTGCCACAGGCTTGGGGTAATAGTGATGGTGTCTCAGCTTGACGCCCCCAGCACTCCCCATCAAAGCATGGCTTTCTCACCGTCCCCCTGGCACCCAGGCTATTTCTCCAGCCATTCCTCCCCGCCCCCCTCAACTCACTGTACCTTCCTCTCTCTCACAGAACTGTCCACTCAAGGGAGTAGGTATATTAATAAGGAGATTCAGAACGCCGTCCAGGGAGTGAAGCACATAAAAACTCTCATAGAAAAAACCAACGCAGAGCGCAAGTCCTTGCTCAACAGTTTAGAGGAAGCCAAGAAGAAGAAAGAGGTAGGGCCAAGCCTTGGCTGAGCCCAGGCTCTCCCTGCTGGAGGGGAGGAGGGGACATGGTGAGCAGGTGCCTTTCTGGGTTGCCATGGTGTCCTAAGGCGGTCCTCGAAGGGTTCCGCTAATGCTGGACTGAGGAGCCAGGGAAAGCACATTCGCTTGTGGGAGCCTCAGTGCCGAGGCCTTGAATTTGGTTTCCCTGCAACTCTGGTCCA
D3_G3 64927807 64934844 7038 CTAGATTCATTTCAATAGGTTTTTCTAACCCTTGCTGTGGGTAGGGCATGGTGCAGGACTCAGCGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATATGAGCATCCAGACATTTCCCATTCTTTTCTTCTGTATTCAAAATGACTTTTAACATGAAAGTTCTTATTGAAATATTTTAAAATCAATGTAAAATTCTTGTAATCTATCCTTAGACATCCATCCATTCATTCATTCCCACTCAGATATAGGGGAGGGGGGCAGTTTTCAAAACCCTTAGTAGAAGCTGCAGTTGTGGCTAGCACCAGGCCCTGCAGAGATTGTGCTTCTGTAATTTATAAATTACAGACAGTAAGGTATGAGCAGCCACAATAAAATAGAGCAATTATAATGCTATACCAGTTATACCATACAGAAGGCTACGCAGATGTGATTTCTCCCACACAAAATATCTCAATTCCTCAGCTTCCACAATACTATTTTTAGACTGGTTGACCATGGATAGCTAAGAACTGATAACAAGGGACTGCCCTGTTACCATTTGTTTAATTTGTCATCTACATTTGAACATCAATGTTGACTCTGGTACACACACACACACACACACACACACACACACACACACACACACACAAACTTATCCACAGATTCATCCATCCACTTAATTATTTTCCATATTGATATGTAGACACACACATTTGCTATAAACTAGTATCTCTTCAGGCGGAGAATTTAAATTTTTCATTTTAGCAATCCCCAGAGCCAATGACTGGGTCAGGGGCATGGTAATGAGTTTGATGGTTGATTTGACTGGATAGCTTCGATTTGCATGTCAGAGGCCACAGTTCTAACAGAAAGATAAGACTGTCCTCTTTGGGGAGATGAGAGGAGACAGTGGGGTGGTTTAAAGGAGAGGTGGGGCCTAAGGAAGAGACGGTCAGAGCAGCAGATCAGAGTGGACAGGGAAGCTTAGGGAATGGCTGGGCCTTGAAGAAGGAGAGGCTCCTGAGAGCGTGGCTGGGGAGGCCCAACAGAAGGGCTAGAGCGAAGCTGTGGGCACCAGGAGCAACCTTGTACTCAGATCGGGGAGACCGGCTGGGTCTCTGTGTGGGTCACCTGGCCTTCTTGTCATCTCCCTGAAGGATGCTCTGGAGGACACTAGGGATTCTGAAATGAAGCTGAAGGCATTCCCGGAAGTGTGTAACGAGACCATGATGGCCCTCTGGGAGGAGTGCAAGCCCTGCCTGAAGCATACCTGCATGAAGTTCTATGCACGTGTCTGCAGGAGCGGCTCAGGGCTGGTCGGCCAGCAGGTGAGAAGGAGAGATGTCCACAGCCTGGGTTCTGGATGGGGAATGTGGAGAATCCATGAAATAGGCCTCATTCCATATGCTGGGTACAGTTGATTAGCATTGCTTGACTGCACATCGTTCTAGTGTCATGGTCGATTGGTGATGTCTGCTTGGCAGGTACCATCTCTTTCAAGACCTTTAACTGGTTGGGTAAAATGGCCTTATGGGATGGTAGAATCCTGTGTTAGAATAGGAAAAGGGAATTAAAAAATATTTAGTCTATTTGCATTAATTCTATCCCTACCTGTGAGATTCACTTGGTTGGAACTCAGTAAAATTCACTGTTTCCTCAAGGCTGAGCAGCTAAGCAGGAGTGGCTATTTATTCTTTATGGCCCAAGGTGAGACTTGGGAACCTCCGAGTTTCGCCATGAGAAATGGATTTGGGGGGCGGCGGCGGGGGGACGGGGGGTGCTGACAGGGTACCCAGGAGGCAGAACAGTGGGAACTTGGGTCAGTTTCTGTGTGGCCACAGGGGGATACAGGCTGGAGTACACACAATGCCTTACTCTACTTCTCTGGGTCCCAGCTAGAGGAGTTTCTAAACCAGAGCTCACCCTTCTACTTCTGGATGAACGGCGACCGCATCGACTCCCTGCTGGAGAGTGACCGGCAGCAGAGCCAAGTCCTGGATGCCATGCAGGACAGCTTTGCTCGGGCATCTGGCATCATAGACACGCTCTTTCAGGACCGGTTCTTCGCCCGTGAGCTTCATGACCCCCACTACTTCTCCCCCATCGGCTTCCCACACAAGAGGCCTCATTTCTTATATCCCAAGTCCCGCTTGGTCCGCAGCCTCATGTCTCCCTCCCACTATGGGCCTCCGAGCTTCCACAACATGTTCCAGCCTTTCTTTGAGATGATCCACCAGGCTCAACAGGCCATGGATGTCCAGCTCCACAGCCCAGCCTTCCAGTTCCCAGACGTGGATTTCTTAAGAGGTTAGAAAGCAATTGCTTTCTTTCTAAGTAGGAATGTGAATGTCCCAGATGTCTAGGCTCTTCCAGGGCTTGACCTGTGCTGGTTTTATGTTCCTAGAAGCCTCAGAGGCTGGTATTTCCTCCATTTGTAGGGTAAAGGAGCTAGGATGGGGGCAAAGAAATGCAGGTAAATGTGGGGGGGCGCAGTTCAATTAGCATTTGTAGAGATTGGAGCTCTTGTGTTTGTTACAGTGAACTCAGGGTGTGATCTGGGGCTGTGCCTCCTATAGGATCCCAGATATTATTTCTTGGGAACTTTTGAGGGTGTGGTGTGAGAGGCCCGCCCTTCCAGGCAATGAGTCTGTGTCATCTTAAAGAAGATGTTCATGGCTGTGACCTTAGCGTGTAATGCCTGTAAGGATGAAATATCACGTGGACACGAGAAACCGGTGTCTGAGGAGTGATTTTTTTTTTCCATTAGTGCCTTGGGTCTTAATACCATGTCCTACATGGTATGGTTTGCATTAGAAATAGAAGAAGTCTTGGCATCCAACACACATCTACTGTAGCTCCATTGTCTAAAGATGGTTTATGTTGTCAGGACACACTTGTGCTCTCTGGGGCTTGAAGGCCAGTTAGGGAGTACCATCAGTCAAGGAGAACAAAGAACAGCCATGATGTCAGAAGGCACACAGCTGAGGGGATGTGGCAGGAGAGATCTCCTCAGATGGAGATGGTGGGAAGGCAGGCAGGACCTGCTGAGAGCCGTGGGAATATCCTCAGCCACAGGGAAGTCCCATGGCTGCAAGCAAACAAATAAGCTGCATCGTGCCCTGACCATGGCACTGAGCAGAGGTGTGGAAGGCTGTCGGGTGGGCATCCTTCTGATGTGCCATGTGTCTAATCCTGCCCCTCTTTCCCCTCATGTGTCCAGAAGGTGAAGATGACCGCACTGTGTGCAAGGAGATCCGCCGCAACTCCACAGGATGCCTGAAGATGAAGGGCCAGTGTGAAAAGTGCCAGGAGATCTTGTCTGTGGGTGAGTCCGGGAACCAGTGGTCATCTGACTGTGGGTCTCCAGTGACTCACTGGCACTATCCTTTTCCAAAGACTAGCTCTCTGCCTGGGAACTCTTAATCCCCGGGAGACTCCTCACCCTGGTGCACAGCCTACTCTCCTCTTATCTAGAGCTTCATTATTTGGGAGCTGGGGTAGGACCCAGTGCCTCCTGCATGTTAGGTGAACGCTCTACCACTGAGCCACATCAATTTTACTCAGAATCAAGTTCAGGTGCAGATTGTTCTCATATAGATGAGGTGGAGCTAAAGTGCCCACCACCACCCCTGTGGCTCACTGGCAAACCTGCAGTCCACGGTGGAATTACACTGTATCGACCAGTAAGCCACAGCTCGTTCCCCTGGCTACTTTGTTCCACAGTGCCAAGTACTGTAGACCCTGATTTTGAACTTCTGATTATGAAATCTTTGACAACATGCGTTTTGTTTAACAAGTGCTCGCCACATGCCAGGCACTGCTGCAAACACGTCACAGGTGTCTGTTCGCAGAATGATCCTGTGAGCTGGGTACAGCTCTCTTTCCGTCTTGCTGGATGGCTAAGTGACTTATCTAAGATTACGTGACACGAGGCTTCTCACTTAGGCTGTGCTCATAAACAGCACCCTGGGGTTTCTCCCTGGGGACCTTGAGCTATGTCCTTGTGATGGTGGTCCTTGGTATCCTGGCCGCATTCTTTTTGGTGAACTAGTCCCTCAGAAAAGGAACTATTTTTCCTTCATCAGTTCCCCAGGCAATCTGGGTATGGTGACATATCCCTATAATCCCAACATTTGGGAGGCTGAGGTAGGAGGGTGGTGAATCCGAGGCCAGCGGCGAGGTTCTGTCTCAATGAAAACAAAACAAAATACAGCAAACCCAAAACTGTTCCCCACAGATTTGTCAGGTCTGGCTATCAAGCCAATGCCCCGCCTTCAGCCTGGTCTTTACAGTGGCTCACAGTACACGGTGCTACCTGCTCAGATCACCCTCTTTGTTGGCCGGGACAGACCTTTAGAGTCTGGTTAAATATTCCCAGCCTCATTCCTGCAATGTTAGGAGGCTAGGAAAAAAAAAAAAATACTAGTGCTTTCAAAGGTCATAGTGTGACATTATAAACAAATATGGGCCACACCTGTAGGCTTTCCCTTATAGTTGAAAGATGGGGTGCCTGAGCACAGAGAAGCCAGTCACCTTATAGACTGGATCTCTTACATCAGACTTAGCGATCCTCTCAACAGTCACCCGTTAGCTCTTATTTACGGTGCTTATAATGGTATCCTCTCAGCAGTCACCGTTTCTGTCACCCGTTAGCTCTTATGTACAGTGCTTACAATGGTATGGATAGGCTTCACAGAGACCTCTCGCCTGGGACATCTTGCCTCAGCCCTGTGGGCAGACCTGACCATGGCTGAGCATTTAACAACATTTTTGGCTCCAACCTACCATCTACCAAGAGCAACCCTCCCTCCACTGTGACAATCAGAAACATCTTCAGATTGTCTGGACTGTCTCCTGGTGGGTGGTGTGGTCCCAGGTTGAGAACCACCGTTCTACTCTGTCCTTCTCTCAGGACTTTCTTGTTTGGGCTAATCAGTGGTTAGAGTGCTCCGGTTATTCCTGGAAGATTTCTTCTCATTTCTGCTTCTGGTCTCCAATCCTGTGTCTTGCCCCATGCAACATTATATCTTATTTTAAGTATGAACAATTGTAGTAGCCACATAGGGCCCAGAAGGCATCTCTCTTGTCTTGCTTAGTTTGGATATAGCCTCTCATAGATGTTTAAAAAATAATTGAGGAGGGGGGAGGCGTTGTTAAACACTGGTTTTCAGAAAGCATGAGCACTTTGTTGGTCTCAGTAGCCTGATGCCTGCAGTGCACTCTTGCAGAAGAGAGGATGCCAGAGTTCCTATTTCCTCCTGGTCATCCTGGGTGGACAAGGGCAGGCAAGGACTGACCCTTGGGCACCAAACACCTGTGTTTTGTAGACTCGGGACCTGGCTGTCTCTTCTGCAGGTTTGGTTCTGGGAAAATGGTCTTGCCGAATCTGTCAAATTATGTTCAAAAATAAGTTTGGAGAATATTCTAGAACCCTGGTCTCAGGGGCTCAGGTTGAGGAGTGAAAGATGGGGCTTCAATCCTGGTTCCACAGATCGCTTCCTCTATAGATCTGAGCAAGGTCCACCTAAGTAAATAATGATCCTGTTCTTTGTGGGGTGTCCAGTGCCACAGTAGCTTCCTCCTATTTTGGAAATGAGTCCTGGGAGGAGCTCAGGGAGCAAGAGTAAGTCCAGGAACATGGGGTAGTCAGCATAGGTGCTCCCCAGAGCACCGTCAGCAGCATTGTGACAGGTCACCAAGACTGGTCATGTCTACCGCCCAAAGAAGACTCAGCCACGCTGCTCTGACACAGTTTAGCACAGCTAAGCCATTGTATCCCAAAGCACGAAGGGGGCAGCACCTTGGTTTCCGTGTCCGGGGAACTGTAAGAGCTGATCTGTAGCCCTCCCTGGAAGAGTTCCTCTGCTTCAGATTGTGGCTGCTGCAGCTGGTTCCCAGGGGCGTGTCCGTCCCCTTTACCCTCCCTTTCATGCTTCTGCCTTCTGCTTCTTTCTTCTCTGGGCAGACTGTTCAACCAACAATCCTGCCCAGGCTAACCTGCGCCAGGAGCTGAACGACTCGCTCCAGGTGGCCGAGAGGCTGACAGAGCAGTACAAGGAGCTGCTGCAGTCCTTCCAGTCGAAGATGCTCAACACCTCATCCCTGCTGGAGCAGCTGAACGACCAGTTCAACTGGGTGTCCCAGCTGGCTAACCTCACACAGGGCGAAGACAAGTACTACCTTCGGGTCTCCACCGTGAGTTGTATCCCAGTCATTTGATGAGGCTGAGACCCGGGTGTGATCCAGAGGTGATGTGTGTCTCTTAGAGATGTGGTGGGACAAAGGTCCCACCTAGGATTTACACCAGCAGGGACTAGGAAAACCGTATGCACGTTGTAGCATTGAAATGCCTTTGCCCCGTCAAATATCCAAGTCTCATTGTCAAGATGAGGAAAGAGAGAGGTTCTGTGGTTTGCTAAGAACAGACAGTTTTGGGCAACTTCCCTGGGTCAGCCTGTCAGCCTGGCTGTAAAGCTGCTTCCTGCCCAGGGCCACTCTGAGTGCCACATGAATTGGTTCACTGTTAGCCAGTGAGAACTCGGTTTCCTTTAGTTTGTGGACCCCACCCCTACCCCCTTTGACCACAGACTGGCTAACATTTGTACAAAGCAAATGTTGGGGCCAGGTGTTGTGGTGCGTGCCTGTAAGCCAGCACTGAAGAAACTGAGGCAGGAGAGTAGAGAGCTTGAGCCTGGGCTGGACTATATAGTGACAGCCTCTCTCAAACAAAGCAAAACAAGAGAACACAACAAGAAGAAAACATTCAAAAATCATTTCAAGTTTCTGGAGTGCCTACTTGGCTAGTCATTTTTAAGTAACATTAAAGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAAACAAACAAAGAAGCAAGCAAGCAAACAAACACACAAACAAAAAAACCGAGATAGTTTTGCTCGGAATTCTCTAGGCATCCAGAAATGA
U5_15 64911919 64926967 15049 TGACTGAAGCACAAGGAAGCTATCATGACTGCAGACACAGTTGAGGAAAAGGAACCCAAAAGGCTTCTGTGACAACCCCTACCTCCCCAAAGTAATAGCCTAGAGAGGAAACCATCAAGAATTAAAAAAAAAAAAAACCTAACAACAAACAAACAAACAAACAAACGGGGGGGTCAGGGGTTGCTGGTGGAGAAGGGTTCAGCTTCCCTGGAAAGGCTGGCCACTAGGAGTCTGACAATGCTTTGGTGAATGTATGAACAACTCACAATTGACTTCATTTCTCACCCCCTCCCCCTCCCCCTGCCCCTCCCCCCCCTCCTCCTCTTCTCTGTGTGTGCAGGGTAGATATGGGAGGTCTGGGGGGTGAGTACCATTGGGGGGCACGGTGTAAGATTCCCCAAAAGCAATAAGAATATTACGTTAAACAAAACGGTGAGTGAGATGGAGCATTGGCCTTTAGCTCTCGGCTTTGTGACCAGGGATCTAAAACTGCAGTCGCTGTGCCTTCTCCACACAATAGCCTATGCCCTCAAGTCTCAAGCAAAGCAAGCCCTTCCTCCTTGAAGTAGCTTTTGTCAGGGTATTTTATCATAGCAAATGGAAAAGCGACGACGACGACGACAGTTTCTCTGACAATAATGTCACATATTAGGTTATCAGTCTAGAATAAATAAAGCTAGACCACGCTCTTATCCCCACCCCCACTCCCACCACAGACACTCATACACAGAGTCAGATGCAAAGATTTCCTTTTGGTTAGATATATAGATGTACAAGCATAACACAGTGGAAGCTGAATGAAAATGAGGAAGATAGCTTTGAGCGGAAGGAAGAAATTTTCAAACAAGACAGGACACTCAGAGACTAAAGAAAAAGATAAACATGTTTACTTGCATAGAAATTTGAGAAAGCCTCCACAACCAATGGGGGGGGCGCAAATAAATAAATAAATGGCAGCTTGCTGGAAAGGGGGCTGAAGCCATGGCTCAGAGGTTAAAAGCAGTTGCTGCTCTTCCTGATCAAGTCTGGATTGTAGAACTCATGCCAAGCAGCTGACAACCACCCAGGAATCCAGCTCCAGGGCCCCTGACACCCTCCTCCACCTCTGAAGGTACGAGGCACACGCTCAAACATGCAAGACAAGTAAATAAAGATCAAAAAATAATTTTTAAAAGAAAGAAAATTTAGAACACACAAAAGATTACTTTCTTTGTCAATTCAAAAACTCTTAAGATTGATAGAAAAACACAAATAACTTGTTCAAATAAACGGAGAGCCAAGAGACAGAAAATGCCTGCCTCTTTGATTGCCTGTTTCTGTCTCTGTCTATTTGTGTGTCTGTTTCTCAGTTTCTGTGTCTCTGTGTCTCTTTCTGTTTCTCTGTCTCTGTATGTCTCTGTCTGTGTGTCTCTGTCTCTCTCCATGTGTCTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTCTCTGTGTTTTTTTATGAGGCAGGGCCTCATTCAAAGGCCCAGGCTGGCCTGACCTTGCTGTGTAGCTCAGGTTGGCCTCAAACTCATGATGCTAGCAGGCACACTGTTCTTGATAGAAGATGGTAGTTTTCAGCTCACCAAATGGTCCTGTGGGAAGGGACAGGCTTACAGAAGCTAAAGTCACAGAAATCCTTGGGGGAAGGTAAACAGACAGTGCCTTAAGAGTGAGCCCTAGGGGAATGCCAGGGCCAAGAAGCGGGAGTGGGTGGGTAGGGGAAGCAGGGTGGGGGGAGGTTATAGGAGACTTTTGGGATAGCATTTGAAATGTATATAAAGAAAATATCTAATAAAAGACTAAATTTAAAAAAAACAACCCCCCCCAAACAAAACAAAACAACAAAAAAAGAGTGAGCCCTGTGCAATGGATAAAATACTTGCTGTCCAGAAGAACAAACAAGGGCCAGAGTTTGGATTCCCAGAACTTACATCTGGATGTGGTCATGCCCATCTGAAGAGACAGGAGTGGATAGCCTGGTGTCTACAGCAGGGAACAAGAGATCTAGCCTTGAACAAGGTAGTTGGTGAGGACCAACAGGCCAGGTTGTCATGCCCTCCACTCATGTGCCATGGTCTACAAACAACAACTCCTACACACATATGTGAGTGCATCTTACATCACACACGGAGATTAAAATCAAAACAAAAGAATGAGCTATCTGTTTTATCTGTCAGTATCTTCACCTTACTGGAAGCAACAAGGGCACCAAGCAATTCAGAGACACACAGAACATGATCTTTGACATGACTTAGACCAGTGGTTCTCAACCTGTGGGTCCTGACCCCTTTGGGGGCCCCATATAGGATATTCTGCATATCATATATTTACATTATGATTCATAAAGGTAGCAAAAATTATAGCTATGAATTAGCAACGCAAATAATGTTATGGTTAGGGGTGGTCACCACAACATGAGGAGCTGTGTTAAATGGTCAGAACATTGGGAAGGTTGAGAGCCATTGGCTTAGACAAAGTTGGATTAAGAGAATGATTGAAAGAATATATCGAGGTTTACCTTGGATGCTATGCAGCCTCCCAGAAGTATGGGCTAGATCTTCCTGTGTATTGACTCAGAGAGATGTCTATGACAGGACCTTAGGTGGAAGAGAACAAGTTGCATATGGTTTAGTAAATATATATTTATTTTTATCTTATGTGTATGAGTGTTTGCCTGCATTTATACACATGCACCACATGTGTGCAGTGACACACATTGGATCACCTGGAACTGGAGTTACAGGCTGAGAGCCATCCTGAGTGCTAGAAACTGAGCCAAATGCTTGCTGTTAACTGCCGAGCAATCTCTTCAGTGCCTTTAATATTAATTTTTATGTCTTTTATTTCTACACCTTTATTTCTCCGTGTAAAGAAGAGGGACAGGAATAAAATAAAAATGGCACAATTTACACATACACAGAGACAGACAGACACACAGACACACACCCCCACACAAACATGGTTGGGGTTAGGAAAGGGGGCTGGTTAGTAGACAGGGGAGGGGTCCCCATTACAGCAGCAGGAGCCAGTGAGCTAGGACGCTCCATTTCTCCCAGCATGAGTGGGAACAACCATCTCTTCCTTTCAAGCTACGGTTCTGCCCAACTTGTACTGCCCCGGCCCTTGGGAGGTAGGCAGAGAAGTCTTCCCAGACTACAGAGAGGAGGAACCGAACCCTGCTGTGGGAAAGTCAGACCATGCACAGGGAGTGGCAGTCATTGGCCTCCTCCACCTCCCTAAGGGGGGCAGCTATGTGAGGGCTCCCAATACTTATTCTTCCAGAATCAAGCAAGAGAGAGAAGAGTCAGAGCCTCGTGGGAGAAGTACGTGACAGCCACATCCTGGTCCTGGGTCACCTTCTCCAGGATGGGCCTGACTTCACTCTGCAGGGTTCTGTTGTCAAGAGTGGGTCCTATCTTCTGTAGCCACAAAGCAGACATTGGTGACTGGGTCCCCAGCCATACAAAGAACTGTGGTCAACATGTGTTTGGTGGTAATACCCTGCCCACAGACCTCAGACAGCACATTGATGCAGAAGAGTGTAGTTGTGCGATGTGAATAGTTAGGGTCTTAAAACATGGCTAAGACCTTTGGGACAATAGTGGCATGGGCCCACCCCTTCCCAAACACCTCCACTAGCTTCTTGAGGTTGCTGGTGGCAGCCTCACAGATAGCATAGACATCATCCACTAGCCAGGCCATACACAAAGAGTTGAGTTTCTCGTCAATAAATTCCACCCCGAGCTGTCCAGCCAGAGGCACGAACTCAATGAAGACTAGGCATACTTGCCACTTGGCATCCTCAGCTAGTTCCACAATGTCAGGAAGACTAGCCATACTCGCCACTTGGCATCCTCAGCTAGTTCCACAATGGCAGGAAGCAGAGACTGAGAGAGCTGCGGGATGCCAATCATCTCATTCACGCAATTCAGGTTGGAGATGATGTTCATGCACACCTCAGGACACTCATCCTTCAGCTGAGCCAAGAAAAGGGGCAAAAGGTGTGCGGTGGTGTCTTTACCCAGAATGGAAGAGGAACATGATGACAAAAGCCAGTGCCGACTTGACATGTTGGGTGGCATCTGACACAAGCTCCTTGATGCAGGGCAAGATCTGGGTCATGGTTACATCATCCTGGCAGTTGGCTGGAAGATTTTCACAGAACTTTTTGACCTTACAGTAGGCTGTGGCCCTCACCTTGGCCACATAGTTCTTCATCAGGTTCTGAAAGGCAGGCACTAGATCTGTCAGTGATCTCAGGCCCCACTGCTTTCTGGAGCTCTGTGAACTTGTCAGCTACCATGTATCAGACACTTGTTCCTTAGCACCTGTCTCAAGGTGGGCATCACTAAGGCCTTCAGGTCCTCCTGGGCACAAGCTGAGCAATGTTCACACATGCCTCCACTGCCAGCAGCCTCACCGAGTCCTGCTCATCAGAGGGAGATTAGAGAACACGGGATGCTCTCACTCTTGACACTGTCCAGCTCCAGCACCTTAGTAGACTCCCTCAGCTTGGAGGCAGCAGCCAGCCGCACCATGGGGGTGCCGTCTGAGCACAGGTTCCGGAAGGACTGTGGAAGTTCTGTTCTACCTTCATGGCACTGGATACTCAGGGGTAGCAAACCAAGAAGACACCACGGGCCGAGGTGCAGGAGGTGAACCAGTCTCCACCCACCAGCCATCCGCTTCACCAGAGGCACAAAGTGAGCTTCTAGATCTGAAGGTGAGTGCTCATGTGAGATGGCCCACAAGGAGTCTACCACCTTGTCTCGCACTACTGCCTATTCCACTGTGGCCAGTGACTCAGGGGCTGGCACCCCAAAGTGGTGAAGGCTCCCAGCTACTCAGCCAAGGCCAAGAGGACTGTGTCTTCATCATAGATGATATCTGTAAGGAAAGGCAGGAACTTACTTCTGGTGCATCCAATGACAAGGGCCAAGGCAAAGGTGCAGAGCTTCTTGATACGATTGAGACAAAGCTGAGCGTCGTCATTTCGGAGTTCATCTATGAGCACCACAATGGGATAGAGTGGATCGTCAACATTGGCAGCTGCCATCTTGGCTCCTTGGCATTTCTTTTTAAAGGTACAAACAGTTGGATGGTACCTTTGGAGGGACACCTGAGGTTGTCCTGTGGTCTCACACGTGCACATGTATGCATGTGCACACACATGCCCACGGACACACACATGAAATCAAGAGGTGCATTCAAGATTTGTGTCCGTTTGCTCAAGGCTGAATTCCCAAACCTCGTGTGCTTGGGAACAGGAGAAAAGTGGACACTTGTAACTGTGGCAGGGGACTCCTTATCTCCCTCTCCCCGGATCCCCTACTTCTGCTGACTAAGGCACATCTTCCTGCCTAATCTCCAAAGGAGAACTTTTAGAGACTCGGTGCTGAGCTCACAGAGCCGGCTAATGGCAGGGCTGTGGGGGTGTACAGGAAAGGCAGCTGTCTTTAAAAAGGGACAGCAATCTATCTTTTACTTACATGTTTGTTTGCCCACACACATACTATTCTAGAAGCATGAAATTCAGGAGAAGCTGACAGTAAGACAGATAGGGTGATTGGTTACTGACTGTATATTCATTTTTTTTTCCTTAAATCTCCAAGGGAATTTGGAGCCAGCAACGTCTGCATGTTTGTATCTCCAAGCCGGCCCCACAGGTCAGCTTACATCGGTGTGAGGAACACAGTATTGTATAACACCATACAAAAGTGAGCAGAGACAGGGTTCATGAAATCACAAATCGAAAGTGTTTGCTACTGTTGTTACCTGGTCACCCAAACAGGTGTCCCGTATTTAGTTTAAAAGCCACACAGAAAGACAAAATGGAGCATGTTTGGGGTAGGGATGAAGAGAGTGAGGTATTTGGCATCCTATGTGAGAAGTAGGTTGAGGGAAACATGGGTAAGATCAGATACAATTTGGGTTTTCGGCTGTTTGAGGAGTTGGCTTGTAGAAGAAAGGACTCTGTATCGGATATTGTCTCAGGAATAAGACACAGGAGGCCGCTGTATGGAGGAAACTGCAGGGGCTTCAGCCAGAGAGAGCAAACACCTGCCAGAGATCTTCTGAGTCCCCTTCCCCCATGCTCTCTGTAGAGAGAGTAACACTGTGATACAGAGAAGATCAAACACAGTAGAGCATAAGCCAGCACTTTGCAAATTGTGTTCCCAAACTGCGGCTGCTTTTACTTGGCTCTGTGTGTGTGTGTACGTGCTTATGTATGCATGTGTATAATGTGTATATAATATATTAATAAGTTACATATGTATTAATATAAGACATGTTGTATATATGTGGTGGTTTGAATAAGATTGGACCAGGGAGTGGCACTATTAAGAGGTGTGGTCTTATTGGCGTAGGTGTGACCTTGTTGGAAGAAATGTGTCACTTTGTGGGTGGGCTTTGAGACCCTCTTCCTAGCTGCCTGGAAGAGAGATAGCCTTTTCTCCTGGCTACCTTGGATCAAGATGTAGAACTCTTAGCTCTTTCACCAGTACCATGCCTGCCTAGACACTGCCATGCTTCCTGCCTTGATGATGATGAACTGAACCTCTGAAACTGTAAGCCAGCCCCGATTAAATGTTGTTCTTTATAAGAGTTACCTTGGTCATGGTGTCTCTTCACAGCAATAAAACCCTAAGTAAGACAGTGTATATATTAACTATATATGTATCTAGATGCTGTGACAGATTAAACCAAATATCTCTGATGGGGAGGACTCATCTATTGGTAGTTTTAATATAATTTAATTAAAATGTTTAGACAGAATCTTATATATTTCAGGCTGGCCTCAAACTTGTTATAGAGCTAAAGATGATCTTGGAATCTTGCTTCTCTTGCCTGTGCCTCCTGAGAGCTGGGTTCTAGGCATGTGCCATCACAACTGGAGTATGTGGTTCTGCGAATTGTACCAAGATCCTCATGCACGTTAGGCAAGCCTGCCAGCTAAGCTATGTCCCTGGTCCTCATTGGTAATATTTAGAGCTACCAAGTAATCTTTTGGTTAGCCATTGACTGGTTCCACAGCAAAGCACATATATGAAGTAATAATTGGGTGCTAAGTGATTATAGAAGTAGTCTTGATTTGAAGCAGATCTGTGGGTCAATTTTTTTCAAGGCCCATTAGCTTAACGTTTGGAGATAAAGGCTGTGACTGAGAAGTTTCAAAGTGAAATCCAGAAAGCATAGCCTGTCCACAGCATGAGGAAAGCTAAAGATGGTTTAAGGACCATCATCTTTTCCCAGGCATGTGATAAGTCATTCAGGCTTGAGTAGCCACTAACCTTTCTCCTGGGAAGTCCCAGATGAGAAGGACCAACCGATGAATTGTTTCTAGAGCTCACAGATGCCCAAGAAAAGAGCCTAAGGGGTCTTCCTCTACCTTTGGCAGAGTCAATTATTTATGATTTTATTATCCGTGATTTCTTTATGTGATTGATTCAATGTGCCCTTAAATAATCACTGCCTAAAGACTGGTTCTTAGTGAGGGTCACGGGATTCTGCTTGTGTCCATGTCTGCATTGCTGTGTTGGTAGCTGAAGCTGCCTGGCTGCGTGCTGAGCTCACTGAGCCGGCTAATGGCAGGGCTGTGGAGGTGTACAGGAAAGGACACCAAGTAGACACTCAGATGCAAAGACATTTGGGCAGCCAGAGATGCTGAGCAAAGCTTAAAGGGGGAAAAAATTAGATCCCCACGAAGCTCTGCACTTACCCCAACACTCTTCCCCAAGACCACAGAGCAAAGAAACCAGGCTGAAGCTGTCACAGCTGCACGTGTCCCGTGACTCAGCTCCCAGCTCAAGGCCAATGTGGAGTATGCAGGATAAGGAAGTGGGTGCTCCTACTCTGCTCTACATGGCCAGGTCACTCCTGGCATTGACAAACGCAGAGCTGGGTTTGGGGGGTTTCAAGAGCATCAAGGTCAGAAGGCCAAAGCCTGGAGGAGTCTGCAGATGAAGACAAGCAATGAGCTTCTGGTTCCAGGCCTTCAGGGGGAGCAGATTTGGGAGATATAAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTCTGAGGACAGAATTGGGCTCAGGGCCAGCCCTTTACAGGAACCAGGGAGCTCCGGCAAAGATGGCTTTCCTAAAGAATTAATTGCTTGCAAGGAAAATACCCAGTTCTCAGGTTGGGAACATCCAAATAGAAGTAGGAGAACTCCTGGGGGTTGAGGAGTTGCTGATATACAAGTAGGAAGAGGGTCAACCAGGTCTTATACTGGAAACTCAGGGTGGGAGATGAGGTGGTGGGTAAGGCAGAAGCTCAGGATCTGAGTTGGATCCCTGGTACCCGTCTAAAACGTGGCCTATAGTTATAACCCCAGAGCTGGGAAGGCAGGCACAGGATCTTATGGATTGTTGTACAGCTGGTGAGTTCCATGCCAGTGAGAGAGCCTGCCTAAGGTACGAACAGTTGGAAGGCACCTTTGGAGGAACACCTGAGGTTGTCCTGTGGTCTCACACGTGCACATGTATGCAAGTGCACACACATGCCCACGGGCACACACGTGAATCAAGAGGTGCATTCAAGATTTGTGTCCGTTTGCTCAAGGCTGAGTTCCCAAACCTCGTGTGCTTGGGAACAGGAGAAAAGTGGACACTTGTAACTGTGGCAGGGGACTCCTTATCTCCCTCTCCCCGGATCCCCTACTTCTGCTGACTAAGGCACATCTTCCTGCCTAATCTCCAAAGGAGAACTTTTAGAGACTTTGCTATTGTACCCTGTGCCCATGACAGGCAACAGGGAGGGAACTGCTATCAGAAAAACATTACAAGGACATCGGTGTCCATAGAAAATGTAGCTATTACTGATAATTCTTTCTTTTTATTTTTCTTTATAAAGAAAACAAAAAGAAGATTTGGGGGTGGGGTGGGGGGAACATTCCTCCAAGTTTCTGTTGACTCTGTTTTTCTGGTTAGTTATCTTCCCAGACTTGGCATTGAATCAGGTTAGGCTAAAGAAAGCCTTAGTGAGGACTCATTTCAGATTCATCTTTCCCAGACGGAAGAAACTAAGGCCCAAGGGCAGAGTGGTTTGTCTAAAGGCACTGGGTGCCTCAGGGAACAGCTGGGACTGTAGCCAGCCTTAGCCCAGTTCTGACCATTCCTAGACATTTCCTGTAGGCTGCCTTTTGGGAAAGTACCAAATCACCATAGTCTCTGGGCCTTGAGCAAATGGAAATCTGGCCCTATGGCTGCTCCTTTAAAGATCTGAACGGGAAACAGACCTCGACCACACATTTCTCCTTCAAGTCTGTTTCTTTTACATAGTTTTCAAGGCCTGCCACCCAAACTGTGTTACCTACATTGGTTCCTCGACAGCTTGAGGGGGATGCCAGGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGGTTTTTCGAGACAGGGCTTCTCTGTGTAGCTCTGGCTGTCCTGGAACTTAACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTTGATACACGGATGTTCCAGGGTTCAGAGTGGGAAGCTCAGCTTCTCCCCCGAGAAGTAGCATGGTGGGGGCTTCTAGGTTGTCCCTTCTTCCCAGAGATGCCCATAACAGGTGTCCCTGTTCTCAGCAAAAAGCAGCTAGGGTGCCTCTCAGCACCTCTGGTCACAGACCCAGCTACTCAGCAGCAGGGCTCCACGGAGAACTTCCACCATAGGCTCCTTGTTCAGAAACCAGTGTGAGTTCCAAGTCTGGGACAAAGTAGTTTTTGTTTAAAAAATTGAATTAGTATATATTAATTATACATAATGAGTTTAAGAATGGCATAGTCATATATATATATTCCCCTCCTTCATTTCTCTTTCGTGTCCTCTTTGTCCTCTTCTTCCTCCCATTATCTCTTCTCTCTCTCTTCCTCTCATAAATTTCATTAGTGTTGCCCACAGGAGCATGCGGTGAGCAGTTATTTACAGAAACATAGACACCTTACCATTGGCTCTATCACCTAAGAAAATGGGGTAGTATACTTGAAAACAGGTGTGAGGGTCCTAGGTCCAGAGGCAGTGAAGCCCACCCTGCTTGACTGGGTCCCAAGTGCGTGGGAGCCATGCTTAGGCTTTACGAATTCAGGGACAGCAGAGGCCTTCGGGATTACTGATGTATCATTGTCTCATTCTTAACCCGCTTCTGTTTCCTCCATCTGTCTGCCAAGGGACCAGCAAGCAGCCCAGTGCAGCGTTTAGGGAGCCAAGTACTTCGTGTATACTCTTCCCTAGCACAATGCCTACGCCATCTTCTATGTTTCTGTTACTCTCCCATTAAAATGGGAGCAAAGAAAAGGAGTGAACGATGTGGAAGGATGTGGAAGATGAACATGCAATAAGGTGGGCGTCGGGCGGTGGAATCCTGTGCCAGTTCTGGGCTGGAAGCAGGCCAGTGTAGTGTGTGGATTCCTTGTCCTCTAAGACTCTTCTGTTGGGGCCTGATTGAGAGGTTTTTGGTAGCTCTCAGGGATTAGAGCCACCAATTTAGGGGCCCTACAGCAACCAATGCGGTCATTTGTGATGCCCATGCGCCCCCTGGTGCCCCCCGCTGGGTGCTGCGCCTCTTACCCCCACCTCTAGGCTTCCAGAAAGCTCCTGGTGCATTCTCCGGCATTCTCTGGGCGTGAGTCACGCAGGTTTGCAGCCAGCCCCAAAGGGGGTGTACTTGAGCAGAGCGCTATAAATAGGGCGCTTCCCCGGTGCTCACCACCCGCGTCACCAGGAGGAGCGCACTGGAGCCAAGCCGCAGACCGGTGAGACAGCTGCACCTTTCCTACCCCAGCGCCGCTGAAGCCACACCGGCAGGGATGGGAGGGCAAGGGTAGCAGCTAGGTGGAAAGCTGGCGCAGCCGTGGGGAAGTGGGAGGTGACTAGAGCCCCATCACCAGACAGCCGAGCATGGGGTTGGGTAGGGGGTGCACGCTGGCTCAGACACGGGAGTCTCCGTGTTCTTTAATCCGGATCACAATTTCACAGCTGTTCAATAGGCCACATACAAAGATCTTCCTTTTGCAAAAATGGGGAAACTGAGGCTTGAAGCCTGCCGTACGATGCGGGAGGCGCAGGGGTGCGAGTGGCTCAGCGCCGCGACTAGGTGCGGATCTCTGTAGTGGCTGTTGTCTTTTGGGCATGAGTCATTGCATTTGGACCTCCCTTTCAGTTCCTGGGACAGCGCATAATAAATAAGATGTTGTGTATTTAAAGACGTGCTGCAGACATCCAGACCCGTTTTGCTGAGCTGACTGCATTCATCCAGTTCTTTAGAGAAATGCTCCACCAGGCCGAGACGTCTGATCCCACCGGATCACTTTATAGATGGTAATCTGAGGCCTGGAGACCATTAGAGGCTCAGCCTCCAAACGTTATATGAATAAGAAACTGAAGCCCTTTGGCTTTTCCAGAATTTCTGGGCCCAGTTTTAAAAATGGTGCTGGTGCTGTTTTTAAAGGAAGAAGTTAGGAATTCAAGAGTCTATACAAGTTCTCTTAACAGGATCATACAATTTAGTGCCCGACCCTGTGAGAATAGTAAAACAAACTGCAAATGGTAGTCAATCAATGGAATATTAGGCAGCAGCAAATGTGTGACTTTTAAAAATATTTAAAAACAGGACCTATGAAGGGATGGTTTATAAAAATGCATTGGAAAAAAATGGAGAAAATACGCCACAATGGGAGAGGCTATGTTTTCCTACTAGATAAGGAGGTCCTTGGCCCTGGTGGGGCTGATGCACCCAGGGTAGTTGGCAGCCAGGCAGTAGGCCAGCCTCCAAAAGAAGACAGGAAGTAAGTCAGTGTAGAGGGAAGGCAGCGGGCTGAACTGAGCAGCGTTGTCCTTGGAACAACAACACCACAGCGCCGGGAAACTTGGGAGACTAGCAGATTCTGACCCGGAGTCCATATTACCAGCAGACCTAGAGGGAGTCTGTTCTTAGCTTTACTGAATAGAGGATCGCTTCTTTGAGGTCTGTATCAGGTCCATTTTACAGATAAAGAAACCAAGGCCGAGAGATTTAGGACTTTTTATTGTCAAGGAAAGAATATTAAAATGAGGTTTCAGAGGGCTGCATTCTTCCTGGGGGGTGGAGAGTGGGGGTGGGGGTTGGCTACTGGCTTGCAGTGTGTCTTGGGGAGCCTGGCAGGTGTCTGGGCGTTGGCATTCTCTAGAAGGGCCCAGGCTGAAGAGCAAGCAACCCTCCCTTTCTTATTTGGGAACTTGGTGGCTCTCCAGACCCCAGGTCTCCTTTGGCAAGCCCCAAGCTCCCTGCTCCCTGTAGCTCATCTTGATACTGCTTCGCCTCCCATTCAATGGTCTTCCTTGGCTGCCCTGCCCTGCTGCCTCTTCCTGCCTCGGATCAGAAGCCATTTCCAAGGCTGTTAAATGGCTTTTCCCAAAATGGTCATGCTTGAGGCTACTCCCACCTACAGATATTTCTGTGGCTTTGTCTCTGATGTGTCAAGGGGTCTGTCCCTGCCTGGAGCATTTCAGGCCTCAGTGAGCACCTGGGAGGTCTATGAGATCAGGGCCTTCCTAGTGGATCATGAACCAGGCTGGCTGAGCCACCTACCTATCCCTGGGAAAGTCTCTTCTCCCACACCACTCCTCCTCTGCTCTGCGCGCGTAATTAAGCCCTCAGCAGAGGCCTTGCCTATTAATGATCTGCCCAGTGTGTTGATGGGCCTGCCTAGCTGAGAGCAGGTTTTAAGAGTGAGCCATTCCAGGAAGAGAGATCGCTCCCTTTTCCCTGTTCCTGTGGGTATAGGAAGTTGGTGAGCCCAGATAAGCTATTCTGGGCAGCGTTGGAGATGCAAGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTATGCAACCAATCCAGCTTCTGGGAAAGGATCCATTGTGTAATACTGGGGCTTCAGCCTGTGTCCCCTGGCTCAGGGCAGCCGGCCCAGTGGTGGCAGTGGCCCCTTGGCAGAGTATGCTAGTTCAGGCTTAGAGGCAACAGGCATGTGAAAGGAATTTGCTGGAGCCAATGGGCTTGTCCTCCTGAAAGGGCTTTCTGGGGCGGGGGGTGGGGGTGGGGATGGGGGCTCACTGCTAATCTCAGGCTTTGTCCTTCCCCTTTCTAGTTCTGCCTTCTCTGCAGTCCTAAATTCCTTCCCTTCCCATTTTGCTTTTACCCCACCCCCTCCCCAATCCATACTCAGCCTGGTGGCACAAGCGTTGCCCAGAGATGGTGAGATGGATTGAATTTCCCGTGCTGATGGAAGGGTGTGCATATTACCACAGGCTCACTTGGATAGCCTTGGGAGGCTGAGGGCCCCCTAAGGGGAGCCCAGGGCTGTGCAGTGAGTGGGGATGCAGTACCATGGAAGGGGATAGGCGAGGGATGTTTGCATGTTTCGGGAGAGGTATCTTTAGTATTTGGCATGTTAAATATGCTCTGGAGACACAGGAAATTCATCTGCCCTGCCTCAGGCTCTTATGGGAAGAAAGGGACTCAGCTTGGAGCTCGGCCCCTCCAACTCCAGATAGCCTGGCTCCTCTGTGCAGCCGGGCCATGGTTCTTCCCAGGGCCTCGCTGTCTCCAGTGTCTATAGAAGGGGTCAGTTTAATCTTGGCTTTCTTCCCCAGTTCTTGAGAAGTAGATTGCAGTGATGCCAGATGAGGAGACAGATGAGGGCCGCGTGTTCCAATCCCACCCCACCTCCCCTCTCAGGGAGCTGTAGCAGGGTGCAGAAAACCCTGAAGCTGCCCAGGGGTTGCCATGACACATCTGCTGTTGTTTCCAGGACTCCAGATTCCAAGGAGGCCACGCCATGAAGATTCTCCTGCTGTGCGTGGCACTGCTGCTGATCTGGGACAATGGCATGGTCCTGGGAGAGCAGGAGGTCTCTGACAATGAGCTCCAAGGTAGGTAGGCTGAGACTGTTCCTCTGGTGGATGCTATAGGGCCCAGGGCAGGGCCATTCCTCAAAGCTCAGTCCCCAAGTGCTGAGGGCTGTTTTCATTCTACATCCTGGGTCTGTCCATGCCCTCTGCCCTCTCTAGGTCCTGTCTTGATGTTTTTGTGGTCAGGTGACTTCTAGACATTGCCCTCTGTCTCAAGCCCCTCTGTTGGCTGCCTGTCATTCCTTTGTCATTACTGGTCCCCTCACCTGGCTCTCTTACTCTTCTCCCAAAGCCGTGCCCTAGACCCTTTGAAACTTTGTCAGTCTTCCCTTTGTGTGACATCCAGGGTTCTTACCGATGTGTCTCCACACCAACCCCTCCTGGAAAGCCATATCCACCAATTTCCTCCTCTGGAAAAAGGGCAATGCAATTTGACATGAAGTAGTCACCTGAGAGCACCCTCTCCCTTCCTCCCCATCTTCCAAGGCTTTTCTGGAAACCCACCTTTTCTCCCTTCTTCTCTCCTAACCTCTTGCTCATAGCTGACCAGTTCTGGGGGGGGGGGTCTTCGTTAAAGACAATGTGATCAGATTCTTTGATGTGGATACACACGGTGAAATGATTGCTGTTGTCAAGCTATTAGCTGTCCATCACCTCAACTTATCATTGGGTTCAGACACTTAAAATATGTTCTCTCAGCACACTTCAAGGGTAGAACACATTATCAACCACGAGCTCTGCTTGGACCATTGACCTTCGGGCTTACTTGTGTTGCTACATCATTGAAGCTCTTGCCCTTGGCTCAGCAGTCCCCCCTCCCGCTTCTCTTTGGATGTGTCTGGCAACCGCATCAGGTAGAAAACAGCAGAGGTGTGGGAAATGGCACTTGACACAGGATAAGTAAGTGGTACCACCGTGACATGATGTGTCATTGCCTTCGTCACCTGGGGCCGTGTCATGGCTATGGTCTCCATGGTGAATGCAGCTCTTCATTTATCGTCCCGTCTCCATGGTGTCTGGTGCTAT
D3_15 64927807 64942857 15051 CTAGATTCATTTCAATAGGTTTTTCTAACCCTTGCTGTGGGTAGGGCATGGTGCAGGACTCAGCGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATATGAGCATCCAGACATTTCCCATTCTTTTCTTCTGTATTCAAAATGACTTTTAACATGAAAGTTCTTATTGAAATATTTTAAAATCAATGTAAAATTCTTGTAATCTATCCTTAGACATCCATCCATTCATTCATTCCCACTCAGATATAGGGGAGGGGGGCAGTTTTCAAAACCCTTAGTAGAAGCTGCAGTTGTGGCTAGCACCAGGCCCTGCAGAGATTGTGCTTCTGTAATTTATAAATTACAGACAGTAAGGTATGAGCAGCCACAATAAAATAGAGCAATTATAATGCTATACCAGTTATACCATACAGAAGGCTACGCAGATGTGATTTCTCCCACACAAAATATCTCAATTCCTCAGCTTCCACAATACTATTTTTAGACTGGTTGACCATGGATAGCTAAGAACTGATAACAAGGGACTGCCCTGTTACCATTTGTTTAATTTGTCATCTACATTTGAACATCAATGTTGACTCTGGTACACACACACACACACACACACACACACACACACACACACACACACAAACTTATCCACAGATTCATCCATCCACTTAATTATTTTCCATATTGATATGTAGACACACACATTTGCTATAAACTAGTATCTCTTCAGGCGGAGAATTTAAATTTTTCATTTTAGCAATCCCCAGAGCCAATGACTGGGTCAGGGGCATGGTAATGAGTTTGATGGTTGATTTGACTGGATAGCTTCGATTTGCATGTCAGAGGCCACAGTTCTAACAGAAAGATAAGACTGTCCTCTTTGGGGAGATGAGAGGAGACAGTGGGGTGGTTTAAAGGAGAGGTGGGGCCTAAGGAAGAGACGGTCAGAGCAGCAGATCAGAGTGGACAGGGAAGCTTAGGGAATGGCTGGGCCTTGAAGAAGGAGAGGCTCCTGAGAGCGTGGCTGGGGAGGCCCAACAGAAGGGCTAGAGCGAAGCTGTGGGCACCAGGAGCAACCTTGTACTCAGATCGGGGAGACCGGCTGGGTCTCTGTGTGGGTCACCTGGCCTTCTTGTCATCTCCCTGAAGGATGCTCTGGAGGACACTAGGGATTCTGAAATGAAGCTGAAGGCATTCCCGGAAGTGTGTAACGAGACCATGATGGCCCTCTGGGAGGAGTGCAAGCCCTGCCTGAAGCATACCTGCATGAAGTTCTATGCACGTGTCTGCAGGAGCGGCTCAGGGCTGGTCGGCCAGCAGGTGAGAAGGAGAGATGTCCACAGCCTGGGTTCTGGATGGGGAATGTGGAGAATCCATGAAATAGGCCTCATTCCATATGCTGGGTACAGTTGATTAGCATTGCTTGACTGCACATCGTTCTAGTGTCATGGTCGATTGGTGATGTCTGCTTGGCAGGTACCATCTCTTTCAAGACCTTTAACTGGTTGGGTAAAATGGCCTTATGGGATGGTAGAATCCTGTGTTAGAATAGGAAAAGGGAATTAAAAAATATTTAGTCTATTTGCATTAATTCTATCCCTACCTGTGAGATTCACTTGGTTGGAACTCAGTAAAATTCACTGTTTCCTCAAGGCTGAGCAGCTAAGCAGGAGTGGCTATTTATTCTTTATGGCCCAAGGTGAGACTTGGGAACCTCCGAGTTTCGCCATGAGAAATGGATTTGGGGGGCGGCGGCGGGGGGACGGGGGGTGCTGACAGGGTACCCAGGAGGCAGAACAGTGGGAACTTGGGTCAGTTTCTGTGTGGCCACAGGGGGATACAGGCTGGAGTACACACAATGCCTTACTCTACTTCTCTGGGTCCCAGCTAGAGGAGTTTCTAAACCAGAGCTCACCCTTCTACTTCTGGATGAACGGCGACCGCATCGACTCCCTGCTGGAGAGTGACCGGCAGCAGAGCCAAGTCCTGGATGCCATGCAGGACAGCTTTGCTCGGGCATCTGGCATCATAGACACGCTCTTTCAGGACCGGTTCTTCGCCCGTGAGCTTCATGACCCCCACTACTTCTCCCCCATCGGCTTCCCACACAAGAGGCCTCATTTCTTATATCCCAAGTCCCGCTTGGTCCGCAGCCTCATGTCTCCCTCCCACTATGGGCCTCCGAGCTTCCACAACATGTTCCAGCCTTTCTTTGAGATGATCCACCAGGCTCAACAGGCCATGGATGTCCAGCTCCACAGCCCAGCCTTCCAGTTCCCAGACGTGGATTTCTTAAGAGGTTAGAAAGCAATTGCTTTCTTTCTAAGTAGGAATGTGAATGTCCCAGATGTCTAGGCTCTTCCAGGGCTTGACCTGTGCTGGTTTTATGTTCCTAGAAGCCTCAGAGGCTGGTATTTCCTCCATTTGTAGGGTAAAGGAGCTAGGATGGGGGCAAAGAAATGCAGGTAAATGTGGGGGGGCGCAGTTCAATTAGCATTTGTAGAGATTGGAGCTCTTGTGTTTGTTACAGTGAACTCAGGGTGTGATCTGGGGCTGTGCCTCCTATAGGATCCCAGATATTATTTCTTGGGAACTTTTGAGGGTGTGGTGTGAGAGGCCCGCCCTTCCAGGCAATGAGTCTGTGTCATCTTAAAGAAGATGTTCATGGCTGTGACCTTAGCGTGTAATGCCTGTAAGGATGAAATATCACGTGGACACGAGAAACCGGTGTCTGAGGAGTGATTTTTTTTTTCCATTAGTGCCTTGGGTCTTAATACCATGTCCTACATGGTATGGTTTGCATTAGAAATAGAAGAAGTCTTGGCATCCAACACACATCTACTGTAGCTCCATTGTCTAAAGATGGTTTATGTTGTCAGGACACACTTGTGCTCTCTGGGGCTTGAAGGCCAGTTAGGGAGTACCATCAGTCAAGGAGAACAAAGAACAGCCATGATGTCAGAAGGCACACAGCTGAGGGGATGTGGCAGGAGAGATCTCCTCAGATGGAGATGGTGGGAAGGCAGGCAGGACCTGCTGAGAGCCGTGGGAATATCCTCAGCCACAGGGAAGTCCCATGGCTGCAAGCAAACAAATAAGCTGCATCGTGCCCTGACCATGGCACTGAGCAGAGGTGTGGAAGGCTGTCGGGTGGGCATCCTTCTGATGTGCCATGTGTCTAATCCTGCCCCTCTTTCCCCTCATGTGTCCAGAAGGTGAAGATGACCGCACTGTGTGCAAGGAGATCCGCCGCAACTCCACAGGATGCCTGAAGATGAAGGGCCAGTGTGAAAAGTGCCAGGAGATCTTGTCTGTGGGTGAGTCCGGGAACCAGTGGTCATCTGACTGTGGGTCTCCAGTGACTCACTGGCACTATCCTTTTCCAAAGACTAGCTCTCTGCCTGGGAACTCTTAATCCCCGGGAGACTCCTCACCCTGGTGCACAGCCTACTCTCCTCTTATCTAGAGCTTCATTATTTGGGAGCTGGGGTAGGACCCAGTGCCTCCTGCATGTTAGGTGAACGCTCTACCACTGAGCCACATCAATTTTACTCAGAATCAAGTTCAGGTGCAGATTGTTCTCATATAGATGAGGTGGAGCTAAAGTGCCCACCACCACCCCTGTGGCTCACTGGCAAACCTGCAGTCCACGGTGGAATTACACTGTATCGACCAGTAAGCCACAGCTCGTTCCCCTGGCTACTTTGTTCCACAGTGCCAAGTACTGTAGACCCTGATTTTGAACTTCTGATTATGAAATCTTTGACAACATGCGTTTTGTTTAACAAGTGCTCGCCACATGCCAGGCACTGCTGCAAACACGTCACAGGTGTCTGTTCGCAGAATGATCCTGTGAGCTGGGTACAGCTCTCTTTCCGTCTTGCTGGATGGCTAAGTGACTTATCTAAGATTACGTGACACGAGGCTTCTCACTTAGGCTGTGCTCATAAACAGCACCCTGGGGTTTCTCCCTGGGGACCTTGAGCTATGTCCTTGTGATGGTGGTCCTTGGTATCCTGGCCGCATTCTTTTTGGTGAACTAGTCCCTCAGAAAAGGAACTATTTTTCCTTCATCAGTTCCCCAGGCAATCTGGGTATGGTGACATATCCCTATAATCCCAACATTTGGGAGGCTGAGGTAGGAGGGTGGTGAATCCGAGGCCAGCGGCGAGGTTCTGTCTCAATGAAAACAAAACAAAATACAGCAAACCCAAAACTGTTCCCCACAGATTTGTCAGGTCTGGCTATCAAGCCAATGCCCCGCCTTCAGCCTGGTCTTTACAGTGGCTCACAGTACACGGTGCTACCTGCTCAGATCACCCTCTTTGTTGGCCGGGACAGACCTTTAGAGTCTGGTTAAATATTCCCAGCCTCATTCCTGCAATGTTAGGAGGCTAGGAAAAAAAAAAAAATACTAGTGCTTTCAAAGGTCATAGTGTGACATTATAAACAAATATGGGCCACACCTGTAGGCTTTCCCTTATAGTTGAAAGATGGGGTGCCTGAGCACAGAGAAGCCAGTCACCTTATAGACTGGATCTCTTACATCAGACTTAGCGATCCTCTCAACAGTCACCCGTTAGCTCTTATTTACGGTGCTTATAATGGTATCCTCTCAGCAGTCACCGTTTCTGTCACCCGTTAGCTCTTATGTACAGTGCTTACAATGGTATGGATAGGCTTCACAGAGACCTCTCGCCTGGGACATCTTGCCTCAGCCCTGTGGGCAGACCTGACCATGGCTGAGCATTTAACAACATTTTTGGCTCCAACCTACCATCTACCAAGAGCAACCCTCCCTCCACTGTGACAATCAGAAACATCTTCAGATTGTCTGGACTGTCTCCTGGTGGGTGGTGTGGTCCCAGGTTGAGAACCACCGTTCTACTCTGTCCTTCTCTCAGGACTTTCTTGTTTGGGCTAATCAGTGGTTAGAGTGCTCCGGTTATTCCTGGAAGATTTCTTCTCATTTCTGCTTCTGGTCTCCAATCCTGTGTCTTGCCCCATGCAACATTATATCTTATTTTAAGTATGAACAATTGTAGTAGCCACATAGGGCCCAGAAGGCATCTCTCTTGTCTTGCTTAGTTTGGATATAGCCTCTCATAGATGTTTAAAAAATAATTGAGGAGGGGGGAGGCGTTGTTAAACACTGGTTTTCAGAAAGCATGAGCACTTTGTTGGTCTCAGTAGCCTGATGCCTGCAGTGCACTCTTGCAGAAGAGAGGATGCCAGAGTTCCTATTTCCTCCTGGTCATCCTGGGTGGACAAGGGCAGGCAAGGACTGACCCTTGGGCACCAAACACCTGTGTTTTGTAGACTCGGGACCTGGCTGTCTCTTCTGCAGGTTTGGTTCTGGGAAAATGGTCTTGCCGAATCTGTCAAATTATGTTCAAAAATAAGTTTGGAGAATATTCTAGAACCCTGGTCTCAGGGGCTCAGGTTGAGGAGTGAAAGATGGGGCTTCAATCCTGGTTCCACAGATCGCTTCCTCTATAGATCTGAGCAAGGTCCACCTAAGTAAATAATGATCCTGTTCTTTGTGGGGTGTCCAGTGCCACAGTAGCTTCCTCCTATTTTGGAAATGAGTCCTGGGAGGAGCTCAGGGAGCAAGAGTAAGTCCAGGAACATGGGGTAGTCAGCATAGGTGCTCCCCAGAGCACCGTCAGCAGCATTGTGACAGGTCACCAAGACTGGTCATGTCTACCGCCCAAAGAAGACTCAGCCACGCTGCTCTGACACAGTTTAGCACAGCTAAGCCATTGTATCCCAAAGCACGAAGGGGGCAGCACCTTGGTTTCCGTGTCCGGGGAACTGTAAGAGCTGATCTGTAGCCCTCCCTGGAAGAGTTCCTCTGCTTCAGATTGTGGCTGCTGCAGCTGGTTCCCAGGGGCGTGTCCGTCCCCTTTACCCTCCCTTTCATGCTTCTGCCTTCTGCTTCTTTCTTCTCTGGGCAGACTGTTCAACCAACAATCCTGCCCAGGCTAACCTGCGCCAGGAGCTGAACGACTCGCTCCAGGTGGCCGAGAGGCTGACAGAGCAGTACAAGGAGCTGCTGCAGTCCTTCCAGTCGAAGATGCTCAACACCTCATCCCTGCTGGAGCAGCTGAACGACCAGTTCAACTGGGTGTCCCAGCTGGCTAACCTCACACAGGGCGAAGACAAGTACTACCTTCGGGTCTCCACCGTGAGTTGTATCCCAGTCATTTGATGAGGCTGAGACCCGGGTGTGATCCAGAGGTGATGTGTGTCTCTTAGAGATGTGGTGGGACAAAGGTCCCACCTAGGATTTACACCAGCAGGGACTAGGAAAACCGTATGCACGTTGTAGCATTGAAATGCCTTTGCCCCGTCAAATATCCAAGTCTCATTGTCAAGATGAGGAAAGAGAGAGGTTCTGTGGTTTGCTAAGAACAGACAGTTTTGGGCAACTTCCCTGGGTCAGCCTGTCAGCCTGGCTGTAAAGCTGCTTCCTGCCCAGGGCCACTCTGAGTGCCACATGAATTGGTTCACTGTTAGCCAGTGAGAACTCGGTTTCCTTTAGTTTGTGGACCCCACCCCTACCCCCTTTGACCACAGACTGGCTAACATTTGTACAAAGCAAATGTTGGGGCCAGGTGTTGTGGTGCGTGCCTGTAAGCCAGCACTGAAGAAACTGAGGCAGGAGAGTAGAGAGCTTGAGCCTGGGCTGGACTATATAGTGACAGCCTCTCTCAAACAAAGCAAAACAAGAGAACACAACAAGAAGAAAACATTCAAAAATCATTTCAAGTTTCTGGAGTGCCTACTTGGCTAGTCATTTTTAAGTAACATTAAAGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAAACAAACAAAGAAGCAAGCAAGCAAACAAACACACAAACAAAAAAACCGAGATAGTTTTGCTCGGAATTCTCTAGGCATCCAGAAATGACAGATAACCCGCAGATCCATATCTGGCTGAAGGGGGCAAAGGGCAGTTCCATATCCTTTGACAGAGAAGGGTGCACTCTGCAGCCATCCCGTAGAGACACTTCTCCTGCCTTCCTCTAGGTGACCACCCATTCCTCTGACTCAGAGGTCCCCTCCCGTGTCACTGAGGTGGTGGTGAAGCTGTTTGACTCTGACCCCATCACAGTGGTGTTACCAGAAGAAGTCTCTAAGGATAACCCTAAGTTTATGGACACAGTGGCGGAGAAGGCGCTACAGGAATACCGCAGGAAAAGCCGGTAAGCGGGCAGCCTTTCTGTGACCCCAGGGACTGGTAAACAAGACCCTGGAGGCCTGGAAAGTCCTCAGGAGTCCAGACAAGGAACAGACTATGCATTCTGGGCACGTGGCCTATCCCTGAGGCCAGAGCCCACGGGGAAGCTAGGAAAACCCCGGGGATTTTACTTTCTTTGCTCTGGGAAATGTTTTTCCTTGATGGTTAAGGATGTGGGTGTTGGCGGCTGCTTCTTGGGGGATACTGATTCTCTTATCCACTCTCCTCTTTTTTTTTTTCAAGTGCGGAATGAGATAGAAGCATCACCTTCCTATATGTAGGAGTGTCTGGGAGGGAATCTCCCAGCTCCCCGAGGTGGCTGCAGACCCCTAGAGAACTCCACATGTCTCCAGGCGAGTAGGCCTCACCCAAGCAGCCTGCCCTTCCTCTGGATTCTGTACACTAATGCCTGCACTTGCTGCTCTCGGGAAGAACTGATTCCCCCACGCAACTAATCCAATAAAAGCCGCCTTGCGATACGTGTCCCTTGGGTCTGTTTTTTATTTAATCCTCCTGCAGGCTGCACATCTTGGTTTGGTATGGAGGGGAAACAAGGATAATAGCCGGGTCAGTTGCTAAAGCACTTGCCACACGAACATGAGTTTGGAAAAGTCAGGCATGGTGGCACCCTCTTGTAATTTAACCTCGGCGCTATCAGAACAGAGGAAAGAGGAACTTGCTGGTGTTCGGTGAGCCTCAGGTTCCAGTGAGAGATCTTGTCTCAGAACACATGGGTAGAAAGAGACCAAAGAGCGACACGAGGTCATGCATATAAAAATGAACAGCTGAGACTATATAGGGGTCTCCATACACTGTAGAGTACTCATGACAATTGGGGCACCTTCAGCTTTGAGAGAGGTATGTTCCAATGAGCCACGGAGGTGAATTCCTGAAGTCAGAGACTTTCCCTTCCCGTTACAACACTGTCTGTGAGTTCTGACAAGGTGGAGCGGTCTCCCAGAGGAGGCACTGACATCGAGGCAAAGGAGGCTGCTAGTGGGACAAGTGGGTGTTTTACTGTGTAACTGACAATTCCCTGAGGAAACTGATCAGCAGGAAGCCACATGAAGATTTTATTTGTAAGAACTCGGAAACCTGGGGCACCTTCTGAATCATGGCATCTCACATCACCGTCCTTCCATACAGTCTATACCCCTTTATTGGTTGTTTATCTCTCTGCTTTCATGGTTTTTAGTTCACTGCTTTTTAGTTCACTGCTCACCCTGAACTGTTAAGTTAGCTGCAGTACCAGGTGCTGCCAGCAGGTGACGCTGTCCAGCCATAGTCTACATCCCTATGGCGCACATCTAGTGTGTTATCTGCAGAAGTTGAGAGAAGCCATAGTGAATGACAGAGGGCATTTAGTTATTAGTTCTGAAGCTAGTAAATATCAGATGAAGAAACCCAAGGAATTCACTGTGGCCTTATAGCTACCAAGGCTTCTAGCGCAGTGTCCTCTCCAGACCTAGTCTTTCCTGGTGTTTTCTAGAGGAGCTTGGTCAATTGAGTATGCACAAGGAAAATGAAGAGACAGTGTAGGACCAGTGAGCATGGAGCCAGTCTGCGAGATAACAAATGTTGTTACACTGTGACGAGAAAGCTCACGAGGTTCAGAACGAGCTGTTTGCCTCTGGGCTGTTTCCAGTTTCACCTCTGATGCTAATATCCCTATTGTGGTGTGCCACACTGACTACGCCATTACTCCATGACGGCCAGGCAGCATCAGTGTTTGCACAGTTCTTGCCCAGGCTCAGTGACTTGCAGTCCAGCAGAAGCTCTGTCTGTCTCTGAGCCAAGTGGAAGCAGAGGAGGACTCGTCTAGGCTTGGAATGGAGTTGCTGTGGATTGGGACACCCTGATCTGAACTGCCCTCGCTTGTACAGGCACTCAAGAGAACAAAGATGGTTGTTAGTTGGTACCAGGCACCTCTGTGACTCCACCTTTATCTTTCTGGGTATCCTAGGGCAAATCACAGGTCGGGGGTGAAGGAAGGACCACTTTTCCCAGCTCACGTTCACAAGGTTCACAATCTTTGTGGATGATGTCGTATGTGTAAGGCCACAGCAAACAACAGGCACTAAAGATAGAAGGAGTTAGTTCTTTGAAACTTGAAGGCCTTCAACATAACTTGCTACTGCCTTTCTCTGGCTATGTAATATCTATAAACATATCTATAAACATATGTCAAGAACACGAAAACAACAACCACATGGGTGACTCCTTTTTGGAATTTTCTGGAAATGTCTGGGCTTTTCTTAGTCTTTGAAAGGCCCTGTCTCATAGAGAAGACATCAAGGCAAAGCTGACATCATCAGGTTTTAGTGACCTCTGTTCCCCATTGCGTGGTCACGTTACACCTCTTTGTGGATTCCCATTCTAAGACCCCCTTGGCTTATGTGATTAGGCTCTTTGGAATTATATTTCTGGCTCCCAATCTCCCATTCTTATGATAGAATCTGTCTTAGGCAAAACCACAGGCAGGGAGCTTACAAGGACCAGAGGACCTCACTTGTAAATCTGAAGCCAGCCATGGTATGTGGAGCTCAGTGTTTGCTCTCTTCTCTGAGACATTCATGGTCAGGTTTTCGTTTTTTGTTTGTTTGTTTGAGCCATGGCAGGCACACACATTAGGTTTAGATTTTCTCCTGTGTACAGCTTGTCTCCCTCCTGGAGACAGCTTGAGACTCAGTCCAGAGATCTGCAAGAGGCTACCATAGAGGCTACCATGGGATGCCTAGAGAGATCCCGTCCCCTGAGCTCTGTGTTCTTTCCCTCTGTTAGTGAATTTCAGTGACTTTCGCCAGTCACCTCTACCCATCTGCCTTCCTGGCAGATCACATACAATGTCGTAGTGAAGCCTCTCAGGTGACAGTTTATCCATTCTCTAGTTTTGCTTACTTCCTATTGCTGGGACAAAAAGACCCCAATAAAAGCAACTGATGAGAGAAAGTGCTTATTCTGGCTGGTAGTACAGACACAACCCAGAGGGCTTCATCTCCTGATAGTTCTATAACCTCTCTCAAACATCTCCACCACTTGGAGATCAAATGGTCAGGCATGTGAGCCTGTGGGGACATGCACATTTGGGCCACCACACAGGTGGCCTGGTCAACTTGTCTAAGTTTGTCCTATCTAGAGCCAGAGACTCCGGGGCACAAGGACCATTTGTCATCAGCCTCTAAGTGTCTCCTGTATTCCGGTTTGGCAAGAACCTCAGAGTGATGAGCAGGTGACTGCATCCCACTGGAGCCTCAGAGCTCACCTGGCCCCTCCTTTTCTCGTCTGCATACCTGAGGAGTATGGGGAGCAGTGGGCATCTGGAGAGAAGTGGAGTTGGTCGCCTCTCACCCAGATTCAGCCTGGAGGACTGATGCTGTACCTCCCACCCCGCCCCATCCAAGGCACAGCACACACTGGCATCCATCTGTTAACTCGATTTTCAATCCTTGCATGTTCTGATGATCACTGTCCACTTTCTTAGAGTCCAGGCTTGGCAGACAGAGACACACCTCAATACCCATGAGTTTCCACAGCCAGAGAGGAAGCTAGTGTTTGCCACCAGGGGACAGAAGAGCTGGCTCCGCAGGCTTTGGCTTCAGCTGACTGGGAAGCTGCTGACAAGATGGAGCTCAGCCAATTCGGGAACATGGGACAGAACTAACAGGAGAGAAATCGGGGGGGGGGGCTCAGCACACATCACTCAGGGTCACAAGGGTGGCACATGAGAGTCGGCAGGATGGGTGCCATACTCCTCAGGTATGCAGACGAGAAAAAGAGGGGCCAGGTGAGCTCTGAGGCTCCTGTAGGATGCAGTCACCTGCTCAGTTGTCAGTTGTTCAGTTGTCAAGACACCAGCTCAGAGAGAGAATGAATCACACCCATTGGAACTCCTAAGGATGCACAGGTGCCCTAGCCATTAGAATGTGTGTTTCCACAACACTGGGCTAGCCAAGCAATCTAGGAATTGAAGAATGAAGGCACACGGGGGTTTCAGATCTGTCATTGGACCAAGTCCCTGCCTGCCTGCCTGTCTGTCTGCAATAGAATGCTCAGTTCATGGACTTTAAGCATGGAGATGTAAACTATCTAAAGTATACACTGTGGAAGGAGCCCCTGTCTCCAGCAGTAGCAGATGCTCTTCCTGGCCTACTGCTGGGGTAAAAATACCATCTTTAGGATGAGCCGGTACGAGTAAAGTTGGAGTTCAGTAAATACAGAAGGGAAGTCTGGGACTTGTTAAGGGGAGCAGGGCGGGCAGGAAGGGGATTGCAACACTTGGGATGTAAATAAATAAAAATCCATTAAAAAGAAGACAATCCATGCTTGTTTAAGCATGGGCATGGAGAGACACACAGCCAAGGAAAGGACAGAAGGACATTTGAGGAAATGAGGGCTTTTCAAGAGTGGAGTCATGGGGATTCTGGATAGTCCCCTTAATGAGCAAACTTAATGGGATGGGGGTTATTTGTGAGTTAAAACCGGGTGGGAGGTAACAAGTGTGGATTTCCCCAGAACAGGGTTCACCTCTTGTTGTCCTTCTACGGGGCTTTCCAGATGTGTCGTGGCATCAGAGGAATAATGGGAAAAGCAGTCTTACAAGCCCAGTGGTGGATGGGGCCATTACAGAGAAGCCAAGGATGTCAGTAGGCATATGTAGTCAGCCATGTTGGTCCCAGCGGGGCTTGGGTGGCCGTGTTCTTCACAGCATCCTGATAGCAGACAGCTCTGGGCTTTACCTTCTCTTTAAGCAACAAGTAGTCGGCCTTTCTCCACATGCCTATGCAGCCTGTCTCAAACTCCCCTACCTTTGGTTGAAGAGCTTAAAGACATCGCCTTTTGTAGCTTGCTTTGAGATATTGATAAGTCTCTTTACTTCTCTGAGCTTCGGTTTCTTCTTTTGTGGAAGGGCAGGGGTGGGTTGATTTGGAAACTGGAGATTTCCAATACCTTGGGGTTTAGTTTGCTATTATTTTCCTCCACCCAAGACACTCAGAGCCTAGAAGAGAGAGCTAGGAGCTTCAGTAGCTGTAAGCCTTCTTCTGATCCTGTGCTGGGAATCAAACACGGGTCCTCTACAAGAGCCAGGCATGGTCTTACGCTCTGAGCCCTCTCCAGGCCCACATTTACTCTTGTTAGCAGCACCAATCCATTTCAGAGTGGCCCAAAGAAAAGCAACCAATGAATACATCTATAACGAGAGATTCATGTTGGGCTCAGGTACCTATGATTTCATAGGCCCCAGTCTGCCAGGCAGCCCAAAGGCTGCAGACTCTGGCTGGAGTTAGTGTGGACTCTTGCGGCCCCAAAGCTTCTCCCAGTGGGCATGTTCAAACTTTTGGCTTGTCAACGTGGCATTCACACACATGAGAAATAGGCAATCAAGTTATAAGACTAATTTCAAAACTCATCATATTTTAAGTAAGCTTCTGCCCTTAAATATTTGTTATTGTTGATACTAATGTGTCATTGTGGCTTCATTTGCACTTTTCTAATGATCAGTGCAATGATACTGATCACGGATGCACTGATCATTCCCTAGGGTATAATGATATTGAACATGTTTTTCTGTGTCCGTGGGCCTTGTGTACCTGCTGTAGAGAAATATCTATCCAAGTCCTTTGCCCCTTTGCTGATCTGGCAATTCTGTTGGTAAGAACATTTTGCATTTTAACTCTCTACGAGATAAAGAATTAAAATATGAATTCCTCAAGGGCAAAAAGATATCTTTCAGAGTCTTTTAATTCTGACAAAGTACCTGGAACATATTGGCTCTCCAAGGAACATTCCAGAAAGGCCACAAATAAACTGAAATCATCTCTGTGAAGAGAAAGATGAAACCAAGACCCAGGGAAACCCAGGGACACGGCGAGATCCCAGCCCAAAGAATTGTCTCTGAGGTGAAGGGAGAAAACACAGGGATCTAACAGGAACTGGTTAAGAAGACGAGGCAAATTCCACTCTAAACACCACCCGAAAGAGACACTGTGAGGGCCAGGCCATGGCAGTCAGAAGGCCACGTGGACCCATCTGGAAGATGGCTTGCTCAGGACCAACAGACAGACTCTGGGCATCTGAAAGGCCTTTAAGGATAAAAGTAAGCAGAGCATGCCTCATGCTACCTCTGTTGCCAAGAGATCCCTTCTGAAGAGGGAGGGAAAACTGCAGGCCCCAGGGATCTCTCTATACTTGGTGGACCAGTAGAACCTAGAAGGACCAAATCCTTAGCCTGGACAGATATGATGCAAGGGGAACCCCTTTTCCTGAGATGATTGTAGTTTATGTATGGCTCGTGGAGATGCAAACAGGAATTTGAGTGAGAGGCCACTTCTTGCCTGGGGTGTTATGAAGATGATAAAGAAGGCAGAGGCCTCGGCCAGAGCCCAGCCCCTCCTTGTGTTCAGGAGAGGTCGTCTCCCACTGTGCCTGTGAGACACTGCCCTGCCCCCCCCCCCCCAAGGCAGCAGAAGTGAGGGGTCAGGGCAGCTTGCTTCTGCTTCTACTTTAGTAGAAAACCTTTTCCAGGTACGAATTCAAGAACATTCCAGTGGGGTCCAGCTTCTCGCGGATGTCACAGAACTTGTGAAAGGCGGGGTACATTTTCTCAAAGTCCTTCCTGGTGCAATTGTGGGCCTGTGGACACAATCAGCAGCATCGGGCTTTGTCCTCACAGAACTATGTCAAGCAAGGCTGCACTGATCCTGAGCCTGCACTGACACTGCCCCGCCCTCCCCCTCCCCCGACAAAAAGAAGGGGGTCTTGAAGATAACGCTAAAAGGCCTTAAAATGCACCCCATGATAGCATGGAGGCCCCAGCTTCGGTCCCACCACTACACAGGCCTTCCTTAACTGTTTTATTTGTTAAGTGGGCACACTGATGTTTACCTTACCCTGCTCGCTAGCTCCATGGGAAAATCTAATAAAGTGAAAGAAATCTCTAGGATGATTCCCAAGGGATCTGGGCTTCAGGGGCCAGAGTGCAGGAAAGGCATATTCTGGTGCCCTTTCACTAAGAAGCTGGGAGGATTCCTCAGAGTTAATAGGGAGGAGCGCCCTCTTGTGGACATAAGGTGCACAGCAGTTGCCCCGTGGCTATGGGGAAATGTCTGAATGTTTTTACCTTTGCCCAGTGGGGCCTGCCTCCAAACTTCTTCATGATGGTCTCATAGGCCAGCCAGTAATCCAACCGAGGCACATCCTTCCCATAGGGCCTAAAGTAGAGAGGAAGAAGGTGGCTATCCGGCTCTGTCCCATGATGTGCCTCCACTGTCTCAGGTGCCTTCTCTTTGACCTGCCCCTAATCTGGCAGGTGACTGGAGATTAAGCTGGCTCTCCCTGCAGATCAGCCCAGCCAGGACAGATGCCTGCTCACTGCACATCCCTAAGGGTTCCCCGAGACGTTGTCCCCTCTTGTATTCATCAGTTGGTATCTGGACATTACTGTGAGTTTAATCTTGGCCAAAGACTGACCAAGGGTAATGGAGCTGAAAAATTCCTCTTGTCCAGGTTATCGCTTTACACAGGTTAGCCACCATCATATACAATAGATATAATGTATCGCACACCACAATCATATACAATAGATACAATGTATCGCATCCCACCATCATATACAATAGATACAATGTATTGCATCCCACCATCATATACAATAGATACAATGTATCACATGCCACCATCATATAT
GF3 -1 -1 1 CTCTGCTCTGCGCGCGTAATTAAGC
GF4 -1 -1 1 CTCCTCTGCTCTGCGCGCGTAATTAAGC
EX52 -1 -1 1 GGTATATTAATAAGGAGATTCAGAACGCCG
EX32 -1 -1 1 CTTCACTCCCTGGACGGCGTTCTGAATCTC
GR3 -1 -1 1 GACTGTATGGAAGGACGGTGATGTGAGATG
GR4 -1 -1 1 GAGGCCTACTCGCCTGGAGACATG
Comments:
Target: otter_flex OTTMUSG00000032654 -- 64927435 -- 64927583 --
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: , start : end
Couldnt find RP24 black6 bacs at NCBI36 location: chr14-02, -6000, 6000
Found less than four RP24 bacs: looking for extra from RP23
Couldnt find RP23 black6 bacs at NCBI36 location: chr14-02, -6000, 6000
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_501 G3:chr14-02:64934083:64935082_473
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_501 G3:chr14-02:64934083:64935082_481
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_501 G3:chr14-02:64934083:64935082_712
REJECT (2) gap pair G5:chr14-02:64924935:64925934_701 G3:chr14-02:64934083:64935082_473
REJECT (2) gap pair G5:chr14-02:64924935:64925934_701 G3:chr14-02:64934083:64935082_481
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_701 G3:chr14-02:64934083:64935082_712
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_506 G3:chr14-02:64934083:64935082_473
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_506 G3:chr14-02:64934083:64935082_481
ACCEPT (0) gap pair G5:chr14-02:64924935:64925934_506 G3:chr14-02:64934083:64935082_712
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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