High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 187150
Chr: 13
Strand: -1
Status: Ordered
BACs: RP24-477O1 : RP24-497L17 : RP24-66K21 : RP24-362J2 : RP24-181M8 :
Instance: Plate 131: Well C05: BACs RP24-66K21 RP24-477O1 RP24-497L17 RP24-362J2
Oligos on GRCm38:
Type Start End Length Seq
G5 95448524 95448573 50 ATATGTGTCCTTTCCTCATTGTATACTCTTGATATCCTTGTCCAAGTGGA
U5 95442720 95442769 50 AACAGATCTCAAAGAATAAATAACAACAGCCAACCTGGATATGATGGAGG
U3 95442599 95442648 50 AACTACAGACGACGCTGATTTTATTCATTCGTTTCCACATGAGATCAACT
D5 95441899 95441948 50 TATTACCTAGCCTGGGAAATACAATCAGAAACTGTGGTTTAATTTTCATA
D3 95441736 95441785 50 TTTGTTTACAAAGCTCAGGCACCAAATGATCCCACAAGCTTATCCCGAAT
G3 95437631 95437680 50 TAAGGAATGGAGACAACAGGGTCTGCTTATGTTTAAAGAACTTACTATGA
Oligos:
Type Start End Length Seq
D5_D3 96211741 96211853 113 CTGCAGGATGGCCTGGGAAATTAAAATTGGGTTCCTGAACCCATCGTAATGTCCCGGCAGGGCAGAGCAAGACTTCAGAGGGGAAATAGAAGTGTATGTACCATATGGTAGAA
G5 96218479 96218528 50 ATATGTGTCCTTTCCTCATTGTATACTCTTGATATCCTTGTCCAAGTGGA
U5 96212675 96212724 50 AACAGATCTCAAAGAATAAATAACAACAGCCAACCTGGATATGATGGAGG
U3 96212554 96212603 50 AACTACAGACGACGCTGATTTTATTCATTCGTTTCCACATGAGATCAACT
D5 96211854 96211903 50 TATTACCTAGCCTGGGAAATACAATCAGAAACTGTGGTTTAATTTTCATA
D3 96211691 96211740 50 TTTGTTTACAAAGCTCAGGCACCAAATGATCCCACAAGCTTATCCCGAAT
G3 96207586 96207635 50 TAAGGAATGGAGACAACAGGGTCTGCTTATGTTTAAAGAACTTACTATGA
G5_U5 96212675 96218528 5854 AACAGATCTCAAAGAATAAATAACAACAGCCAACCTGGATATGATGGAGGGTGATTGTAACCCCAGTACCACAGAGGCTAAGGGGGAGCACCGTGAGTTTGGGATACAAAGGGTGATTGTGTCAAAGTGATGGGGGGAGGGGCGTGGGGGGATCCAGAGCAGCAGGTGTCATTGCCAGTGCCTCACTCTTCACATTAATATATTATTATATATTAATTAATATATATATTAGTATATATCATCACCTCAAGCTACTTTTTAAAATATTTCTAAATGCTGAAATCTGGCAGTGCTGCCTGGTTAGCCATGTAAGACTTCCTCTTTGCCTCCTGGGACAAAGGGTCAAAGTCAGTTGACCCGGTTCTCAGAAATCACCGCAGGTCTCCATTTTAATGAGTCCATCATAGCACAATTACCAACTGCCGGGTTGCAAATTATTTCTAACAAGCTTGACAGGTTCACCAGTGGTTTTGGAGATCTAAAATGTGAATGTTTCTTGGAGCGGTAGAACTCTATCCTGTGGCCTCTGGGGGCTGAAATGGGATGTGTGGAGCTGGGAGTTCTCTTCCTCTTCTTGCTTCTCTCAAGCTCTACCTCCTGGCAGCATCCCTCTCCCAACCAGTGCCCCACTACTTCAAAACCACCATCAGCCCGGGCTCCTGGAATTACAAAATGAGTTCAGATCTCAGATCGCAAGCGAACCCTCCTGGAGAGCTCCTGCTAATTATTTTTGCCATGTTTTGTACGCCGCAGATTGCATCATAGCGTCTCACACTGCAAGTTCAAGTGCTAGGAAGTTCCCAGGAGTTGGGGAGAGCACAGAAGGGAAATTAAACCATCGACGTCCAGGGGCGCTAGACCTGCAGCGTTTGGCGCTAGACCCTTCCTAGGAATCCAGCCTCCGGGGCTCTCCCAGGGAGTTTCTGAGACTCGAGCACTTAGAGGTTTAGTTGTCCCCCACATGGACGCGCTTAGGGGAACGTGGGCTGGGGCCGCGAGTAGTGGGGACCCCCTCACCTTCAGGAAATCCCCGCCTTGGCAGTCGATGGAGACCAGGTCATAGTGGATGGTGATGAATTCCTCGGGTTCGCCGATGAAGAAGGCGGCGCAGTGCAGCTGCGGCCGGTCTGCGGTGAAGGTGAACTGGCCAGGGAGGCTCAGCATGTCCAGGCACCCTGGGAGAGAGCATGGAGCCCGGGGTTCCCAGCTGTCTTGGGAAGCTGCTCTCAGCGCGCCCCATTCCCCGGCTCCGCAGCGCGCCCCAACCTGGAGCTCCCAGCTTAGGCTGCACTGGGTTCCCTCGCTCTGGCTTGGAAGGAGCCGCGTGGATGCTGCAACCCAGGGGTCCCAGCCAGCCCCCCCCCCCCCGCCCCGACCAGATATCCTGCAAGCCTGTGTCCTAGGTTCTTAGCCTCCCGCCTCCCGGGCACCCACACACTGGACAGCCCCAACTCACGCAGAGCCCGTCGGTACGGCTGCTCCTCTGCCAGGTCCCGCTTCAGATTGGCACTGAAGAGCAGGAGAGGGTCGTAGACCGCGGCTTCTTGCACCTGGAGAGGAAAGTGGATCGTCTGGAAAAGTTCAGGGGGAGGGAAAGAAAGCGAAGCAGGAGCAGGGAGCCCTTTTTGCAGCTCAGAGAAGCAAATGGGGCAGGGGGCAGTGGGGCATGTCTCCTGAGTTCAGAGTTTATGCGTGAGTCGAAGACTATTTCCACCAGATTAGGGTACCCAGCCCAGAGAAAGGCAGGCAGTTAGGATGCTCAGGGGCACGGAGAAAGCTGTCTAGGTGAACCAGGGAGAAAGGATCAAAAGTTGGTCTGCTGTCCCTGGCTAAATACTCAAGGGATAGAAAGGTGTTAAGCTCGAGAAAGGATGGCTCACCTCTAGGTAGCGGCTCTCTCCCCTTAGAGCTGTCAGGAGGATCAGAATGAAGTGACATTGGAGTTTGAAGTTCGGTGACATGCTGGCCTTGTGTCTGCAGCTGGAGTGCTCTGCTGCGGAGATCTTAGGCGTTTCTTCCCACACCTTCGCCCACACTGAGAGCTCAAAGCAGCGAGGGTCCCTTTGCTGGGTCTCTTTTATAGAGCAGTAGAGGGGAGGCTCTCTGCCCCTTAACTGATCTGAGCTTAGTTACCATGGGTTACCCTGCCTCGGTGACAAAATGCGTGGCAATGACGAGGGTCCAGCTCTCAGCCACAAATATTTCAGCATGGCCCCGCATTATTCATCAAGAGTGACACTTCAGTCACGAGGACCTAGTGACCTGAACGCATAGCCGAAAGAATGAGGCAGAGGCAGAGAGATTCGGGGAGAGCCTTTCACTTGGCCTGTAAAACGTTACATTATATTCATTATACATATCTGGGGAACTTTTGGTTTTATAAAAGCAAATGACAGACATGTCAGACACATATGACCTCCTCATGGGGCCTAGCCATATAACTGTGGATGGGACTCTGATGGGAATGGCCAGAGCGTAGTATCAGTACGGCTATCATTCCCCTCCAGAAGAAATATGAGGTGGTTTCAGCCTCAAAACTTTGACAGAGCCTGATTTTATAGAATATTGCAGAAATAAAATCCGGGCTTCCTGTCCCTTGCTTTATCCCGATCCCGCTTCTTATCGGCCTTTCCTTAATGAAGAGCAAGAATCTGCAGACTTCTGGATGTCAGAGGGGCTGTCCCTGATCTGTACGTTTCCGGTGCTCTGGACACCTGGGCAGTGCTCTGTAGCCAGGACTTCGGCCTCAATGAACCACTGAGAGAAAGTCGCTCTACGCCGTTCCTCCCAGCACCTGCGTTACTCTGACTCACCCGAGGGAAACGTGCTGACGCTTCCACAACTCACACACAGCTCCCGTGGAACGAGAGGGAATCCACACAGGAGTGGTGTGTAAGCTCACTTTTGCCGATTTGGCAGAAGACCATGGCTTCTGCTTTTGTCCAGAGCTGGGGACAGTATAGAGACAGCCCCTCCAGTTTTCCCCACCCCTTCAAAACAGGCAGTAAACACTGCTAGAAAACTCGAACTCAAGACCACAGCGTAAGGTTTTAAGGAAAGAAAAAGGATTACATTTGTGGCGAAAACACTTGAAAGAAACAGAAATCTGTTAGGATTCTGTCAGGATGTCAGAATTGGTTGTTTTCCTATTTGAGACAACACTGAGATCTGCAGGGGTGGGTCTCAGGGTTTACACCAGGGGGGTCTTCCTTTTCCTGATCTAATGCTATAAACAGTCCTTAGACCAGACCACAGAGCAGAAAGAACACACCAAGTTCATGAGCCCTGGCACCACGCCCTTCTCTGCATCCTCCACTGTTTAGGCATCTGAATTTCAGACAGGGATAGGGAACACTGAGGGGAATGAAAGGAAAGAATTTAATAGCTTAACATCGCCTGCCTGCCTGCCTGCCTGCCTGCCTTACAGGCATCCAGCCATCTCCAGGACTATCTCCCTAGAGAGATTTTCAGGCACGTTCACCTGGCATACCATGATCTACCCTCCTCTCTCATCCGCGCAGACCAGGCTTTGGCCAGCCCAGGGCTCACCCCGACACTTACGTCCAGCCCAATACCGTCTTTTCCACTTTGGGGGTTTGGCACCTGTGTCACCTCAGCTCCAAGTTACTTGTGAACATTATCAAGACCATCTTGTTTTCTGGCCCACCCCTTCACATACATACCTACCCCCACCCTACCCCCACCCTACCCCCACCTCTGCCAGCACAGGGACAGCGATGTTCTCTGCATGCTTGCGTGCAAACAAGCAAGCCCAGAAACAAATAAATAGGTTTTAAAAAAAGGTTTTAAAGAAACAAGATGAAGCTAGTCTTAGTGGCATGTGACTGTAATGGCAGCACTGAGGAGGCAGAGAGAGGATAAGAGAGGGGAGGGGAGGGGAGGGGAGGGGGAGGGGGAGGGGAGAGAGAGAAAACGAACCATGGAATAAGGCTTACGATAGCCCAAATCAATAAAACTGCTTGCTTTCACAGAGACTTGTTGTTTGATTTGGTTGCTATTTATTTTCTTGTAGTGAGAAAATGGCATGAGTTCTAGTTCCTGACAGGCATTTCAGCAAGGACACAGTACAGATTGTAGGGATGGTGCTAGCCTGCTGATTCTAGAGCTTATCCACCCTGCACATCTGGAATTTCACCTGTGTAGATGAACAATTCCACCCCCGACTCAACCTCTGGCATCCATTGCTCTCCCTTCTGCTGCTCAAAGGTGGCTCTTTTTTTTTTAGAAACTTCGGATAAGTAGAACTCTCAGTACTCAAGAGGGTTGGGAATCACAAGAGTTCCCAACCAGTTTGGGATACATAGCAATAACCTAGTCAGGCATTGGTAGCACATACCTTTAATCCAAGCACTTGGGAGGAAGACATGGGATCTCTGTGAATTCGAAGCCAGCCAGGTCTAACATAGCGGATGAGTTCCAGAAAAGCCAGGGCTATGTAGAGAGCTCTTGGCTCAAAAGCAAAAATAATAAACAAAGAACCCGCCAGAATCCTGCAGTATTTGCCATCTATGACTGGCTTATTTCAGTTGAGGCTCATCTAGATTTAGTATGCAATGGGACTTCCTTCCCCTTTCTTCATAAAAGCCATCTCGACACACAACAGGTGCGAAGTCAAGGCTCACTGAGCCTTTCCTTGCTTTTTTCGGTGATATTGATCATCTTTTTGTATAATTATTGGTCATTGATGTGCATTCTTTGGGAAAATGTCTCTTCAAATCTCAAATCTGAGCTGGGTCTGCATGCTTTTAGTCCCAGGACTCAGGAGGCAAGGAACAGTTGATCTCTGAGTTCAAGGCCAGCCTAATCCACGGGGTAAGTTCCAGAATAGTCAGGACTATACAGAGAAACTCTGTCTCAAAAAAATAACAGCAACAAAATCCCGAGCCTATTGGATATTAGGTTTTTGTTTGCTCTGCCTTATAGAATTTCTCATATATTGTAGTGATCAATGCATTATCAGCAGAACAGTTGTGTATTTTATAGCATCCAATGGTTTACTATTTTCCAGGATTGATTGAGTCTCTTACTGTGCATAAACTTTTAAATTTCATTCGGCACCCATGGCTTATCTTATTTCTACTTTTGTTGCCTGTGTTTTTTGTTGTCACAGTTATGAAATCATTGTAAAGGTCAATATTGTAAATCTTTTCCCATTTTTCTGGTAGTTTTACAGTTTAATAGCTTCCATTTAAGTTATTGATCCATTTTTAGTTTATTTTGGTGAATGATGTAGCATAAGACTTCAAGTTCATTCTTGTGTGTGTGGACATCTGGTCTTCCCAATACCATGCATTACCAGGACCTTGTGTGGGAGCCCTAGAGTCAACCCATAAACCACATGAACACCAATTTCAGCTAAGCAGGAATGGCTTACTGAACGCACATCCCCAAGACCGATCGACCAGGGCAACAGCTCAAAGCTGGACATCTCTCAGGGACAGCTTATAAAGGCTAAAAACACAAAAACACAAGGAGCTCATACACAGATGTTGGAAGTGCTGCCCAGGGGTCAACCCTGACTCAAGTCATTTTAGACATAACAGTTCATATTGACCTTTAATTTGATGGGGCCTTCAGGAAGCTTATAGTTGTGGAATTTCTTAAGATTAACAAAGCTTATAACATATGGAACAAAACTGGGTATGTGGTCAGCATGACCCCTACTCCGAGTCAAGTTATTTTTCTGTGCCAATGACGGACAGACATATTACGGCAACAATATGAAATAGTTGGCAGGCAAGGAACCAAATGGCTACAGCTATGCTGGGCGGGGAGGCTTCAACATATGTGTCCTTTCCTCATTGTATACTCTTGATATCCTTGTCCAAGTGGA
U3_D5 96211854 96212603 750 TATTACCTAGCCTGGGAAATACAATCAGAAACTGTGGTTTAATTTTCATAACCAGGATTAAAGAGCCCATACCATTCCTGGGCTTAAGTCTGTGAGGTAATACAATCTTTACAAAGCAGAAGTGACCGCCTCCCCACCTGAGTCCTGTCTGTTGCTCGTGTTTACTTACGGAAGAGGTTGGGATCTGTCTTTATGGTTATCGTGAATCCATTTCCTGGTTCGTGGACCCGGAAGAAGACCATGGCCACATTCTGGGAAGACCTGATGCTCCTTCTGGTAAGACCACTCTCACAGAAATCTGTGTACCTCTTCATAGTGGGCAGAGGGTGATCCTGAGAACTTGGGAATTTCTCTCCCTTTAGGATCCAACCATCAAATACCTTCAAATGATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAACAACAACAACAACAACAGTACAGACATACACAGTCGCTTTCACCAGCTTCTAAAATCACAATGTGTTGTAAAGAGGGGACGTTAACAACCTTCGGTTTTTAAGTGGGGAAGCCACAGCCGGGACTCACAGCCTGCTTGCTTTTCAGTGGTATTTATTAAGACTGTGCAGTTTGGTTGCAAGCACTATCTTTTCTGCATAAGCCTTCAGGCTGCTAAGTTGGCACACTTTTTTATACCTTATCACCTAGTTTGGTAGAAGGGCATTAACTACAGACGACGCTGATTTTATTCATTCGTTTCCACATGAGATCAACT
D3_G3 96207586 96211740 4155 TAAGGAATGGAGACAACAGGGTCTGCTTATGTTTAAAGAACTTACTATGAGCATGACTGTATGCTTAGGTACTTCAAACGTAATTTCATTACATGATGAAATAAAGATTTAATTAGGCCCACTGTGCACAAGTTTGTGAACAGACATTCAAACAAGCTATTTAGGAGTGGAAGAACAATAATTGCCCTTCCCATGAGTAGATAGACGGAAGTTTCCACTTGGTGAGTGATGCCATCTCTCTCCTCTTTGGAATCCTCTCAGGTGTTCTTGTAAACAGTGGCAGAAAGTGAGCTGCATGAGGACCTGTCTGGGGTTCCGGGGCCTCAGCAGCTCCCGCGTGTCTTTGTTGAAGCGTTAAGCTCCTACCCCCTCTTGCTAGATTGTGACCCTGTGCCAAAGGCTCACTCCAGCTTGTTGAACTGGAAGCCAAATCCCTGCTGTTCGTTCTGAATGTCTGAAGTCTCACCCCCAGCACACATGGAAGCTGAGGTGCTTATGGGATGATCTTCAGTGGTTCACTAGCACAACCATAGAGTCTTCTCACGGGGAACGTGAGGCATCTCGCAGCATGTCCCCCAGCCCATTCGTCAGGTGGAAGCCAGAATGACCTTCAGGACAGCACACATCAGGTCCTTTCCCACCCAACCCCAAAGGCTTTCCCACTACTCCTGCTGCACACAACCTTCTCCGGGTGGGCAGCACACGGGGCTCTGCCCAACTCCCTCTCGAGCTTGCTTCTTCCTGTGTCTAGGATCCAGTAAGCCTGTTTCTGCCAGGGCAGCACTGTTCCATCCACTCCTCCTGCCTCCTATGGTTGCTGATTCTTCTAATCTAAGTTTCCTTTCCTGACCACACTGTCTCAGGCAGTTCCGTCTACCCACTTTCCAGCTCCTGTCTGTCTATCAATCCACTCTGCTGCATTTTCTGGACAGACTTACCATGATTTGAAATTCATGATAATTTTGCCTGTGCCATCCCACTTCAAATAGGCTCCCTGTGTGAGCAGGACCTGCCTTGTTCATTGCTTATTTCTAGAACCAAGAATAGTCCCTGAGCAAAGGAAATTATTATTGTTGTTGTTTGGCAGTACTAGTAAAGGGAAGGTCATGCAAGCCTGTCACAAGAAGTTCTCCTATCTCAGTCTCTCAGTACTGGGATTACAAGTATAAACCACCATACCCAGCTTTCAATACCCAGGTGTCAAAAAATAGGTTTTCAAAGGAATAGATGCCTAGAACACAGTAGGCACAAAAAATATTAAGGGAAATATAAAAGTATTCTTAGAGCTTTCATACCAAAATCACAGATTTCCTAATATGTTACCATGTATCCCACCAGATTCTAGATCTTTCATCAAGAATAAAATGGCTTACGAACACTGTCAGGCTGGATCCAAAGCACTCAGACATTCTTATCAAAGGATCGCTCCTCAATCAAAGTCCAAGGGCAACAAAGAAGAAGAAGCAGGGACACGTGGGTGTGGGGGCTGGAAGAAGTTAGATATCAATTCTTTCTCTCGGTCTTGTTCCTTATTTAGTTTTCACAAAGATCTGTATGTTTGGGAACACCGGCTGCCTCTGATACCACCAAGTGCACGCATTATCTTAACTTAGGCCGATGAACAAGGAAAAGGAAATGAAGGTGACTCATGGAGCAGCCATTGTCTTTGCAGAGGGATGCTGTGAAGCCAGCATTCATTGTAAGGCACACACGGAAGCTGATATGAAAGCAAGTTGGTTGAAATTTCACAGCATATATATATATACATATATATATATGTATATATATATGTATATGTATATATTAGACACACACATACATATATACACATATATAAATACATATACACATATATGTATATACGTATATGTGTATATGTATATCTGTGTGTGCACATATATATTAAGCAAGATGTCATATAACCTGCTCTTCAAAACTTTACTGCTTTTTGCAATAGAAAAAAATCCAATTTCATATTTTATGCCACCGTTAGCATTCTTTCTTAGCCTAGTACTGTCAATGAGAGTGACTTCCTTCTTATCAAAAGAAAATTCCAATAATCAGGAGAGCAGCTGTGTCGATGACTCAGAGGAGGCTCAGCTAGGAGAGGATTTTTGTAAAAGAGGAAAGTACAAGAGTGTTCGATTGTAATTACATGCTGCAGCTTTACCAACTGAAAGCATTTCCTATGTCACAAGAGAAATCCTTGAGTAGTCAGAATGGGCACCTGAGTGTTTGCCTGCAGCGTGACTGAAATAGACATTGAGGAAGCCAGGGCATCGTGCAGAAGACTTAAAAGTCAGGGGCCACATGCTTCCTGACAGGATGGGTTATTTGTCACTGATAAAACAAAACAAACCAAACAAACAAACAAACAAACAACAACAAAAACAACAGTGGCCTTACCAAATGGCCAAGGAGCAAGCAAGCCACTGACCTTTAACTGAAGGCCATGCAAGTGTCCGAGGGTAAGATCAGAGATTTTGATTGCCACTGGATAGATGATGGAGAAGCTGCAGTTTTGGTGCTGGTATGGAACCACTAAAGTAAACCTTCCACTCGGAGTCTGAGAGATGACATTGCAAGCTTAGGAGGTAAAAAAAAAAAAAGATACAAATACATTATTTAGAGTGCTGGACTTTTTAAAATAAATTATTTATGCCTTTGCTCTAGATTTCAGTTCATGGTCTAGTAGGTATATCCAACCTGGAGCCCAATGGCCACATGTGTCCCAGGATTGCTTTGAATGGTGCTAAACATATTTGTAGATTACCAACATTCAAGGGCTGGACCTCTCTATTTAGGTGTTGGGCCCAGGGAGAGGGTATATATCACCATGACAATCTGGGGAGAGGAAGGAGCAGGGGACTAGAACTCAGTGACATGTGAATATTGTCCGGAAGTGCCAGCCACAAGAGGGCTTCTCACCTTTGTCTGTTTATAACAATCCTAAGGTAGGAAGTCTTTATCAGTCAGACAACATACCTGGGGGTACCAGGAATACTTCTGTTGAGTTGATTCTAGATCCGAACTGGGAGAACAAGGCTCAACCACGAAGTCAGAACCTCACCCATAGAATTTGAAGCCACGGTGTTGTTTGTTAAACAATAAAAACCATGTCTAATTTTATTCAAAAGATTTCATGATACTGACAGAAGAAGTGGAGAATAAAATAGACAACAGTTTTATACCTAATACATATTTGCTGGGTTTAGAGTAATGTAAAGTTGACTAAGTGGTCTTTTTTTAAATTTTTATATGTATTATTGTTGTTGTTGCTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTAGAACTCACTCTGTAGACCAGGCTGGCTTCAAACTCAGAGATCTATCCGCCTTTGCCCCTAAGTGCTGGGGTTAAAGGTGTGGGACACCATGCCCAGCTTTAACCTTTAAATCGTGATAGTCTTAAGATTTTCTGAAATTTTTCTTTTTATTTCAAGTCTAATCTTACAACTGAGTTATTCTTCTGTCTGTGAAACAGAGGCATGGGATTGGCCTTTCTGCTGACCCACCGCCTTATGTTTTTAATTACTCTTAATTTCAGAAATAAGTATTTTTTAATGACTCTTAAATTCAGGAATAAGTGAAATTCCTTGACAGTTTAAAAAGACATCTTCTGATTTTTTTACCAAGCAAATGCCCACAGAAATGAACCCCCAACATTCTGTTTCTCAGATAGCCTCAGCTTTTCAGGGCAGAATTTCTTTCCCTCTCCTTCCTGTTTCCCTCTCTTCTCCCTAGCTTCTTCCCTCCCTAGTGCTAGAGTTTGAACCCAAGTTTTGCTTCAAGCATTCTTAGACAAACGGTTTACCACTTAACTACATCCCTAGTTCTCAGCTTTTCCCCTTAATGAGGAAATGAGCCGATGTACGAACTCGTTCTGCTCAGAATCACGCTCTTTCAGGCTGGCTATCTAGTTTTCCAGCCTAATCTATGTTTCTCTCTGTCAGAAATGACACAAGCTGGGGTGGTGGCTTTGTGGTAGAAGGTAACGTGGAAGGAGGTGATGGAGGGGCACTGGTTCACTCCCTCCTTAGGGGACAAGACAACATCTGCCCACCTGTCTCAGACTTTGTTTACAAAGCTCAGGCACCAAATGATCCCACAAGCTTATCCCGAAT
U5_15 96212675 96227725 15051 AACAGATCTCAAAGAATAAATAACAACAGCCAACCTGGATATGATGGAGGGTGATTGTAACCCCAGTACCACAGAGGCTAAGGGGGAGCACCGTGAGTTTGGGATACAAAGGGTGATTGTGTCAAAGTGATGGGGGGAGGGGCGTGGGGGGATCCAGAGCAGCAGGTGTCATTGCCAGTGCCTCACTCTTCACATTAATATATTATTATATATTAATTAATATATATATTAGTATATATCATCACCTCAAGCTACTTTTTAAAATATTTCTAAATGCTGAAATCTGGCAGTGCTGCCTGGTTAGCCATGTAAGACTTCCTCTTTGCCTCCTGGGACAAAGGGTCAAAGTCAGTTGACCCGGTTCTCAGAAATCACCGCAGGTCTCCATTTTAATGAGTCCATCATAGCACAATTACCAACTGCCGGGTTGCAAATTATTTCTAACAAGCTTGACAGGTTCACCAGTGGTTTTGGAGATCTAAAATGTGAATGTTTCTTGGAGCGGTAGAACTCTATCCTGTGGCCTCTGGGGGCTGAAATGGGATGTGTGGAGCTGGGAGTTCTCTTCCTCTTCTTGCTTCTCTCAAGCTCTACCTCCTGGCAGCATCCCTCTCCCAACCAGTGCCCCACTACTTCAAAACCACCATCAGCCCGGGCTCCTGGAATTACAAAATGAGTTCAGATCTCAGATCGCAAGCGAACCCTCCTGGAGAGCTCCTGCTAATTATTTTTGCCATGTTTTGTACGCCGCAGATTGCATCATAGCGTCTCACACTGCAAGTTCAAGTGCTAGGAAGTTCCCAGGAGTTGGGGAGAGCACAGAAGGGAAATTAAACCATCGACGTCCAGGGGCGCTAGACCTGCAGCGTTTGGCGCTAGACCCTTCCTAGGAATCCAGCCTCCGGGGCTCTCCCAGGGAGTTTCTGAGACTCGAGCACTTAGAGGTTTAGTTGTCCCCCACATGGACGCGCTTAGGGGAACGTGGGCTGGGGCCGCGAGTAGTGGGGACCCCCTCACCTTCAGGAAATCCCCGCCTTGGCAGTCGATGGAGACCAGGTCATAGTGGATGGTGATGAATTCCTCGGGTTCGCCGATGAAGAAGGCGGCGCAGTGCAGCTGCGGCCGGTCTGCGGTGAAGGTGAACTGGCCAGGGAGGCTCAGCATGTCCAGGCACCCTGGGAGAGAGCATGGAGCCCGGGGTTCCCAGCTGTCTTGGGAAGCTGCTCTCAGCGCGCCCCATTCCCCGGCTCCGCAGCGCGCCCCAACCTGGAGCTCCCAGCTTAGGCTGCACTGGGTTCCCTCGCTCTGGCTTGGAAGGAGCCGCGTGGATGCTGCAACCCAGGGGTCCCAGCCAGCCCCCCCCCCCCCGCCCCGACCAGATATCCTGCAAGCCTGTGTCCTAGGTTCTTAGCCTCCCGCCTCCCGGGCACCCACACACTGGACAGCCCCAACTCACGCAGAGCCCGTCGGTACGGCTGCTCCTCTGCCAGGTCCCGCTTCAGATTGGCACTGAAGAGCAGGAGAGGGTCGTAGACCGCGGCTTCTTGCACCTGGAGAGGAAAGTGGATCGTCTGGAAAAGTTCAGGGGGAGGGAAAGAAAGCGAAGCAGGAGCAGGGAGCCCTTTTTGCAGCTCAGAGAAGCAAATGGGGCAGGGGGCAGTGGGGCATGTCTCCTGAGTTCAGAGTTTATGCGTGAGTCGAAGACTATTTCCACCAGATTAGGGTACCCAGCCCAGAGAAAGGCAGGCAGTTAGGATGCTCAGGGGCACGGAGAAAGCTGTCTAGGTGAACCAGGGAGAAAGGATCAAAAGTTGGTCTGCTGTCCCTGGCTAAATACTCAAGGGATAGAAAGGTGTTAAGCTCGAGAAAGGATGGCTCACCTCTAGGTAGCGGCTCTCTCCCCTTAGAGCTGTCAGGAGGATCAGAATGAAGTGACATTGGAGTTTGAAGTTCGGTGACATGCTGGCCTTGTGTCTGCAGCTGGAGTGCTCTGCTGCGGAGATCTTAGGCGTTTCTTCCCACACCTTCGCCCACACTGAGAGCTCAAAGCAGCGAGGGTCCCTTTGCTGGGTCTCTTTTATAGAGCAGTAGAGGGGAGGCTCTCTGCCCCTTAACTGATCTGAGCTTAGTTACCATGGGTTACCCTGCCTCGGTGACAAAATGCGTGGCAATGACGAGGGTCCAGCTCTCAGCCACAAATATTTCAGCATGGCCCCGCATTATTCATCAAGAGTGACACTTCAGTCACGAGGACCTAGTGACCTGAACGCATAGCCGAAAGAATGAGGCAGAGGCAGAGAGATTCGGGGAGAGCCTTTCACTTGGCCTGTAAAACGTTACATTATATTCATTATACATATCTGGGGAACTTTTGGTTTTATAAAAGCAAATGACAGACATGTCAGACACATATGACCTCCTCATGGGGCCTAGCCATATAACTGTGGATGGGACTCTGATGGGAATGGCCAGAGCGTAGTATCAGTACGGCTATCATTCCCCTCCAGAAGAAATATGAGGTGGTTTCAGCCTCAAAACTTTGACAGAGCCTGATTTTATAGAATATTGCAGAAATAAAATCCGGGCTTCCTGTCCCTTGCTTTATCCCGATCCCGCTTCTTATCGGCCTTTCCTTAATGAAGAGCAAGAATCTGCAGACTTCTGGATGTCAGAGGGGCTGTCCCTGATCTGTACGTTTCCGGTGCTCTGGACACCTGGGCAGTGCTCTGTAGCCAGGACTTCGGCCTCAATGAACCACTGAGAGAAAGTCGCTCTACGCCGTTCCTCCCAGCACCTGCGTTACTCTGACTCACCCGAGGGAAACGTGCTGACGCTTCCACAACTCACACACAGCTCCCGTGGAACGAGAGGGAATCCACACAGGAGTGGTGTGTAAGCTCACTTTTGCCGATTTGGCAGAAGACCATGGCTTCTGCTTTTGTCCAGAGCTGGGGACAGTATAGAGACAGCCCCTCCAGTTTTCCCCACCCCTTCAAAACAGGCAGTAAACACTGCTAGAAAACTCGAACTCAAGACCACAGCGTAAGGTTTTAAGGAAAGAAAAAGGATTACATTTGTGGCGAAAACACTTGAAAGAAACAGAAATCTGTTAGGATTCTGTCAGGATGTCAGAATTGGTTGTTTTCCTATTTGAGACAACACTGAGATCTGCAGGGGTGGGTCTCAGGGTTTACACCAGGGGGGTCTTCCTTTTCCTGATCTAATGCTATAAACAGTCCTTAGACCAGACCACAGAGCAGAAAGAACACACCAAGTTCATGAGCCCTGGCACCACGCCCTTCTCTGCATCCTCCACTGTTTAGGCATCTGAATTTCAGACAGGGATAGGGAACACTGAGGGGAATGAAAGGAAAGAATTTAATAGCTTAACATCGCCTGCCTGCCTGCCTGCCTGCCTGCCTTACAGGCATCCAGCCATCTCCAGGACTATCTCCCTAGAGAGATTTTCAGGCACGTTCACCTGGCATACCATGATCTACCCTCCTCTCTCATCCGCGCAGACCAGGCTTTGGCCAGCCCAGGGCTCACCCCGACACTTACGTCCAGCCCAATACCGTCTTTTCCACTTTGGGGGTTTGGCACCTGTGTCACCTCAGCTCCAAGTTACTTGTGAACATTATCAAGACCATCTTGTTTTCTGGCCCACCCCTTCACATACATACCTACCCCCACCCTACCCCCACCCTACCCCCACCTCTGCCAGCACAGGGACAGCGATGTTCTCTGCATGCTTGCGTGCAAACAAGCAAGCCCAGAAACAAATAAATAGGTTTTAAAAAAAGGTTTTAAAGAAACAAGATGAAGCTAGTCTTAGTGGCATGTGACTGTAATGGCAGCACTGAGGAGGCAGAGAGAGGATAAGAGAGGGGAGGGGAGGGGAGGGGAGGGGGAGGGGGAGGGGAGAGAGAGAAAACGAACCATGGAATAAGGCTTACGATAGCCCAAATCAATAAAACTGCTTGCTTTCACAGAGACTTGTTGTTTGATTTGGTTGCTATTTATTTTCTTGTAGTGAGAAAATGGCATGAGTTCTAGTTCCTGACAGGCATTTCAGCAAGGACACAGTACAGATTGTAGGGATGGTGCTAGCCTGCTGATTCTAGAGCTTATCCACCCTGCACATCTGGAATTTCACCTGTGTAGATGAACAATTCCACCCCCGACTCAACCTCTGGCATCCATTGCTCTCCCTTCTGCTGCTCAAAGGTGGCTCTTTTTTTTTTAGAAACTTCGGATAAGTAGAACTCTCAGTACTCAAGAGGGTTGGGAATCACAAGAGTTCCCAACCAGTTTGGGATACATAGCAATAACCTAGTCAGGCATTGGTAGCACATACCTTTAATCCAAGCACTTGGGAGGAAGACATGGGATCTCTGTGAATTCGAAGCCAGCCAGGTCTAACATAGCGGATGAGTTCCAGAAAAGCCAGGGCTATGTAGAGAGCTCTTGGCTCAAAAGCAAAAATAATAAACAAAGAACCCGCCAGAATCCTGCAGTATTTGCCATCTATGACTGGCTTATTTCAGTTGAGGCTCATCTAGATTTAGTATGCAATGGGACTTCCTTCCCCTTTCTTCATAAAAGCCATCTCGACACACAACAGGTGCGAAGTCAAGGCTCACTGAGCCTTTCCTTGCTTTTTTCGGTGATATTGATCATCTTTTTGTATAATTATTGGTCATTGATGTGCATTCTTTGGGAAAATGTCTCTTCAAATCTCAAATCTGAGCTGGGTCTGCATGCTTTTAGTCCCAGGACTCAGGAGGCAAGGAACAGTTGATCTCTGAGTTCAAGGCCAGCCTAATCCACGGGGTAAGTTCCAGAATAGTCAGGACTATACAGAGAAACTCTGTCTCAAAAAAATAACAGCAACAAAATCCCGAGCCTATTGGATATTAGGTTTTTGTTTGCTCTGCCTTATAGAATTTCTCATATATTGTAGTGATCAATGCATTATCAGCAGAACAGTTGTGTATTTTATAGCATCCAATGGTTTACTATTTTCCAGGATTGATTGAGTCTCTTACTGTGCATAAACTTTTAAATTTCATTCGGCACCCATGGCTTATCTTATTTCTACTTTTGTTGCCTGTGTTTTTTGTTGTCACAGTTATGAAATCATTGTAAAGGTCAATATTGTAAATCTTTTCCCATTTTTCTGGTAGTTTTACAGTTTAATAGCTTCCATTTAAGTTATTGATCCATTTTTAGTTTATTTTGGTGAATGATGTAGCATAAGACTTCAAGTTCATTCTTGTGTGTGTGGACATCTGGTCTTCCCAATACCATGCATTACCAGGACCTTGTGTGGGAGCCCTAGAGTCAACCCATAAACCACATGAACACCAATTTCAGCTAAGCAGGAATGGCTTACTGAACGCACATCCCCAAGACCGATCGACCAGGGCAACAGCTCAAAGCTGGACATCTCTCAGGGACAGCTTATAAAGGCTAAAAACACAAAAACACAAGGAGCTCATACACAGATGTTGGAAGTGCTGCCCAGGGGTCAACCCTGACTCAAGTCATTTTAGACATAACAGTTCATATTGACCTTTAATTTGATGGGGCCTTCAGGAAGCTTATAGTTGTGGAATTTCTTAAGATTAACAAAGCTTATAACATATGGAACAAAACTGGGTATGTGGTCAGCATGACCCCTACTCCGAGTCAAGTTATTTTTCTGTGCCAATGACGGACAGACATATTACGGCAACAATATGAAATAGTTGGCAGGCAAGGAACCAAATGGCTACAGCTATGCTGGGCGGGGAGGCTTCAACATATGTGTCCTTTCCTCATTGTATACTCTTGATATCCTTGTCCAAGTGGAGTTTGCTGTTCTTCTTAATGGGCCGGTATCTCTCCTTATGCCAGGACCACAGCTGACAACTCTGGGGTCTAAATTTTGAAGTCAATAAGTTTGATGCCTTCTGGTTTTGTTTTTTTGTTTGTTTGTTTGTTGGTTGGTTGGTTGGTTTTGTTTCCTTCAAGGTTTTTGTTTTGTTTTGTTTGAGGTTCCATATTAATTTTAGAGTTGTTTTTGTATCTTTTAAATCCAACATAGATTTTATTATATTAATGGAACGATTTATACTCATTAAGATTATTTTTCAGCGGTATTAAAAATCAGAGGGAGCTAAGTGAATCTGATTTGCGGACCTAGTGTGAGTCAGACTTTGGATGATACTCTCATGTTTCAGCTATAGAAGATCTCCAGTTGAGAGAAACTACACGTTTGGGAGGTAGGGCTCGAGCGATAACAAGCTGAAGTATAATCCGTACATTAGTATAATCTATTTTCAAGATATGAAGATTATTGGGTGTTTTTTTGTTTGTTTGATTGTTTGTTTGTTTTTTTAAATCCCAAATATTCAATTTACCAATGAAGACTCAAAAGCCAGATGCTAGGGTGAAAGCCTGCTAGCTCAGAGAGTCAGAGAAAGCACTAAGCTGACCTTCCTCCTCCACCAATGTCCCAGAGGGAAAAATGCCTTCTTTCTCCTTCTCCACACTGTCTTAAATGGCCTTCAACTCAAAGACCCTCCTTTCTTCCTCCTGTCCCCTTCCTGTTAAATGGTAGCTTGCTCAGCCTCTTGACCTATCTTTGACTATATTTAGCTCCTGCTTACGGAAAGCTCTTTGGGTTAAAGGTGTGTGCTAGGGATGAGCCCACATTAAAACAAAACCCCAGCTCACAACCTTGTGTTTCACAATGTGGTCAAATCCGGCAACAGAAGATGCCTTCAGAAAAGAAAAAGTAAAAGCAAGCACTGAGTGGCCACGCCCTGGTCCCTCTGTCTGTAGGTTGTGGCCACACCCTGGTCCCTCTGTCTGTAGGTTGCCCCCAGTTCTGGGAGCTTCACATAGGCCACCAAAAACTTTGTGTCACCATCAGACAGATGAAAACAAGCTCAAACTTACATTCTTAAAATCTTCACCCACAAACCTGTAGGCAGAGGTCAGAGGCCAGGGCAGAAGACTGTCATAACAACATTCCCTTGAGAAAAGGGGGAAAGCAGTTTCCATGAAAGGTGTTTGTAACTGGCAGCAGTTACTGCTTCCAAAACACAGCCATCTCTAAAACAGTTTAGCCTTTTCATAGAGTGAGCTATAATATCCATGCATTGTGTTATACACTAGAGCATGGTGCTATTTCTAGATAACAGACTGACTGTACGGTAACATTTCAGTAAACCACACAAACGCAAAACAAAACCCACATGGGTTCAAAGAGCTCTTTGATCATAAGCTTCCTTATCTATAAACGGTAGAGTATATGCTAAAAATTACTGTAGGTGTCAAACAGTGTAAGTAATGCAGACAGAATAGATGTAAATGACAGCTGTGGTTAAATTACCAATTCTATTTCATGTCTTCATCACCAAAACTAATGATAATATTTTTGTAAGAGGCATACATGGGATATGTTTTCATATCATTATTAAATTAGAATGAATGACTTCATATTAAAACATTAACTGACTTTAAAAGCCATGGCTAGAAAATTAGAGGACTAGATTAGAATCTAATTGCAGAACTGCATTTTAAAAGATGAGCCAGGAATGGGTCCCATTAAAGATTATGTGCATGACAGAGGAGCTGATTTCATCACAAGAAATGGTGAGAGTCAGAGTGGAATAAAACTGAGAAGAGAGAGGAATGGGAGCGGCTGATGTTACAAAGCAGAAAGAGGGGGAAATCAGGGCCTGCCAAGGAGGAAGCAGTTAGACCCTTATCAAGCATCGCTGAGTGAGATCATGTGTCAAAACTGAGACGGTTAGGACCAAGAAAGTGTGGCTAGCAAGAATGCATCTGAAGGGCACTGATGGGAGAGAGAAATGAAAGAGGTAAGGTTTGCCCAGATTCTCACCGTGAGCAAGAAGCCAAGCCCACACCACAAAGTTGCCTCTGTATCCAGCCAGCTGATGAATCCTTCTCTGAAGACATGGCGGGGGTGGGGGGGACACTCCTGGAAATGAGTCAGGACAATAATAAGTTGGGGCTTTAAAAATAACTAGACAAGCAGCAAAAAGTGAGACGGCACAGTCCCAGGCTGGAAGCAGCGGATCCGTCGCTGTCAGGGAGGCCGCACATTGCTCATCTTTGCGCAGGTTCCCTTGACTTCGGAGTGACATTATTTCCTCAGCTCACTTCAGGCTCCCTCTGAAATATTCCATGACTAACATCCAGTCCAGAGATTACAATGAAATGCCATCTCATATGCCTCACTCCTTGCATCAAAGGGAGGGGTACAGCAAGCTCCAGACCCAGCAGTGTGTGTCACTGAGCAGTGGTGACGTGCACTGGGATATGACATCACCTGCAGAATCTGCCTCTTCCCTGACAGAGGAGAAATAATATGCAAAGAGTTGTGCTTCTGCTGTCTTAAAATAGATCTAAAGGACAGGAGTTTACAGGAGCTTTTGAAAAGGACAAGAACAGAGAAAGATCTAGGTAACAATTAGCTATCTGTGGTATCTCCTTGTGCAGTGTCCCACCAATGCATTATACATATACATACACATATACATACATATGTGTGTGTGACAAATAAGACACCATCAATATCGATTGCCTCAGGCAATTGCATGGCTCAACGCAATACTGTGTAGTTAGTATATAGACAGCAGTCTGTGGGAAAAATCAAAAATTTCAGGCTGAGGGTAACCCTAGTTCCAGACATCGAGAAAGCCCTGCACCGGAACCTACATACTGAATATTTCCTACACTCAACCTCAGGAGCCAGAGAAACAATGACTATTGTCCTTTTGAGCCACTGAGGTTTGAAGAGCTAATACAGGCCCTCTCACTTCTAATCCACATTCCCTCACTTTCCCATAGGGGATCTCCAACTTTTCCAGCTATATGACAGCTTTTGTCTGTAAAGCCCTTGACTCAGTCACCATCTGTATCCATGCATGGCCTTCTTCCAAGACAGCTGCTTCCAGGCTGAGTCAACATCTGTAAAAACAAGATGCCTGGTGCCTAGCTGGGCTTGTGGGGGAAGAAAATGTGACTAAATGACAGATGCCGGTAGCCTCTAGACCTCAGCCCTGTGGCCAGCAAGCCCTCACCAGTTGGTGCTAGAACTCCAAAGCATCATGAAGCACCCTCCCCTTTTCTGTGCTGACATTTATGTTTCTGATTGTCACCACTAGGCCCATTTCCTCTCGGACACCTGGGGAAGGAAGGCAATATCATGTTGTGAAATCAGGGTTAGACAATATAATGGCTATCCTGATATATTCATTCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATAAAGCCATACAATATATAGACAAACAATATATAGAGAGAGATTATATAGTAATATTGTAGAGATAGATAGATAGATAGATAGATAGATAGATGATAGATAGATAGATGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAAATAATCTCTTTGTATCTCATGGTTTCAAAACAGATCAGAATGTTGATCAACTTTACGGTTAAAAAGTAAACACCAAGATCCAATGGAAAGGCTCTTTAGTGTCCAAAATTGTAACAATTTGAACAACAAAATAAGGTAATGTTAGACGCTAACCTGGTGTGTAGAATAAACTTCCACAAGCCTTGCTAGTGTTGATGAATGATTGAATAAATTGTAAGTGGAGAAACAAGACACAGAATAATCCCAAATCTCTTACACAGAATACACAGAAAACATTCATGATCACATTCTTCAATTGTGGGCATTAAATTAGTGATTTCTTTACCAAAAGTATGGGAAGGGTGAGCTAGGGGTAACATTAACAATAAGAAACCCAACAGAACACTGTGTCACCTGGATCATGGCTGGTATCAGTAATCAAAAATCATACTGAGGTACAACCCTTGATATAGCATGATGAAAATGGCAGAGATTTCACCTCTGAAACCTTCCTCTTTCAAACACTGTCAACCCCACAAAGAATCTGAGAAACTGTCACATCCAGAAGTAGCCCAAGAAGACATAAACATAAAAGCAGACCCTAGATGGGACCCTGGAACAAAAAAAAAAAGCATCAGGAAAAGCTAAAGAAATCACAGTAAAAGCTAAAGTTATTGGGGTGGGGCTTGTTTGTTTGTTTTGCTATACAGAAGATTTTTAGTTTGATGGGGCCCCATTTGTCAATTGTTGGTCCTATATCCTGCACTGTAGGAGATCCGCAAAGGCAAAGGCTTTGTGTTCTTTCTTCTATATTGACCTTTGCTTCAAACCTTAGACTGTGTGTATTTAACTTGGCGTTAAAGAAGCCAGAAAACTGGAAAGGGGCCATTGGGGAGGGTAGAAGACAGATTTGATGGATGGGCTTAGTAGAACGCAGGTGTTATAAACATGAGAGTAATGGGGAGTAGAGCTTTAAGTGGGGAGGGGAGGATAAATGAGAGGTTATGGAGGGATAACTTATACTAAGAGTATTGGGGGAGAAGCTTTATATAAACCTATTATTTTATAAGCTTCCAAAAACTATCCATATTTTCTTATAGTGTGAGTGGAGTCACCCTACATGGAGCATGAAGATCTTCCCAAAAGCCATTGTTTGTCAAACCAAAAAAAGCCCAGTGTTGAATGTAGGATTCCTCCCTTTGAGTTGCTCGTGTGAGAAAGAGACCCACCCCTAAATCAATACAGGCTGATCGACGCCATTGCTCTTGTTTGCCACCAGAATTTGATGGTAAGATGTTACAGGATATTTGATCATATTATGAGATGGTAGGCTGGAAAACCCTTTTCTTCTTATGGTGTGCCTCGGCCGTAGCTCATACCTTTAGCCCAAGAGCTTTCTGTTTATTGTAAACAGCTTGCTGTGGTGTGGCTCAGCCCTAGCACACACCTTTAAGAGCTAAAGTCGAGGACTTACCCTTGAGGTGGAGCAAGCTATCAGTTGACAGGGAGTGAACAATGGAAGAAAACCCAGGAAAGAGAAGGCAGGTCTTTGAGTTGAAGGACTTTTAAGACAGTGTGGAGGAGGAGGGGCTTTTCCTTCTGGGATGTGAGTGGAGTAGGAAGGGCAGCTGGCTGCTTTCTCTGCCTCTCTGACTAACAGGCTTTCACCCCAGCATCTGGCTCCCGAATCTTCATGGGTAAAATCAAACTTTTTTTTTAAACAACAGTAAGACCCCACTGCTGAAGATACCACACACTTTAATCTCGAGACATGAAGAAATCAAGGTAGCTTTTACCTGGAAGTTTCCTCCCTGCCAGCTAACGTTCGCATTGAGAGAAGATGCTATACAGGCCACGGGAAAGGTATCAAGAGTCTTACACAGCTGTGAACCCCACTAGCCACGGGAAAGGTATCAAGAGTCTTACACAGCTGTGAACCCCACTAGCCACGGGAAAGGTATCAAGAGTCTTACACAGCTGTGAACCCCACTAGCCACGGGAAAGGTATCAAGAGTCTTACACAGCTGTGAACCCCACTAGCCACGGGAAAGGTATCAAGAGTCTTACACAGCTGTGAACCCCACTAGCCACGGGAACGACAAGCCTGAATATATTCCCACGAGTGCAATGTGGTGTAAGTGTTATGGGAGTAACTAACCTCTGGTCAGATTTAAAACCAACCCCACCAGAGGAAACCCATTCCTGGCTCTGTAACCTGGCCAACGACCAGGGGAGTCCCTGGTCCCTAAGGGAGAACTTACTGCTATTATTTTGCTAACTGGACATGATCCAAAGCTACCTTCCAAATGCTTATCTGTGTGCCAATAGATTACCTCAGCTCCCATCAAAGAAGCTTCCTTACACAAAGTGGATGATGGTTAATACAGAGCTGGTGGGAACTGGGAGTGGGAACAGAGCTGATAAAGGAGGCGGACCAAAGTCTCATACAATAGCTAACTTTATTCAGAGCATCAGGTAATTTAAACTCTGAGGGTCTGAATAAAGTTCCCATGAGTTCAAGTGGTATACAACCATAGGGACAAGCCACACTCAGCAAAAATGTCCTTCCATAGAGGCACATAAAGGATACTATTTAATTGAATAACATCAGCAAGCAATAGTGAGCAATCTAAAGTAAACTCACTCCTATCCACCACTACACCTGGGAGAAGCATAAAGACAGACTCCGAAAACAAGCCTGTGTCTCGAAAAAAAAAAATTTCTCACAAGCACTCAGAATTCCCCTCACATGACCCCATTCTTGGGAAGTGTTCCGACAACTCAAACCTGCCATAGTGCAGAGAATAATTGTCTGTGGAGTGTTCAGCCATAACTAGGACCACCGTATAACATGTCCTTCCCTCAAGACTCAGGGAACATTGCAGAAGGTTTGATCTTACAATGGGACAGAATTACAATTCAGAGCACGGGAAGGACTTCAGCAAAACAGTGTCTTCTGAACTTGACAGGGCCATGGACAAATGAGCTCACAGAACTCACCTGTATAAGACCTGTACACCATAAAACCAGTCAGGGCCATGGACAAATGAGCTCACAGAACTCACCTGTATAAGACCTGTACAAGATAAAACCAGGCAGCACTCCACCATGAAGTGGGGAGGACCCATGAGGCCCCACATCCCCAGCTGTTGGAAGTTGATGGCTGCCAGAGTGTCTGTTTTCTTCAGTGATGTGGCCCCCAATAGGTTGACCACACTTCAGCACATGGCTCATACCTGTGCAGAGACTACTATACTAACTGGACTCACTGGATTTTTTTAAAGACATGGAAGTAACAGGATCATGTGATCCAGAGGCATTGGTAGAAGAGAAGAATGTGATCAAAATCCACTGTGTACATGTATGAGATTATCAAAGAATTAATTTTAAAATACTATTTTGTAAATTGTCTCTTGGACCACCAAGATGGCTTAGTGGGTAAAGGTGCTTGCCACCAAGCCTGAAGACCTGAGTTCAATCCCCACAACCCTTATATACAGTGGGAGGAGAGAAACCACAAGTTGTTCTTGGTGCTGCCTCCTTGCTGAAGGAGAGAAATCTGTTAATAGAAATGTGAATGGTGTTTGCAAAGAGTAAAAGCGGTATTGAGCTCAATACATCACCATGATTTTAGAGGCATAGGTTTTTTATATTATAAAAACCACCATAATAACAACAAAAAACATGCTCATGGCTGGATCAAAAAAAAAACCAACTTAAAATATAGAAAAACCCCTCATACTGCCCCTTTAATCCTATCATGAATATACACCATGGTTAATGCAGCATTTATCTTTTCCAGAGACTACACATGCGAGATTTTCTTTACAGAAATGAGGCCATAGGTCACATGTTTACAAAGCAATTCTTTTCATTTCAAGTCACAAAAACATCTGTGTATGTATGTGTGCACTCCCTTACATTATCCAAATGGCTGTACAGCATTTCATCAGCACAGAACACTATCTTATTTAACCAACCTTCTATGGAAGAGCACTTCTACTGTTTCAAATAATTTACTTTTGTTGTCCAAATTATATATGTAAATCCCTTATCTTAGTTCTCTAGACTTGAAAGTAAACACTTAAGCCAATGAATTCTCAACTGTGGTATCTCCTTGTGATGGGGATCAAAGGAAAAACAAGAGGCCAGAAAGACAACATTCATTCACATACATAATACAGAAAGGGAGGCAGGTCAATTATTTTTCATAAAGTCATGGACACTCTGAGTCAGATGCACACCCCCAACACACACCCAAGGCCTTCCTCTCAATTTAGAGTTTGAAAGGGAAGGAGATGAACCCTGAGGTCAGACCGGGCAAGGTGGCTGCTTCATTTATGATGCTGAGATGGATTTAGATCGCCACATATGGCATCTCATAGCACACGGCTCCCGCCTGTGCAGAGCTCTGGGTGTGATGAGATGAAGAGAGGTCAAATTGACCTCAATGATGTGTCAAATTAGTAATCATAGCAATTCCACATTGTCACCTGCTCTTTGGCAGGTTGGATACGCCACGTGTAGGATTGATTATGCAAATGACTCCTGTTTAATTAAAACATCTGTTCCACATGTTACAAGCCCTGACGGTGAAGGTCTTACTGAAGGTGAGCTTCAGTTCCACATGAAATGTGACGTGGCTGCCTTGCATGGTGGTTTGCCGGCATCTCGCTCACCCTTTCTGTCCTTCATCTCTCATGGCCCCCATTGGGTGCGCTGGACAATAACTACTTTGTTGTTCATTCATAATACATATTGACATGAACTATAAAAATGAGAATAGAAACTATGTAATAACCATGAGAGATTTATCTTTTCCCTTCATAAAAGTCTGTCTCAGAAGGCCACAAAGGACAGGTAAAGAGGGCACACCAGAAATCATTCTAGGCGGCCTAAGTTGGAAGCTTAAGCTTTGGCAATGCCTAGAGTTATGTCTTTATCCATTCCAAGCTGCTATTACAAGACACAACACATTGGATGATTTATAAACAAGAGAAGTTTGCTTCTCAGAGTCCTGGAAACTGAGAAGTTGCAGATCAAGGTGCCAGTGGATTTGTGCCTGCGTAGGGCTACCCTCTGCTCCCAATATAATGCCTTGATGCTGTTTCTTGTTGAGAGACAAATGCTACGGTGTCATATGACAAGGAATGGCCCTTGGGAAGGCATTGGTCCTCTTCCTGGAGATAAAGCTCTTATGACTCAATCACTTCTGAGACCCTGTCTCCTCATATTCTTGCATTGGGATGAAGTTTCTAAACAAATCTTGAAGACATCATCATTCAAACCATAGTACATATTATATTTTACTTTAGTGAGATCTTTGGAAAAACTTTTAGTTGGTTACAATGGCTTCATTTTCTCCTGTTCTGGTTAGGATTACTGTCGCTGGGACAAAACCATGTAAGTTGGTGTGTGGGGGTGGTGGGGGTGGTGGGGTTGGTATTCAGCTTTCACCTCCACACTGTAGTCCATCACTGAACGAATTCTGATCAGAAGCTCAAGAAGGGCAGGAACCTAGAGGCAAGAGCTGACTCAGAGGCCACGGAGGGGTGTTGCTTACTGGCTTGCTCATCATGGCTTGCTCAGCCTGCTT
D3_15 96196692 96211740 15049 TAGTTTAAGCTAGTCCAGAAAATAGCTTCATTGTAAAATCTTGAGAAGTACTTAACCAAATGTCTGAGCACTGTGGCTTCAGCAAATGGAGACAGGAAAGGTGATAGTGCCTTTGGACCCTAGAGACTGGAGCTCCATGCTACCTTCATGTGCACGCCCCATGGCCACTCCCACTTTCACAAAGGCCATGTTTACTATGGCTCTCATTCAAAGCTATGGTCAACGGTTTACATGGCCAAGTTAACTTGTGATATGAATTAGTGCATTAACTAATTGACTCTGTATTCTTGAACCTTTAAGAAAATCGGGGAGCTGCTACGTTTTGTCCTAGTTTGGGTTTTGTTGATGTGAAGAGACACCACAGCAACTCTTATAAAGAAAAGCATTTCACTGGGGATGGCTTACAGTTTTAGAGGTTTAGTCCAGTATCATCATGGCTTGGGAGCATGACCATGATGCAGGCAGACATGGGCTGGAGAGAGAGCTGAGAATTCTACAGCTTGATATGAAAGCAACCAGGAGTGTCCCTCTCCTCTGCCCCGAGTGGCTCTCAAGCATAGGATCTTAAAGTCTACATCGACAGTGACACACTTCCGCCAACAAGGCCACACCTACTCCAATAATGCCATATCCACTCCAACAAGGCCATACCTACTCCAACAAGGCCACACCTCCTAATGGTGTCACTCTCTGTGGGCCAAGTATTCAAACACTTGAGTTCTATGGGGGCCAAACCCATTAAAACTCCTATATGGTTTAAATATTAAATGCCTCCCAGAAGTTCTCAGGTAGAATGCTTGGTCCCTGGATGATGTTGCTACCTTGGGAGATATGAGTACTTTAAGAGACATGTCCTCTCTGGAGAAAGTGAGTCACTGGGGATATGCTTTCAAAGGGGATACTTAGTCCCTAGTTGCTTCCTCTTTCTCTCCCTGCTTCCTGTCTACCATGTGGTGAACAGTATCTCACTCTATATGATGCCACCACTACAATGTTAGTTAGGCCTCACTATGACTCACTATTCTGGAACCCAGAATTCAGAGCTGGAACCCTGAAGCCATGAGTCAGAGCACATCCTTCCTCCCTCTGTTGTTTGGGTCAGCATTGAGTGGGATGAGGCACCCAAGGCAACGCAGGAACGAGCGTGTTACTGCTCCCTGCAGTGCGTTTTTAAAGATGGAGAGGAAGATTGTAACTGTGATGTCAGTGAAGGGCAACTGTCCAGTCAAGTGGGCCTTTGCTGTCCTTCAAATCCTCCCACTTTCCCTGTGGACCTGAGGACTTGATTCTATCTGAATCAACACAGCCTTCATTCTCTAATACTCCGAAACCTGGAGAAGGAGAGAGGTCAGCAGGCAACACCCACCTTTTGACTAGGGAGCTCTAGCATCTGCCCCGCCCTTCTAAGGATGAGGGTCATTGTTAGATACTCACAGCACTCGTGAACCCCAAGAACCCCTATATAAGCACTGCACTTTGTCCAATTCGCTGCTGCTGTCTGCTCCCTCCTGGTAGCCCAGGATTCATCCCTCCACACCCTGGACCCTCCAATAAATCTCTTTCATGAGTGAGATTTGCTGCCTGGTGTGACTCTGTCATCAGAAGACAAGAAGACATGCAGAGGAGGAATGAGCTGAGGTTCCCGGGCTCCTCAGCTCAGCTGCGACCCTCCCCTGGGAGTTGCAACGCCTCTGCTGAGGAGGCTTCCACAGAGCTGTGTGGCTCCTTGTCCTCCCCTGTCTCAGAAGTACCCTTCCTTTGGGACACTGTCCCACCTCAGAGCTGTGTGGTTCCTGGGCTTCCAAGTCCCAGGACACCTACCTTCCCATCCAAGCAGAAAGGCCAACAGTCATGGCTCCATCCTGTACGTCCGCCCTTCTATCTTAGTGCTCACACTGAGTCTTTGCCCTGCATACAGCACAAGCCACTTCATCCTTCCTTAGCTGCTGAGGGGCCGAACACACACACACAGGAGCAGAGTCCACGGTCCAGCCACAGACTTTGATTTTATTATGTGTCCCAGTCCTCTCATCAAGCCTTTCTTTCTCTCACTTAGACTGACTCTCTCTCTCTCTCTGTCTCTCTCTCTCTCTCTCTCTCCTTCCCTCCCATCCTCCCTCCCTCCCTCCTACCTCTGAGTTTTCACTCTCTGCCATTTGTCTTCACACACAAAGAAAAGCATTTCAGATGTGAAGAGGAAAGTGGACAGAATCATTACAGATGACCCATTAGAAAGAAGTCACCGGCTTGTTTATTCACTCTTACAAAGGTGTCTTTTCTGCTGCAGAGAGGAGCGTATTCAGATATAATTCCCCATGATTACCCTTTGATTGGAGATTCTTTCCCAGCTTCTCCTGGGTATTTTGAGCAAACAAACTCCCTTTAAGTACATCCCATCCATCAAAAACCCTTCTTCCTCTTTCATGAAGACTACTTTGCAGGCAAACATGCTCTCCCTGTTAAAATGGCAAGAAAAGAGATTTCACAGTTGAGACAGACCTGGAAAAGAAATCTTTGTCTGTAAAATTTGTTCAGAGAGCCAAATGTATCTCCTTCTCTGGGAAGAGCTAGACAACAGAGGAAGAAAAACCTCATGAAAGGCAGGTAAACTTCAATGGCTAATTGGGAGCAGAGGCAGCGAACTATCTGCCCCCACTGAAGGCAGCTTAATGAGAGCTTTGAGTACCGCTGAAGAGACAGTGAGTGGACTGACATGGCGCTGATGATTTAGGGGGTCCTCATTCTGGAGCTCTAAATGGGGTACAGGGATCTGTGAACTCTTGAGCAGAGTTTGTAACTATATGGGATTTCTTTCCCGGCCCATGAGCCATCGGTTGTCAAGTAGTAAGAATTACCATCTCAAGACAATCCTCTCGGGGAAATAGACAAGTTGGACAGGACTATATCAGTGTGAAACTTTGTGTTTATGATATCAAAGGTGCACATTTAAAGTATTTTTCTTTGGAAGAGAATCCAGGCAGGCCTGTCTAGATAGTTAACAGCCAAGTTGGAGAGGAAAATAGAAGAATGGCTGAGAATTCAATTTAGACAATTCCCCAGTGCAAAGAACAAAGCGACTTCGTGGCTTGCCAGAACAAAAGCTCTTTACCCAGAGTCCAGTGTGGCCACTCCTAGTCAGTGAGCATCCGTCTCAGAGGTCATACAGAGACTTCAGGCTTCTTGCATCCTATGTCTCTGCCTTCTGATGACTTTAGAGAGTCCTCTGTCAGAATCCCCACATTTAAACAATCGATAGGGGATCATATGAGAAAAGATGCCACTTCCCTCCACATCCCTTCAGTCAGATGACCAATGCTCTAGCAAGGGAGGTTGGGAAATGTAGTCTTTACACTCAGGAGGTTGAAGAGCACATTACACTTGTACCTGCTTCCATGAAGCAACAATACCAGAGTGTCTAGGATTGCAGAAAACACGCTGACTTAGAAACTGAGAGAAAATCCAAAATGTTCTCATGGACAAAGTAGTCCTCAGATGACACGCAGGAGAGGCAGGCTTATAAGAAACAGGAAGATGGACAAGTGTCATAGTACAAGCCTTTAACCTCAGTTCTTGGGAGGCAGAGACAGGTGAATGTCTGAGTATGGGCCAGCCTGATTTACATTCCAGGATATATGGGGCTACCTAGAAGCTAGAAAGGCATGCAGGCAGTGAGAGGATCACAGTCATATGGAACTCTCTCTCCCTCCCCCTCCCTCTCTCTCCCCCTCTCCCTCTCTCTGTTAGAGGGCAACTCTTGGGAGTTGGTTCCACCATGTGAGTCCCAGAGATCAAACTCATCAGTCAGACTTGGTGGCAAATTACTTTGTGTGAGGAGCGGGTGTGGCAGCAGTCCCAAGATGGCGCCTGGGACTGCTGCTAAGTCTTATGACTTGCACCTGACTTCCTCATACACCTGAAAATAAGCCACGACCATCGTGAGAGCTGCACAGGTGCACCATGATGCTGGCGGTGTAAAACAAGTCCATATTTGGTGGAGACATGCCCCTGCCGCCCTGATTGGCTGAAGCCGCGTGCCTGGTGAGGTGACGTGGCCTGCCGTGAGTGGATGGGGGCTGAGAGTATATAAGAGTGAGAGACCTGGGGTTCGAGTGAGAGAAATGAAGAGAAGAGAGATGAAGAGGGAGAGAGATGAAGACTGAAGCTTGCTGAATAAACTGCTGTTAGAAGAACTGGTGGTCGTGTCCTTCTTGCTGGTCGAGAGCAGATGAGACAACTTTGCACACCGAGCCATCTCACACCCCCCACCCCCACCACTTGGCACGGGCAGGCTCTAAAGCAAAATACTCTGCTTTCTACAAAGTTGCCTACAGGAGCTGCTGGATGGGCCCTCTGTGGTGAAGACATACACCCTGAGTTTCCTCGGAGGCTTGTGGAAACACGCCTGCTCCCCGTTTCCTGGTCAGGGACAGTCAGTACTCACCCAGCAGTCACCCTCCGCTCTGCATAACCCAGTCAACTATTCCAGACACTCCAGAGTTCTAGCAACTCCAATAGATCCAGAATAGTTGAGTCCATAGCTTGGGTCTGCCCCCATGGAAACCCTGTTTTGGAGGTTTGGAGGGAGGTAATGAAGGCAGGGGCCGCCCAGGAAAGCCAATACATCAGACAGTCGAGACGCTAACATTCTGCATACGTTTATTTACATTTGGACTTATGAATGGAAAGGGGGAGGGTTGTTCCCATGCTACAAAGTGTTGTTTAAAAAACAAGTACTAGCAATTAATAATGGATAGCTACAATTTGTGAATGGAAGTATGAAGTAAAAATAACAGATGCAATTCCTTTGGTATACTCAAGACTTTGACTTCTTCTTTTTTTATTTATTTATTTTTATTCTGTCATGAAACTGTGAGTGCCACCATTTCTCTGTCTTTGGAGCATCTGCCCTTGCACATAGCTCTTTTCCCACTTTCCCCAAGGGTCCAGTGACCCTTGTTCCACCACTTTGTGTTTGCAGCATACCAAAAGCACCAAGCATGTCCACCAACAACCTGTTGTCTACCTTCTGTTTGTTGAAACATTATAACAAAAGGAAGCAAAATTGGAACTCATGTGTGCACGTGTTCAAGGCCTGGAGTACTGATTGGAAGACTTTCCATTTGCTGCCCATGAAGATCACATACAGTAACTGTGGAGAACTCACTGGGCTAACAGCATGTCAATCACTGAAGCCGGCCATTCACAGACTAGACAAACAGTATTCTGGGATGCTGTTTCCGGTCGAGGTTTCTAGCTCGAATGGCTCCAGCTGACGATACTCAAATGTCACACGGTTAATGTGTTTCCCACTGGAGACCATGCGTACCACAGCATTGTCGCAGCTAATTTTCATCTGGGCTGGAATTGGGGTGGGGGAGGGGAATAAACATTTTCCAGCAGGTTAGAGAACAATCGGTCAAACCAGTGCTCTCACTACCTAAGTTAAACAGAGAAGGTGGGAGACCAGGGATATATGGCATCTTGCTCTGACACAGTCAGATCATTTTCAGTCAAAACAACTTTTTTTTTTAACTATATAATTAGTTGTAGAATCATGAGCTGGACCCAGCCTTTCTCGGCACCTACAGGACACCACAGGATAAAGAAAGCTAGCTAGAAAGGACCACGATTTGTCCTGTTCTGCCTGCTATGCTCAAGTGGCAAAGGAGACAATGGTGACCAGTGAACTCTGCTCCATGCTGGCCCTTCTGGGACATAACACTGACCTGTAGTATGGGAGAGGTTGGAGAGTCTTGGGGACAGTTTGGGGTGGGGGCAAAAGATGGGGTTACAAGTGGCAGTTAGTTCTTAGAGGAGGGTCTATACTGTTGGGTGTTTGTGTGGCCCAGATAATCCCATTTATTACATTTATTATATTGGTAGTCTAGATAATGACTACATTTTATACACTAATGAAAACACATTTGGACAGAGTGGGTACACACTGAGTAGGCCATTTTTTGTTTTAGGGCAGTGGTCCTCAGCCTTCCTGATGCTGCAACCCTTTAAATACAGGTGAGCCATCCTGATACTACAACTTTTTAATACAATTAATGTTGTGATGAGCCCCAACCATAAAATGACTTCATGTTGCTACTTCATACCTGTAATTTTGCTACTGGTATAAGTTGTAATACAAATATCTGATATATATATATATCTTGTAAAAGGGTCATTTAACCCTCAAAGGGGTCAGGACTCACAGGCTGAGAGCCACTTTTCTATTCCTTACCAACACCTTTCAAAGCTGTTCCGGTATGGGATAATTTTGAACATTGTCTTTTGGCTGACATCCAATATTGTCATATCCATACATGCATCTTACACTTTTGTTCCTCGCCTCTCCTGGCAGGTCTGCCTGAGCAGACACTGAAGGACACATGGTTTTTATTAAAAATTACTTCCCACTACTTTCCCTAGCCACAGTTGACTCCTGATGTGAGCTTTATCTATCCTATAATATTCTCTGCTGTTTTAATTTTCTCACCTGCCTGCCTCACAGTTTATATCAATGGGGTGTTTCTCAGCTCTGGGGTGTTTCTCTGAGACAGGCCACCACTGTCCCTGTGTCACAGGTGTCCCCCAGACACATCCTGGGCACACATAAAGCACAACACAGTTATGTCATTAAGGTGAAAATCTGTTCGATAGGTTTCAACCCTGATATATATATATATATATATATATACATATATATATATATATATACACATATACATATATATGTGTGTGTATGTATATATATATGTATATATAATGTATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTTTGTGTGTGTGTGTGTATACACACACACACAACACACATTTTTTTTTTTCTCCTGAGACAGGGTTTCTTTGTATAGCTCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGTCTTCGAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGTTGGGAGGGAAGGCATAGACCACCACTGCCTGACCTGATTTGTTTTTTTTTGTTTTTTTGTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTGAGACAGAGTTTCTCTGTGTAGTCCTGGCTGTCCTGGAACTCGCTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGTGTGTGCCACCACGCCCGGCTTTTTTCTATTTTTTTTCCTGATTTTTTTTTTTAAATACAAATATGAGAGAACCTAGATTAATTTCTGCCTGCGGAGCCAAGTTTATCTCTCTTTGGATAATTTGGAAGGTTGTTTTGTCCTGAACTTTAATCTATATAATAAATCTTATTTCCTTTAGTTAATGTGCATACTATTTGTTTTGACATCATTTCTAACGAAATTCGATCTAAAGGGAAGGGTCTCTAATAGTTCTATGGTTTGGGATTAAATGGAAGCAGGGAGGAAGAAGGAAGGGAAAGTTACCTATGCCACCTGTGTAACCAGCATAAAGCTCTCAGCACAGGTGCGAGGCTCTGGGTATAGTGCCCAGCTCCACACAAATAAATGAATAAGAACAAAAAACTTAAAGCAAGAAAGAAACCTAAACAGTCCAAAACTAAAGCAAAGAGGTACATGAGCCAAGCTTTAAACCACATAACATTGGTTTCAGTTATGCTCAAAAGTTAGACAAAGCAGGGGTTGGTTTGCTTTGGGGGGGGGGTGTTTGTTTTTTTGTTTTTGTTTTGTCATGGTTTGGCCTAGGTTTCTAGCATCTTTTCACCTAACCTAAGTCCTATAATTTAAGCAGAAATAAGACATCTTTGACCCTAATGTGAAGTCGCAAACACTCTTCAATTATATCTGTTACTACCCTCAAATGTGTTCCCTAAAGGCTCTTTCCCACTACAGCACTAGAACCACACAGTGCCACAGAAGGTGAATGCAGCAGAAGACCAGCACAGCCACATGCAGTGACTGCTCACATCAGAGTAAAGGCCACTGAGCATAGCAGCCCCTTCTGGGCGGGGTGGTGTGCACCTGTGATCCCAGCACTCCAGGGACTGAGGTAGGAAGGTCTAGAATTGAAGGCCAGCTGTGTAGTGAGACCCTGTCTCAAAACAAACAAACAAACAAACAAACAAACAAACAATCTCCTCAGCTTCAGCAGCATTTAAACATCTGTAGAAAGCAATGGTTAAGCCAGGCATGGTGGTGCACACCTTTAATCCCAGCACTTGAGAGGCAGAGGCAGGAGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAAGGCTATAAAGAGAAACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAGGAAAAAGAAAAAAAAAAAAAAAAAGAAAGCCATGGTTGCCTCAGTGAGATAGCTCGCCTTGGTCCAGTCTCATGGAGACATTTCTGAGCAGAGGGTAGGTCACAGCAGGCCTTCCCTCCTACCTGTGTGCATGAATTCTCTTTCAGAGCTGTTTTGTTTACTGAGGGCTCAGCAGTAGCTCCGAGAGGGGGGAAATACTGGTGAGGTGAACTTGTTTTAACATCAGGATACAACTGATCAAGATATTGGTTTGCTTTGCTGGGGGAAGGAGAGGAGGGCTCTGGCGGAAATGGCCTGTGCAGTTTGGCATTGGTGCTTTGATCGTTCCTTTTGTCTCTTTCCAGATTGCCAATGACGCCTGACACCCGAAATACAGGTATGAGGACGACCTTACAGAGTTGTTCTCTGTACACTTCCCCCACCACACACAACAATTGCTAGTTTGGTCATGAGGTCAGTAGTGCAGATGCCATCCTCACACACCTTAGCTTGAGCACCTGAGCATCCTCTTTGGTCCCTGGCCCCTGTATCCTAAGCATCAGCAACTGCATCCTAAAGTAGTTTTTTATTAGCGATGAGGTCTCAGAATTTGCTTCTGGTTGCAATGGCCCTTGAGAAGCTAGCAAACCTGTAGAAGTTGCAAAGACTCAGAAGCTCAGAGATATTCTAGAAATCTTACTGCTTTGCTGTGTATGTGGCTGTAAATAATCCATGTGTGCGTGTGTGTATGTGTATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATACATATATAATGTGTGTGTAAATATGCGTATATATACTTATATACACATATATGTACACATACATGAGTTGTATCATATATAGGTAGTAAAGTTATTTAACTATTATTTCATTATATTGTTACCAAATCACTGATACTTTTTCCATTTAAACTGTCAGACAACAAACAAGTTAATGTCCAGAGTATAAAAGCAGACATCCTCCCTAAAGTGGAGAAACCTTGTCTTTTTCACAGTTTTCTTTAAATACACACTCTAGCTGCTCCATAGGTTTCTCTCTTGATGCCCAATGCTTAGGTAATAGGTTCTGATACTACACGTAGGTTCTAAAGTAAAATTCTCACATTGGCTCAACATATAAAACAAATGTTTTGGTAGAATTTATCTTCTCGTATATGAAAAGTAGAAGTGTTGTATTTAAGGAGAGAGCCCTGACAGCCACATAATTGACAACTGAAGATCTGTCTCACCAGGGCCGAGAAAGGGGTAACACAGGTCTGCTAAGGGCATCATCTTGGAGGGGTCCAATCCAGTTCCTCCCAGCAGCTCCACAAAGTCACCAGTCCCACCGCAGCCAGCCGCAGGTTTCTAAGAGAACACAGCAGCAAAGAGAGCAAGATGCAGGTAAACCTATCACACAGGTCCATCTGAGCCAGCACTGAGCAGTGAACACTCTTGTCAGCCTTCAGACTGTAACTATGCCTTGACTCAAACATCAGAGACTCCAGAAATAGTTTAAGGTGGGGGGAAAGGCTCTCAAGAATCAGTGTTCTTTGTCAGTTGATATGTTATTGTTCAATGTATGTGTACATGCAAAAGCTAAATTTCATGACTGAAAAAATTGAACCAGGAATTTGTAAGCTTTGTAAGATGTATGGACACACACCAAATGACTGGGACCTCTTAATGCATGCAGTCAGAGGAAAGAAAGCCCAGCCACCCGCATTTTATAATTGCATTATAACTTGTTTATTAAATTTCTGTATTCTCCCTCAGCTTACATCTTTTCCTCTTGCTAACAGAGATATTTCTGTTCTTGGAACATGTCTTCCACAAATAAGGATTTAGATTACTCATGGGTATAGTTAAAAAAAAAAAAGACTAGAACTGTGATTTGGGGCTCAAGTTAGCCAAATGTACCATTTACTCTTTCCTTTGAATGGAAACAAATTCTTTTCATGGCACTCAAATGAATTCATTCTGAGCCTCTGAAACCATCCCTCCTTCCCTGCATCCTCCCACAGCTCTTAGCCACTGTGATTTCTGTGTCTCGCTCAACACAGAGAAAGTCTGGGTTCGTAGTCCCGTATGTCTCACTTGGCACCTGTTACACATGCCCAGGTTAGCCCTGATTTCATGTCAGTTCCCCCAAAGGGCCAGGATTATAGGGATGGGCTGCACATGTACACTAAATTTTTAATGACGTTTTAAAGCATTTCCCACATTTTCATTCTCTATGGTCTCCCATCATCCCAGTCGCTAGGTTTGCCTGTGACCCTGTTATTCACTGCTGGGTACACATTAAGAGATCTAGTGGTATGTGGCTGGTCAAGTTAACCCTGACACATGGGAGCCAAAATCTAAAACCGAGGACTTTAAACATCCACCCACTCCGTGCACCTGTCAGACATTGGGAAGAACACTCCAGTGTGGTAGGAACTAAAATGGAGACATCGTATCACGCAAACTATTTGGCTAAGGAATGGAGACAACAGGGTCTGCTTATGTTTAAAGAACTTACTATGAGCATGACTGTATGCTTAGGTACTTCAAACGTAATTTCATTACATGATGAAATAAAGATTTAATTAGGCCCACTGTGCACAAGTTTGTGAACAGACATTCAAACAAGCTATTTAGGAGTGGAAGAACAATAATTGCCCTTCCCATGAGTAGATAGACGGAAGTTTCCACTTGGTGAGTGATGCCATCTCTCTCCTCTTTGGAATCCTCTCAGGTGTTCTTGTAAACAGTGGCAGAAAGTGAGCTGCATGAGGACCTGTCTGGGGTTCCGGGGCCTCAGCAGCTCCCGCGTGTCTTTGTTGAAGCGTTAAGCTCCTACCCCCTCTTGCTAGATTGTGACCCTGTGCCAAAGGCTCACTCCAGCTTGTTGAACTGGAAGCCAAATCCCTGCTGTTCGTTCTGAATGTCTGAAGTCTCACCCCCAGCACACATGGAAGCTGAGGTGCTTATGGGATGATCTTCAGTGGTTCACTAGCACAACCATAGAGTCTTCTCACGGGGAACGTGAGGCATCTCGCAGCATGTCCCCCAGCCCATTCGTCAGGTGGAAGCCAGAATGACCTTCAGGACAGCACACATCAGGTCCTTTCCCACCCAACCCCAAAGGCTTTCCCACTACTCCTGCTGCACACAACCTTCTCCGGGTGGGCAGCACACGGGGCTCTGCCCAACTCCCTCTCGAGCTTGCTTCTTCCTGTGTCTAGGATCCAGTAAGCCTGTTTCTGCCAGGGCAGCACTGTTCCATCCACTCCTCCTGCCTCCTATGGTTGCTGATTCTTCTAATCTAAGTTTCCTTTCCTGACCACACTGTCTCAGGCAGTTCCGTCTACCCACTTTCCAGCTCCTGTCTGTCTATCAATCCACTCTGCTGCATTTTCTGGACAGACTTACCATGATTTGAAATTCATGATAATTTTGCCTGTGCCATCCCACTTCAAATAGGCTCCCTGTGTGAGCAGGACCTGCCTTGTTCATTGCTTATTTCTAGAACCAAGAATAGTCCCTGAGCAAAGGAAATTATTATTGTTGTTGTTTGGCAGTACTAGTAAAGGGAAGGTCATGCAAGCCTGTCACAAGAAGTTCTCCTATCTCAGTCTCTCAGTACTGGGATTACAAGTATAAACCACCATACCCAGCTTTCAATACCCAGGTGTCAAAAAATAGGTTTTCAAAGGAATAGATGCCTAGAACACAGTAGGCACAAAAAATATTAAGGGAAATATAAAAGTATTCTTAGAGCTTTCATACCAAAATCACAGATTTCCTAATATGTTACCATGTATCCCACCAGATTCTAGATCTTTCATCAAGAATAAAATGGCTTACGAACACTGTCAGGCTGGATCCAAAGCACTCAGACATTCTTATCAAAGGATCGCTCCTCAATCAAAGTCCAAGGGCAACAAAGAAGAAGAAGCAGGGACACGTGGGTGTGGGGGCTGGAAGAAGTTAGATATCAATTCTTTCTCTCGGTCTTGTTCCTTATTTAGTTTTCACAAAGATCTGTATGTTTGGGAACACCGGCTGCCTCTGATACCACCAAGTGCACGCATTATCTTAACTTAGGCCGATGAACAAGGAAAAGGAAATGAAGGTGACTCATGGAGCAGCCATTGTCTTTGCAGAGGGATGCTGTGAAGCCAGCATTCATTGTAAGGCACACACGGAAGCTGATATGAAAGCAAGTTGGTTGAAATTTCACAGCATATATATATATACATATATATATATGTATATATATATGTATATGTATATATTAGACACACACATACATATATACACATATATAAATACATATACACATATATGTATATACGTATATGTGTATATGTATATCTGTGTGTGCACATATATATTAAGCAAGATGTCATATAACCTGCTCTTCAAAACTTTACTGCTTTTTGCAATAGAAAAAAATCCAATTTCATATTTTATGCCACCGTTAGCATTCTTTCTTAGCCTAGTACTGTCAATGAGAGTGACTTCCTTCTTATCAAAAGAAAATTCCAATAATCAGGAGAGCAGCTGTGTCGATGACTCAGAGGAGGCTCAGCTAGGAGAGGATTTTTGTAAAAGAGGAAAGTACAAGAGTGTTCGATTGTAATTACATGCTGCAGCTTTACCAACTGAAAGCATTTCCTATGTCACAAGAGAAATCCTTGAGTAGTCAGAATGGGCACCTGAGTGTTTGCCTGCAGCGTGACTGAAATAGACATTGAGGAAGCCAGGGCATCGTGCAGAAGACTTAAAAGTCAGGGGCCACATGCTTCCTGACAGGATGGGTTATTTGTCACTGATAAAACAAAACAAACCAAACAAACAAACAAACAAACAACAACAAAAACAACAGTGGCCTTACCAAATGGCCAAGGAGCAAGCAAGCCACTGACCTTTAACTGAAGGCCATGCAAGTGTCCGAGGGTAAGATCAGAGATTTTGATTGCCACTGGATAGATGATGGAGAAGCTGCAGTTTTGGTGCTGGTATGGAACCACTAAAGTAAACCTTCCACTCGGAGTCTGAGAGATGACATTGCAAGCTTAGGAGGTAAAAAAAAAAAAAGATACAAATACATTATTTAGAGTGCTGGACTTTTTAAAATAAATTATTTATGCCTTTGCTCTAGATTTCAGTTCATGGTCTAGTAGGTATATCCAACCTGGAGCCCAATGGCCACATGTGTCCCAGGATTGCTTTGAATGGTGCTAAACATATTTGTAGATTACCAACATTCAAGGGCTGGACCTCTCTATTTAGGTGTTGGGCCCAGGGAGAGGGTATATATCACCATGACAATCTGGGGAGAGGAAGGAGCAGGGGACTAGAACTCAGTGACATGTGAATATTGTCCGGAAGTGCCAGCCACAAGAGGGCTTCTCACCTTTGTCTGTTTATAACAATCCTAAGGTAGGAAGTCTTTATCAGTCAGACAACATACCTGGGGGTACCAGGAATACTTCTGTTGAGTTGATTCTAGATCCGAACTGGGAGAACAAGGCTCAACCACGAAGTCAGAACCTCACCCATAGAATTTGAAGCCACGGTGTTGTTTGTTAAACAATAAAAACCATGTCTAATTTTATTCAAAAGATTTCATGATACTGACAGAAGAAGTGGAGAATAAAATAGACAACAGTTTTATACCTAATACATATTTGCTGGGTTTAGAGTAATGTAAAGTTGACTAAGTGGTCTTTTTTTAAATTTTTATATGTATTATTGTTGTTGTTGCTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTAGAACTCACTCTGTAGACCAGGCTGGCTTCAAACTCAGAGATCTATCCGCCTTTGCCCCTAAGTGCTGGGGTTAAAGGTGTGGGACACCATGCCCAGCTTTAACCTTTAAATCGTGATAGTCTTAAGATTTTCTGAAATTTTTCTTTTTATTTCAAGTCTAATCTTACAACTGAGTTATTCTTCTGTCTGTGAAACAGAGGCATGGGATTGGCCTTTCTGCTGACCCACCGCCTTATGTTTTTAATTACTCTTAATTTCAGAAATAAGTATTTTTTAATGACTCTTAAATTCAGGAATAAGTGAAATTCCTTGACAGTTTAAAAAGACATCTTCTGATTTTTTTACCAAGCAAATGCCCACAGAAATGAACCCCCAACATTCTGTTTCTCAGATAGCCTCAGCTTTTCAGGGCAGAATTTCTTTCCCTCTCCTTCCTGTTTCCCTCTCTTCTCCCTAGCTTCTTCCCTCCCTAGTGCTAGAGTTTGAACCCAAGTTTTGCTTCAAGCATTCTTAGACAAACGGTTTACCACTTAACTACATCCCTAGTTCTCAGCTTTTCCCCTTAATGAGGAAATGAGCCGATGTACGAACTCGTTCTGCTCAGAATCACGCTCTTTCAGGCTGGCTATCTAGTTTTCCAGCCTAATCTATGTTTCTCTCTGTCAGAAATGACACAAGCTGGGGTGGTGGCTTTGTGGTAGAAGGTAACGTGGAAGGAGGTGATGGAGGGGCACTGGTTCACTCCCTCCTTAGGGGACAAGACAACATCTGCCCACCTGTCTCAGACTTTGTTTACAAAGCTCAGGCACCAAATGATCCCACAAGCTTATCCCGAAT
GF3 -1 -1 1 GTTCTGCAATTAGATTCTAATCTAGTCCTC
GF4 -1 -1 1 CAGTTCTGCAATTAGATTCTAATCTAGTC
EX52 -1 -1 1 GCCATGGTCTTCTTCCGGGTCCACG
EX32 -1 -1 1 GACCTGATGCTCCTTCTGGTAAGACCACTC
GR3 -1 -1 1 GAGCCAGCACTGAGCAGTGAACACTCTTG
GR4 -1 -1 1 GTAATAGGTTCTGATACTACACGTAGGTTC
Comments:
Created
Target: 2803Ensembl ENSMUSG00000021680 -- 96212023 -- 96212233 ENSMUSE00000119961 -- ENSMUSE00000119961
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:13:1:120284312:1, start 96212022: end 96212233
Found 5 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:13:96217733:96218732_746 G3:13:96207523:96208522_202
ACCEPT (0) gap pair G5:13:96217733:96218732_746 G3:13:96207523:96208522_63
REJECT (1) gap pair G5:13:96217733:96218732_746 G3:13:96207523:96208522_197
ACCEPT (0) gap pair G5:13:96217733:96218732_157 G3:13:96207523:96208522_202
ACCEPT (0) gap pair G5:13:96217733:96218732_157 G3:13:96207523:96208522_63
ACCEPT (0) gap pair G5:13:96217733:96218732_157 G3:13:96207523:96208522_197
ACCEPT (0) gap pair G5:13:96217733:96218732_12 G3:13:96207523:96208522_202
REJECT (1) gap pair G5:13:96217733:96218732_12 G3:13:96207523:96208522_63
ACCEPT (0) gap pair G5:13:96217733:96218732_12 G3:13:96207523:96208522_197
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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