High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 178923
Chr: 8
Strand: 1
Status: Ordered
BACs: RP24-498A5 : RP24-545E5 : RP24-303I8 : RP24-232P11 : RP24-430L15 : RP24-228E17 : RP24-232D9 : RP24-502J8 : RP24-200L18 :
Instance: Plate 143: Well D03: BACs RP24-232P11 RP24-545E5 RP24-498A5 RP24-303I8
Oligos on GRCm38:
Type Start End Length Seq
G5 95083623 95083672 50 ACCTGGCTCTTTCACATTCCTAAACATTATTGACGACTCCAAAAGGCTTT
U5 95089487 95089536 50 ATGGGCTCATCCAGACTCCAGAGCAAAGGTTCAACATCTCACCTTGATTC
U3 95089597 95089646 50 CTAGAAGGGACATGATTGAAGGATGCCTGCCTGGGGTTTGGGTTGAGTTT
D5 95090205 95090254 50 ATATCACGTTATCAATGTAGCAGCAGTGGTCACTGCCATCTCCCAGTTGC
D3 95090297 95090346 50 ATGTACATCCCTGTATTGAGAACCCAGAAGGACATCAAATCCTAGGAGGT
G3 95094261 95094310 50 TTAACATGTAACAAACTGCATACTTGTAATTTCCTGGAGTCTTGGGAGAC
Oligos:
Type Start End Length Seq
D5_D3 97614155 97614196 42 TGGCATGAGGTGTCCAAGGGATGAAGAGTAAGGTGTGTGTAC
G5 97607523 97607572 50 ACCTGGCTCTTTCACATTCCTAAACATTATTGACGACTCCAAAAGGCTTT
U5 97613387 97613436 50 ATGGGCTCATCCAGACTCCAGAGCAAAGGTTCAACATCTCACCTTGATTC
U3 97613497 97613546 50 CTAGAAGGGACATGATTGAAGGATGCCTGCCTGGGGTTTGGGTTGAGTTT
D5 97614105 97614154 50 ATATCACGTTATCAATGTAGCAGCAGTGGTCACTGCCATCTCCCAGTTGC
D3 97614197 97614246 50 ATGTACATCCCTGTATTGAGAACCCAGAAGGACATCAAATCCTAGGAGGT
G3 97618161 97618210 50 TTAACATGTAACAAACTGCATACTTGTAATTTCCTGGAGTCTTGGGAGAC
G5_U5 97607523 97613436 5914 ACCTGGCTCTTTCACATTCCTAAACATTATTGACGACTCCAAAAGGCTTTCATTTATGAAAGTCACACTTACCCATATATTCTGTTAGAAAACAGTACTGAGACAGATGTGCACTCAAACCTGACAGGAGTGACGATGTCATCACACGTGTGTCTTCTCAACGGCTCCTCTGGAGCGAGTGAGAGCACCCAAGGCAATACCATCCTAGCATTTCAGACAGTAGCTCCGTTCTTTCGGATCCTCTAAAAGGCTCTGGAGGATCCCTAGATCAGGCCTGGAGGACCTTCATCTCACCTCTGTGGCTTTCTCTCCAGCCGTAGCTGGTTGTCGTAGGTTTGGTGTGCTGTCCCCGGGCTGTTTTCAGGAGCAGATGAGGAGACTTTGGGCAAAGCTGTTTTGGTGACTCAGTCTCCTCATTGAGAGATTGAGATCATTTTTAAGTTGTCCCCCAAAGCTGTAAGGTTCTGGGAGGACCCTGTTCATGGCAACTTTTGGCAGAGTAGCTTTATGTCAATTGGCATTATGCATCTTTAATGGCTTACCCTACCTGAAGGTAGAAGCTGATGCGAGGGAGCAAGTGAGGGCCAAAGTATCCAAGGTCACTGCATAGTGGGAGCTCAGCCCGGAGAGAGCCACAGCTGTCTGTTTCCAGTCCTGCTCTAATTCTGAGTGTGCGTGCTTGCATGTGCTTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTCAGTTAATTACTGATGAGGGTAGAGGGTGCATGAGCAGCGAGTGCAAAGGCCCGAAACAGGAAGCTTTATATGTTGGAAAGACTTACAGGAAATCACAGAGCTTTCACACATCAGCAGGTGAGAAAGGGTTACCATGGAAACCTAGTGAGTTGTGCAGGGGCACATGTCCACAAGACCCTTGTTACTAAAGATCTAAGCATCAAAGTGTTTGAGCAGCAGGCAGTAGTATGATCAGGTATGTTGAGGATCTGAGAGTTTCTGGTGCACAAAGGTTTTGGGGTATATAGCCTGAGGCCACACAGGCTGGGGCTTCTCGGGGCTTCAGGGTGTGATGAGGCTCAGAAGACTCTTTAACCAGTGTAGGGTTGCTGCACCGCTGTCCCCTGTATCTTCCTCCTAGGATGTAAGTCCTTAGCACTTGTGGGCAGACAGAGAGTGATCCTCAGTTTCGGCTGAGGTATGCCAGTTCGGCTGAACTGCTGGTGTCTGGGGAGGTGCTGTTAGCTTTTGCTGGAGGAAGAGCCGCAGCCGTCATCTGGTCTGTAGGAGACATTGAGGAATTTGATTTGTTTGCATAAAAATCCAGCACCCACACGTGGGCCATTGCTGCTCGGTGTTCTCCCATTCAACCCCCCACTCCCTGCCTGTGACTCTGCCTGTTCCAGGCCTTGCCCTGGATCCATCTGATGCCCCTGGAGCCTAAGGGCAGGAAGCTCTCCTTCCACAGCCTCCTGTGCCGGCCTAGAGCAGATGTCTAGAAGCCTCTTCTGTTAGACCGCCACAGCTGTCTCTAGAGGTGGCCCTGAGGCCTGTTGAGATGGGGACTCAACCAGATCTCTCACTCTCTGCCTGGGGAGAGCCACGGCAGATGCCCGGGGACTCTCAGACAAATTCAGGGTGTGGCTGCAGAGACAGGGGCTGAGCTACTCAGGAGCAGCTGCATTCCCCTCCTGCTGGGCTGGCCACATATAGAAGGGTCTGTGGTCCAAGCTTGTGGGCAGCTGTCCCCAAGTGTGCAGAGGACAAGACTCTAGTTGAGCTGAGAATTAGAGCAAGACCCTCCTCCATTGGGTTCAGGTGGCAGGCCCTCCAGTACCTAGATTGAAAGCGTCTGGGCTTACAATGGGGCTCTCACCCTGGTTGGGCCAGTTGATGCTCGTGCCAGCAGTGCCCTGCCAGGCCCTGCAGGGAGGTGTCATTTCAGAAATGAACTGTCCGGCTTTCCTGGCCCTGTTTTCCAGGCTCCTTATGGTCTGCAGTTCATACAAGAGCTACTCCAGGCTGGGTAGCACAGGACCTGGGAGGAGGGGAGGACCCCTTACTAAAAGCCATGGCATTCCTTCTCCGTGCCACCCAAGCCAGTTTCCAGCTCCTTTAGGGAACCTGCATGTTGGGGCTTCCCCTTCCCCTCTCCTTTCTACTTGTTCCCAGAGGAGGTTGGAGACCAGGGACTGGCTTGGAGGCCTAGTCTGCCTGTTGATTCTCGGCTGAGTTTGTTCTTTCTGGAGCACTGATAAGGTCCGAAGTTCCCACCTTTGCTCCCCACTTTGGGCCTCTCACTGTGGTCAGGCCTGGGGCCCTGGTGGACTGTCCACAGGCCTTATCTCCTATCTACAGGGCCAGCCTTTTCCCACCCAGCTCCTGCTGTGTCTGCTGTCTCCTGCCATTATCTCTTTGGAACCTACTCCCTGCCTACCTGGGACCCTGATGACCACTCTGTCCCCATGGCTGGTCTTCTGTCTGCATCTCAGCCCATTCCGAATGACCTGGCCCTGGGGTCATCCTGTTCTCCACACAAGGACTCTCTCTCACTCATGAATGTCCAAGCCCATGCAGCTGTCAGATTAGCACCATTTCAGGAATCTGAATTGGGACCTAGGGACAGCCATCAAGACAGAACTTGAATTCTTCTCAGTGTTTGTCCCAGATGGGCTTTGTCTCTATGGATGGCACCTGAGCACTTGAAAAGTGTGGTGTATCGGTCCATTTTTTAAAAATTATTTTTAATGTTTTTTTTTTTGTATCAGAAAAAGTATTATGAGTATATCTGGATATTAGTGCTTAAAATAAGATAGATAAAAATGGGAAACCAGTTATTTATTTATTTATTTATTTAGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTATAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCAATTCCTGGGATTAAAGGCATGCGCCACCACTGCCCGGTTTGGAAGCCAGTTTTTAAAAGTGAAGTTAGTCTTCGCACAGTATCTGTGGTAATCGGTTCTAGGCCAAGTCTGTAGATGCTCACTGCTTTATGCAGTGATGTGGTCTGTGTGTGCAGAAACGTGCAGCTCCTCTGGGTACCTTGACTCACCTCTGTTACTTGCGATACTTAATATAGTGCAAGGTGGTTTGAGTATTTCTTGCATTGTATCTTTCATGGACTAGCAAAGAGGAGAAGTCTCCGTGCACTGATACCTGCAGGTGCTTGTTTCCCAACAGGGTCTTGCATGTGGCTGAATCTGGCCTGGATCTCATGATCCTTGTGCCTTCGCCGCCCACGTGCTGAGTTTACAAATGTGTATAATGTGCTCAGTATAACGGACGTGCCTGTCCCAAGCATTCCCAGCAGCAGCTGGCTGAGTGTGTGTGTATGGAGGATGTGCTATGCAGTCTGTGTGGCTAGCTGTTGGGAAGAGAAATGAACTCAGGAGGAAGTGTCCTAGCAGGGCAGGCTAAGGTGGAGAGACAGTCACCTCAGCCTCCGGCCCGCACTTTGCCTTGCTGCTGGTCCTGAAACTAGTGGGGGTTACCTGGGTGTCCTGCTCAGAGCTGGTGGCTAAGCATCCTCCATGTGGCCTGTCAAAGTCCCCTGACCACTTTTCCTCCCTCCTGTCTTATACAGAAGAGATCGTCGCCCATGCCAGCAATGTGTCCTCCTTGGTGCTGGGCAAGGCCTCAGGGCGGCTGCTGGCCACTGGTGGGGATGACTGCCGAGTCAACCTGTGGTCCATAAACAAGCCTAACTGCATCATGGTAAGTGATGACGTGCCCCGCCCTTGGGGGGAGCGCCTCTGCACACCAGCAGTGCTACCCCAGTTGTTGATCACACACCATGTACAAGGCTATAGGCTGCTTTCCCAGGCAGGAGGGAAAACCAGTAATTTCAGGCCGTTCTGAATGACCAGGTTTTAGTCTGCACCTTTCTAGATACATGTACACACACATGTATCTCGGAATACTACCTTACTCTAGTGTGACCTGGTTCTTTAGAAGCACAGTGTCCTGAGGATTTTCCTACGTCTACAAATCTGGTTTACTGCATGCTTTTACGTGGCTTCTTTGGTATCCACCACCGGCATGAGCTGTAATTAGCTTAGCCACACCCTGTCATCAGTAGGGCTGCTGGCTCCAGCTTTTATTTTCTGCTAAACCACACTATGTTGATCATTCTCATTTGTCCAAACCTTTGAGCAGGCCTGGAGTATGCAGTTCTGAGAAGCTGTCCCAGCAGGCTCCGGGAGTCACTTCACATTTTCACCCGCAGCATGGGAGCATGGCTGTGTCCCGTGTCCTCTCCAGACATCTTAAGCAATTGTCATTCTTTTGACTTTTGACCAAGTAAACAAACAAACAATAAATGGGGGACGTTGATAGCTGTTAGTGAAAGCTTGGTTATTTCAGGGTTTTTTGTTGTGAGACGGAGTAAAAGTGAGACCACTCAGATCAGAGCCGGTACGTCCAGGTTCAGTAAGAGGAGAATCGCTTTGCTGTTTGTTGGTTTTCATTTTTATTGTTATCCTTTTTTATTTTTGTATTTATTTTATTTAATTGGTGTGTGGCTAAGGGTGGGTGTTTTGCCTGCATGTATGTCTATGTATAATGTGCATGCAGTGCCCGTAGAAGCTGGAAGGGGACCCTGGATCTCCTGGAACTGGAGTCACAGATGAGTGGTTGGTAGCTGCCATGTTGGTGGTAGGCATTGATTCCAGGGCCTCTGGATGAGCCGCTGGTGCCCTTAACCACTGAGCCATTTCCTGAGCCCCTGGTTTGGTTTTGTTTGTAGGACTCAAACGTTTTCCTTCCCCACAAAATGCCAAAGTAATATGCATAAATTGAAAAATAATCAGCACAGGCTGGAGAGGTAGCTCAATGGTGGAGCTTGCCTAAATAGGTTTTTAAAAAATAAAAAGTCACTAAGAGATAAGAAGATCAGTGCTGAAGTATTTCTCCCTTCAATGACTGCTGGGTCCTCTCTGGAGATAATCAGGCCCCCTTCTGTGTTCTGGAATTATTTAGTCCATGGACAAGAAAATACATCTGGTTTCCCTCCAGAGTCTCATTCTGTGTGCACTGCGGTAGCCCTGTGGAGCTCAGTCCTCCTCGTGTTTGAAGGGTTACTGTGGAGGACAGTTACATGGACTCCCAGTCTCTAGCCAGGAGTAACCTTGGGTTTGTGCTTGCAGAGAATAAGGTCCCTGAAGAATTGTGGCGTTGTTGTTTTTATGAGACAGGGTCTTAGTGTGTAGCCCAGGCTGGCCTTGAGCTTGGAGATTCTTCTGCCTGGGAGACCAGGGATGGTCCTTACCTCTGAGATCTCAGGTGTACATCAGCACACCCAGCCTTGGACTGTGGGTTTGAGGGGAACATGCATTCCTTATGGGGTTGTGTTTGCCCAGTTGTGATCCTGGAAGCCTGGGCCCATGTAGACCCCTACCATTGTAAACAAGCTGCTACAACTACAGGTTCAGGCACTGGTGCTGAGGAACGCCCCCGTTTCCCTCATGTCCCCGTTGGGAATATTGCCTTAGCTAACACTGGTGAAGGGGCTGACAATGAGCAGAGAGTGGCAGACACCTTGGACTTGGGGTCCGGTCTTCCCTTCCAAAAGCTGAGGCCGACAACACCTCCCACCTTACCTGGCCCAGCTCTCCTTGCTCTGTGCTCTAGACATGCAAGAACCCACCTCCAGACCTTCCCAGGCCTCTGGAGCATGAAGCAGGCACCCCCGGGAGTCAGGTACCCAGCAGTCAGGCAATGGAGATGCATCCCTAGCTTGCTATGGCCTCTACGGTCAGAGTAGACAGAGACGGGGAGAGCCTGGATGGGCTCATCCAGACTCCAGAGCAAAGGTTCAACATCTCACCTTGATTC
U3_D5 97613497 97614154 658 CTAGAAGGGACATGATTGAAGGATGCCTGCCTGGGGTTTGGGTTGAGTTTGGCTTATTTTTTTCTTTTTTCTTTCTTTTTTTTTTTTTTTTTCCCAGAGTCTCAGTATGTAGTCCAGACTGGCCTGGAACTCATCCTCCTGCCTTTGTTTCCCAAATGCTAGGGTCACAGGTGTCTGAAGGGCCGCTTCATCCATTGTGCTGATGAGACACCATGCTCACCTGAGTTAGGATATAGGCGGGGCCCCAGAGAACAGCTCACCACATATTATTTCTTCCAGAGCCATTCTCAGGGACCCTAAATCCGGGGCAGTAGCTGGGGGTTACCCCTGCAGTTCATTGGAGCTGCCACCTTCTCTCTGCAGAGCCTGACAGGTCACACGTCCCCTGTGGAGAGTGTCCGTCTCAACACTCCTGAGGAGCTCATCGTAGCTGGTTCCCAGTCGGGCTCCATCCGCGTCTGGGACCTGGAAGCTGCTAAAAGTAGGCCTTCCTCCTTCCTATCCCTTTGTGTGTGGGTCCCACTTCAGGCTTGGGTCATTGGGCTGCCACCTCCAAGAGGTCTGGGTGCTTTGATCCCTGATCACTGAGCAGAGTGGGTGGTGCCTCCATATCACGTTATCAATGTAGCAGCAGTGGTCACTGCCATCTCCCAGTTGC
D3_G3 97614197 97618210 4014 ATGTACATCCCTGTATTGAGAACCCAGAAGGACATCAAATCCTAGGAGGTAGCCTGCAGGACATGTGAATAAGTAGACTGCTTTGTGGGAAGAGGCAGATACTGGGGAGCTCCCACAGGGGTGGGGGTGGGTGGCTGAGCTTCTGTTTGCTTCTCTTCCCCTTGTCTTCAGTTAATCTTTCACTTTGACAGGAATAACAGAGTTCTGAAAGTTTATTTTATCATGAACCTTGATGGCACAAGGCTCATGAAGGCTTCGACCCAGTCATCCCCGCCCCTGTTCTAAGAGCCTCCAGTCTCCCTTCCTTGCTGTTGTGCCCACACCACCACCATCGCTTTCCTAAAGACACAGCATTTCCCTTTAGAACAACAAAACAAAACCAGGTGGGCCCTTCACACGGTGCCTGCCTAGAGACCCAGCACTCAGGAAGCTGAGGCAGGAGAATTGCTGTGGAGTTAAAGCCCAGCTTAGCCTGTGGTGTAAGACCCCCCCCCCCCAACTTCAAAGAAACAAACAGGAAATCCTCAAACAAAAACTGTACCCTCCTGCAGTGAGAGTCATGGGGCATTGGGCTGCCACCTCCAGGAGGTGGACTTGGCTCTGAGCAGCCACACCAGGTCTGAGCCCACTGGCATAGGCTCTACTGCATAACAGTAACCCCTGGCATGTGTGCCCGGCAGTGACCTTAACCAGGAATGGTGGTTTTGTGTTGTGGGCCCTGGAAAAGATGGCATCCTTTAGTCCTCTAGAGTGACTGCATGTTGATGATGTAGTAAGCACAAAGCCCAGGACGCCCTTATGACAAACAGCTGTGCGCTGTGTCTCAGCAGATGTTTTTTGCCCAGGCCACATCAGGCCTCAACATTTTGTGCTTATCACTGGACATGGCAGGCTCTGTCCCAGAAGGCAGGCAGTCCCCAGGGAATATCACCACCCCTCATGCTTTGCCAAAGAGCCAAGGGCCAAAGAGCCACAGCCCACCCCAGACTTCCTCGGCCACTGTGCTCCTGGGAGATGAGAGTGATGCCTTCATATCGCAAGGTGGAAATGGACCATACAGCCTGGTTAGCAGGCCCACCATTCTGATAGATGGGCAGACTGCGACTATTGAAGCACAGGGCTTAGTGGAAACCCTGAGCTGTAGGTGGCAGAACTCCTGTCCCCCACCCTCGCCCCGTGCTCTCCGCTGTCTATTACTACCTCAGTTGATGATTCTCAAGCTTGTCCTCAGAGGGAGAGCTAAGGTCTCAGGGCCACTGCCATTCCACTGGGCAGAAGAGATGAGAATCCAGAGAAGCTTCTGAGAGAGCCACAGTTAACCGTGGAGGACGTGGACAGAGGCTGAACAACCACTCCACTGGGAGACCTTGGAGGTCCTCACATGTGCAGACAGGTCCACGTCTCTTCCACTCTGCAGTCCCTAGAGCCTGTGACCAAGGCTAGGTATCCCCTGGAAAAGACAAGTAAGGGCAGAAGTCATGGCAGAAGAATGTGGTCCTGCCAGCTTTCCTTGGTGCGGCTGGAAGGGAAGGGCGCAGGCTTGCTGGATCCCACAGGAAGGCACCCACATCTCAGGAGGCTGGCCTCTGTCAGGTGGGCCCCACCACAGCCTCTAGTGTCTGAGTGTCCCTTGCTGTGGCAGCAAGGCAAGAGGGGCAGCATCCTGAACTTGTAGCAATACAAGACAGCTAAGGAGCAGGGAAAGCCAAGCACTCGGTGTGGGGTATAGCTGAGTTCGACATCCATGCCGCAGATGAGTCAGGTGCCTCGGCAGAATCCTCAGGCGTGAGAGGGGGTGTCATTCCAGCTGCAGTGCCCATCAGAAACCATGTGCGGAGCCAGAGGGACAGTGGACCTGGGCCCCAGCTGATGGCTGGCTAGGACGGCTGTTGCCTATGTGCTGTTACTGCCAGGGCAGCTCTGGCTCCTGTTGACTCCAGAGGCCGCCCTTGTTCTAATTGTGGCCATCCTCAGGCAGGGTGCCTTCCTCTGAGCCATCCTCCAACCACCTCCTAGCATTGTCAGAGGGACTCAGAGCTAACATGGAGTCGGCCAAAGCTGAACACCAGAAATGCTCAGTGGATGTGCAGTGATGGGAGGCACCCGATGGCCACAGCTCTGAGTGGTGCGTCCTCTCAGGACAGAAAAGGGAGGACACTTGTGCTCCGAGGTTGGGGAAGAAGAATGAAGGTCGAGAGGACAGACTGGAGGACGTGAAGGCGGCCTAGGGAGCAAACCAGAATGGGACCTGTACCGTCCCACTCTGAGTATTTGGTGTTTATTCTCTCAGTGTTTAGTTTAGTTTCGTTGTAAACCAATCTGAAGGTCAGAGAGCATACGCGGTGGCCTAGAGTCACACAGCAGTTAAGAAGCAGAGCTGGGACAGATCTAGCTTTGTTAGGCTGCCAAGCTCATTCTCGTGTAGATGGGCGCATTCTACAGTAGCTACTCAGTTCTCTGTTCTTGGATCTCTGCATACCTGTCCCTAATGTGGGAGCTCACTCCCTGTTTAACCTTGGCATAACCTAGGGATTGCTAGACTCTGTAGCCTTTGACCTACCACTGGCTGGTAGGCTTTGAAGTGTAACGTTTTTAAAAAGCAAGACAAGAGATAGACTTGAACGCTGCCAGGAATACTCTTGAGACACAAGTTTGCCACCCCGGCCTGCCAGACCTTAACGCGGCAGCAGGACAGCAGTGAATGACTAGTGTGCGACTGTCCTCTGCTCTCACCTGCCTTAGGGCCATCTTCAAGGTCACTTGCATAGGTACATCCCACTGGGCATTGTGAGTCCCCCTGCATCCTCCTCTTGCCCATGACTGCCATCCAGCTGATGCTGTCCCTTATGTTCCTGGATTCTTTAGCCAAGCAGCTTCCCTGATTGCAGCCCCTCTCTGCTCTCTCCTCGGTGCTGTCCTGGGGGTTAGTCACCCTGTAGAGTAGCCCCATGGCATGTGGCCTGGCAAGCATTGTGATTGATGCTGCCCGGGGGCCATGGTACACACAGGCTACGGGGAATGCCTTGTGTTTTTCTTGGGGCATGAGTAGTGATGAAAATGGCTGTAAGACTGTCAGGGTGACAGGGCTCTGGTTGGTCTCCTGTTTTCACATCGGCATCTCAGTTTCTGTGCCAAAGGGTCCACACAGACGGGTTTAGCTGCCTTCTGCTGGCCAGCTAACACTTAACCTGGCACTTCTGCTTTATCTTGCTTCATTAGTTCTTCGAACACTCATGGGCCACAAAGCCAACATCTGCAGCCTGGATTTCCACCCCTATGGTGAATTTGTGGCCTCAGGCTCCCAGGACACAAACATTAAGGTGAGGTGCCACTCAGCCACCCATCTCCCAAAATCAAGTGTAAGGGAATCTTCCCCTCGTGGCAACATCCTTACCATCCTCGGGACAGAGGACATGTGGACGCTGGCCAGACACTTCAACTTGGAATTCCCAACTTGGGCAAACCTCCAACCTTTCTCTTTGGGGCACATGACCTAGAGGTATCCAGAGTCCTCTCTGCTGAGAGCTGTACTATACCCTCTGACCTAGCCTCCAGAGACATAACAACTTCCAGTGGTTCACCCTTCCTACCCCCACCTCTGCTTCTCTCCTCTATAGCTCTGGGACATCAGGAGGAAAGGCTGTGTCTTCCGATATAGGGTAAGCATGGTCTGTGCTGGTTACCAAGCTGGTTACCACACAGAGCTTGGGTCCACACTACAGTGGGGCTGGGGGCTGCAGTCTATTGCAGGTGACTCAGAACAGACCTTGAGTAGGATGAAGGGCCTTGGGTGGTCTAGCTGGACCGCCACCCCCCCAGGCTTCCCACCAACCCCTCGAGTCACATATATTAAACTGTCCCATGCTCTGGTGTCCCCGGGTGCCGGGCTTCCTGCTCTTGTGTATGTGCAGTACTGTGGCTAACAGAATCCTTAAGGGCTTTTGGAAGAGGCCATAAAACACCTTTAACATGTAACAAACTGCATACTTGTAATTTCCTGGAGTCTTGGGAGAC
U5_15 97598388 97613436 15049 CTCATCTGAGGACCTTTCCCTTGCAACGGCTCTGAAAATCATCCCATTTCCACCAAAATGAAAACCCTATCAGATGTAACTGGAATGCCCAGAGAGTGGGGGTTGGGCACACGACTAATATTCATGTGTACTTCAGAACATCCCTCACCCTGGGCCTGGGGTTGGGTTCCAGAGCCTTCCTCATGGTCCCTACCTGCACCTCTGGGGATCCTAGACCCCAGATCCCAGGGACCTATCCTGAAGGTGACAGGATATTCTTTCTTATGCCCTAGACTCTAGGATGGTCTCGCCTTCCTCTTCTAATAGCATAACCGTAATCCCCCTGCATCTTTCTTTGGTCCCTCTTTGTTATTATGTTTTTGTAGAGAAGGATCCTGGGGTCTGGAGAGGTTAAGCACCTTGCTTGAGGCCACACAGCTAGAAAAAAGGGAGTAGATTTAAACCAAGTGGTCCCTAGACCTGCCCTGGCGCAGCTTCCCTGTCACCGTGGTGGTTCTGGTCTCAGGTAGAGCCGATGGAGCACACTAAGAAACTCCTCTACCAGTACGAGACCATGAATCAGCTGAAGAACGAAGAAAAGCTGTCCCGACATCAGGCCTGGGAGTCGGAGCTGGAGGTAAGGTCCTACCCCAGACCTCGGGGGGGGGGGGTGGCGGGGGGGGGCAGGGAGAGAGGGCCTCCGCCGTGATGCACCTCCTCCATGATGTCTGATCAAGGTGCTGGAGATTTTGAAGCTCCGTGAAGAGGAGGAGGAGGCACACACGCTGACCATCTCCATCTACGACACCAAGCGCAACGAGAAGAGCAAAGAGTATCGGGAGGCCATGGTGAGTCTCCCGGCTCTTCCTGGGTCTCTCTCCCCGGAGCCCTGCCTCCAGGGTAGGGAGGATGTGAGAACCCTGTGTAAGGGATGATGAGGGGATGTGCCAACCCTTCACAAGGGATGATGAAGTCGCTGGTGATGTCAGGTGAGTATTCCTGTTGGCTATCACCTGCTCGTTGATGGGTCACCCTCCCACCCCCATCCCAAAGAGCACTTAAGATCCAGGCTGGGTGTACTTACCCAGGACTGTGACCAAATGAAAAAGTCTCTACCTTGTGTAGAGAAAGAATGAGAACTACATGCCCACCTCTACCAGGGCTTTCACTGAGGCTGTGACCTGAGCTGGGAATCCAGCCAACTGACTCAGGCACAGGTTGAACACCAAGACTCAGCCACGCAATGCAGGGCTTTGGCTTTACCCACTCTCGCCAAGTGGGAGGACCAGTTTGGGTTCCCAGGCTCGAGGCTAAATCCTCTTCATCAAGCAGTGCAGATCTCTAAAGAAGTTTGGAGTTGGCCTGGCAAGGCTTGGCTTTGGAATGTTCTTGGATCTATAGGAAAGGGCAACAGCTCCTGTCCTCATGTTCTCTGGCTCCTGTGTGCCCCATGTAAGGCCGGCCTTATGGGCAAAAGAGAAAAATGGGAGCTCAGAATCTTTGCTAACCCACTGCCACTCACTATAAGTGTCTGCTTTGGACTTTCCAGACCTGAGCTGGTCTGTTTTCAGAGGAGGAAACTAAGGCTCAGACTGATTGATGAATTCGTGTCAGAAACAGGAATTACATGTTAGAAAGAAATAAACAGGGAGAAGGAGGCAATCGTGTCTCATAATCCTGGGACATGGATTTAGGGCCTGGCTTTTACCATTGCACAATGGACTCAGCTGCTCCGGAGGTCTCCTGCATATCCCGATCTTTCAGCTGGTTCCCTGGCCCACAGGAGCGCGTCTTGCATGAGGAGCACCTGCGGCAGGTGGAGGCCCAGCTGGACTACCTGGCCCCATTCCTGGCCCAGCTCCCTCCAGGCGAGAAGCTAACCCGTTGGCAGGCGGTGCGTCTCAAGGACGAGTGTCTCAGCGACTTCAAGCAGCGGCTCATCGACAAGGCTAACCTCATCCAGGCTCGCTTTGAGAAGGTGTGGCCAGGGCCCAGGCAGGGGAGGTGACCTCACCATCCTCAGTGAGAAAACGGGACCTGAGGTCTTTGCTCAGTGCCTCACGGATCCGAGAGTGACAGATGAGCGGATGAGTGTGTGTCTGTGGCATCCAAATACCTGGTTTCTTCTTTCCCCTTTGTTATTGTATGAGTGGATGGATGGATGGTGGGTAGCTGGGTAAATGGATGAGTGGATGGATGGGTTACCAGGTGGTGGTGGGGTGGGCAGGTACAGTTGGATGTGCAGTGAGGATTAGAAGTGGAGTGTGGGTGGATGGTTGGTTGGTTGGATGGATGGATGGATGGATGATGGTATGGATGGGTAACTAAGATGGGCAGACAGGGGGATACAAGAGTGGATGGGTAGGTTTGTGAATGGATGGACGGTTGGATGGTACATTATAATAGCTCCTAGTCCTTGGCCTGTTTCCGTGAGACAGGTGAGGTGCGTACCACGTGGTCCCCACGCAGTGTCTTGGTCTGCAGAGTGCAGAGAAGAGTTGGGTGGAATGGGACCCACCTTGTTTTCGGAGACGAACTGCTGGGGGCTCTGAGCACGCCCGCAACATGTCTAGCTCATGTAGCTATTTTGTGTGTATGGGTGTTTTGTCTGTATGTGTGCATGCCCACCACATGCATGCCTAGTGCCCTCAGAGGCTAGAAGAGGTCATCGCATCCCCCGGGACTGGAGTTTCAGATGATTCTGAGCCTCCATGTGCATTTTGAGAATCTAACCCCAGTCCTCTGGAGAGCAGGGAGTCCCCTGCTTCATTTTTTGAGACAGGGTTTCACTCTTTGGCCTTCACTGACCTGGAACTTGCTGTATAGACCAGACTGGCCTTAAACTCACAAAGATCCATCTGCCTCTGCCTAATGGGAGCTGGGATTAAAGGTGTGCACATCCCACCCAGTTGTTTTGATTTTATATATGAGCTGCTAGGAGTGGAACCCAGGGCCATGCACTAAACCACATCCCTAGCTCTCGCTGACTGTTTTGGCCCTGTTCTGATTTCATGCACATGCTGTCCTTTAAATCATGACCCCAGCAGTTTCCACCCAAGATATTTTGGGATGCTCTAACTGTGTCCCTTTCCTTGGGGGTCCGGGTTGGGGTGTTATCCTCTTGAGGCTGCCTGATCATCTATGGAACTACAGCCTGGCTGCAGATCAGTTCGCACATTCCCATGTTCATCTCTGTATGCTTCTAGGAGACCCAGGAGCTGCAGAAGAAGCAGCAATGGTACCAGGAGAACCAGGTAACCCTGACGCCGGAGGATGAAAACCTGTACCTGAGCTACTGTTCTCAGGCCATGTTCCGCATCCGAATCTTGGAACAGCGGCTCAATCGGTAAGGGGCAGAGCCGGGAGGACTCCTGGGAGCACTCTGATAGCTTGGATATCCCTCACCTTCTCCTCCCTCCCTCTGCTCAGGCACAAAGAGCTGGCTCCACTGAAGTACCTGGCGCTGGAGGAAAAGCTCTACAAAGACCCACGCTTAATAGACTTCGTAAAAGTCTTTGTTTGATGGCCCCTCCTGGAACTACGGCAGACCCTGCCAGAGAATGGAGTCTCTCCCTTCTCCCCAACCACTATGATCCTATACTAGCCTCGCTGCCACACTACCTCCCTATCCCTCATAGCCTCCCTCAAATAAATGCATTTCCTGTTTGCCATTTATACTTCAGTGTTTACAAGCTCCAATCTACACCACCCTAAATCTTTTTCTGTCATCTTCTCTTCTGGATTATCAAGTGTTGGCTCTCCAGATGGTTCCATGGTCACCTGCCAAGATCTGCTGAGAGCTCAATGTGTTGATGGTGGAGCAGCTCCTAAAGTGAGAGCGGCTTAGCTAAGGAACACACCCCAGGACCAGGCGGCTTGATATTAGAGGACAAGAGACAGAATCCAAGTTCCTCTTTCATTTACCACCTGTGAAATGGGGCTATCCATGTTTCCTGAAGTAAGACTTAAAAAGCAGAGAAAACTGGGTATAGTAGCCCATGCCTGTAATCTAAGCAGGAGGATCAGAGTTTAAGATCTGTCTCATCTACATAAGGAATCTGTTTCAACTACATGGGTCACATGAAGCTCTGTTTAAAAAAAAAAATAAACAAAAAATAAAAGGGACTGGAGAGATGGCACTGACTGCTCATCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATAAGATGCCCTCTTCGGGTGTGTCTGAAGACAGCAACAGTGTATTCATATACACATAAATAATTAAAACAAGAAGGAGGAGGAGGAAGAGGAAGAGGAGGGGAAGGAGGAGGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAAAAGAAAAGAAAAGAAAAGAAAAGAAGAAGAAAAGAAGGAAAGAAGAAGAAGAAGGAGAAGAAGAAGAAGAAAAGAAGACCTTGCCTGTAATCTTCTCCAGGGCATGACACACTCAAGACAGGTCTCACAGCGGCTGTGGTTGCCTGTACTACCTGGGCCAGTGGTTAGGCAATCATGTATCAGGGAGGGACTCCTGGGGCTCTCTTCTTGTTGAATTACTGGCTGCTGCTGAATTCCGGTTGCGGGGCAGTCATTGTTTTCACTCAAAGATGAGCCCAGTAAGCTTTAATGCATAGTTCCAAACCCATGGTTAAAGCCAGGGGTGCATGCTCATGTGGTAGCCCATGCCTTTAATGCCAGCTCTTCATCTCTGTCAGTCCAAGGCCAGCCTGATCTACAAAGTGAGACCCTGATACAGAGGGAGGAGAGAAGAGAGAGAGGAAGGGTAGTGGGAGATCCAAGAGACAGAAATAGGATGATCTTAGAAGAGTCAAGTATGCCAAGAGTGCAGAATCTTGTCTGGTTCCTATAAAGATACTGTGCCCCGCCCCCCACATGTGTGGTGTGTGTGTACACATGTCCCATTGTATCACTATGCATTGTCCTCTGACATTCTTCACCTTATTCCCTCGAGTCAGGGTCTCTCACTGAACCCGTAGTTAGGATGACAACCAACAATCTTAGTAATCCTCTTGTTTCTGCTCCCTATAACACTGCTCCTTATAAGCACACATGCACCATGCCCAGATTTTTACATGGGTCGTGAGGATTTGAACTCAGGTCTTCATTCTTGCACTGCAATCACTCTTAGCCACTGAGCCTTGCCATTTCTCTTGGTCCTAGGTAGTTTGTATTTCCAGTGCTGGGGATTGAACCTAGTGCCTCCTACATACCAGTCAAGACTCTCTTATCACTGAGCTACATCCCCAGTCTTGTTCCTGCTTATAAGTTATGTGTATTGGGGATGGTGACAGTCATTTTAATATAGTCTGCATGTTAGAGAATGAAGTTATTTCAGGGGTGGTGACATTGGTTATGTGACTAAGGGCCCTTAACGATCTTCAGGCTCAGTGTTGCCATGACTGAAATGTCTTAGGATCTGTAATGTACATATAATTGTATAAACAAAGCAGATAAGGCAAAATGTGTCAAAATGAAGAATTTGACTTTTTTTTTTAAATAAAAAGTTGCGGGAACAGCAAAGTACAAAGTTTTGATCAAATGTTATTCTCCAAATCCAGGTCTCTTAATAATTACCCATTTCTACGCAGGGCCGACTTCCCATTAGGCTGGTGGGTGACTTTTGTTCCCGTAAAGGTTTTGCATTTAATAGGGACTTTGTGTGCCTTGCCTTTAAGAACAAAAGTTTAAGTCCTGGGTCCGATCTTCCAAATGTCTGGTCTTTTTCACCACAGCATCTGTTTCCTCGTCTAGAAATGGGAAATTCCTCCCGTTCCGGACTGTGTAGAGGAGAACGCCAGATGCAGCAGCCCCGACCAGAGGGGAGTGAGTGTAGGATAGACCAGAGTTGGAGGTTATTTCTGCAAAGGGCTGGGACCGAGTTTCTCAGTAAGGGGGGGACCCGAACTCATGGTGGGTTATGTAGCCTGTCCCCATCCCAACCTGTCTGTCCTGTCACAGATATTAGCCTTTCTCCGGTTCCGTAGTTTTACTTCCGCACGCAGGCGAGCAGACACAAGCCTAACTCATCCCACTGTGCTACTGTGATCTTAGGGACGAACTCCTGGGCTCGACATCGACCCCACTGTAACTAAGCTCTGTTTCCCTCGAGGTCAGCGAACTCCAGGAGACGTGTGGATCCAAGTAAGAGCCAAGTCCTCCCACTACGCGGGCGACACTCACAGTCTGAGGGCGGGGTCCTGGCAGCCCTGTTTCAATATGGAAGATCTGCAACTTCATACCACTCTAGAAGTCCCCTTTCCATTGGCTATCTCAGAGCTGGTTCTCCAATAACACTAACGTTTATTGGTCAATCCAGAGCTGTTGCTGAAGTGATTGGCGCAGCCGCCATCGGGTGCAGCTCGCGGATTTTTTAAACACAACCGGGTTCCAGGAAATGCAGGAAAAGGGCGGAAAAAACCAAGCCTGACTGGGGGCGGGACTAAGAACGCAGAAGCCTGTATTGGTTCTTCTGGGACCTAAGGGCGGTCCGTAAGCGCTTCTTTCTCGTGATTAGTCTGAGTCGGGGAAAGGAGGCGGGTAGTTACGTAAATCGGCGGAAGCGCTTGCTTCCATTGGTCAATTCTAGAGGAAGGCGGGGCGTCCGTGGCGACTGATTGGTGTAAGAAGTGAAGTGGGAGGGACTCTAGGCCGGAGGTTTTGTTGTGAGGGTCGGTTGTCAAGCAGCGACCAATCAGGGCAAAGGATGGAGGCAAGTGGGGGTCGCGCCTGGAGGGAAGCCTTGGCCTCCAGAGCTACAGCTGCAGGTAGAGTGGCTCTCGGGGTCTTGGAGGCAAAGGGAAAATGGCTCTAGTCTTCGCCGAGCCTGGAGGGGACAGTCCGGTCGCCGGCAGGAGGCTCTCGGCCAGGACAGGCTGGAGCCAGTGCCCCTCAAGGCAGACGGGAGGAGGGTCAAGCGAAGCTGAGGGACCGGTGGCAGGTTTGGGATGAACGAGCAGGACGGGTGTGTGGAGGCCGGAGAGGGAAAGCTGGTGGGCCGGAAGCAGAGCTGAGGGTGTGCGGAGGATCGTATTGAGTCGGGGCTCCAGAAACAGGAGAGCGAATGAATGGGGGATTCTGATAGCAGCGGGGCAAGAGAAGTAAAGAGGGTCTGGCGAAGCATGGCCAACTAAGGAAGCTCAAGATGTGCCAGAACGGGAGCATAGGCAACAGGAGAAAGAGAATCCAGCGAGAGAGCTCCTGGGCAAGAGGCGAAGGGCCGTGGCGGCGGTGGAAGGCTCCATCCACGCTGTGGCCTTTTAGAATTGAGGAGTCATTCCGACTCACACCTAGACTGGAGACTCAATTCCTAGCTATGCCCTTGAGAGTTGCAGGGAGTGGGCGTGGTTCCCTTACCAGGGAGTTGGTGATGGAGGAAGCAAGAAGATTGGACCCTCAGAATCCTCTCAACATCACCCCCTCCCAAGGCCTTTACTGTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGAACGTCTGCCTATCTCTCAGTCTCTCTGTTCGTCTCCGTGTATCTCTCTGTCTCTCTGCCTTTTGGATGCCTCTCATGGGCACCAAAACATAACCTCAGACTCCAGTGCCCCATCCAATACATGGGGTGCAAATCCAAGTGGACTGCAGTTATGTGATCCTCTCTGCCCCATAGCAGGTGCTGGGTTGGCAAGTTAGGACACCCAGCATCCAGGAGCATTACTGTAGGCCCCTGAGAAGTCAGTCTCTCCATCCCCTCTCCACCTACCTATGTCAGTCTGTCCTTCCTTCCTTCCTTCCTTCTTTTCTTTGAGATAGGAACTGACAGAGATCTGCCTACTTCTAGCTGGCCTCCTGAGTGCTGGGGTTAAAGGCGTGAGCCACCAGGCCCAGCTACCTAGGGAAGGTCTTTTATCTTTGGGCTCTTAAGTCACAGGTTTTCAGACCCAGTGTGCACTGCAGCTCCAGGCCCTGGAGGCTCTGGCTAGTCTGTATCAAGTGGCCTTGATGACCCAGGGACTTGTAACTCGTCTCAAGCAGTTCCTAACCCACCATCGCCTCTGCTTGCATTCTAGCCCAGTGTTGAGCTGAGATGGTCCAAGCCTGACATTCCATGATCTGGGATCCCGACCTATGTGTCCAGGCTGCTGCTTGATAGCGGATGATCTAAACCACCAGGCAGTCCTGGGCCAGGAGAGGCGGACTCTCTGCCTGGATGTGTGGGAACTCCCTGCCAACTTGATTGTCGGATCTGGGGGGGATCCACCCCCACCCCACAGAGAAAGCACAATCTGTTTTGTGGGTGGGGTCTCAGGCGCCACCCCCTGAAGCATGGCCACCCCTGTGGTCACCAAGACAGCCTGGAAGTTGCGTGAGTACTTTTCATTTATCACCCTGTTGTTTTGTAAGCTGAGTAGTCTTAGCCTTCAGCTCTGTCTCCTCTCCCTGGCACAAGAGAGACTCACAGTCCTTGTTTTCAGGTAGACAAAGATGTCAATCACATTGGGCAGGTGATTAAGCAACCCTTCCATACAGCAAGATAAGCACAGCTGTGCTTGGTACTTGCTCTGAGATTCAGGTGAGATCTGTCTGGTCAAGCTGAAGAGCGGAGTGTTGCGAGGAAGGCTGAAAGGCGGTCCAAGAACCCCAGACATGTCCCAGGTCCCTTTTCCTCCATCAGATGCAAGGATCTTGGGATGTGGATGGAGGGGAGGTTGCCCAAAGATGCTGGCAGGGCTCTGAAGTGAAATCAAGCAAAAATAGTTCAGAATTCTTTAGCCTAAACTCTCTTAGATTCTTTTCCTTTGTTGAGATAGGGCATCCCTGTAGCCTTTGCTGGCTTGGGACATGTTCTTCTGCCTCCTGTGTGCTGGGATTAAAGGACTGTGCCACAATGCCTGGCTCTTTCATTTTGTTTTTTTGTTTATTTGTTTGTTTGTTTTTCTTTTTCTTTTCTTTTTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGCATCACCACTGCCTGGCACCTGGCTCTTTCACATTCCTAAACATTATTGACGACTCCAAAAGGCTTTCATTTATGAAAGTCACACTTACCCATATATTCTGTTAGAAAACAGTACTGAGACAGATGTGCACTCAAACCTGACAGGAGTGACGATGTCATCACACGTGTGTCTTCTCAACGGCTCCTCTGGAGCGAGTGAGAGCACCCAAGGCAATACCATCCTAGCATTTCAGACAGTAGCTCCGTTCTTTCGGATCCTCTAAAAGGCTCTGGAGGATCCCTAGATCAGGCCTGGAGGACCTTCATCTCACCTCTGTGGCTTTCTCTCCAGCCGTAGCTGGTTGTCGTAGGTTTGGTGTGCTGTCCCCGGGCTGTTTTCAGGAGCAGATGAGGAGACTTTGGGCAAAGCTGTTTTGGTGACTCAGTCTCCTCATTGAGAGATTGAGATCATTTTTAAGTTGTCCCCCAAAGCTGTAAGGTTCTGGGAGGACCCTGTTCATGGCAACTTTTGGCAGAGTAGCTTTATGTCAATTGGCATTATGCATCTTTAATGGCTTACCCTACCTGAAGGTAGAAGCTGATGCGAGGGAGCAAGTGAGGGCCAAAGTATCCAAGGTCACTGCATAGTGGGAGCTCAGCCCGGAGAGAGCCACAGCTGTCTGTTTCCAGTCCTGCTCTAATTCTGAGTGTGCGTGCTTGCATGTGCTTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTCAGTTAATTACTGATGAGGGTAGAGGGTGCATGAGCAGCGAGTGCAAAGGCCCGAAACAGGAAGCTTTATATGTTGGAAAGACTTACAGGAAATCACAGAGCTTTCACACATCAGCAGGTGAGAAAGGGTTACCATGGAAACCTAGTGAGTTGTGCAGGGGCACATGTCCACAAGACCCTTGTTACTAAAGATCTAAGCATCAAAGTGTTTGAGCAGCAGGCAGTAGTATGATCAGGTATGTTGAGGATCTGAGAGTTTCTGGTGCACAAAGGTTTTGGGGTATATAGCCTGAGGCCACACAGGCTGGGGCTTCTCGGGGCTTCAGGGTGTGATGAGGCTCAGAAGACTCTTTAACCAGTGTAGGGTTGCTGCACCGCTGTCCCCTGTATCTTCCTCCTAGGATGTAAGTCCTTAGCACTTGTGGGCAGACAGAGAGTGATCCTCAGTTTCGGCTGAGGTATGCCAGTTCGGCTGAACTGCTGGTGTCTGGGGAGGTGCTGTTAGCTTTTGCTGGAGGAAGAGCCGCAGCCGTCATCTGGTCTGTAGGAGACATTGAGGAATTTGATTTGTTTGCATAAAAATCCAGCACCCACACGTGGGCCATTGCTGCTCGGTGTTCTCCCATTCAACCCCCCACTCCCTGCCTGTGACTCTGCCTGTTCCAGGCCTTGCCCTGGATCCATCTGATGCCCCTGGAGCCTAAGGGCAGGAAGCTCTCCTTCCACAGCCTCCTGTGCCGGCCTAGAGCAGATGTCTAGAAGCCTCTTCTGTTAGACCGCCACAGCTGTCTCTAGAGGTGGCCCTGAGGCCTGTTGAGATGGGGACTCAACCAGATCTCTCACTCTCTGCCTGGGGAGAGCCACGGCAGATGCCCGGGGACTCTCAGACAAATTCAGGGTGTGGCTGCAGAGACAGGGGCTGAGCTACTCAGGAGCAGCTGCATTCCCCTCCTGCTGGGCTGGCCACATATAGAAGGGTCTGTGGTCCAAGCTTGTGGGCAGCTGTCCCCAAGTGTGCAGAGGACAAGACTCTAGTTGAGCTGAGAATTAGAGCAAGACCCTCCTCCATTGGGTTCAGGTGGCAGGCCCTCCAGTACCTAGATTGAAAGCGTCTGGGCTTACAATGGGGCTCTCACCCTGGTTGGGCCAGTTGATGCTCGTGCCAGCAGTGCCCTGCCAGGCCCTGCAGGGAGGTGTCATTTCAGAAATGAACTGTCCGGCTTTCCTGGCCCTGTTTTCCAGGCTCCTTATGGTCTGCAGTTCATACAAGAGCTACTCCAGGCTGGGTAGCACAGGACCTGGGAGGAGGGGAGGACCCCTTACTAAAAGCCATGGCATTCCTTCTCCGTGCCACCCAAGCCAGTTTCCAGCTCCTTTAGGGAACCTGCATGTTGGGGCTTCCCCTTCCCCTCTCCTTTCTACTTGTTCCCAGAGGAGGTTGGAGACCAGGGACTGGCTTGGAGGCCTAGTCTGCCTGTTGATTCTCGGCTGAGTTTGTTCTTTCTGGAGCACTGATAAGGTCCGAAGTTCCCACCTTTGCTCCCCACTTTGGGCCTCTCACTGTGGTCAGGCCTGGGGCCCTGGTGGACTGTCCACAGGCCTTATCTCCTATCTACAGGGCCAGCCTTTTCCCACCCAGCTCCTGCTGTGTCTGCTGTCTCCTGCCATTATCTCTTTGGAACCTACTCCCTGCCTACCTGGGACCCTGATGACCACTCTGTCCCCATGGCTGGTCTTCTGTCTGCATCTCAGCCCATTCCGAATGACCTGGCCCTGGGGTCATCCTGTTCTCCACACAAGGACTCTCTCTCACTCATGAATGTCCAAGCCCATGCAGCTGTCAGATTAGCACCATTTCAGGAATCTGAATTGGGACCTAGGGACAGCCATCAAGACAGAACTTGAATTCTTCTCAGTGTTTGTCCCAGATGGGCTTTGTCTCTATGGATGGCACCTGAGCACTTGAAAAGTGTGGTGTATCGGTCCATTTTTTAAAAATTATTTTTAATGTTTTTTTTTTTGTATCAGAAAAAGTATTATGAGTATATCTGGATATTAGTGCTTAAAATAAGATAGATAAAAATGGGAAACCAGTTATTTATTTATTTATTTATTTAGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTATAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCAATTCCTGGGATTAAAGGCATGCGCCACCACTGCCCGGTTTGGAAGCCAGTTTTTAAAAGTGAAGTTAGTCTTCGCACAGTATCTGTGGTAATCGGTTCTAGGCCAAGTCTGTAGATGCTCACTGCTTTATGCAGTGATGTGGTCTGTGTGTGCAGAAACGTGCAGCTCCTCTGGGTACCTTGACTCACCTCTGTTACTTGCGATACTTAATATAGTGCAAGGTGGTTTGAGTATTTCTTGCATTGTATCTTTCATGGACTAGCAAAGAGGAGAAGTCTCCGTGCACTGATACCTGCAGGTGCTTGTTTCCCAACAGGGTCTTGCATGTGGCTGAATCTGGCCTGGATCTCATGATCCTTGTGCCTTCGCCGCCCACGTGCTGAGTTTACAAATGTGTATAATGTGCTCAGTATAACGGACGTGCCTGTCCCAAGCATTCCCAGCAGCAGCTGGCTGAGTGTGTGTGTATGGAGGATGTGCTATGCAGTCTGTGTGGCTAGCTGTTGGGAAGAGAAATGAACTCAGGAGGAAGTGTCCTAGCAGGGCAGGCTAAGGTGGAGAGACAGTCACCTCAGCCTCCGGCCCGCACTTTGCCTTGCTGCTGGTCCTGAAACTAGTGGGGGTTACCTGGGTGTCCTGCTCAGAGCTGGTGGCTAAGCATCCTCCATGTGGCCTGTCAAAGTCCCCTGACCACTTTTCCTCCCTCCTGTCTTATACAGAAGAGATCGTCGCCCATGCCAGCAATGTGTCCTCCTTGGTGCTGGGCAAGGCCTCAGGGCGGCTGCTGGCCACTGGTGGGGATGACTGCCGAGTCAACCTGTGGTCCATAAACAAGCCTAACTGCATCATGGTAAGTGATGACGTGCCCCGCCCTTGGGGGGAGCGCCTCTGCACACCAGCAGTGCTACCCCAGTTGTTGATCACACACCATGTACAAGGCTATAGGCTGCTTTCCCAGGCAGGAGGGAAAACCAGTAATTTCAGGCCGTTCTGAATGACCAGGTTTTAGTCTGCACCTTTCTAGATACATGTACACACACATGTATCTCGGAATACTACCTTACTCTAGTGTGACCTGGTTCTTTAGAAGCACAGTGTCCTGAGGATTTTCCTACGTCTACAAATCTGGTTTACTGCATGCTTTTACGTGGCTTCTTTGGTATCCACCACCGGCATGAGCTGTAATTAGCTTAGCCACACCCTGTCATCAGTAGGGCTGCTGGCTCCAGCTTTTATTTTCTGCTAAACCACACTATGTTGATCATTCTCATTTGTCCAAACCTTTGAGCAGGCCTGGAGTATGCAGTTCTGAGAAGCTGTCCCAGCAGGCTCCGGGAGTCACTTCACATTTTCACCCGCAGCATGGGAGCATGGCTGTGTCCCGTGTCCTCTCCAGACATCTTAAGCAATTGTCATTCTTTTGACTTTTGACCAAGTAAACAAACAAACAATAAATGGGGGACGTTGATAGCTGTTAGTGAAAGCTTGGTTATTTCAGGGTTTTTTGTTGTGAGACGGAGTAAAAGTGAGACCACTCAGATCAGAGCCGGTACGTCCAGGTTCAGTAAGAGGAGAATCGCTTTGCTGTTTGTTGGTTTTCATTTTTATTGTTATCCTTTTTTATTTTTGTATTTATTTTATTTAATTGGTGTGTGGCTAAGGGTGGGTGTTTTGCCTGCATGTATGTCTATGTATAATGTGCATGCAGTGCCCGTAGAAGCTGGAAGGGGACCCTGGATCTCCTGGAACTGGAGTCACAGATGAGTGGTTGGTAGCTGCCATGTTGGTGGTAGGCATTGATTCCAGGGCCTCTGGATGAGCCGCTGGTGCCCTTAACCACTGAGCCATTTCCTGAGCCCCTGGTTTGGTTTTGTTTGTAGGACTCAAACGTTTTCCTTCCCCACAAAATGCCAAAGTAATATGCATAAATTGAAAAATAATCAGCACAGGCTGGAGAGGTAGCTCAATGGTGGAGCTTGCCTAAATAGGTTTTTAAAAAATAAAAAGTCACTAAGAGATAAGAAGATCAGTGCTGAAGTATTTCTCCCTTCAATGACTGCTGGGTCCTCTCTGGAGATAATCAGGCCCCCTTCTGTGTTCTGGAATTATTTAGTCCATGGACAAGAAAATACATCTGGTTTCCCTCCAGAGTCTCATTCTGTGTGCACTGCGGTAGCCCTGTGGAGCTCAGTCCTCCTCGTGTTTGAAGGGTTACTGTGGAGGACAGTTACATGGACTCCCAGTCTCTAGCCAGGAGTAACCTTGGGTTTGTGCTTGCAGAGAATAAGGTCCCTGAAGAATTGTGGCGTTGTTGTTTTTATGAGACAGGGTCTTAGTGTGTAGCCCAGGCTGGCCTTGAGCTTGGAGATTCTTCTGCCTGGGAGACCAGGGATGGTCCTTACCTCTGAGATCTCAGGTGTACATCAGCACACCCAGCCTTGGACTGTGGGTTTGAGGGGAACATGCATTCCTTATGGGGTTGTGTTTGCCCAGTTGTGATCCTGGAAGCCTGGGCCCATGTAGACCCCTACCATTGTAAACAAGCTGCTACAACTACAGGTTCAGGCACTGGTGCTGAGGAACGCCCCCGTTTCCCTCATGTCCCCGTTGGGAATATTGCCTTAGCTAACACTGGTGAAGGGGCTGACAATGAGCAGAGAGTGGCAGACACCTTGGACTTGGGGTCCGGTCTTCCCTTCCAAAAGCTGAGGCCGACAACACCTCCCACCTTACCTGGCCCAGCTCTCCTTGCTCTGTGCTCTAGACATGCAAGAACCCACCTCCAGACCTTCCCAGGCCTCTGGAGCATGAAGCAGGCACCCCCGGGAGTCAGGTACCCAGCAGTCAGGCAATGGAGATGCATCCCTAGCTTGCTATGGCCTCTACGGTCAGAGTAGACAGAGACGGGGAGAGCCTGGATGGGCTCATCCAGACTCCAGAGCAAAGGTTCAACATCTCACCTTGATTC
D3_15 97614197 97629247 15051 ATGTACATCCCTGTATTGAGAACCCAGAAGGACATCAAATCCTAGGAGGTAGCCTGCAGGACATGTGAATAAGTAGACTGCTTTGTGGGAAGAGGCAGATACTGGGGAGCTCCCACAGGGGTGGGGGTGGGTGGCTGAGCTTCTGTTTGCTTCTCTTCCCCTTGTCTTCAGTTAATCTTTCACTTTGACAGGAATAACAGAGTTCTGAAAGTTTATTTTATCATGAACCTTGATGGCACAAGGCTCATGAAGGCTTCGACCCAGTCATCCCCGCCCCTGTTCTAAGAGCCTCCAGTCTCCCTTCCTTGCTGTTGTGCCCACACCACCACCATCGCTTTCCTAAAGACACAGCATTTCCCTTTAGAACAACAAAACAAAACCAGGTGGGCCCTTCACACGGTGCCTGCCTAGAGACCCAGCACTCAGGAAGCTGAGGCAGGAGAATTGCTGTGGAGTTAAAGCCCAGCTTAGCCTGTGGTGTAAGACCCCCCCCCCCCAACTTCAAAGAAACAAACAGGAAATCCTCAAACAAAAACTGTACCCTCCTGCAGTGAGAGTCATGGGGCATTGGGCTGCCACCTCCAGGAGGTGGACTTGGCTCTGAGCAGCCACACCAGGTCTGAGCCCACTGGCATAGGCTCTACTGCATAACAGTAACCCCTGGCATGTGTGCCCGGCAGTGACCTTAACCAGGAATGGTGGTTTTGTGTTGTGGGCCCTGGAAAAGATGGCATCCTTTAGTCCTCTAGAGTGACTGCATGTTGATGATGTAGTAAGCACAAAGCCCAGGACGCCCTTATGACAAACAGCTGTGCGCTGTGTCTCAGCAGATGTTTTTTGCCCAGGCCACATCAGGCCTCAACATTTTGTGCTTATCACTGGACATGGCAGGCTCTGTCCCAGAAGGCAGGCAGTCCCCAGGGAATATCACCACCCCTCATGCTTTGCCAAAGAGCCAAGGGCCAAAGAGCCACAGCCCACCCCAGACTTCCTCGGCCACTGTGCTCCTGGGAGATGAGAGTGATGCCTTCATATCGCAAGGTGGAAATGGACCATACAGCCTGGTTAGCAGGCCCACCATTCTGATAGATGGGCAGACTGCGACTATTGAAGCACAGGGCTTAGTGGAAACCCTGAGCTGTAGGTGGCAGAACTCCTGTCCCCCACCCTCGCCCCGTGCTCTCCGCTGTCTATTACTACCTCAGTTGATGATTCTCAAGCTTGTCCTCAGAGGGAGAGCTAAGGTCTCAGGGCCACTGCCATTCCACTGGGCAGAAGAGATGAGAATCCAGAGAAGCTTCTGAGAGAGCCACAGTTAACCGTGGAGGACGTGGACAGAGGCTGAACAACCACTCCACTGGGAGACCTTGGAGGTCCTCACATGTGCAGACAGGTCCACGTCTCTTCCACTCTGCAGTCCCTAGAGCCTGTGACCAAGGCTAGGTATCCCCTGGAAAAGACAAGTAAGGGCAGAAGTCATGGCAGAAGAATGTGGTCCTGCCAGCTTTCCTTGGTGCGGCTGGAAGGGAAGGGCGCAGGCTTGCTGGATCCCACAGGAAGGCACCCACATCTCAGGAGGCTGGCCTCTGTCAGGTGGGCCCCACCACAGCCTCTAGTGTCTGAGTGTCCCTTGCTGTGGCAGCAAGGCAAGAGGGGCAGCATCCTGAACTTGTAGCAATACAAGACAGCTAAGGAGCAGGGAAAGCCAAGCACTCGGTGTGGGGTATAGCTGAGTTCGACATCCATGCCGCAGATGAGTCAGGTGCCTCGGCAGAATCCTCAGGCGTGAGAGGGGGTGTCATTCCAGCTGCAGTGCCCATCAGAAACCATGTGCGGAGCCAGAGGGACAGTGGACCTGGGCCCCAGCTGATGGCTGGCTAGGACGGCTGTTGCCTATGTGCTGTTACTGCCAGGGCAGCTCTGGCTCCTGTTGACTCCAGAGGCCGCCCTTGTTCTAATTGTGGCCATCCTCAGGCAGGGTGCCTTCCTCTGAGCCATCCTCCAACCACCTCCTAGCATTGTCAGAGGGACTCAGAGCTAACATGGAGTCGGCCAAAGCTGAACACCAGAAATGCTCAGTGGATGTGCAGTGATGGGAGGCACCCGATGGCCACAGCTCTGAGTGGTGCGTCCTCTCAGGACAGAAAAGGGAGGACACTTGTGCTCCGAGGTTGGGGAAGAAGAATGAAGGTCGAGAGGACAGACTGGAGGACGTGAAGGCGGCCTAGGGAGCAAACCAGAATGGGACCTGTACCGTCCCACTCTGAGTATTTGGTGTTTATTCTCTCAGTGTTTAGTTTAGTTTCGTTGTAAACCAATCTGAAGGTCAGAGAGCATACGCGGTGGCCTAGAGTCACACAGCAGTTAAGAAGCAGAGCTGGGACAGATCTAGCTTTGTTAGGCTGCCAAGCTCATTCTCGTGTAGATGGGCGCATTCTACAGTAGCTACTCAGTTCTCTGTTCTTGGATCTCTGCATACCTGTCCCTAATGTGGGAGCTCACTCCCTGTTTAACCTTGGCATAACCTAGGGATTGCTAGACTCTGTAGCCTTTGACCTACCACTGGCTGGTAGGCTTTGAAGTGTAACGTTTTTAAAAAGCAAGACAAGAGATAGACTTGAACGCTGCCAGGAATACTCTTGAGACACAAGTTTGCCACCCCGGCCTGCCAGACCTTAACGCGGCAGCAGGACAGCAGTGAATGACTAGTGTGCGACTGTCCTCTGCTCTCACCTGCCTTAGGGCCATCTTCAAGGTCACTTGCATAGGTACATCCCACTGGGCATTGTGAGTCCCCCTGCATCCTCCTCTTGCCCATGACTGCCATCCAGCTGATGCTGTCCCTTATGTTCCTGGATTCTTTAGCCAAGCAGCTTCCCTGATTGCAGCCCCTCTCTGCTCTCTCCTCGGTGCTGTCCTGGGGGTTAGTCACCCTGTAGAGTAGCCCCATGGCATGTGGCCTGGCAAGCATTGTGATTGATGCTGCCCGGGGGCCATGGTACACACAGGCTACGGGGAATGCCTTGTGTTTTTCTTGGGGCATGAGTAGTGATGAAAATGGCTGTAAGACTGTCAGGGTGACAGGGCTCTGGTTGGTCTCCTGTTTTCACATCGGCATCTCAGTTTCTGTGCCAAAGGGTCCACACAGACGGGTTTAGCTGCCTTCTGCTGGCCAGCTAACACTTAACCTGGCACTTCTGCTTTATCTTGCTTCATTAGTTCTTCGAACACTCATGGGCCACAAAGCCAACATCTGCAGCCTGGATTTCCACCCCTATGGTGAATTTGTGGCCTCAGGCTCCCAGGACACAAACATTAAGGTGAGGTGCCACTCAGCCACCCATCTCCCAAAATCAAGTGTAAGGGAATCTTCCCCTCGTGGCAACATCCTTACCATCCTCGGGACAGAGGACATGTGGACGCTGGCCAGACACTTCAACTTGGAATTCCCAACTTGGGCAAACCTCCAACCTTTCTCTTTGGGGCACATGACCTAGAGGTATCCAGAGTCCTCTCTGCTGAGAGCTGTACTATACCCTCTGACCTAGCCTCCAGAGACATAACAACTTCCAGTGGTTCACCCTTCCTACCCCCACCTCTGCTTCTCTCCTCTATAGCTCTGGGACATCAGGAGGAAAGGCTGTGTCTTCCGATATAGGGTAAGCATGGTCTGTGCTGGTTACCAAGCTGGTTACCACACAGAGCTTGGGTCCACACTACAGTGGGGCTGGGGGCTGCAGTCTATTGCAGGTGACTCAGAACAGACCTTGAGTAGGATGAAGGGCCTTGGGTGGTCTAGCTGGACCGCCACCCCCCCAGGCTTCCCACCAACCCCTCGAGTCACATATATTAAACTGTCCCATGCTCTGGTGTCCCCGGGTGCCGGGCTTCCTGCTCTTGTGTATGTGCAGTACTGTGGCTAACAGAATCCTTAAGGGCTTTTGGAAGAGGCCATAAAACACCTTTAACATGTAACAAACTGCATACTTGTAATTTCCTGGAGTCTTGGGAGACCACATAGAAAGTGCATACAAAGAAGTGTTCCTATGTCTCCCAGTAGTGACTACCCTCTCCGTGGCTGGACCCTAGGAAGGCCTTGTATTTTGGGGCAGGAAGTAGGGATGGGGAGGTGCTATGGGATCCAGGCCCCTCTATCGAGCTTGGCTCTTTTCCTTCTCCTCAGGGGCACAGCCAGGCTGTGCGGTGTCTCCGGTTCAGCCCTGATGGGAAGTGGCTGGCGTCGGCAGCAGATGACCACACAGTGAAGGTAACTCCTGACGTGGGCTTGCAGGCTGTGCCCAGGGGTATCCTGAGCATACACAGACGGTGTCCCTGCTGACTGTGTTTATGTGACCCTAAACTTGAGAGTAGAGAGAGGCAGCTCGGGCCCTGCCTGGCTCTTACTCTTTCCACCCTTGCCTAGCTTTGGGATCTGACGGCTGGCAAGATGATGTCCGAGTTCCCGGGTCATACAGGGCCTGTCAATGTGGTGGAGTTTCACCCCAATGAATATCTCCTAGCTTCTGGCAGCTCCGACAGGTGAGGAGGTATCCTTTCATATGTCTGAAAGCCTATGTAGACTGAATTTAAACCAGGATTGGTTCCCGGTCCCATCCCTCCCTGGCTAGTGTCCTGCTCTGTACTGACCTGTTAGGATCCACACTCCCTGGTGGGGCACACTTCAGGGGAGACCCCTGGCAATTGAGGCTAGCTCTGGGGCAGGGCTGTTGGGCACAGCCTAGGTCATCTTCACCCTCTCCTCTGCATGCCAAGGGCCATCCCTGCCCCAGACATCACTCCTAGTGCCCCTACAGGGAGGGCCCTGGAGAGGCCCTGGCTAGCATGAGGGACTGGCTTCCTGTGGCCATCTTTGCAGGACAATCCGCTTCTGGGACCTGGAGAAGTTCCAGGTGGTGAGCTGCATTGAAGGGGAGCCCGGGCCCGTCAGGTACGGGTGCTCTTCCCTGCCTCTGCTCCTACTTCTGTCCTGGGCTCACCTGCTTTCTTGGTGTCGGGTACCAGCTAGGACTGACAGCCAACTCTCCCCTGGCCTAGGAGTGTCCTCTTCAACCCCGACGGCTGCTGCTTGTACAGCGGCTGCCAGGACTCGCTGCGTGTCTACGGCTGGGAACCTGAGCGCTGCTTTGATGTGGTCCTTGTCAACTGGGGCAAGGTGGCTGACCTGGCCATCTGCAATGACCAGCTGGTGAGGGTCAAGTCACCCACCCTCCACCCTCCCCCTCCCGCTCCTACCTAAGGAGCCCCTGGGGTCCAGTCCCACTCTACACCAACTCTGTCATGTCCCGCAGATAGGTGTGGCTTTCTCCCAGAGTAATGTCTCCTCTTATGTGGTGGACCTGACGAGAGTCACCAGGACGGGAACTGTGACCCAGGACCCTGTGCAGGCTAACCAGCCTCTGACACAACAGACCCCTAACCCTGGTGTCTCCCTCCGCCGCATCTATGAGCGGCCCAGCACGACATGCAGCAAGCCTCAGAGGTGGGTGCTGATGGCCCCGGCAGGGTGTATGTCCCAAGAAGGCACCTGGATACTCAGCCCTTGGCCTCTTTGCTTGTCTCTGCACCTGCTAGGGTAAAGCACAACTCAGAGAGCGAGCGGCGCAGCCCCAGCAGCGAAGATGACAGGGACGAGCGAGAGTCCCGGGCGGAGATCCAGAACGCGGAGGACTACAATGAGATTTTCCAGCCCAAGAACAGCATCAGTGAGACCACACCCCTCCCACCTCACAGCCTGGCACCTTGCTGTGCTGTACCCCCTAAACCTTACCCGCTGTCCACATTCTTGCACCAGGCTGAATTCTAAAACCAGAAACCTAGCTGGACACCTGTAGCAGAGGCGGCACCACTTGCTGGTGGCTCCTGCCAACCTTCTGCCCAGCTCTAAGCAGGCTATAGGTGTATATGGGCTCATGGAGCCAGGAGCATGTGCTGGGGACCACTCTCTAGCCATGTGTCTATCTTAAGACCTGAGCCACATACAGGGCCTCTTCCCAGAGAGCACATGGGAACCCAAGTGTCAGCCCCCTTTGCCTTGGCAGGTCGGACACCACCCCGGAGAAGTGAGCCCTTCCCAGCACCCCCAGAAGATGGTGAGTTGGGCAGTGCTGGCCTCTAAGGACCAAGCTTTGCCTCTCCTGGCCCAAACCCTGCCCTACCCAATTAGGTCTTGTCTCTATATTTACCTAAAAGTGCCTACCATGTATCTGACCCCAGGGACGGCCCTAGAGGCAGTGTAAGCAAGGCAGAGATAGGTGCTTCTTTGAAGCCCATGCCTGAATGCTTGGGAAAAAACATGCCCCTAGGGTGTATGCTACTGAGGACTGGGGTCTACCGCAGACGTGACGTGATGGGGACTCTGAGGTTTAAGGGCTGGTGAGCAGAGGGAGAGAAAAGCTGGGGTGGGGAGAAGTTAGACAGTCATCAGAACCCAGACCTGGAGCAAGAGCCAGGGAGCCAAGTTGGAGGCCAGACACAGTGACCCCTGGCCAGAAGTTTGGGACTGAAGAAATCCAGTCGGCTTTGGGATGCCAAAGAGTCTATCCTCCAAAGCATCTGTCTCCTGTCTGCCCTTGGGAACTCAGTCCCCCCTCTTTGTCTTTATGAGACTGACAATGTTTCCTGCTCACTGCCCACTCATCTGCCCGTTTTACATCCTGCACTCCTGATGGCAGGATTGTCTGGAAACTGTTGGGTCCCATCCACACACATCCAAGGACAGCTCCTGCCTCAGCTTGCCAGGACCCTCACCTGCTGATGGGCTCCCAGCCCCCTGGTCCCCTCTGTCCATCTGCTGGCACCTGCTTCCCTTTCATGTCATGCATTCCCATCCTGCCCTCTGACCTCTTTCCACCAGGTCCATGTAGGCTGGCATTGAGCCTTTCTTTTTACTCCTAGATGCAGCCACAGTGAAGGAGGTCTCAAAACCCAGCCCAGCCATGGATGTGCAGTTGCCGCAGTTGCCGGTGCCAAATGTACGTCTGAGCAGAGAGGGCACCCTGTGGGTCTGAGCCAGGGTTGGGACATTCCTGTCAGGAGCTGAGGGATGCTCAGCTGCTGTAGGCTCAGCCTCTGCTCCCTGCAGCTCGAGGTCCCAGCACGACCGTCAGTCATGACTTCCACTCCTGCACCCAAGGGTGAACCTGACATCATCCCTGCCACACGGAATGAGCCCATTGGCTTAAAGGCCTCGGACTTCTTGCCCGTGAGTGGGACCTGCCCAAGAGGTGGGGTTGAGGGCTGAAAGGAGAATGAATGTGCGATGTGCGCCTGTGCCAGCATCTGGGTACCATCCCCACAGGCCGTGAAGGTCCCCCAGCAGGCAGAGCTGGTGGACGAGGATGCCATGTCGCAGATACGCAAAGGCCACGACACCATGTTTGTGGTGCTCACCAGCCGCCACAAGAACCTGGACACTGTCCGAGCTGTATGGACTACAGGAGATATCAAGGTGAGGGGGACTCCGAAGCCACACGGTGGGGCCCTGGGGTAGGGCAACCTTGTGTTAGAGCAGCCAGCTTCCATCAGCTGAGCACTGACGTCAGGCTGCGCCTTGCCCTCCTGTTGGCACAGTTTGTTTCATGCCTCCAACCCTGCCCACTCCCATCCCATGCTGCCCTCTTAATGGACCTTGTCTGCAGACATCAGTGGACTCTGCAGTGGCCATCAATGACCTGTCTGTGGTAGTGGACCTCCTGAACATTGTCAATCAGAAAGCGTAAGTGGCCTCGAGGAGGAGGAGAATGGGGTGGTCGGGCCGGCTGCTGGCATCATACCCAGCTGTCTCTCTAGCTCCCTGTGGAAGCTGGACCTGTGCACCACAGTGCTGCCCCAGATCGAGAAACTGCTGCAGAGCAAGTATGAGAGGTAGGGAGTCCCTCCAAACCCTGCAGCTGTCAATGGAAATAGGTGTGTGTGTGTGTGCATATGCCCATCTGTGAAAGATCCCTCTCTTTTGTCCTCTCTGACCATCTGTGTATGACTCCATCCATCTTGTTCCTGAAGCTATGTCCAGACCGGGTGCACCTCCCTGAAGCTGATCCTGCAGCGGTTCCTACCCCTCATCACTGACATTTTGGCAGCCCCGCCCTCTGTGGGTGTGGACATCAGCCGAGAGGAGAGGTAAGGGGTAAGGTGTAGTTCTTAGCCTCATTTGTGCATGGCTGTGCCCAAACAGCTTACAGAGAACAGTGTCTGAGAGTGGCTCAGGATGCTGTATGTCAGAGCACGGGCTTTTTGTGTCCCTGCACACTGCCATGGCTTGGGGGATATTTTTGTGCTCTTAGGAGGGACTGGAGTGGTTAGGGGAGCATCCTGAGATGCTACCTCTTGACCTACAGGTTGCACAAGTGTCGACTCTGCTTCAAACAACTCAAGAGCATCAGTGGCCTCGTCAAGAGCAAGTCGGGTTTGAGTGGCCGCCATGGCAGTGCCTTCCGTGAGCTGCACCTACTCATGGCCAGTTTGGACTGAGGAGCACACGGTGGGAGCACCCGCAGCCCTCACACATACATACTTGCTGTATGCCTGTGGCCCACGAGCCTCAGCCTGGCCTCCATTCCTGCCCTGCTGGCCATCCTGGAGGCATGGTTGCCAGCCTGCTGACCACCTCCTCGGTTGTGGACTAAGCTCCTCTCCGGCAGCAGCTGCAGTCTTGCACAGCCCTTACTGCTCAGGGCAACTCCAGCCTCGGGGCTGCTGCTGTAAATTATCAGGCAGATTTATTAAATTTATAACTGTTCCTGACTTCCTTATGAGAGGAATATCCGCTGGCTCTCCCAGACACATTGTGGCCTGCCTAGAGGTCGCTGGAAGCAGAGACTGTCGTACTCCAGGCCCAGTGCAGCCTCCATCTGTGGCCAGCAGGGGCCAGCAGAGTGCCAGGCACGAAGCAAGGGGCTGCCTGGTCAGCTTGACTTGGGGAAATGGAACAGGTGAGGCCATAGATGTCACATCCCCCCCAATTGTCCTTGGGTAGATTGGCTAAGAGCCTGTCATAACTCTCCCTCTGACCTCTGGATGCCACCAGAGACTAGACCACGATGGTTGCATGACCTTGGAAGATTCTAGAGTGGTTTTCACAGGCCACAGGAAACTACTTTTATACTTGTGGCATCCCTGAGAGTCTGCGCTCCAGAACAGCCTGTTGATGCAGCCAGCAAGAAGGGAGGAGCCCTAGCAAGCCCTGTTCTCCAGGGCTGGGACCGGGGACCAGATACAAACGCATACATCAGGTCACTACTAGTTACAGTTCCAGACTGGGAGGAAAGGCTGGCACAGGATGAGCAGGTCTTTGAAGCCTAGTTTTCCTTCTGTAACATGAGGTGATACTGCAATGGGATGGAATGGTTATAGGGGCCCGCAAGGTGCTAGAAATGATATAAAAAGCATGGGGGACACAGTGTCTTCAGTGACAACTTGTGGAGTCTGCATGGTCAGCCAGCAACGGGAGCAACAGGGTACACAATGCCCACTCTCCATAGGGTCTCCCAGTACTGACGATTCAGATTGCACTTGTGTAAAAAGACATTCATTTTAATAAGTAAAAATGGCTAAGCTTCCAAAAGTTCTTAAATAGGATTTGGGGAGAGGAACATGTTGAGAATCAGTGGGCAGGGAGAGTGCCTGGTGTCAGGCAGATGAACAGAACCCAGCCCTTCCACACTGGCCTCTGCCAGCCATAAATATAGCCCAGACCTCTGACAGGGTGAGGACTTCTATATACACCTAGAACATTATATACATGCCCTGTGAGGGCTCCCCTCCTCAGGAGGGTAGATAGAGATGCTCTTGCTCTCCCTAACCCCCCAGCTGGTTCCTCAAGTCTTCTCAACTAGTAGAGAACAGTCCAGCCCAAGGGCCAGGTGGGGAGAGAGCCCACCCTCCCAGCAACGGCTAGACTCCCAAGCCTACCACCAGGCAGAGTGCCATCCCTGCCCTTCCAGAAGCCCCATGCTTGGGTGCCCAGAGCCACACAGAGACTCGCTTCCTTCTGAACATGTTTCTCATCTCTGAGGAGAGGCAGGGCGGGAGAGCCTCCATCTCCTGCATTCATGGTAGGTGGGTGTTACAGCAGGTCGCCAGGCCACAGGGCCTGGCAGCGTGGCACATCTTCACACAGGCACTGGCCTCAACTTCCCTGTAGAAAAAGTAGAAAAGGAGCGCTCAGCCTCTGGCCTCAGGAGGCTTGCCCTTAGCAGATGCAGGAGGCCAGCAGAAGCACATGGACAGGGCAGCAGAAGGCAGGACTGGCTCTGTACAAGAGCCCTGGCAGAGCCCTGAACCAACAGCAGGCAGCAGGTGGGCAGCCCCAGGCCGCTGACCCCCACTTACCTGGAGACTGCAGCCCCAGCTGTCAGGCTGAAATCAAAGTGATAGATGTCTCACTTGGGGAGGGATTGGCCCAGCTGCCACCACCCCCTTTGGACCCAGTGAGGGGCCAGGCCCCCTTTTCCACCGGGGATGTCTGGACTGCTTTGTGGAAGGTGGGTAGAATCAGGTACCAGAGGAGCCCCACCAAGGTGAGGGCTCCTATATACACAGTGTGGGCCTCAAGTCAGGAGCACATGCTAGGAGCCTCTAGGGCCAGGCCCTCTGTCCCTTTCCAGGGATTTAGTAAAGCTTTCTGACTCTTGCCCAAGGCAGGCAAGGGAAGGAGACGAGTTCTAAACATGGTTTTATGGAGTTGAGGATGGAACCCAGGGCTCCCATATGCTCAGTAGGCAGCTTCCCTTTGTGGGGTACATTCTCGCTATGTAACTTCCACTAGCCTGGACCTCACTGTGTAAACCATGCTAGTTCAGAGCGTGCTCCTTCTGCCTCTCCCTCTGTCTCCCAAATGCCAGGGGCACAAGGCACAAACTATCGTGCCCAACTACCCTTTCTAGATTGTTTGGAAACTACTGATCAGGTTGGCCTCAAATCTCCACTCCTCTTGTGTCAGCCTCCCGAGCAGCTGAGATTCCAGGCTGAAGCCGCTAGGCCTGGCTCCTAGGCTGCTTCCTCCCTCTTTATAAAACAGCTTATCCTTAGCTAGGAAAGGACAGCCTGGCTCCCTCCTGCCCTCCCATCCAGTCCACTTCTAGACAGGCTTACCTGACGGCTGCAGTTTCCTTCGGATGGAACCTGGGCGGCTACTGGTCCCAGAGCCAGGGGCAGAGTGGGCTCGTGCTGTAGGCTGTGGTGTCTGGCAAGCCGGCTCCCACTGTGAGCAGAGATGCCAGTGGGTGTGGGAGCCAAGGCTGCCCCAGCTCATTCATCTCCAACCACTCTGCTTCCCTTCTCTGACAGTGTTTGTTCATCTACAGAGGGGACTAATGACCCTGCCGCCCTGAGAAGTCACGGGACAGGAATTAAACATTGTCTACAATTTAAAATGCATCTACCATGGGCAACTGCTGGTCTCCAAGACAAAGATGGCTGAAGGGGTCACGGGCATACACCCTTAATCTCAGCAGTCAGGTCTCCTGAGTTTGAGGCTAGCCTGGTCTCCAAAGCAAGCTCTAGGCCGGTCAGCGCTACATAGTGAGACCGTCTCAAGAAAGCAGAAGCACGCCTGAGCTCTGGCCTCACCCACCTTTGTGGCAGCTTCTACTCTCACTACACCAGCCTGTCTGCCCTTTCCCTACTCGGTACCAGCCTGAGTCTCACTCATGGAAGCCTCTCCCCTCCTCCCCCACCCCATCCATCCCCCAGTACCTCCAGGTGCTCCTGGCTTGACCAGGAACCCAGCTCTGTGCGGCGGGACCCAGGGCCCAACTCCACAGAGCGAACCCTCTCAGCAAATCTGAGGGAGTACAGAGTCTCGCTGGTGTTCTTCTCCACTGGGGACACCTAGGGGACAGAGAGCTCATGGTACAGCACAGGAGGTAGCAGCATGGCCACCAGAGGGTGTTTCTGGTCAGGGGAGCCCACTGTGGGCAGTGAACATGGGGAGGAACTTGTGTACTAAAAGGGGATGTTGGTAGGGGTCTATGTGGGGGTAGGGGGAGCCAGCAGCCTGGGGAGGCTCTTGCTGAACCTCAGGCCCTCACCTGTACCACCATAAGGGTCTTGCTGTCACCACTGAGTGAGTCCTGCAGCAGGTAGGTGAGTTTGGAATTGCGGAAGGGCACATGGCCTTGTCGAGAACGCAGGGCAGCAATGACGTCCCCCAGGGCGGATAGTGACCTGTTGATGTGCTGTGCCTCTCGCAGGCGGTTGCCCTCAGCCCCCGACTTGCCCACACGCTCTGAACCAGCCAGGTCCACCAGGTTCAGCTTCCCTGCAGGCAGAGCAGAGGGTGGGTCAGCGCTGCCCCAAAGATACTGATGGAGGGCTTGACCAGAATGGCCACTGTGCATCTCTCACCCGTGGTGCGGAGGCCTGTGCTGCAATCCACACCTCTCACTGTCACGATGAGCAGAGCGTGGGAGCGGGAGCTGTGTTCGTTCAGGTTGGTGAACTCGGTGGTGCGGTTGTTGTAGCCAAACTCAAACACCTGGGGGCAAAGGCAGTGAGCGGGCTCCTTTGGGATGGGCAATGCCACCCTATGTGCTTTACCTGGATACTCAAGTTTCTTCAAGGTTTTTGTTATTATTGTTTGGTTTTAGGCAGAGGCAAGGTCATAGTGTGTATACCAAGCTGCCCTCTAGCTCAGAGATCTGCCTGCCTTTGCCTCTCAAATACTGGGATAAAAGGGTGGACAGCATCACATCTTGCTATCTTTTTAATTTTTTGTTTTGAGATCAGGTCTCTGTAGTCTTGTCTGGCCTGGAACTCACTATGTAGAACTCACTGATCTGGCAGAGATCCACCTGCCTCTGTCCAAGTGCTGACATTAAAGCTGTGTACACCCAGCCACATGAAAGCCTCTGAGGGTCTTGCTAGGTAGTCAGACTGGTTCCCGATTCACAATTCTCCTGACTTAGCTTTACAGGTGTGCTCCTATACCTGCCTTTCCTTTCAGATGAGGAGACAGGCTTGGAGAGGCCTGGGGACTCCCCTAAGCTGCATTGTCAGGTGGTGGCAAAGCCCACCTTGGACCTGGAGCCCAGTTCTGTCCACCAGAGCAGCTGTTGGTGAGCGGGGCCTCCAGCACTTCCCCAGGACACCTTACCTTGTTGATGTCATCCACGCTCTGCACCTGGAACTCAGTCAGCCCTGGCACATACAGTTGCCCACTGCCATCCGGGCACAGTCGGATCTCCAGCTTCTCCTGAGGCTCTTTCCCCAGCAGGTCCCTGGAGCGGCAAGCCAAGCGAGACATGACTTCTCCCCTCCCACTCTGGCCTCTGCTTGCCTTGCACCCAATCCCTAGTCTGGAATCTGCCCCTGCACCAATCCTGGACCACAGGACTTGCACTCAGCAGAGGGGAAACAGACTTCCACAGAAGTCCTAAGGACCACACAGCCCCTGACTGGGTAGGGGCCCCATCCCGACCCAGTTCAGCGCAGCCCACAACACAGAAAGGGGTCCCAGATAAGGGAGGACCACCTAACTCCTAACCCTGGGCAGATCCTCTTCCCAGCCTCACCTAGAACTTAGACTTGGAGACCAGAAAGCCCCACTACCAGCACTCCGAGCTCCAGCCCAGGCCCACCTGAGGACTTCATTGTAAATCTCAGCTGCGCTGACGGTGATGTTGTACTGCCAGTCAGACGCCTTCTCCTGCACCTCCGAGAAGAGCAGCTGCAGGGCCCGCTGGTTGATGCCCGGGTTCTCGGGAGTCCCCTGTTGGCAAAAGTAAAGGCCCAGTGGCCCCACAGATGCTCACACAATATCTGTCACCCCATGCCCTCAGGAACCCCTAACAGGTGAGCCTCTCCTGCTGGTCTGTCTGTCCTCCGAATATAGGCAAGTTTCCCCACCTTGAAGGTAGCATTTCCCTGCCAGGAAGTTAGTAAGCATGAAAGGAGCTCTGTGGGGTGAAGCCTGACACACTACCCCTGCGGAGGGAACACCAGGGCTTATTATGGTCTGCTGGGCCTCCTGGAAGGGGCAGTTGAAATAAACATGGAGCCACCCACTGCCCACTGGGAGGCTCCCTGTCTGGGTGGCCGGAAGCCAGGCTCTGTGGGTGAAGTCCTTGAGGCTGGATGACATGGCCACCTTTCAGAATAAATCTAGAGCCCACCTGAGTGGGCCCTAGACACACTTTCCCTCCGCTTTGAAGGTACCTCCCCCTCATCTCATTCCTGTGCTTCAAGTGTGCTAACTGCCAGGCACACTCCAAGGCCTTGCAGGTCTGACTGTCCTAAGTTGTTCTACAGCAGCTCCCTCAAGGCCTCCCACGCTCCCTCAGAGGCTTCCCCCTGCCTCAGGTACCCTGTCCCTGCTGGAGCCTCACTCTGAGTTTGCTGCCGTCCCTAGCACCTGCTGGGCACCTCCTGATGTGCTGTGCTCACTGCCACTCCAACTGCCAGATGGGACCCTGACAGGCTGTTCTGTCATAGTGGGCCTCAGCAGAGAGCTCAGAGGATGGACACGACCACTATCCAGCCCAAGACAGAGCTAAATAAAGCCTTCCCCTTTCTAGCCCACCTGCCCTGCCCTGCTAACCAGAACTGAGGGTATGGGGGTTAGGGAGAGGGGTGTTAGGGGACAGTCACAACAGGGGATGCCCAGTGGCCTGACCAGCAGGAAGTGACAAGCCCCTTCCCACCCAGTCCTATGTGGGGAGTCCACTGCAGCCCCTAGTGAGTCAGAAAACTACTAACAAAGCAATACTCCATAGGGCTAAAATGTTCTATTTCTCTCTGTAGTCAGATTCTTCAACTCTGGGCTGGGAGTGTTAACATATATCTTTAATCCAAGCAGTCTGGAGGCAGAGGCAGGTGGATCTCTGAGTTCAAGGTCAGCCTGACTAACAGCGAGTTCTAGGACAGCCAGGGCTACATTGGAGACCATGTCTCAAAACAATAGCAAAACAAAATTCTTCTGCTCAAGTAGCCTTACAAGGCTGGCTCTGGGCACACACCCTGGATAAAGCCCTTCTCTGAGAGCCCAGCTTCCCACAACTGTAGTCGTGCAGGTATCCAGTCTCTCCCATGGCC
GF3 -1 -1 1 GAAGAGCGGAGTGTTGCGAGGAAG
GF4 -1 -1 1 GCTGAAGAGCGGAGTGTTGCGAGGAAG
EX52 -1 -1 1 GCTAAAAGTAGGCCTTCCTCCTTC
EX32 -1 -1 1 GAAGGAGGAAGGCCTACTTTTAGCAG
GR3 -1 -1 1 GCCGTAGACACGCAGCGAGTCCTG
GR4 -1 -1 1 CTTCACTGTGTGGTCATCTGCTGCCGACG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000031787 -- 97613860 -- 97613977 ENSMUSE00000212976 -- ENSMUSE00000212976
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:8:1:131738871:1, start 97613859: end 97613977
Found 9 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:8:97607360:97608359_163 G3:8:97617477:97618476_684
ACCEPT (0) gap pair G5:8:97607360:97608359_163 G3:8:97617477:97618476_657
ACCEPT (0) gap pair G5:8:97607360:97608359_163 G3:8:97617477:97618476_664
ACCEPT (0) gap pair G5:8:97607360:97608359_578 G3:8:97617477:97618476_684
ACCEPT (0) gap pair G5:8:97607360:97608359_578 G3:8:97617477:97618476_657
ACCEPT (0) gap pair G5:8:97607360:97608359_578 G3:8:97617477:97618476_664
ACCEPT (0) gap pair G5:8:97607360:97608359_216 G3:8:97617477:97618476_684
REJECT (1) gap pair G5:8:97607360:97608359_216 G3:8:97617477:97618476_657
REJECT (1) gap pair G5:8:97607360:97608359_216 G3:8:97617477:97618476_664
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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