High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 42920
Chr: 7
Strand: 1
Status: Ordered
BACs: bMQ433b03 : RP24-546H20 : RP24-123D19 : RP24-194O7 : RP24-218O12 : RP24-194L4 : RP24-332B18 : RP24-486C19 : RP24-266F8 : RP24-280P6 : RP24-461N19 :
Instance: Plate 84: Well D01: BACs RP24-546H20 RP24-123D19 RP24-194O7 RP24-218O12
Instance: Plate 213: Well A01: BACs RP24-546H20 RP24-123D19 RP24-194O7 RP24-218O12
Oligos on GRCm38:
Type Start End Length Seq
G5 141350829 141350878 50 GAGGCGCTATCATTTGTCTAGGCTATGACTTCATTTTTGATCCATAAGTC
U5 141355169 141355218 50 TTCATCCAGATGTGTGTGGCATGCTGGTCTGTGTAAGGGAATTGAGCCGA
U3 141355295 141355344 50 TGCTAGGTTGCTAAGCAACCAGGGCAGCACTGTTCTCTGGAAGAGCCTTG
D5 141358449 141358498 50 TAATTTTAATTCTTATTGGAAATAGCTTATCATACAATATACTCTGATCA
D3 141358607 141358656 50 CAAAACCAGCAGAAGGGAGAACCAAAGAAAAAGTACAAAAAAGTATGAAA
G3 141363571 141363620 50 TGCTGGTAGCTCCTTATACATCTTTGGGTGACTGTTAGATACCTTGTATC
Oligos:
Type Start End Length Seq
D5_D3 141209819 141209926 108 TGGTTTCCCCTCACCTAACTCCTGCCAGAGCCTCCCCACCCACCCACATCCACACTCTTTATTTCTCTCTCACACAACAACAACAAAATATAGCAAACAAGAGTAGAA
G5 141202149 141202198 50 GAGGCGCTATCATTTGTCTAGGCTATGACTTCATTTTTGATCCATAAGTC
U5 141206489 141206538 50 TTCATCCAGATGTGTGTGGCATGCTGGTCTGTGTAAGGGAATTGAGCCGA
U3 141206615 141206664 50 TGCTAGGTTGCTAAGCAACCAGGGCAGCACTGTTCTCTGGAAGAGCCTTG
D5 141209769 141209818 50 TAATTTTAATTCTTATTGGAAATAGCTTATCATACAATATACTCTGATCA
D3 141209927 141209976 50 CAAAACCAGCAGAAGGGAGAACCAAAGAAAAAGTACAAAAAAGTATGAAA
G3 141214891 141214940 50 TGCTGGTAGCTCCTTATACATCTTTGGGTGACTGTTAGATACCTTGTATC
G5_U5 141202149 141206538 4390 GAGGCGCTATCATTTGTCTAGGCTATGACTTCATTTTTGATCCATAAGTCATGTTTTCCATATGCCATTCACCCATCCATCGTTTATTCACGTTTCTGAGGTTTCACTCTCTGGCCCAGCCTGGCCTGCACCGCCTGGCCTCCCTGCTTCCATTTCCCAAGCGGAGGGATCACAAGCATTCATCACCATGCCCAGCTTTGAGAATTCTTTAAAGTTTTTATTTATTTGTTTGACTGTTTTTGAGACAGGGCTCCATAGAGCCCAGACTAGCTAACCTTGAACACTGTGTAGATGAAGTGAACCTTCAACTCCAGGTTCTTCTGTCTGGTATCTTGCCTTGTTGAGATTACAGCCCTGCTCTACCACACCCTGCTGAAGTTTTTTGTATTGATCTCTGCTCTAATTCCACCAAGGTCAAAAACCTCTGTGCTTTCAGTCTTGTGGCCTTTCCTGAGACTCCTTTGGTCGCCTAGCAGCATGACTTTGTTTGGTTAGTTGTGAGGTGTTTCGTTTTAGTGTTTACCCTGCCCGCTACAAATGGAAGACTCGTCTCCCATCCCACTGCGTTTCTGCCTACTCATTGTTGAACCTGTTTGCAGCCGGCAAGCTGGCCTAACTCTTGCATCTGTGCAGGTTACCTACAGTAGTTTTGCTAGGGGTAGATTCATACAAATGCTTTTCAAGGTGTCACTAATTTGTCAGTAAAGAGGCTGGGCAGGAGCTAGATGAGAGCACTGCTTCTCTGTCTAAACACACAAGTGTAGCTCACGGGGACTTTGGGCAATGGATAGGAGAATGTGCAAAACTCAATAGTCATTTCATTCCTGAAACAATAGTCTCCTAAGAGTGTCAGTTTGCATATTTTTTATTCAGTCAGGCTTCCTGCATTTCACTTTGTTAAAAAGTTACATTAGTGAGGCTGGAGAGATTGGTTAAGAGTTTTGTTGCTTTTACAAAGAACTCAAGTTTGTAAAGTTACACCAGGGACTGGAAAGATGGCTCAGAGTTAAGAGTGCTTGCGCTTACAGAAGATTTGGGTTCGATTCCAAACACCCACATGGTGGTTCCTCTAGTTCCAGGGGATCGGATGCCCTAGTCTGACCTCCATGGGCACCAGGTACTGGTACACACACACGTATGAAGATTAAAATATATATATATATATATATATATATGTCAATCACACCAAACAAGGGTGTTGTGTCACATGCCTGTAATCCCAGCAGCACTTGGAAGACAGAGATAAGAGGATCACTACAAGTTCAAAGCCAGCCTGGGCCACTTGTCAAATTCCAAGCTAGCCAGAGCTACATAGCAACACCCTCTCAAAACAAACAAACAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAAAGGTCCTGAGTTCAATTCCCAGCATCCACATGGTGGCTCACAACCATCCGTAACGAGATCTGGGTGCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATAAATAAATAAATCTTTTTTTAAAAAGTGTTTAAACAAACAAACAAACAAGAAGAGATTATTTCTGGTTTGAAACCAGATTTGTTTTTCAGTTTTTTCTTGATAAAGGTCCATTCACTCTGCTTAAAAAAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAAAAGAAAAAGAAAGAAAGAAAGGCCTGGCTTAGTGGCATGGCACACCTTTAATCCCAGCAGAGACAGGTGGATCCCTGAGTTCAATGCCTCATCTACAGACCAAGTTCTAGAACAGCCAGAGCTACATAGTCTTCAAAACCAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAGTATAAAATACCTGAGCCATGTGTTGGTTACGTACTCTTTTAATCCCAGCACTTGGGAGGCAGAAGCAGGTGGATCTCTGAGTTTAAGGTCAACCTAGTTTACAGAGTGAATTCCAGGACAGCCAGAGATACACAGAGAACCCTTTCTCAATAAATAAATAAATAAGCAAGCAACTAAACAGATATATAAATGAATAGACTAGATTAAGTGGCTGGGTGTGTACCCACTTGCCTCTCATTCCAACACTTGGGAGGCAGAGGTAGATGGGTCTCTGAGTTCTAGACTAGCCAGGGCAACAGTGAGACCCTGTCTCAAAATAAATAAATAAAATAATAAAATATAAAAATAAAAAAGTTACTTATAACCCAGTGTGGTGGCACACACTTGTAATCTCGGCACCAAGGAAGTTTGGACAAGGAGTGAGTTGTGAATTCAAGGACCTGGGCCATATAGTGAGACACTGTCTCAAAATCCTACTCCAGGGCTGGAGGAATGGCTCAGCAGTTAAGAGTACTTACTATCTGCTCCTCCAGAAGTCCAGAATTCAATTTCCAGCAACCACATGGTGGCTAACAATCGTCTATAATGGGATCTGATGGCCTCTTCTGTCTTGCGGGCATACATGCAAATACAGCACTCATGTACATAAATAAATCTTTTCTTTTAAGCTGTACTAAAAACTTGCTTATTCTTCTGTAGCCTTGGTGCTCCTTTCCTTCAGTTATTTTGTTAGGTGTTTCCTTTTTATCTTGGTTTGGTTGTTCGGGGGAGGTTGTTTTCAAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGCACTTGCTCTGTAGACCAGGCTGGCCTCTAATTTGCAGAGATTCGCCTGCCTCTGACTCCTGCGTGCTGGGATTAAAAGGGTTCACTACCACGGCTCAACTGCATGCTGAACTCTTTATAAATATAAACAAAACTAGCCGGGGGCTGGAGAGATGGCTTTTACAGACATCCCAAGTTTGGTTCCCAACACCCACATCAGGCAGTTCATATCTCCCTCTACCTCTGGCTCCAGGGGTGCTAATACCCTCCTCTGGTCTCCCCTGACACCAGGCACAAACATGTTATACACATACATACATGCAAGCAAAAACAATCATACATAAAATAAAAATAAAGGAAATAAAAAGAAAACAAGCCCAAATCAAGGAGAGTGAAACCCCAGGAAGCTAAGGTAGAAGGATTACTGTAAGTCTGAGGACATTCTAAGCTACATAGTGAATTATAGGCCAGCCTGGCTACAGAGTGGAACCTCCCTTCAGAAAAGGGGCGAGGGTCTTGGGGTGCAGCTCAGTGTGTACAGTATTTACCTAATATGCACAAAGCCTTGGGTTTGATCCTCAGTACCACAGACCCATACAAATATGGCAGCATAAGCCTGCAATCACATCACTTGGGAGAATCAGGAATACAAGGTCATCTTCAGCTGCATAGCAAGTCTCAGTTGAGACCAGCCTGCGTTAAATGTTTCTCATATATATATGAAACAGGGTGTCAGTGGGTGGCCTGAAACTTGCTATGTGAATCAGGCTGGCCTCAGCATCACAGAGATGTATCTGTCTGCCTCTGCCTCCTGAGTGCTGGGATTAAAAGCATGTACTACCATATCCAGCCAAGTCTGGAAAATGGTTTAATTTTATTATTTCACATGTATGGGTGTTTTACCTGCATGTATGTGTCTGTATGCTACATGTATTACTTGTGGCTATAGAGGTCTGAAGAGAGCATCAGGTCCCCTGGAACTGGAGTTACAGAGGATTGTGAGCAGCCATGTGGGTTCTGGGAACTGAACGCTGATCTGAAAGAACAGCTACTGGGTTTTTTTGGGGGGGGGTTGTTTTGTTTTGTTTTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACGCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCAACACCACTACCCGGCCAGCTACTGTTCTTAACCACTGACCCATCTCCCCTGTCCCAGTCTAAAAGTTTTTGAGGACTTTAGTCAGCAATCATAAGCCACAAAGGCACACACCAGAGCAGAAAGATGACTTCTCCCGACTAAGGTGTAGCTGTGGCCCTTCAGGATCCCCAGTTGGCTGAGGCTCCCAGACTGTCCCCTCTTGTGCCCAGGGTTTAAGTAGTAGAAGAGCTGTTCCTGGCAGTGACCTCTGACCTCTCCCTTAGCACCTGGCCACATTCATCATGGACAAGAGTGAGGCTATTGCCTCCGTGGATGACGCCATCCGGAAGCTGGTACAGCTGAGCTCCAAGGAGAAGGTCTGGGCCCAGGAAGTCCTGCTGCAGGTGAACGACAAGTCACTGCGACTGCTGGATGTTGAGTCCCAGGTACTGGTTGACAACAGCAGGTACAGGGAGCTGACGGACAGAGGCAGGGCCAGATGTCCAGTTCATCCAGATGTGTGTGGCATGCTGGTCTGTGTAAGGGAATTGAGCCGA
U3_D5 141206615 141209818 3204 TGCTAGGTTGCTAAGCAACCAGGGCAGCACTGTTCTCTGGAAGAGCCTTGCTGGGTATCAAGCGAGATACGGCTGTGTCCCAGGGCCTATGCTCGAGGGCATCCCCAATTAGTGTCTATGTCCCTGTCCATGGGCCAGGTGTGGGCAGTGTTCCCGTGTTACACCTGTGAAAGTTACACATGTGTTCCTATCTGGGGAGCATATTTCACATACCGTGTCCAGAAGAATCCATTGCTGGGGTGTCAGTGTGTTTCTCAGCCACATACATAGCGTTATTTATCATGATGGTAGCCTACATCCACATCCGAGGCCTGTCAGGCAAGGCTGATCCACCGTATACCCTCCCACAGGAAGAGCTGGAGAACTTTCCGCTGCCCACTGTGCAGCACAGCCAGACAGTGCTAAACCAGCTGCGCTACCCCTCTGTGCTGCTGCTGGTGTGCCAGGACTCAGACCAGAACAAGCCCGACATCCACTTCTTTCACTGTGACGAGGTGGAGGTAAGGCAGACAGCCAGGGGTGGGCATGACGCCATGCCAGTTGGCCAAGCTGACTTGATACCCCTGTGTCTCCAGGCGGAACTTGTGCAGGAAGACATTGAGAGTGCTCTGGCTGACTACCGGCTGGGGAAGAAGATGAGACCACAGACCCTAAAGTAGGGCCCTGGGGGTGTAGAAAGTGAGGGATCTTGCCCTTTTGCTTGGGGCACAGTAGCCTGGGATGTGAGGGCAGCAGGGTGGCAGCATCCAGCTGGAGGGCCATGGCCAGGCTAAGCTATGTCTTCCTCTGCCCTCTAGGGGACACCAGGAGAAGATCCGCCAGCGGCAGTCTATCTTACCCCCTCCACAGAGCCCAGCCCCCATTCCCTTCCAGCGACAGCCAGGAGATTCCCCTCAGGCCAAGAACCGTGTGGGCCTGCCCTTGCCAGTGCCATTCAGTGAACCAGGTAGGCTGAGGGATGGGTGGGGATTCCATTTGGGATTTAATGGAGAACCCTTCAGCAGCTGCTCTGTACCCCCTCCCCCAACAGGCTATCGCAGGAGGGAATCCCAGGACGAGGAGCCACGGGCTGTGCTGGCTCAGAGAATAGAGAAGGAGACGGTGGGTGCCCAGCCTCCCAAGACTCTTTTCCATCCTTGCCTGCTTCCTGTTGGCACTGGGTCTGGCTCATGGCGGCCAGAGTGTCCCCACAGTGGGTGGGGGATGATCATGGCTGACCCCTGCTCCCTTTTCTAACCTCAGCAAATCCTTAACTGCACCCTGGATGATATTGAATGGTTCGTGGCCAGGCTGCAGAAGGCAGCTGAGGCTTTCAAGCAGTTGAACCAGCGGAAAAAGGGGAAGAAGAAGAACAAGAAGGGGCCAGCAGGTACCAACCAGAGTGATAGGGAAGGGGCTGGAGGATGCTGGGGCAAGAAACGGTAGGCACGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTCCGAAGGTCCGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAACAAGATCTGACTCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAATAAATCTAAAAAAAAAAAGTTTAAAAAAAAAAAAAGAAAAAGAAACGGTAGGCACACACTAAGCTGGAGGGAAGGGGAGTCTGGAAGGGGACAGCAACCAACAGCAGGCACTCAGCGGGGGTCCAAGGCTCACAACTTTCCACGTTTTGCCAGAGGGTGTCCTTACACTTCGGGCACGCCCCCCCTCGGAGGGAGAGTTTGTTGACTGTTTCCAGAAAACCAAGCTGGCCATCAACCTGCTGGTGAGTCAGCCCCGCCCTGTGTTGGGGGTGCGGGTAGGGTGCAGGCCCACACACATGCACGCACACGCACATGCCATCTGTCACCTGCTTGTTCTTCCCACCCCAGGCGAAGCTGCAGAAGCACATCCAAAACCCTAGTGCAGCCGAGCTGGTGCACTTCCTCTTCGGGCCTCTGGATCTGGTGACTGGGGCTGGGACAGGGCGGGGTCGGGGGCAACAGAGTGATCCACATGGGTTTGGGGTGGAGGAAACGAGTGCCCACCTTAGCATACTCAAGCTCTGTCTGGGTTCCCAGGAGGCCACAGTCCATCCCTGAGGGCCGGCCTATAGAGCTGTGGTGGGTGGGGGTAGGCTGGGTCTGGTGGCTTTGGTGGGCTGGGCTTCCCCACTAAACTCACTCTGTCTAGATCATCAACACATGTGGCAGCCCCGACATAGCACGCTCTGTCTCCAGCCCTTTGCTGTCCACTGACGCCGTGAGCTTCTTACGTGGCCACTTGGTCCCCAAAGAGATGACACTGTGGGAATCACTGGGGGAGACCTGGATGAGGCCTCGGTATGGAGAAGGGGCAAGATGTGAACTAAACAGAGGTCTCAGGTGGGTTGGTCCCAGCAGGGCATGTGACTGACCTTCTGGCTGCTGACCACAGCTCTGAATGGCCCCGGGAGCCACAGGTGCCACTCTATGTGCCCAAGTTCCGCAGCGGCTGGGAACCACCCCTGGATGTCCTACAGGAGGCTCCCTGGGAGGTAGAAGGACTGGCATCTGTGCCCAGTGATCAGGTCAGAACTCCAGCCCAGCCCATGCCCACCCCTCCCCAGAGCTTCTCCCCAGCCCACCCGTGCTTCTACCTTCAAGGCTTCTAAAGCAACAATGTCTTCTACCCCAACCCCTACAATGCCAGGTCACCTTGGCTTACCACCTGCCTGGGCATTTGGCCCTTTGTCCTCAATGGTGCTAGGTCACAGCACATTCATCAAGCTGTGTCCCTTGTCCCAAGACTGATCACAGTCACTGTAGCCCATCCGCTACCCCACATCAAGCCCAGGTTCCCTGCTTTTTCCACAGCTGACCCCCAAGAATCGACTATCTGTACGACATTCCCCAAAGCACAGCCTCTCCTCCGAGTCCCAAGCCCCAGAGGACATTGCACCACCAGGCAGCTCCCCACATGCTAACAGGTAAGTCCCCACAGTGTGAGCACCACACAACTGTTGCATGTGGGCTACCTCCACACGGTGTCAAATGCCCCATTCTGGTATAAAATAGTTATTTTCCAAACGAGAATCCAACTTTCTTTTTGTTTTTAATATAATTTTAATTCTTATTGGAAATAGCTTATCATACAATATACTCTGATCA
D3_G3 141209927 141214940 5014 CAAAACCAGCAGAAGGGAGAACCAAAGAAAAAGTACAAAAAAGTATGAAAAATTCCATAAAAACACAAAATTGGAAACCATTTTACATAAGCAGAGAACTGTGATGTAAAAGCAATGCCCAACGCATCGCCTGACAGAAACCTCAAAAAAAAAACAAACAAAAAAAAAAAAACACTGAGCTTGATTTGCGCTAGCCACCGATTTTCTTCTTAGCTTTGTCTCTTTAATGATTTATTTGTATTTTATGTGTATTGGTGTTTTGCTTGCATGCATGAGGGTATAGGATCCCCTGGAACTGGAGTTATAGACATTTGTGAACTTCCATGCGGGTGCTGGGAATTGAACCTGGGTCCTCTGGAAGAACAGCTAGGGTTTTTAACCATTGAGCTATCTCTCCAGCCCCTCTTCTTAGCTTTGTAACATATAATTGAAGGAAACTTTTCAAGTCACATCTGCTTTCAAAAGTCTTAATCTATATCGGTCCTTTTTCCCTTCCTTCCTTCCTTCCTTCCTTCCTTTCTTTCTTTCTTTCTTCCTTTCTTTCTTTCCTTTCTTTCTTTTTTTCTTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTCTGGCTGTCCTTCCTGGAACTCACTCTGTAGACCATGCTGGCCTTGAACTCAGAGATCTGCCTGCCTCTGCCTTCTGAGTGCTGGTTGCCTGGCTACATTACTTCTTTCTATACTTTCTTGTTTCATAATCAATGTTAGAAAGGGACAGAGATTGGATGTGGAAGCCCACACCTTTGGTTCCAGGACTCAGGAGGCAGAGGCAGACAGATCTCTGTGAGTCTCAGGCAGTCAATGGCTGCATAGTGAGACGATGTCAAATTTTTAATTAATTAAAATCAAGGTTAGAAACTCACTGAAGGAAAAAAGGAAAGAAAGAAACAAACACCACCAGCGACTCCTCCAAGCACCACCCACTATGGTGCTGTTGGGGATTCAGAGCAAAGGGCTCTGATTATCCCCCACACACACACTAAGCATCCCACACTCCCCGTGAGTTAAACAGAAGCCCAAGACATGGAGGTATCCCTCTTACCTCCCCCTCCTCTGGTTCTTACTCCTACCCAGGGTCTACCTGGTGGTCCTACGGCTCCTTTTCAAAGTGAACCCCTCTCCTTGGGCTTCTTTAGCTTGCTATCCAGTGTCCCTACTGGGATCATATGTATGGGACATTCTCAGCCTATCAATACACTGGGCACAGACCTGGATCCCCCACCCCTCCTCCCTCTCCAGTGCCACACTGACCAGGAACATGACCTGGCTAGGGGGGAAACCATTAGCCAGAGCTCACCCCTGCTCCAGAGATGCAAGGGGACTTCCAGAAAGGACACATAATATCTCTAAGGGGCTGTAACTGAAAATCTCCTAGCCCTAGCCCTGTGGTCAACTGGGAACATGTGACCCTGTCACTCCCTAGGCACACCTCTTGCTCTAGTCCCTCTGAGGAGCAGGGCAAGAGACTCCCCAAGATAGTTTGAGCTGGGCCAGCCTCTTGGAGTCTCGTCTCCCCCCCCCCCCCCCCCCCCCCCCGCCTGCTGGAGTGTCACAGTTTCCACTCCCGGCTGAGAAGCACAGCCTCTCTTCACTGCAACCTGGGCAGCAGAACAGGAGGCAGCTGCAGCTGGGAAGCAGGAAGAGACTTCCGTCCAGCCCTGGGTCTCAACTCTTCCTGGTCCTGCAGGGGCTACCAGCCAACACCAGCCATGACCAAGTACGTCAAGATCCTCTATGATTTCACAGCGCGAAACGCCAATGAGCTATCTGTGCTCAAGGACGAGGTCCTAGAGGTAAGGACCGGGGCTATACAGGGGAAGTGGGGCGTGTTCTGGGACCATGGTCTGTAATCCACCCATCTTCCCAGTGTGCTCGACCTGGCTTCCTACTGGGCCTCCAGTGGCCTCTCTGACCCCCACCACAGTCTGGCTGAGCCACTGAGCTGCCTTCTGATGTCTGGGGGATCCCTGGAGGTCTACCGTGGTGGGTGGCCCTTCGGTAGAAGCCAGTAGGGGCTTGTCATTCATGGCCACCCAAGATCACCACCCTGGACCAACTTATTTGTGCCTAACCTGAATCCAGGCCACCTACTGAGGGACAGAAAGTGGAGCAGGCTGGGGGAAGCTCTGCATTTAGAATCAGGTTAAGAGGTACCCTGTCTCCAGGACTGGGGAGGCCAGGCAGGCACTGGGTAGCCCATCAGGCCAAGACATGTCCAGAGGATGGAGAGCTGTGCTTGAGATACCTGGACAATGCTTTGGCCCTCTGTGAAGGGTTTGGCAAGTGTCCCCAGCATCTGACTCTAATAGTACTTGAGGTCTGCACGAGGATGTTGGTGAGTCTCACAGACATCTGGACACTTGGCAGGTGCTGGAGGACGGCCGGCAGTGGTGGAAGCTACGAAATCGCAGTGGCCAGGCGGGCTATGTGCCCTGCAACATCCTGGCTGAAGCTCGGCAGGAGGACGTGGGTGCCCCCTTGGAACAGGTGATCGCCTGCCACGGCTCCATTCTGTCTACAACACAGTCAGAGCCCCTAAGGTCAGGGGTGCAGCTGGTCTCAATGTGATATTTGAACCTGATCCCCGGAAACCTGCTGCCCCCAAGCTGGGCCAAGGGCTGTGTGGGTTCCTGGGGTCTTACTCAAGGGAAGAAAGGGCTGGCCCATCCATCTGGGGTCCTGGGTAAGTCCTTATATAATAGCCCCCCCCTCACCTTACCCCTCACCTCTTCCTTACTCCTTGCAGTCTGGCCAGAAATACTGGGGTCCAGCCAGCCCCACCCACAAACTGCCCCCAATTTTCGCAGGGAACAAAGAAGGTAAGTAGATCCACTTTGGGGTTAGAAGTGTAGGGAGGGGCAGGGGAGGGCGCTGATAGGATGCGGAGGGTGGGGAAGAGGCAGAGAACGGGTCGAGTGAAGGGGCTGAGAGCGTGCGCCCCACAGAGCTAATCCACCACATGGACGAGGTCAATGACGAGCTCATGAAAAAGATCAGCCACATCAAGACACAGCCGCAGCGCAACTTCCGCGTGGAGCGCAGCCAGCCAGTGCACCTGCCGCTTACCTTCGAGTCAGGTCCGGATGAGGTCCGCGCCTGGCTGGAAGCCAAGGCCTTCAGTGCCAGGTGAGTCCCGTGTGTACAGCCGGTGGGGCGGAGCCTGGAGGACAGTGTCCCGCCCACGGCCCCGCCCTCACACCCACCTGCCCCTAGGATCGTGGAGAACCTGGGCATCTTAACTGGACCCCAGCTCTTCTCCCTCAACAAGGAGGAACTGAAAAAAGTGTGCGGGGAAGAGGGCAGTCGCGTGTACAGCCAGCTCACGGTTCAGAAGGCCTTCCTGGAGGTGAGCAGCTCCTGCCTGCTTCTGAGTTCCTTCCTAGGCACTTGCACCTGGGGTCACTGGAAAAACAGGAAACAGCAGGGCCCTGTCCTGTACCCTCTTTATTCCCCAACCTCCCGGCTGAGGCCCAATCTCTTTTTCCTAGAAACAACAAAGTGGGTCAGAACTGGAGAAGCTCATGAGCAAGATCAGGAGGGCCGAGGACAGCTATACCAGCCAACACACTTCACCAGAGTCAGAGGGGGCGCCGCACCTGTAATGGGCCCAACCCAAATGCATGGAGTATTATTTTTATATGTGTATTATTTTATACCAAGGACATAGATGGGCTGGGTGTAACTGGCTTACCTGCCCTCCAGGCAGAGTCCTGTGTATGAGCCTAGCTACACCTGTGGGTAGCTCTTTTACCACCCGAGCCCTACCCCCAACCCAATACTCTTCTTCCAAAACCAAGGCCAGCCTCAGCCAAACCTGTACCTGGCAGTGCCAGTCCCTTCTCAACCTGCTGCCAAGGCCCCAGGACTCTCCCGGTGGGCAAGGCCTCTTCCCCTGGTCTTCTTGGTGCACATGAAGGTCCTTGTTCCTTACAATGCTGGGCCCCGACTCTGCAGCTCTCCCTTGTGTTGCTTGTTTCTACATTCACGTTTTCCTGCATCCGCTGTACCCCAATTGCAGGATGGGCCTGAGCCCTCAGCTGACCAGCCTGGTGTCCTTGCCTACATATTTGCCCACTCCCTTCACGCTGCTAGCATCCCAGGAACAACCTGGTCAGGGGCTCCAGGACAGGAGGCCTTGGGTGACCTGCTGGTATAGAGTATGCTGTATTCTTTCTTCTTTGAGACCTAAGAATGAACCAGACATAGGTTCTGACTTCAAATGTCCCATCCCACCCAAAGAGCCTGTGATAACAGATGTCTCTGGCTATTTCCACCAGCCTGTTCGCTGGTGTCTGGTGCGTTTCCCAGCTCTGGATGGAGCCCCAGACCCAGAGATGAGAGTGGGGACTCCACAGCTATGTCAATAAACCAGATGTCTCCTAGCAACTTAGATATCTGTTTCTTGGGGTAGAAGTGCTCTGACACCCAAACTGCCTCTTCTTCCTGCTCCTTTTCTCTGGGATAGTGCATTGGCCCTGAACTCTGAATCGTCATCCTGCCTAAGCCTTCTGTGCTGGGATCCCACTTGTAGACCACCAAGCCTGGTCATGCCCTTCAAAGATCTTTCCAGTAGAGAGCAACCCACCCACACAGGTAGTGCTTTGGCCATGCCAGGGGACACCTCTCACCTCCACACCCATCCAAAATGGCCCTAAGGACATTTCTGGGAGGGACTCCAGAGGGTGTCTAGACAGCCCAAAACCTTCCAACCGGGTGGCTTTCAAAGGTCCCATTCTGAGTAGGAGCAGAGTTCAGCTGGCCCAGGAGCATGAATCAGAGGGAGATAGAGGCACTATTTAGAAGACTGTTCCAAGATGAGGGAGGTGTCAAAAAGAGACCTGGGGCCTGAAAGACCTCACTGACAAGTTGGAGAGAGACCCAGGTATCTGCCTGACTGGCCAGAGACTTTCCTTTGATGCAAGCCTTTGGTGCCATCTGCTGGTAGCTCCTTATACATCTTTGGGTGACTGTTAGATACCTTGTATC
U5_15 141191539 141206538 15000 GTGTGGAGAGCTGGAATTTGAACTTGGAGCCAGTTCCTCCGCCCTCCTTGCCCTGCCGGGGCCTCCTCCCGTTCCACTGGTGAACCTACCAAATGCTCTAACACGCTGGGCCTCCCTTCAACCCGGAAAGTGGCTACAGTAAAACCAGCCTGAGCCCCCAGGCCTTCTAGGCCTGGGTGTTACATCTTCACCACCAATGGATTGTTTGGATTTCCTGCCTCCCTACCCCCACTTCAGCTCTAGAAGCCTCCAGGGAGGGAACCTTTTGCAGGGTAGAGGTAGCCCCAGCCTTATCCCTCCTACCTAGTCCTAAGGTCCTGGCCTAGATTTCCCCAAGGGCAGGGCTGCCTGTCCAAGCCTGTTCAGAGTCTGTGTGCCTTCAGGCCTGGGTCTCTAGCAGTTTGTTTTGTCCTGGTGTGTGTATTCCTGGCCCCACGTGCCTGGTGCCCTTGGCTGAGCTAGCCAGGAATCCCATTAAGCAAACACCGGGGCTCACCTACTGAGGTCATGTGCATTCCGGACATTCTCCATGGATAGGTGGACCTAGAAATCTGGCCCAGCCTCTTCTTATAAGCTAGGACCTCGGCTGGGGGTAGGGGAGCAGCCAGAAAGCTGGTCAAGGTAATAGCTAAGGCCTACGGTCCTCGCCTCCTGGTAGCCCACCCCTCAAGACCTGGGCAAACCAGAAAATACTAGAGTGCTACAGGCCTACTCCTGAGCCCTCATACCAGTATCCTGGCTGAAGGCCTGACCCTAGCAGACTCAAATGCTGAAGCTAGGAAGGATGGTGGCCTTGTTAGTAATATCAGACAAAGGTAGACTATATGTGGGTCATTGGGAAGAGCTGGCCATAGAACTTCAGAAGGACAGAGAGAAATGTTATCGACCTGTCATTCCAGTGTCGTAACGGCAGCCTGTCACTGAAGGTCTCTCTACATCCCTCAGTTAATGTCAGGCTGGCCCAGCCCACCAGATTCTGTTGTATCCAGAGAAGGGCTCAGGTCGAGCCTGTAAGGAAAGTTTGCTCACATTCATGGCCATGCCTCAATTTCCCCAAAATGTACCCAGGGGAAGTCAGAGCTGGGAATTCTACTTCTGAAGAGGCCTTGTTTGTATATAGTCACCTGGGTTTTTACTAGGGGGGCCAGTCACCCCCAAAACTTCTGCCCTATACCCATCCTCTCCAGGGTCTCCCAAGAGGGAGATAAGAACTGGTCTCTTTGCTGGATTTGGGGAAGCTGGCCTCTGTGAGCATACCCACACATATATGCAGGGGTCCCTAGGAGTGAAGCCTGGGGCAAGGGAGGAGTAGTGGGGTCCACCTATACTTTGTGCCCTCCTAGGTCTCCCTCCAGCCTTACCCTCTCCTCTCTCACTGCCTCAGGGTTTTTGAAGGAACCATCTTCTCTCAGATTATCCCCCACCCAACCCCTCCATCAGAGCCTCCTGACAGTTTCTAGGAACACCCTTGCAGAGAACCAAAGGTCCTAGGCCAAATGAGTCTCCATTGCCACCACCACTAGCATGGGCCTGGAGGACTCTGCTTCCTAGAGATCCCCTGAGGACTCTGCCCAGCACTGAGTGTTCTAGCACCCGGGGCAGAAAGAAATAAGAGAGGAGACCCAGGCCTAGCCTGGCCAGGAGAAAGGGTCAGCTGAGCTCTTCTGTCTTTAGGTCTGCCCTCCCTGACACTGGCTACCACTGGGAACGTCATGAGCCAGTCAGCATCCATGAGCTGCTGCCCAGGAGCTGCCAAGTAAGTGTTGTCCTACCGCCTCAAGTCCCCAGGGAGGGGTGTGACATGAGCCCTGCCCTCACCTGCCTCTCCCGCTGCAGCGGCAGTCTGGGCCGGTCTGATGGTGTGCCCAGGATGAGCGCAAAGGACCTGTTTGGTGAGTGAAGTTTAAGGGTGGTATGGACTTAAGGAGGATGAACGTGACCATGGCGGTCTCATCCCTGCCTGCTCCGGGGCTGAGCTCTAGCCCAGGGAAGTTCTAAGGGCTCAGAGGAGGCCTCTGGACCCTACTTCACTGAGAACTCTGGATATCTATCTGCAAGGCTCCTGGCTACTAAACGTTCTCCACTCTGCTGTAAGGGGAGAGGCCTTTACGAATAATTCAGGGAGCATTAATATTGATTCCACTGTGCAGCCCTGCAATCCGATCAAGTAGGTGGGGTTAGATGGGACCCAGCTGCCAGGTATCTGCTCCAATGGACTGAGCTACTTCTGCTTGCCCGTGCCCCTCCCCAACTATGCCCTTAACTTGTGCTTCTGGATGCTGGATGCTGGAAGTTGGAGCCTTGGAAAGCAAGAACTCCAGAGAGGAGGCATGTTCCCGTGATAGCTTGTAGGATAGGAAATCCTTGGCCCGGGACCCTTCCATTAGAGGGTGGGCAGCCTCCATTAGAGAAGACTACATTTCCTGCCCCTCACCCAAACAGATCCTCAGTGAGAGGCTGCCCCCTCCCATTCTGAATAAGTCTCTTGTCTTCTAAAGCTTCAGGTCCCCAGTGGGGCCTGTGACAGGGCCAAGCCAGAGGCTCTCCTGGGGTTCCTCCCTGTCCCACGGGAGCTTCTCAGCCTTCAGGGGTTCAAAGAGCAGCACCTCTCATCTGGACTCTGTTCTCCCCTGTGGTCCCTGGGGGTCACGTCCTTCTAGACTGGGTCTTACTCTTTGGGGTGCATGACATCTGTGTCTCCATCCAGAGCAGAGGAAGAAGTACTCCAACTCCAACGTCATCATGCATGAGACTTCACAGTATCACGTGCAGGTGAGGACCAGGAAATAACCCCCATATTTCCAGGGACAGGTGGAGAAACTGAGGCTCAGGGAGAAGTTGGGAAGAGAGAGCAAAGGACTGTGCTGCGCTGGTCCTGGACCCAGCCATAGTGGGGAAACGGCTTCCTCAGGGCTGAGGGAGTTCATAGCCATAGCAGAGCCTGCTCAGACCCCTTCTCTGTAAGTTGGTACCCATGGCCCACAGCAGCCAGGGCGCTGAGACATCACCTATGTCGCTGGGCAGCTCGGAAAAGACCATGGAAGGAAGCTGTCATCTTGGCCCCAAAACAAGGAACTGCACTGGGAGGAACATGATGGAACAAAGACACTCTTTATTGAGAGCTACAGGGAGCCTGGATGGCAAGGCTGAGTCTAGACATAGCCTGTGCCCTTTGACCAGCTGCCAGCTTGAGTGGCCTTTCTCCTGGTCACCTCTAATTTGCTGAGAATTCAAGAACTATGTTTTAATGGCTTTCCTGGGACTGGGAGCTAGCTGGGTTTCTGGCCATAAACTGAGCTTATAAGATAGTGATGAGGAATGACAGAGAAGTAGAAAGAGGAGCCCCTGAGCAGCCAGCACTCCCAATGGGTCTCATGTATTGCTGGCTCTCTTCTGTCTCCCCACTCTACCCAGGACCCTTACCCCAGAAGTGAGGCAGGCTGCTATCCTTACCTCCTTGTCTGTAGAATGGACCAAAAGAGCCATCAGGAAATCCCCATGGCTCCTCAACCCTGTTCTCTTAGGCGATCAAGGTTCTGGCCACCCACCCACCTGGGTCAATGGGCCATGATCCTGTGACCACAGCCACTCAGTGCCCAAGGACAGGGCATGGAGGCACAGAAGGGTCAGCAATGACCTCTTCCTTTACAATGTGCCACCGTTCGTTCTCTGAAGTGGAACTTTGTCCTCCTTGGTGAGGAACTACGTGCGGCATCCAGCCTGGCTCCTGGACTTTGATCCATCCTGTTTGACGCTGCCCCACTGCTGCAGGAACCTCTCCTTCCTGCTCGAGGGCAGAGGCAGATGTTTCTGTCAGGTTTCTCTATTGCTCCCTGATGAAAAGACTGAGGCCCAGAGAAAGAGGCTGAGAAGCATTCAGAGCTGTGCTTGCTTTTTCTGACTTTTTCCTGACTTTTTATACAATAAATGGATGGTACAGTTCTGTTACTATCAGACTGGGACATACAAGAGTCCCATCTCCCCTAGGTGACTTTGAGAGCCCCTTGTGTCCAAAGCCACCCCCATCCTAATCCCTAGCAATGCTGATCTGTCTTGCATCCCCAACACTTGTCTTTTTGAGAAATTTACCCTATCCAGGAGATCCCATGGTCCACAGCCACCTTTGTTTCTTGCTTCATGAACTTATTTTAAGAAAACCATGCTGAGAGAAACCATGTCTCAAAACAAACAGAAACAAAAAAGCTAAAAGCAAACCTTCCTCCAGTTGGTAATTTGTTAGCTAATGTGTGTCAATATCTTTTGATAAACAGACATATTTGATTTGGAGGCACCTCGTCATAAACCCATTTTTCTTTTCTGGTTATTTGTTAATCTGCTAGTGAAAAGACTAAGTCTACTATTGAAGATTTCTGTGTTTTCAAGGTAGCATAATCTTTTGCATCTAGGCCCATGATCGACTTTATTTTGTTTTATTTTTGAGACCCTGTCTCTAATTAATTAAGTAGCATGTTGCTGACATTTGATTGGCATTGAGTCCAACCTATCGAGCTATTATCAGTTTAGGGAGAATTGACATCTTAACAATTTTGTGTGTTCTCATCTACTAGCATGGGATGTTTCTCCATTTATTAAGATTTCTTTAATTTTTTTTAACAGTGTTTTCTGATTTTCACTTTGGTGGTCATATTTATATTTAAATTATTCTTTAGCTTACAAATGGCATTGTGAGAGGTTTCCCTAGTACGGTGGTTATCATGTCATGTTCACCACATAAATAGGAGTGTGGTTTTAATGTTGCTTCGGTTTCGGTTTTGGTTTGGGACAGGGTTTCACTATGTAACCCTGATTGACCTGGAATTTGCCATTTAGACTAGGGTGATTTCCGGGGAATCCACCACCTTCCTCTGATCTCCCTGTGCCTGTGCCGGCACTGAAGGCATGTACCACCATACCAGGCACAAATGGCACTTCTTATTGTTGTTGTTGTTTTGTTTTGCTTTTTACTTTTTCTGGTGTTGTTTAAAAATTTTTTAGATTATTTATGTTATAGTATGAGTGTTTTGCCCTGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATATATGTGCAGTACATACATGTCTGGTACCCATGGAAGGCATTAAATTCCCTGGAACTATAGATGTAGACAGTTGTGAGCTGCCATGTAGATCCTGGGAATGGAACCAGGGTCCTCTGCAAGAACAAAAGTGTTCGCAACCACTGAGCCATCTCTCCAGTCCCTTTTCATTTTTTGAAATTATAATATAATTACATCATTTTCCCCCTTCCCTTTCCTTCCTCAAAATTCTCCCATATATCTTTTCTAGCTCTCTTTCAAATTCATGGCCTTTTTTTTTTTTCGTCAATTGCTGTTACATATATACATATGCATGTATATACATGTATATTCCTGAGTATGCAACTTGCTCAGTCTATACAGTGTTACTGGTGTGTATGCTTTCAGGGTTGACCATTTGGTATGGGATAACCTCTTGGTGTGCTGTGCCTTAGGGAAGTCAATCTCTTCAGCCCTCAGCATTCCTCACTTGCCGGTAGGTCTTGGTGTATGGTTGATGCTGAGGCCTTGTGGGTTTTCCCCATCTACTTTGGAATGTTTACTGTTGTTGTCCTTGTTAATCTCATGTTTGGCAGTCATGTTGGTGAGATGTGATGGGTGTAGCTTCTGACACTCCTAGGAGACACAGTCTCACAGCAAACTCCTGATCCCCTTGCTCTTACACAGTCCTTTCTGTCCCTTCATCTACAGTGCTTCCTGTGGGAGCTGTTTTGTAGATTTATCATTGGGACCGGGGTTTACAGCTCTCCATTTTGATTGGTTGTGGTTTTCTGTAATGGTCTCCTTTTGTTGCAAAGGAAAGTTTCTTTGTTGAAGGATGAGAACTACAGTTGTCTGTGGCTATAAGGACAAATATTTAAAATGTAACTAGGGATTATGCTGGTGTAATAAAGTGGCAGTAGTAGTTTCTCCTCCAAGATCTGTGACTTCACTAGTCCTGGGGAGCTGACTAGGTTTTCACTACTTGGCATGATGTCCCTCTTGCTAATAGAATCTTAGCCTAAGTAAACAGCTGTGGTGACCACCAAAGTATGTGCACCATAGATTGTGTTGGTTTTTTTTAAAGATTTATTTATTATTATTTGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGCGCCAGATCTCGTTACGGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCCGGACCTTCGGAAGAGCAGTCAGTGCTCTTAACCACTGAGCCATCTCTCCAGCCCCAGATTGTGGTTTGTTTGTTTTTTTTTTTCTTTTTTAATATTTTTCATTAGGTATTTTCCTCATTTACATTTCCAATGCTATCCCAAAAGTCCCTCATACCCTTCCCCCCACTCCCACTTTTTGGCCCTGGCATTCCCCTGTACTGGGGAATATAAAGTTTGCATGACCAATGGGCCTCTCTTTCCAGTGATGGCCAACTAGGCCATCTTTTGATACATATGCAGCTAAGACAAGCACTCCAGGGTACTGGTTAGTTCATATTGTTGTTCCACCTACAGGGTTGCAGTTCCCTTTAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGATCCATCCAATAGCTGACTGTGACAGATTGTGTTTTTTAAACTTCATCGTCTAATTATCTGCCGATTACATAAGCTAAAAAAGAGAGCTTTGGTATGGTAGTCTGAGACTTTGGTGAATTTAATGATTTCCTTGTAGATCAGACTTTCTTCACAGACAATTATATTGTTTGAAAAGAAATATCTGTGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCTGAAGATCCTGAGTTCAAATCCCAGCAACCACATAGTGGCTCACAACCACCGGCGAGTCTGAAGACAGTTACAGTGTTCTTATGTATAATAATAAATAAATCTTTGGGCCAGAGCGAGCAGGGCCAATGGACCTGAGAGGACGGGGTTGACCGGAGCAAGCAGAGGTCCTAAAAATTCAATTCCCAACAACCACATGAAGGCTCACAACCATCTGTACAGCTACAGTGTACTCATATACATAAAATAAATAAATCTTAAAAAAAGAAAAAAGAAAAGAAATATCTGGAGCTGGAGTGACAGCTGAGAGCTGAAGAACATTTGCTGCTCTTTCAGAGGACCAGAATTTAGTCCCTGACACCCACGTTGGGCTACTAAGAAATATTCGTAAATTTAGATTAAGAGGATCTTGGCCCCTTTCTGGCCTCTATGAGCACCCGTGCGCACACACACACACACACACACACACACACACACACACACACACACACAAGTTTTTCTCTTTGTTTTTACAGAAGCTAGGTATGGTGGCATGCACCTTTAATCTCAGCCCTGAAGAGAGAGGCAGGTGGATCTCTATGAATCTGAGGCCAGCCTGGTCTACATAAAGACCCATACTGAGCCAAGCGGTGGTGGTGCATGCCTTTATTTCTAGCACCCTAGAAGCAGTGGCAGGCAGATCTCTGAGTTTGAGGCCAGCCTAATCTACAGAGTAAGTTCCAGAACAGCTAGAGATACACAGAGAAACTCTGAAAAAAAAAATCTAAGAACTAAAAAAAAAAATTCTGAATCCTGAATAAACTTTGGTATTTGGCATATATTGATTTATTCAAGATGTCTTCCAGGTGTATTGGCTCACAGTTATAATCCAGCCTTTAGAGGCAGAGCCAGGAGGATCACCACAAATGCAAGGCTAGCTGTTTGCATAGCTTGCTCCAGGCCAGCCTAGACTACAGAGTAAGACCCTAACTCAAAAATGAAGCAAACAGCTGGCCATGGCAGTACACTCTAATCCCAGCACTTAGGAGGTAGAAGTAGGAGAATAATCTCTGTGAGTTCAAGGCCAGCCTGGCCCACATAGTAAGTTCCAGGACAGTCAGGGCTATACAGAGAGATCCTGTCTCAAAAGAAAAAAAGATAAACCAAGCAAAATATATTACCCTACTGGGTATGGTGGCCCTACTGTATATCTTAGCACTTAGCAGGTAGCAACAAGAGAATTAGGAGTTCAAGGCCAGCTAACTATAATTTTATAATTTCAGCCTCCACAGATACCAGCACATACCCACATGCAGACACACATACCTACACATAATTAAAAATAGTTTTGAAAAAAAATTTTTAAATGGTTTGGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCAGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTAACAAGATCTGATTCCCTCTTCTGGAGTGTCTGAGTACAGCTACAGTGTACTTGCATATAATCAATAAATAAATCTTAAAAAAAAAAAAAAAGAGTATTTAAAAAAAAAAAATGGTTTGGGGCTAGAGAGATGGCAGAGTGGTTAAAAGCACTTGTTGCTCTTGTAGAGGACCTGAGTTCAATTCCCAGCACACATATGACAACTCACAAGCATCTACAACATCGTTTCCAAGAGATCTAATGCTGTCTTCTGCCCTCCATGGGTGGCTGCATGCACATATGAACTTCAAGCACACACACATATCCACAGGAAAACACACAAGCAGATGGGTGGATGGATGGATAATAAATAAATAAATAAATAAATAAATAAATAAATAAATACTTGTTTTTTTCAGATAAGCTCATGGATGGATAATAAATAAATAAATAAATAAATACTTGTTTTTTCAGATATGCTCAGGCTGTCCTAGACCTTGCTGTGTAGGCAAGACTGGCCTCAGGCATACAGAGATCTGCCTTCTGAGTACCGGGACTAACTGAGTGCACCACCATACCCAGCCCTGAAATGAATAATAGTTTTTAATGGAGCTGGGGTATATGATTCAGTATTAATATACTTGCCTAGCATACATGAGGTCCTAGATTCAATCCTCGGTATTGCAAAAGGAAAAGATATTTTGTATGTATTCATAGGCTGTTTGCGAGCATTTAACTGAAGATTTTAAAAAATTTGTATTAGTATTTATATGTGTGTCAGAGAGGAAGGCGGAGTTCCAGGCAGTCGTGAGCTGCCTGACACAGGAGCTGAGAATTGAGCTCCGTTCCTCTGCAAGGACAAAATGTACCCCTAACCACCGAACTATTTCCTCAGTACTGAAGACATTTTAGAAGACAAATGTCTGTTAGAGACACTGACCTCAATTTTCTACATAATAACCATGGCAGACTTACAGATCAGGTTATGTTGACCTAAGACAGTGAGTGGAGAAGTTATCCTCTGTCCTTTATTTAAAAAAAAAAAAAAAAAAAAAAAGCTGGATGTGGTGGCGCACATCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTTCAGGACAGCCAGGGATATACAAAGAAACCCTGTCTCAAAAAAAAAAAAAAAGTTTGTGTAGTGTATTGTTTCTTACAAAAATATTTGTTAGGATTTACCACTGAAGCCTTCTGACTGCAAGATTTCCTTTGGGGGTGGGGAGCAGCGTTGTTTTTTATTTTTGTTTTTTGGTCTTTTCAAGACTGGGTTTTTCTGTAGCATTGGCTTGTCCTGCCTCTGCCCACTGAGTGCTGGGATTAAAGGTGGTTAAGGGTGTACACCACCATGGCTGGCCTACAAGACCCCCTTTGTAGAGAAGTTTAATTCTCATGTCTGTGTGATTCTTTAGGGTTTTTGAAAATCAGCAGGTCTTTTTGTTTGCTTGTGTGTTTGGTTGGTTGGTGTTTGATTATTGTTGTAGTTGTTTACTTCCTGGCTGTTGTTGTTTTGTTTTTGTGGCAGGGTCTTTCAGGTTAGTCCTAGCAAGCCTAGAACGTGCTATATAGACCAGGCTGGCTTTGAATTGGACTGATCCTCCTGCTCCCACATGAGTCTGCCTCCCAGAGAGCTGTGATTGGAGGAAGACATCACCTACTGTGTCCTGCTCAGACGTCACTGTTGCCTCTATTCACCCTTGCTAAGGGGGTAACTTTGGACTTTATTTAATTTTCTGTGTTTATGGTTTACTAGCTTGTTAAGGTAAAGGCTGAGATAATTGATCTTGAGTAGTTTTCTTCCTTTATCAATTTTTAAAATGTAATGTATGAGCGCTCTGCTGCATATACATCCGTAGGTGGCCAAAAGAGAGCATCCGATCCCTTATAGATGGTTATGAGCCACCATATGGGTGCTGGGACTTGAACTAAAGACCTCTGGAAGAGCAGAGCCATCTGATTTACTCATCTTAACCGCTGAGCCATCTCCCCACCCCCAAGTGCCTGTTTTCATTTTGTTCTGTTTTGTTAAGCTCCAAATATGTTTTAATCTTCTTACATTTTCTTTGTGACTTCATTTTTGGTTTTGTTTTGTTCCGTTCTTCATTTCTTTGATTTTTGAGACAAGGTCTTACTATGTATCTCTGGCTGACCTGGAACTCATAGATCAGGCTGTCCTGGAATTCAGACAGACCCACTTCCCTCAGCCTTCCGAGTACTGGACTAAAGGCATGAGGCGCTATCATTTGTCTAGGCTATGACTTCATTTTTGATCCATAAGTCATGTTTTCCATATGCCATTCACCCATCCATCGTTTATTCACGTTTCTGAGGTTTCACTCTCTGGCCCAGCCTGGCCTGCACCGCCTGGCCTCCCTGCTTCCATTTCCCAAGCGGAGGGATCACAAGCATTCATCACCATGCCCAGCTTTGAGAATTCTTTAAAGTTTTTATTTATTTGTTTGACTGTTTTTGAGACAGGGCTCCATAGAGCCCAGACTAGCTAACCTTGAACACTGTGTAGATGAAGTGAACCTTCAACTCCAGGTTCTTCTGTCTGGTATCTTGCCTTGTTGAGATTACAGCCCTGCTCTACCACACCCTGCTGAAGTTTTTTGTATTGATCTCTGCTCTAATTCCACCAAGGTCAAAAACCTCTGTGCTTTCAGTCTTGTGGCCTTTCCTGAGACTCCTTTGGTCGCCTAGCAGCATGACTTTGTTTGGTTAGTTGTGAGGTGTTTCGTTTTAGTGTTTACCCTGCCCGCTACAAATGGAAGACTCGTCTCCCATCCCACTGCGTTTCTGCCTACTCATTGTTGAACCTGTTTGCAGCCGGCAAGCTGGCCTAACTCTTGCATCTGTGCAGGTTACCTACAGTAGTTTTGCTAGGGGTAGATTCATACAAATGCTTTTCAAGGTGTCACTAATTTGTCAGTAAAGAGGCTGGGCAGGAGCTAGATGAGAGCACTGCTTCTCTGTCTAAACACACAAGTGTAGCTCACGGGGACTTTGGGCAATGGATAGGAGAATGTGCAAAACTCAATAGTCATTTCATTCCTGAAACAATAGTCTCCTAAGAGTGTCAGTTTGCATATTTTTTATTCAGTCAGGCTTCCTGCATTTCACTTTGTTAAAAAGTTACATTAGTGAGGCTGGAGAGATTGGTTAAGAGTTTTGTTGCTTTTACAAAGAACTCAAGTTTGTAAAGTTACACCAGGGACTGGAAAGATGGCTCAGAGTTAAGAGTGCTTGCGCTTACAGAAGATTTGGGTTCGATTCCAAACACCCACATGGTGGTTCCTCTAGTTCCAGGGGATCGGATGCCCTAGTCTGACCTCCATGGGCACCAGGTACTGGTACACACACACGTATGAAGATTAAAATATATATATATATATATATATATATGTCAATCACACCAAACAAGGGTGTTGTGTCACATGCCTGTAATCCCAGCAGCACTTGGAAGACAGAGATAAGAGGATCACTACAAGTTCAAAGCCAGCCTGGGCCACTTGTCAAATTCCAAGCTAGCCAGAGCTACATAGCAACACCCTCTCAAAACAAACAAACAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAAAGGTCCTGAGTTCAATTCCCAGCATCCACATGGTGGCTCACAACCATCCGTAACGAGATCTGGGTGCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATAAATAAATAAATCTTTTTTTAAAAAGTGTTTAAACAAACAAACAAACAAGAAGAGATTATTTCTGGTTTGAAACCAGATTTGTTTTTCAGTTTTTTCTTGATAAAGGTCCATTCACTCTGCTTAAAAAAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAAAAGAAAAAGAAAGAAAGAAAGGCCTGGCTTAGTGGCATGGCACACCTTTAATCCCAGCAGAGACAGGTGGATCCCTGAGTTCAATGCCTCATCTACAGACCAAGTTCTAGAACAGCCAGAGCTACATAGTCTTCAAAACCAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAGTATAAAATACCTGAGCCATGTGTTGGTTACGTACTCTTTTAATCCCAGCACTTGGGAGGCAGAAGCAGGTGGATCTCTGAGTTTAAGGTCAACCTAGTTTACAGAGTGAATTCCAGGACAGCCAGAGATACACAGAGAACCCTTTCTCAATAAATAAATAAATAAGCAAGCAACTAAACAGATATATAAATGAATAGACTAGATTAAGTGGCTGGGTGTGTACCCACTTGCCTCTCATTCCAACACTTGGGAGGCAGAGGTAGATGGGTCTCTGAGTTCTAGACTAGCCAGGGCAACAGTGAGACCCTGTCTCAAAATAAATAAATAAAATAATAAAATATAAAAATAAAAAAGTTACTTATAACCCAGTGTGGTGGCACACACTTGTAATCTCGGCACCAAGGAAGTTTGGACAAGGAGTGAGTTGTGAATTCAAGGACCTGGGCCATATAGTGAGACACTGTCTCAAAATCCTACTCCAGGGCTGGAGGAATGGCTCAGCAGTTAAGAGTACTTACTATCTGCTCCTCCAGAAGTCCAGAATTCAATTTCCAGCAACCACATGGTGGCTAACAATCGTCTATAATGGGATCTGATGGCCTCTTCTGTCTTGCGGGCATACATGCAAATACAGCACTCATGTACATAAATAAATCTTTTCTTTTAAGCTGTACTAAAAACTTGCTTATTCTTCTGTAGCCTTGGTGCTCCTTTCCTTCAGTTATTTTGTTAGGTGTTTCCTTTTTATCTTGGTTTGGTTGTTCGGGGGAGGTTGTTTTCAAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGCACTTGCTCTGTAGACCAGGCTGGCCTCTAATTTGCAGAGATTCGCCTGCCTCTGACTCCTGCGTGCTGGGATTAAAAGGGTTCACTACCACGGCTCAACTGCATGCTGAACTCTTTATAAATATAAACAAAACTAGCCGGGGGCTGGAGAGATGGCTTTTACAGACATCCCAAGTTTGGTTCCCAACACCCACATCAGGCAGTTCATATCTCCCTCTACCTCTGGCTCCAGGGGTGCTAATACCCTCCTCTGGTCTCCCCTGACACCAGGCACAAACATGTTATACACATACATACATGCAAGCAAAAACAATCATACATAAAATAAAAATAAAGGAAATAAAAAGAAAACAAGCCCAAATCAAGGAGAGTGAAACCCCAGGAAGCTAAGGTAGAAGGATTACTGTAAGTCTGAGGACATTCTAAGCTACATAGTGAATTATAGGCCAGCCTGGCTACAGAGTGGAACCTCCCTTCAGAAAAGGGGCGAGGGTCTTGGGGTGCAGCTCAGTGTGTACAGTATTTACCTAATATGCACAAAGCCTTGGGTTTGATCCTCAGTACCACAGACCCATACAAATATGGCAGCATAAGCCTGCAATCACATCACTTGGGAGAATCAGGAATACAAGGTCATCTTCAGCTGCATAGCAAGTCTCAGTTGAGACCAGCCTGCGTTAAATGTTTCTCATATATATATGAAACAGGGTGTCAGTGGGTGGCCTGAAACTTGCTATGTGAATCAGGCTGGCCTCAGCATCACAGAGATGTATCTGTCTGCCTCTGCCTCCTGAGTGCTGGGATTAAAAGCATGTACTACCATATCCAGCCAAGTCTGGAAAATGGTTTAATTTTATTATTTCACATGTATGGGTGTTTTACCTGCATGTATGTGTCTGTATGCTACATGTATTACTTGTGGCTATAGAGGTCTGAAGAGAGCATCAGGTCCCCTGGAACTGGAGTTACAGAGGATTGTGAGCAGCCATGTGGGTTCTGGGAACTGAACGCTGATCTGAAAGAACAGCTACTGGGTTTTTTTGGGGGGGGGTTGTTTTGTTTTGTTTTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACGCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCAACACCACTACCCGGCCAGCTACTGTTCTTAACCACTGACCCATCTCCCCTGTCCCAGTCTAAAAGTTTTTGAGGACTTTAGTCAGCAATCATAAGCCACAAAGGCACACACCAGAGCAGAAAGATGACTTCTCCCGACTAAGGTGTAGCTGTGGCCCTTCAGGATCCCCAGTTGGCTGAGGCTCCCAGACTGTCCCCTCTTGTGCCCAGGGTTTAAGTAGTAGAAGAGCTGTTCCTGGCAGTGACCTCTGACCTCTCCCTTAGCACCTGGCCACATTCATCATGGACAAGAGTGAGGCTATTGCCTCCGTGGATGACGCCATCCGGAAGCTGGTACAGCTGAGCTCCAAGGAGAAGGTCTGGGCCCAGGAAGTCCTGCTGCAGGTGAACGACAAGTCACTGCGACTGCTGGATGTTGAGTCCCAGGTACTGGTTGACAACAGCAGGTACAGGGAGCTGACGGACAGAGGCAGGGCCAGATGTCCAGTTCATCCAGATGTGTGTGGCATGCTGGTCTGTGTAAGGGAATTGAGCCGA
D3_15 141209927 141224925 14999 CAAAACCAGCAGAAGGGAGAACCAAAGAAAAAGTACAAAAAAGTATGAAAAATTCCATAAAAACACAAAATTGGAAACCATTTTACATAAGCAGAGAACTGTGATGTAAAAGCAATGCCCAACGCATCGCCTGACAGAAACCTCAAAAAAAAAACAAACAAAAAAAAAAAAACACTGAGCTTGATTTGCGCTAGCCACCGATTTTCTTCTTAGCTTTGTCTCTTTAATGATTTATTTGTATTTTATGTGTATTGGTGTTTTGCTTGCATGCATGAGGGTATAGGATCCCCTGGAACTGGAGTTATAGACATTTGTGAACTTCCATGCGGGTGCTGGGAATTGAACCTGGGTCCTCTGGAAGAACAGCTAGGGTTTTTAACCATTGAGCTATCTCTCCAGCCCCTCTTCTTAGCTTTGTAACATATAATTGAAGGAAACTTTTCAAGTCACATCTGCTTTCAAAAGTCTTAATCTATATCGGTCCTTTTTCCCTTCCTTCCTTCCTTCCTTCCTTCCTTTCTTTCTTTCTTTCTTCCTTTCTTTCTTTCCTTTCTTTCTTTTTTTCTTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTCTGGCTGTCCTTCCTGGAACTCACTCTGTAGACCATGCTGGCCTTGAACTCAGAGATCTGCCTGCCTCTGCCTTCTGAGTGCTGGTTGCCTGGCTACATTACTTCTTTCTATACTTTCTTGTTTCATAATCAATGTTAGAAAGGGACAGAGATTGGATGTGGAAGCCCACACCTTTGGTTCCAGGACTCAGGAGGCAGAGGCAGACAGATCTCTGTGAGTCTCAGGCAGTCAATGGCTGCATAGTGAGACGATGTCAAATTTTTAATTAATTAAAATCAAGGTTAGAAACTCACTGAAGGAAAAAAGGAAAGAAAGAAACAAACACCACCAGCGACTCCTCCAAGCACCACCCACTATGGTGCTGTTGGGGATTCAGAGCAAAGGGCTCTGATTATCCCCCACACACACACTAAGCATCCCACACTCCCCGTGAGTTAAACAGAAGCCCAAGACATGGAGGTATCCCTCTTACCTCCCCCTCCTCTGGTTCTTACTCCTACCCAGGGTCTACCTGGTGGTCCTACGGCTCCTTTTCAAAGTGAACCCCTCTCCTTGGGCTTCTTTAGCTTGCTATCCAGTGTCCCTACTGGGATCATATGTATGGGACATTCTCAGCCTATCAATACACTGGGCACAGACCTGGATCCCCCACCCCTCCTCCCTCTCCAGTGCCACACTGACCAGGAACATGACCTGGCTAGGGGGGAAACCATTAGCCAGAGCTCACCCCTGCTCCAGAGATGCAAGGGGACTTCCAGAAAGGACACATAATATCTCTAAGGGGCTGTAACTGAAAATCTCCTAGCCCTAGCCCTGTGGTCAACTGGGAACATGTGACCCTGTCACTCCCTAGGCACACCTCTTGCTCTAGTCCCTCTGAGGAGCAGGGCAAGAGACTCCCCAAGATAGTTTGAGCTGGGCCAGCCTCTTGGAGTCTCGTCTCCCCCCCCCCCCCCCCCCCCCCCCGCCTGCTGGAGTGTCACAGTTTCCACTCCCGGCTGAGAAGCACAGCCTCTCTTCACTGCAACCTGGGCAGCAGAACAGGAGGCAGCTGCAGCTGGGAAGCAGGAAGAGACTTCCGTCCAGCCCTGGGTCTCAACTCTTCCTGGTCCTGCAGGGGCTACCAGCCAACACCAGCCATGACCAAGTACGTCAAGATCCTCTATGATTTCACAGCGCGAAACGCCAATGAGCTATCTGTGCTCAAGGACGAGGTCCTAGAGGTAAGGACCGGGGCTATACAGGGGAAGTGGGGCGTGTTCTGGGACCATGGTCTGTAATCCACCCATCTTCCCAGTGTGCTCGACCTGGCTTCCTACTGGGCCTCCAGTGGCCTCTCTGACCCCCACCACAGTCTGGCTGAGCCACTGAGCTGCCTTCTGATGTCTGGGGGATCCCTGGAGGTCTACCGTGGTGGGTGGCCCTTCGGTAGAAGCCAGTAGGGGCTTGTCATTCATGGCCACCCAAGATCACCACCCTGGACCAACTTATTTGTGCCTAACCTGAATCCAGGCCACCTACTGAGGGACAGAAAGTGGAGCAGGCTGGGGGAAGCTCTGCATTTAGAATCAGGTTAAGAGGTACCCTGTCTCCAGGACTGGGGAGGCCAGGCAGGCACTGGGTAGCCCATCAGGCCAAGACATGTCCAGAGGATGGAGAGCTGTGCTTGAGATACCTGGACAATGCTTTGGCCCTCTGTGAAGGGTTTGGCAAGTGTCCCCAGCATCTGACTCTAATAGTACTTGAGGTCTGCACGAGGATGTTGGTGAGTCTCACAGACATCTGGACACTTGGCAGGTGCTGGAGGACGGCCGGCAGTGGTGGAAGCTACGAAATCGCAGTGGCCAGGCGGGCTATGTGCCCTGCAACATCCTGGCTGAAGCTCGGCAGGAGGACGTGGGTGCCCCCTTGGAACAGGTGATCGCCTGCCACGGCTCCATTCTGTCTACAACACAGTCAGAGCCCCTAAGGTCAGGGGTGCAGCTGGTCTCAATGTGATATTTGAACCTGATCCCCGGAAACCTGCTGCCCCCAAGCTGGGCCAAGGGCTGTGTGGGTTCCTGGGGTCTTACTCAAGGGAAGAAAGGGCTGGCCCATCCATCTGGGGTCCTGGGTAAGTCCTTATATAATAGCCCCCCCCTCACCTTACCCCTCACCTCTTCCTTACTCCTTGCAGTCTGGCCAGAAATACTGGGGTCCAGCCAGCCCCACCCACAAACTGCCCCCAATTTTCGCAGGGAACAAAGAAGGTAAGTAGATCCACTTTGGGGTTAGAAGTGTAGGGAGGGGCAGGGGAGGGCGCTGATAGGATGCGGAGGGTGGGGAAGAGGCAGAGAACGGGTCGAGTGAAGGGGCTGAGAGCGTGCGCCCCACAGAGCTAATCCACCACATGGACGAGGTCAATGACGAGCTCATGAAAAAGATCAGCCACATCAAGACACAGCCGCAGCGCAACTTCCGCGTGGAGCGCAGCCAGCCAGTGCACCTGCCGCTTACCTTCGAGTCAGGTCCGGATGAGGTCCGCGCCTGGCTGGAAGCCAAGGCCTTCAGTGCCAGGTGAGTCCCGTGTGTACAGCCGGTGGGGCGGAGCCTGGAGGACAGTGTCCCGCCCACGGCCCCGCCCTCACACCCACCTGCCCCTAGGATCGTGGAGAACCTGGGCATCTTAACTGGACCCCAGCTCTTCTCCCTCAACAAGGAGGAACTGAAAAAAGTGTGCGGGGAAGAGGGCAGTCGCGTGTACAGCCAGCTCACGGTTCAGAAGGCCTTCCTGGAGGTGAGCAGCTCCTGCCTGCTTCTGAGTTCCTTCCTAGGCACTTGCACCTGGGGTCACTGGAAAAACAGGAAACAGCAGGGCCCTGTCCTGTACCCTCTTTATTCCCCAACCTCCCGGCTGAGGCCCAATCTCTTTTTCCTAGAAACAACAAAGTGGGTCAGAACTGGAGAAGCTCATGAGCAAGATCAGGAGGGCCGAGGACAGCTATACCAGCCAACACACTTCACCAGAGTCAGAGGGGGCGCCGCACCTGTAATGGGCCCAACCCAAATGCATGGAGTATTATTTTTATATGTGTATTATTTTATACCAAGGACATAGATGGGCTGGGTGTAACTGGCTTACCTGCCCTCCAGGCAGAGTCCTGTGTATGAGCCTAGCTACACCTGTGGGTAGCTCTTTTACCACCCGAGCCCTACCCCCAACCCAATACTCTTCTTCCAAAACCAAGGCCAGCCTCAGCCAAACCTGTACCTGGCAGTGCCAGTCCCTTCTCAACCTGCTGCCAAGGCCCCAGGACTCTCCCGGTGGGCAAGGCCTCTTCCCCTGGTCTTCTTGGTGCACATGAAGGTCCTTGTTCCTTACAATGCTGGGCCCCGACTCTGCAGCTCTCCCTTGTGTTGCTTGTTTCTACATTCACGTTTTCCTGCATCCGCTGTACCCCAATTGCAGGATGGGCCTGAGCCCTCAGCTGACCAGCCTGGTGTCCTTGCCTACATATTTGCCCACTCCCTTCACGCTGCTAGCATCCCAGGAACAACCTGGTCAGGGGCTCCAGGACAGGAGGCCTTGGGTGACCTGCTGGTATAGAGTATGCTGTATTCTTTCTTCTTTGAGACCTAAGAATGAACCAGACATAGGTTCTGACTTCAAATGTCCCATCCCACCCAAAGAGCCTGTGATAACAGATGTCTCTGGCTATTTCCACCAGCCTGTTCGCTGGTGTCTGGTGCGTTTCCCAGCTCTGGATGGAGCCCCAGACCCAGAGATGAGAGTGGGGACTCCACAGCTATGTCAATAAACCAGATGTCTCCTAGCAACTTAGATATCTGTTTCTTGGGGTAGAAGTGCTCTGACACCCAAACTGCCTCTTCTTCCTGCTCCTTTTCTCTGGGATAGTGCATTGGCCCTGAACTCTGAATCGTCATCCTGCCTAAGCCTTCTGTGCTGGGATCCCACTTGTAGACCACCAAGCCTGGTCATGCCCTTCAAAGATCTTTCCAGTAGAGAGCAACCCACCCACACAGGTAGTGCTTTGGCCATGCCAGGGGACACCTCTCACCTCCACACCCATCCAAAATGGCCCTAAGGACATTTCTGGGAGGGACTCCAGAGGGTGTCTAGACAGCCCAAAACCTTCCAACCGGGTGGCTTTCAAAGGTCCCATTCTGAGTAGGAGCAGAGTTCAGCTGGCCCAGGAGCATGAATCAGAGGGAGATAGAGGCACTATTTAGAAGACTGTTCCAAGATGAGGGAGGTGTCAAAAAGAGACCTGGGGCCTGAAAGACCTCACTGACAAGTTGGAGAGAGACCCAGGTATCTGCCTGACTGGCCAGAGACTTTCCTTTGATGCAAGCCTTTGGTGCCATCTGCTGGTAGCTCCTTATACATCTTTGGGTGACTGTTAGATACCTTGTATCTGGTGTGAGGACTGTCCTGCATTCCAAATTGCTAGTGAGTCAAATCCTGACAAGGATCACCTGAATCCTCAACGTACTGTGCCGAGACTGAGGTGAAGAGACTGTGCCCCTCAACTAATCCACCACCTACCCAGACTTGGCCTCAGGTCTCAGAGGCTCCAGCCCCTCAGCTCAGACAAGCCCTACAGGACCCAGTCTTTCCAGCAGTCTTCTCTCCAAGATGGTTGGCGCCACCCTTAGGGAGTCTCCAGAACCTGTATCTGCTTCATCTAACCCTCCTAGCCCTTCCCCATCTGCCTACACTTACTGTGGCTTCCACAGAGCTGAGGCGGCTCCAGACCAAGCAGCCTCCCCTGCCCAGGAGCTCTGGACTCCTCACTCCCATGTGCTTGGGGCCCCAGCTGAACAACTGGCTCAGGCCTCTGAGTTCTTGGAAGTTGTCCCTTCTTCTCTCAAGGGCCTTCTACCACCTCTAGGTCCTTTTTAAAAAGTAGAGAGCTGGGTGTGGTAACATACACATTTAATTACAGAAAGCAGAAAGCAGAGGCAGGAGGGAGATCTATGTGAATTAGAAGTAGGTCGACACAGCAAGTTCCAGTCCAGCCAGGGTTACAGAGTTAAAAAAAACAAAAACAAACAAACAAACAAACAAACAAACAAGAAAAAAACTGTCAAAAATCTTTACTTTTAAATATTTTTTAGATTTTCTTGCTTTTACTTTATGTGTATGAATGTTTGCCTGTACATGTCCTACATGTATGTACACCATATACACCACATGTGTGCCGTGCCCACAAAGGCCAGACAGATTCTCTGGAACTGGAGTTATAGATAGTTGTGGGTCCTGGAACCCCAACCCTGGTCCTCTGCAGCAATAACCATCCCTAAAGGCTGAGACAGCTTTCCTTATTAATTCTGTGAGGCACAATCTCACATAGCACACGCCTCATACTTTTGATGTGGCTGATGGATGGCCTCGAAGGCTTCTAGGCAGGCACCCAGTTTAATGTGTTGCTGGGGATAGAGGGGAAACATTCTGCCAACAGAGCTAAGTCCTGAACCCTCAGAACCTTTCAAAGAGAATAGCAGAGGACCACCCATCACAGGCACCTAGTGAAGAGATGGAAGTGTTACAGCCCCAGGGCGAGTTAGCTTGGGACCTTCCCTAACTACTTACTCTTCCCTCTTTGGATGTCACTATCCGGGGCCTGCAAATGAGAGATAACAAACAAGAACCATGCAGCAAGCACTATCTCTAGCATTGGGTGACCTCACTCTGGTCTAGACTAAAGAATGAGACACAAAGAACCCAGGTCACCTAGGCTGGGGTTCAAGTGCAACTTTCCTAATTATAATACAACTCTCCCTGATCCCTACCCAGCAAGTCAGCAGGAATGGGAAAGGTTTGCCCTACCTGTGGGAACCCAGCTGAAGAGGGTCTGTCCATAGGTTGGGTAGATTTGCCCTGGACAAGAACGAAGAATGAACTTAAAACCCCCAAACCTAGAGTTGGTTTCTAAGCTACTTAGCCAATAAGCTCTTCGTGTGTGGGACTGCTGTGACTACTGCTCTGTGCCTAGCTCCACCACCAACAAATATTTCCATGAACAAGGCCCATCTCTGTCTGGGGACAAGCATGTCTTTGTGATCTGCCAACCTCCTGACCACCCACAGCAGGCCATTCAAACACAGGGCTTCCTATACCACTCAGAAGAGGCTTTCCCGTCTTTCTTAGAAAAAGATATAAAATAGTTGACTTCTCAAGGTAATTGGAGGAATGACAGAGAAGCTGAGATGTCCCTTCTACCTGGGTGACCCAGCACAAGATGCACACTTAACCTGGGGACAGTGATGCTTAGCCAAAGTGGCTAAATCTCTGTGCAAATCCCATGAAGGGACCCTGGACATGGGTGAATGCCGAGAAAATGCTTTTTGTGTGGAGAAGCAGAAAGGATCTACCCTGGCCCTGAATGTTCCCCTGGGAGTATTGAATGCAACATGACAGGCAAAACTGTCAAGTGTCAGAGAGAAAGCATGGAGAAGATCTGTAGCCAAATAACTTGTGCTCAAACAAGAAGACGTGAATTTGAGTTCAAAGAGACAATGTGTGTGTGCTGCTATGGGGCATGGAGCAGAGAGGAGTGATGGCCAAGCTATACAGCTCTGAGCATACAAAGCCTAAGAGTCGCTTGCCAGGCATGGTGATTCGTGCCTGTCATGTTATCGTTTGGAATGCTGAAGCAGGAGGATTACCATGAGTTTGAGGCCAGAGCAGGCTTATAGTGCTACCCTGCCCCAAAGAACAACCAAAACTTCTTTTGTTGATCACCACTCCCCAAAGTGCTGTCAAGAGACACATTGCACACTGAGTGGTCTTAAGTGCACACCTCACTGTGGATGGTATTAGGGGCACAGCTGCTTAAGAACTTTCTTCAGGGTTTGAGGTACAGGCGAGTAGCAGGGGAACAGGGAGAAGCACGGGGTCGAGTTCAAGGAGGATCTTTGGATACTTGGTTAGGACTCAGCATCCCTAAGGTGAGTTTCAAAACTCAGAGAGCCCTGCTAGGCTTTCCACCTACAGGTCCTGAGCAAGAACTTTCTGAGGGGTCAGAGAGATGGTTCAGTGGTTAAGAGAACTGGTCTCTCCTCTAAAGAAGCCGGGTTCAGTTTCCAGCACCCTTGCGGCAGCTCACAACTGCCTTAAAATTAAATAATATAATATACAAAAATCTTTCTGTAACTTCCCAAATAAGGCATTTGAAACCTCATCTTCCTGCTTTGAGTGCAACAGTGGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACATGCTTACATGCCTGAGTGCAAGAAGAGTCAGAGACCAGAGGTCAACACTGATTTCTTCTCTCTCTACATTGGGAAGAGCGGGGGGGGGGGGGGGTAGCAGTGGAGATTGAACCTAGAACCTTCTATATGCTAAGTGTCCTGCTTAGTCTTTTTGTCAGCTTTACTTGAACTAGATTCATTTGGGAAGAGAAAACACTAATTGTGGAAGTGGTCCACCAGATTTGCATGTGGACAAGCCAGGCAGTGGTGGTGGCACATGCCTTTATTCCCAGCACTGGAGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGACCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGACAGGGCTACACAGAGAAAGCCTGTCTTGAAAACAAACAAACAAACAAACAAACAAACAAACAAATAGAAATATTTGCCTGTGGGCAAGCCTGTGAGACATTTTCTTAATTAATGATGGATGTGGGAGGTGCCGTCTCTGTACACATGGTTCTGAGTTGTATAAGAAAGCAGGCTCATAGCATTTGGAATACAGGTCTCTCTTAGCTTGAGGCTAGCCTGCTCTACACAGAGAGTTCCAGGACAGCCAGGATTGTATAGACAGATCAGATCTGTCTAAACTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAGAGAGATGGCTCAGTGGTTAAGAGCATTTGTTACTCTTACAGAGGACCCAGGTTTGATTTCCAGTACCCAGAAGATGGCTTTCAACTACCTGCAACCCCAGTTTCAAGGAGTGCATCAGACATAGACATGGTGCACATATATGCATGTAGGCAAAATATTCACATATAAAATAAAACAAATCTTTTAAAATGAAGTAAAAAAAATCTTTTTGAAAGACAGAAAGCAGGCTGAGCAAGGCATGAGGAGTCAGTCATTAAGTAGCACCCCTCCATGGTGGCTTTTTCTTTAGTTCCTCCTTAGTGTGGTGGTTTGAATAAAAATGGCCCCCGTAGGTCCATAGGGAGTGACACTAACAGGAGGTGTAGCTTTGTTGGAGGAAGTGCGTCACTAGGAGGTGGGCTTTGAGGTCGCAGATGCTCAAGCCTGGCCAGTGTGTCACTGTCACTTCCTGCTGCCTGTGGAATTCAGAAGTAGAACTCTCGGCTCCTTCTCCAGCACCTGCATGCCACCATGCTTCCCACCATGACCATAACTGACTAAACCTCTGAGACTGTAAGCCAGCCCCAATTAAATGTTTTCCATTATAAGAGTTGTTGTGGTTATGGTGTCTCTTCACAGCAATAAAACTAAGACACTTAGCTACCCTGATCATGGATTGGAACCTAGTTGCCAAATGAGCCCTTTCCTCCCAAAGTTAGTTTGGGTCATGGTGTTTTATCACAGCAATTAGAAACCTAAGACACTAATCCAGCCCTCTACCACTGCTAGATCCCCAGACCTGCCCACCATATTTTCTGAGATAGTTCTCGCACTAAAACTGGAGCTCATTTCTTTAGCTATCCTGGAGAGTGAGACCTTGGGACCCACCTCTATGCATCCCCAGTTCTGTACTTACAGGTAGGTCATAGCTGCAGATGCCTCACCCAGTTTTTTATGTGGATGCTGAAGATCTGAACTGAACTCAGGTCCTCAATGTTATTGTGAGCTCTGAAAACCAAACCCAAGGCGTCCTGCATGCTAAGTAAGCCTTGTCAACTGAGTGACATCCTCACCTTGGTCCTTATTTTCCTTCTCTTTCTCTTCTCTGTCAAGCCTTCTCATACCTCTCCTCAGGATGAGGGTCCCTGTGCATTCTTACAGGTAGACAACATCTTGGGCTCTCGAGGCTGGATCCCTTCCCACCGCCAGGGCAATCCTTTCATTTTCTGATGCAGTTCATGCCTTATATGACCTCTGAGTTGACCTGTGTGGTAAGGTCCCAGCTATTCATCTAGCAATCACAGTTCAAAGAGACAGTACCTATGCAGGCTGAGCAGGTCATATTGCAGCAACTGTGATCAGAAGTCCTTGGTTCTCCCTCCCTCCTACCTTCCTCCTAAACATAGCTTCCATACTTCCTACAGCCCCAGGCTTTGGCTACACCTCTTCCTCCTAGTAAATAGAAGTTAAGGGAGAGGGCTGGAGGCATGGCTCACTGGGTAAGAACACTGACTGCTCTTTGAGAGTTCCTGACTGAGTTAAATTCCCAGCGACCACATGGTGGCTCACAACCATCTGTAATGGGATCGAATGCCCTCTTCTGGTCTGTCTAAAGACAGCTACAGTATACTCATATACATAAAATAAGTGAATCTTTAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTTGTAGTTAGGAGAGAGCAGTGATGAGGACAAATTCCTACACTCACCCCAAGAGATCAAGTCAAATACTCAAATGGGCCCACTCAGCTAGAGTTCACACTGGGCCTGTGGGGACCTCACTTACAGTACCAGTCCCTCACTTGCAGCACCAGTCAGGCCAGATCTGTGGCAGGAAGAGGAAGATGGTTTTGCACAAAAGAAACCAGAAATCTCCAGTTTCTCTGTACACTCGAAAGGAATGCAACTTCAAAACAACCATTTAATTCTCCCATCTGTTCTACTTCCTCGTTACTGGAGTTTTTAGGGTCAAAGCTGGGCTTGGCCACTGGGCCTAAATATATCAATTAAAGAGACAGTAATAAACCGGGCAGTGGTAGTGCATGCCTTTAATCCCAGCACTTAGGAGGCAGAGGCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAAGGCTACACAGAGAAACCCTGTCTTGAAAAACAAAACAAAACAAACAAACAAACAAATCATAAGTAAAGCAGACCAGTGATCCCTCACCTCCACAGAACTTGGAATGCCCAAAATTCACCTGGAAAGCATACCTGCCCAAGGACCAGCAAAGTTCCTGGAATGCTGGGAGTTGTAGTTCTTGGAAAATAATAACGCCTCATGGGAAAATAGAGTGATCTTTAGGTTTGTTGTCATAAAAAGACCCCTGCAAAACATGTCTTGGGGCCATTGTGCTGGGAAATCCTAGGGAGTGGTTCTGATCATTGTCCATCTCTTGGTCAATATTTAATAAATTTTGCTTTTCAGGTTAGAGAGATAGTTCAGAGGTTAAGAGCACTGTCTGCTCTTCCAAGGGTCCTGAGTTCAATTCCCAGCAACTGCTTGGTGGCTCACAACCATCTATAATCTGGTGCCCTCTTCTGCCCTGTAGGCAGACTGCTGTATACATAATAAATAAGTAAATCTAAAAAAAAAAAGAAAAAAAAAAGTGTGCTTTAAATGTGTCCCAAAATGATGGTTAGCAAACTGACTTGATATTTAGGTTCAAACTGTGGTCTGAGAAATTGTCATAACGCCAGGGCTTCAGAAGTTCACTTTGGTTCCCTTATGCCATGGGGTAGCTAGTCTATATCAACTTGGCACAGGGTAAAGTCATTGAAGAGAAGGGAGCCTCGACTGTAGGCAGGCAATCCTGTAGAGAGCTTTTGTAATTAGTGATTGAAGGGGAGGGTTCCCTTCAGAAACAGGAAGAGCAAACCTTGCAGGTTACGGGTTCTATTTGCAGTTAAGGTGGTTAAGACTAAGTTCCTGTTTGTCCTGTGTTGATTCCTTGCAAGTTAAGGTTTCCATTTGTAAAGAAGAGTAAGTTTCTGTTTCTTCGCTCTGGTGAAACCTGCTTACAACCCCATTCGCAGGAGTAAACCTAGCCTTTGTTCCCTCCCTTTCATTATCTTTCTAAAAAACGTATAACAGCCAACCCTCCTGCCCCTGTGAACCCTCTCTCCCATTAAAGGGACTTCAAGAGGCCAGAAGGGGCTGCTCTCTCAAATCCCAACTTAGGGTAAGACAGCTGGCCAGCCGGCCAGTCCTGAGTGCATCCAAAATGCAACTTGCTTTAACTGGACAACCTTAGATTTGGTCTTTTCTCCTTGTGATTCTCAAGTTTAGCATGGGCCCAGCTTGTGGGTGATGCCACCCGTGGTGGGATGATTGTCCCAGGTTTTATACAAAAGCAAGCTGATTAGACCCTGAGGTCTAATCAAGCCAGTAAGCAGTGCTCCTCCAAGGCCTCTGCATCAGCTGCCTCCTCCAGTTCCCGCCCTGCCTGAGTTCCTTCAGGATGAACAGTGTTGTGGAAGTGTAAGCCAGACAAACCGGCGCCTCCTCAGCTTGCTTTTGGTCATGGTATTTCCTCACAGCAATAGGAACCCTGACTAAGACAGCCCAAAATGGGATTCCTCACAATTCTTGCAAGGTTTTTGGGTGTTTTCTTTTTTTGTGTGTGTGTTTTGTTTTGTTGTATTTGTTTTTTGTTTTGTTGTTTTAAAACAAGGTCTATTGTAACCCTGGTGTGGTGGGATTCTCTAGACAAGGCTGGCCTGGAACTCTGAGATCCACCTGCTTCTGCCTCTTGAGCTGGGACCAATGTCTGTGGTATTAACCCGGCCAGAATTCTTAATTCTTTGGAGCACATTGTTTCAATAATATTTCAAGTGCCTGTACCTCTTATAGTATCTTCTTATATATATTATCTTCTGCCAGGATAATTATTCCCTTCTTTTTTCTACTGGGTCCATCTCAGCCTACTCTGGCTTACAAAAGAAATACTACCTTGTCCCTAGGTATCCTTAGGGCCAGGTGGTGGTAGAGCATGCCTTTAATCTCAGAGGCAGAAGCCTGTAAATCTCTGTGAGTTTAAGGCCAGCCTGGTGTAAAGATTGAGTTCCAGGACTGTCAGGGCTATATAGAGAAACCCTGTCTCAAAAAATAAAACAAAACAAAACCAAAAAAAAAAAAAAACATAAGGCCGGGCAGTGGTGGCTCATGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCGAGCAGATTTCTGAGTTCAAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCTAGGGCTACACAGAGAAACCCTATCTCGGAAAAAAAACAAAAAAACAAAAACACAAAAGCATAAGACCAAGGTAGCCTCAGGGACTTCGCAAACCACATAAGAGCCTGTGCATATATGATTATGTGTGGAGAGGCTTACGGGATGGTGCATGTATAGTAAACATCACGTCATATAACCTGGATTTTTCCAATATGTAGTTATTTATACATATGAATGTCTTGCCTGCATCTATGCTTGTGCATCCTGGTGCTCTTAGAAGTCAGGAGAGGGCATCAGATCCCCTGGGACGGGAGTTACAGTGGTTGGAAGCTGCCATGTAGGTGCTGGAAATCAAAACCTGTTCCTTTGCAACAAGAGCTCTTAGCTGCCGAGCCTCTCTCCAGCCCCCTTACTTCAGTTTTTATTAAGCTTTTATTACAGAGAGCTCACCAGAGATGACAGTTTATAGCCAATTGAATGTCTTTATTGGAGTTGGGTGGGGTGGTGTACCCCTTATAATTCCAGCACTCAGGAGGCAGAGGCATGTGGATCTCTAAGTTCAAGGCCAGAGCGGTCTGGCTTCTTAACATTGACTCAGACTCCTGCACAGGGTTGTTAGTCACAGGGTCTGTTAGTCATGCCCCTGACATGCCGCAGCCCTCTTTAAGGTGGAAGGCACAGAGATAAGTCATTATCCTGAATGCTTCCTCCTGAGTCACTCTGGATTTCAGAAGCCAACTGATTGTTAAAAAAATAAATCCCTAAGATCAGGACTGAGACGGTAGTTTCAGGCAAGGGCAGCCTGAGGGTATAATAAATATAAGGGGAAGTTACTCAGGGTGGCAAGGTTCAACCTGAGACAGACTTTCTGTTCTGCTTGTGTGCTCAGAAGAGCTGACATTTGCTTTTCTCCTAACTCAGCCGATGACTGTGGCTTCTCCCCTGGTCTAGTTTGATTGGCTAGTCTGAACACTGAAAATGAAGGAGGAAGGAGAAAGAGGTCACTCGCTCCAGACCGTCAAATTCCTGGAATAATGTGGAGGGCCTTGTGTAAACAAATGTTTTACAGGATGGAGGGATTAAAACATTTGCATAAAAATCTTACAAGGTGGGCTGGAAAGATGGCTCGGTGGTTAAGAGCACTGGCTGCTCTTCCAGAGGTCCTGGGTTCAATTCCCAGCAACCACATGGTGGCTTACAACCATCTATAATGGGATCTAATTCCTTCTTCTGGTGTGTCTGAAGACAGCATACAGTGTACTCATATATACACTAAATAAATAAACAAGTCTTTAAAAAAATATATAAGGTGGTGGGGCAGGAGGAGGCGATAAAAATATTAGAATTCTATGTCTAAAACGCAAGTTTTACAATATTTGAAAGGAAAGATAGTTTTGGTTTGTTTGTTTTTTGTTTTGTTTTGTTTTGTTTTGTTTTTGGTTTTTCGAGACAGGGTTTCTCTGTGTAGTTCTGGCTGTCCTAGAACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAAATCTGCCTGCCTCTGCCTCAGGAGTGCTGGGATTAAAGGTGTGGCCACCATGCCTGGCAGAAAGATACATGTTTAAGGAGCTGGAAAGAGTCCGGTCCCCCAAGAAATTCCTTTTCTCAAGGTTGGACATTTAGACAAAAGCCACAATCCCCTTAGAGACTTGAAGAAAGTTCCTGCTTGTTAATAAAAGGCGGGGGCTGAGGGAGTAGGGGACTCATTCTCCTTGTCTGGCAAGAGGAACACAAGGCTCTTCTATTAACGTACTACTGTGGTGCCTGTAAACTTGTGAAGGTTCTAGGCTCCCTCAGTCCCTGCTCAGAAGCCTCACAGGATCTCACAAGCCCCCATTCCCATTTAACCCTGGAAATCTTTCCCCCCTAGCTTCTCCTCCTTTCCAGGGAAATTGCCTTAGCACCTTTTCCCTTTTCACCCACGGAGCACCTATTTTGGTCTCTTTACCACACTCATTTTCATTCAGAAAAATCCCTCAGTGGCTAAGAGCCTTTTTCTACTCTTTACCACTTCCTTGTCTCTTTAATTGACCTGTTGAGGATCAGTCTGAGCCCCTTGGTCACTGGGCCTAGCTTGTTAGCGTTGTCAT
GF3 -1 -1 1 CTTACAGATCAGGTTATGTTGACCTAAGAC
GF4 -1 -1 1 CAGATCAGGTTATGTTGACCTAAGAC
EX52 -1 -1 1 GACCCCCAAGAATCGACTATCTGTACGAC
EX32 -1 -1 1 GGAATGTCGTACAGATAGTCGATTC
GR3 -1 -1 1 GCACGGCACACATGTGGTGTATATGGTG
GR4 -1 -1 1 GAGCCGCCTCAGCTCTGTGGAAGC
Comments:
Target: otter_06_09_21 OTTMUSG00000024549 -- 141206965 -- 141209637 OTTMUSE00000321062 -- OTTMUSE00000321110
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM36 assembly: chr: chromosome:NCBIM36:7:1:145134094:1, start 141206964: end 141209637
Found best 129-bac on NCBIM36: bMQ433b03
Found 10 RP24 black6 bacs on NCBIM36
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:7:141201465:141202464_738 G3:7:141214137:141215136_609
ACCEPT (0) gap pair G5:7:141201465:141202464_738 G3:7:141214137:141215136_584
ACCEPT (0) gap pair G5:7:141201465:141202464_738 G3:7:141214137:141215136_754
REJECT (1) gap pair G5:7:141201465:141202464_143 G3:7:141214137:141215136_609
REJECT (1) gap pair G5:7:141201465:141202464_143 G3:7:141214137:141215136_584
REJECT (1) gap pair G5:7:141201465:141202464_143 G3:7:141214137:141215136_754
ACCEPT (0) gap pair G5:7:141201465:141202464_684 G3:7:141214137:141215136_609
ACCEPT (0) gap pair G5:7:141201465:141202464_684 G3:7:141214137:141215136_584
ACCEPT (0) gap pair G5:7:141201465:141202464_684 G3:7:141214137:141215136_754
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted sequence arms between oligos

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