Predicted Targets of mmu-miR-25 Based on Experimental Evidence
Illumina Probe IDs | Gene Symbols | Gene Name | Entrez ID | Log Fold Change upon Dgcr8 Depletion | P-value of change upon Dgcr8 Depletion | Log Fold Change upon miR-25 Reintroduction | P-value of change upon miR-25 Reintroduction | GO Terms | KEGG Pathways | Transcript used for 3'UTR seed counts | 6mer(1A)-GCAATA | 6mer(3)-GTGCAA | 6mer(2)-TGCAAT | 7mer(2)(m8)-GTGCAAT | 7mer(1A)-TGCAATA | 8mer(1A)-GTGCAATA | Target Scan Conserved Target Context Score | Target Scan Non-conserved Target Context Score | miRBase Targets P-value
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iXuHlOb1FICWdz.rd4 | Tmem184b | transmembrane protein 184b | 223693 | 1.23 | 1.0e-18 | 0.43 | 6.4e-05 |  |  | ENSMUST00000074991 | 1 | 1 | 1 | 1 | 1 | 1 | -0.1188 | Not Found | Not Found
Bp5e4GiPe018Rk3.L4 | Adam23 | a disintegrin and metallopeptidase domain 23 | 23792 | 1.26 | 4.2e-16 | 0.71 | 1.2e-05 | cell adhesion(GO:0007155) | proteolysis(GO:0006508) | integrin-mediated signaling pathway(GO:0007229) |  | OTTMUST00000004901 | 2 | 2 | 5 | 2 | 2 | 1 | -0.3949 | Not Found | Not Found
lkuSKIB557us.i4.5I | Plekhm1 | pleckstrin homology domain containing, family M (with RUN domain) member 1 | 353047 | 0.94 | 5.5e-16 | 0.98 | 3.2e-05 | intracellular signaling cascade(GO:0007242) |  | OTTMUST00000005762 | 2 | 2 | 2 | 1 | 2 | 1 | -0.1376 | -0.3862 | Not Found
Ej5ewT_J98eO7nrqEo | Whsc1l1 | Wolf-Hirschhorn syndrome candidate 1-like 1 (human) | 234135 | 0.66 | 3.9e-15 | 0.56 | 2.0e-05 | transcription(GO:0006350) | regulation of transcription, DNA-dependent(GO:0006355) | chromatin modification(GO:0016568) |  | ENSMUST00000084026 | 1 | 3 | 3 | 1 | 1 | 0 | Not Found | Not Found | Not Found
0Xul8aOr_34e.kHjnk uLp8e6gnXujLOheU7o | Rab8b | RAB8B, member RAS oncogene family | 235442 | 0.61 | 4.7e-15 | 0.89 | 7.3e-08 | transport(GO:0006810) | small GTPase mediated signal transduction(GO:0007264) | protein transport(GO:0015031) |  | ENSMUST00000041139 | 1 | 2 | 1 | 1 | 1 | 1 | Not Found | -0.342 | Not Found
oKJsk.kySo_5ezce7U | Gcnt2 | glucosaminyl (N-acetyl) transferase 2, I-branching enzyme | 14538 | 0.76 | 1.1e-13 | 0.45 | 1.2e-04 |  | Glycosphingolipid biosynthesis - neo-lactoseries(00602) | Glycan structures - biosynthesis 2(01031) | ENSMUST00000110191 | 1 | 0 | 2 | 0 | 1 | 0 | Not Found | -0.046 | Not Found
3uKIKTKCuIcniH3tOk | Bicd2 | bicaudal D homolog 2 (Drosophila) | 76895 | 0.63 | 9.8e-12 | 0.39 | 1.6e-04 | microtubule-based movement(GO:0007018) |  | ENSMUST00000048544 | 0 | 1 | 2 | 0 | 0 | 0 | Not Found | Not Found | Not Found
EeMSH_Qure_2ep4910 | 4931407G18Rik | RIKEN cDNA 4931407G18 gene | 70977 | 0.75 | 9.2e-11 | 0.57 | 3.3e-05 |  |  | OTTMUST00000052375 | 0 | 0 | 0 | 0 | 0 | 0 | Not Found | Not Found | Not Found
um2NuXFnc0Od13BQqg |  |  |  | 0.55 | 1.2e-10 | 0.77 | 6.5e-06 |  |  |  |  |  |  |  |  |  |  |  |
ldWImLHEDmUk16IL30 | Pcaf * |  | 18519* | 0.55 | 1.5e-10 | 0.48 | 1.8e-04 |  |  | ENSMUST00000000724 | 2 | 1 | 1 | 1 | 1 | 1 | -0.5192 | Not Found | Not Found
cuzncl0lPHj0yUv0Xw | Ccnc | cyclin C | 51813 | 0.40 | 1.6e-10 | 0.93 | 1.2e-09 | transcription(GO:0006350) | regulation of transcription, DNA-dependent(GO:0006355) |  | OTTMUST00000010608 | 1 | 2 | 1 | 1 | 1 | 1 | -0.4342 | Not Found | Not Found
c0rHdgr7fnpXuuvlDo | Plekhg3 | pleckstrin homology domain containing, family G (with RhoGef domain) member 3 | 263406 | 0.62 | 1.0e-09 | 0.48 | 1.1e-04 | intracellular signaling cascade(GO:0007242) | regulation of Rho protein signal transduction(GO:0035023) |  | ENSMUST00000101279 | 1 | 0 | 0 | 0 | 0 | 0 | Not Found | Not Found | Not Found
Hg.3.j_oj70SgstdV0 | Adam19 * |  | 11492* | 0.65 | 1.6e-09 | 0.58 | 1.2e-04 |  |  | OTTMUST00000012216 | 3 | 1 | 3 | 1 | 3 | 1 | -0.3185 | -0.0716 | Not Found
WfXy3tUW5N58XTpnv4 | Rnf38 | ring finger protein 38 | 73469 | 0.62 | 2.2e-09 | 0.94 | 3.2e-05 |  |  | OTTMUST00000015974 | 2 | 3 | 3 | 2 | 1 | 1 | -0.3052 | Not Found | Not Found
0Xgh4_noK_vkoCFolU | Acbd4 | acyl-Coenzyme A binding domain containing 4 | 67131 | 0.43 | 3.9e-09 | 0.46 | 6.7e-05 | biological_process(GO:0008150) |  | OTTMUST00000006883 | 0 | 1 | 1 | 1 | 0 | 0 | Not Found | -0.2064 | 0.0318833
KrnNSu8OCCeiikq5ag | 4930519N13Rik | RIKEN cDNA 4930519N13 gene | 78177 | 0.45 | 4.8e-09 | 0.40 | 1.2e-04 |  |  | OTTMUST00000037530 | 0 | 1 | 1 | 1 | 0 | 0 | Not Found | -0.2048 | 0.00937581
ZZw4u1Ikd1fin5rUKI | Leprot | leptin receptor overlapping transcript | 230514 | 0.47 | 4.9e-09 | 0.87 | 9.3e-07 |  |  | OTTMUST00000019180 | 0 | 2 | 1 | 1 | 0 | 0 | Not Found | Not Found | 0.0246051
ohJKUK40unAvgCq_v0 | Ric8b | resistance to inhibitors of cholinesterase 8 homolog B (C. elegans) | 237422 | 0.47 | 6.7e-09 | 0.70 | 1.7e-05 |  |  | ENSMUST00000038523 | 0 | 1 | 1 | 1 | 0 | 0 | Not Found | -0.1647 | Not Found
W3K5fkivgpSUZR59RQ |  |  |  | 0.38 | 7.4e-09 | 0.51 | 1.2e-05 |  |  |  |  |  |  |  |  |  |  |  |
E8S57gidW_K_urHtf4 imb8_k2n0aNqiOX2H8 | Necap1 | NECAP endocytosis associated 1 | 67602 | 0.67 | 1.1e-08 | 1.27 | 6.4e-07 | transport(GO:0006810) | endocytosis(GO:0006897) | protein transport(GO:0015031) |  | ENSMUST00000032477 | 1 | 1 | 1 | 1 | 1 | 1 | -0.4297 | Not Found | Not Found
9nJyRQvr4SPv_45_V4 | Wwp2 | WW domain containing E3 ubiquitin protein ligase 2 | 66894 | 0.31 | 1.5e-08 | 0.62 | 2.0e-04 | protein modification process(GO:0006464) | Ubiquitin mediated proteolysis(04120) | Dentatorubropallidoluysian atrophy (DRPLA)(05050) |  |  |  |  |  |  |  | -0.4071 | Not Found | Not Found
6B6Xp7uTy0O1T6.53s | D16Ertd472e | DNA segment, Chr 16, ERATO Doi 472, expressed | 67102 | 0.46 | 8.0e-08 | 0.70 | 4.5e-06 |  |  | ENSMUST00000023571 | 2 | 1 | 0 | 0 | 0 | 0 | Not Found | Not Found | Not Found
oronqodncvXoVAxvxE | Lmbr1l | limb region 1 like | 74775 | 0.29 | 2.7e-07 | 0.75 | 2.3e-06 | endocytosis(GO:0006897) |  | OTTMUST00000058993 | 2 | 1 | 1 | 1 | 1 | 1 | -0.3165 | Not Found | 0.00104278
lW8eG7B8kk8i4bfS_s | Snap29 | synaptosomal-associated protein 29 | 67474 | 0.49 | 2.9e-07 | 0.66 | 1.1e-04 | transport(GO:0006810) | protein transport(GO:0015031) | SNARE interactions in vesicular transport(04130) | OTTMUST00000057883 | 1 | 1 | 1 | 1 | 0 | 0 | Not Found | Not Found | Not Found
HQhFkIdI9XSOFUmVf4 cSdHs4EaE_eHrDgud0 | Gnaq | guanine nucleotide binding protein, alpha q polypeptide | 14682 | 0.33 | 3.2e-07 | 0.98 | 2.6e-06 | skeletal development(GO:0001501) | regulation of action potential(GO:0001508) | signal transduction(GO:0007165) | protein amino acid ADP-ribosylation(GO:0006471) | G-protein coupled receptor protein signaling pathway(GO:0007186) | G-protein signaling, adenylate cyclase activating pathway(GO:0007189) | glutamate signaling pathway(GO:0007215) | heart development(GO:0007507) | behavior(GO:0007610) | post-embryonic development(GO:0009791) | neuron remodeling(GO:0016322) | forebrain neuron development(GO:0021884) | embryonic digit morphogenesis(GO:0042733) | regulation of melanocyte differentiation(GO:0045634) | pigmentation during development(GO:0048066) | dopamine receptor, phospholipase C activating pathway(GO:0060158) | Calcium signaling pathway(04020) | Gap junction(04540) | Long-term potentiation(04720) | Long-term depression(04730) | GnRH signaling pathway(04912) | Melanogenesis(04916) | ENSMUST00000025541 | 0 | 2 | 2 | 2 | 0 | 0 | Not Found | Not Found | Not Found
0uTv0tSkLxPa_7kMgo | Osbpl2 | oxysterol binding protein-like 2 | 228983 | 0.37 | 4.3e-07 | 0.64 | 4.3e-06 | transport(GO:0006810) | lipid transport(GO:0006869) | steroid metabolic process(GO:0008202) |  | OTTMUST00000038983 | 1 | 1 | 1 | 1 | 1 | 1 | Not Found | -0.2365 | 0.0127902
Bh9F3khPV5KzHUS_UA | Brms1l * |  | 52592* | 0.35 | 9.5e-07 | 0.58 | 4.6e-05 |  |  | ENSMUST00000012220 | 0 | 1 | 1 | 1 | 0 | 0 | -0.1796 | Not Found | Not Found
lenfuu6HT5eEyFcn1Q | Gli2 | GLI-Kruppel family member GLI2 | 14633 | 0.68 | 1.5e-06 | 0.47 | 6.3e-05 | negative regulation of transcription from RNA polymerase II promoter(GO:0000122) | skeletal development(GO:0001501) | kidney development(GO:0001822) | osteoblast development(GO:0002076) | pattern specification process(GO:0007389) | axon guidance(GO:0007411) | ventral midline development(GO:0007418) | hindgut morphogenesis(GO:0007442) | heart development(GO:0007507) | positive regulation of cell proliferation(GO:0008284) | dorsal/ventral pattern formation(GO:0009953) | proximal/distal pattern formation(GO:0009954) | floor plate formation(GO:0021508) | spinal cord dorsal/ventral patterning(GO:0021513) | ventral spinal cord development(GO:0021517) | cerebellar cortex morphogenesis(GO:0021696) | smoothened signaling pathway involved in ventral spinal cord interneuron specification(GO:0021775) | smoothened signaling pathway involved in spinal cord motor neuron cell fate specification(GO:0021776) | dorsoventral neural tube patterning(GO:0021904) | smoothened signaling pathway in regulation of granule cell precursor cell proliferation(GO:0021938) | spinal cord ventral commissure morphogenesis(GO:0021965) | pituitary gland development(GO:0021983) | cell differentiation(GO:0030154) | lung development(GO:0030324) | mammary gland development(GO:0030879) | hindbrain development(GO:0030902) | tube development(GO:0035295) | odontogenesis of dentine-containing tooth(GO:0042475) | positive regulation of neuron differentiation(GO:0045666) | positive regulation of transcription(GO:0045941) | positive regulation of transcription from RNA polymerase II promoter(GO:0045944) | embryonic gut development(GO:0048566) | developmental growth(GO:0048589) | anatomical structure formation(GO:0048646) | neuron development(GO:0048666) | branching morphogenesis of a tube(GO:0048754) | anatomical structure development(GO:0048856) | notochord regression(GO:0060032) | Hedgehog signaling pathway(04340) | Basal cell carcinoma(05217) | ENSMUST00000062483 | 0 | 0 | 0 | 0 | 0 | 0 | Not Found | Not Found | Not Found
fuf5P1KK.Vzv6sA78k | Znrf2 | zinc and ring finger 2 | 387524 | 0.29 | 3.3e-06 | 0.90 | 6.2e-05 | biological_process(GO:0008150) |  | OTTMUST00000056945 | 1 | 1 | 1 | 1 | 1 | 1 | Not Found | -0.2721 | Not Found
HLkRZF_ztElS698r3s | Ttc39b | tetratricopeptide repeat domain 39B | 69863 | 0.51 | 6.4e-06 | 0.68 | 1.8e-04 |  |  | OTTMUST00000000091 | 2 | 3 | 3 | 1 | 2 | 0 | Not Found | Not Found | Not Found
TbIXcNVESkpfvK1MuI | Dbt | dihydrolipoamide branched chain transacylase E2 | 13171 | 0.24 | 2.2e-05 | 0.75 | 2.4e-06 | metabolic process(GO:0008152) | Valine, leucine and isoleucine degradation(00280) | OTTMUST00000066974 | 0 | 1 | 1 | 1 | 0 | 0 | Not Found | -0.2365 | 0.000120217
ECyrniJ06TKt568y6I | Dcbld1 | discoidin, CUB and LCCL domain containing 1 | 66686 | 0.45 | 5.2e-05 | 1.04 | 4.5e-05 |  |  |  |  |  |  |  |  |  | Not Found | Not Found | 0.00284702
xQMgji9qDrurjHh1Kk | Sfxn3 | sideroflexin 3 | 94280 | 0.49 | 6.6e-05 | 0.45 | 7.6e-05 | transport(GO:0006810) | ion transport(GO:0006811) | cation transport(GO:0006812) | iron ion transport(GO:0006826) |  | ENSMUST00000062213 | 0 | 0 | 2 | 0 | 0 | 0 | Not Found | Not Found | Not Found
fi_CR.P_vu.jRvefoI | Rgma | RGM domain family, member A | 244058 | 0.26 | 7.3e-05 | 0.41 | 3.4e-05 | neural tube closure(GO:0001843) | positive regulation of protein amino acid phosphorylation(GO:0001934) | regulation of BMP signaling pathway(GO:0030510) |  | OTTMUST00000077776 | 0 | 0 | 0 | 0 | 0 | 0 | Not Found | Not Found | Not Found
Eeuq_mu5hHURiKdEM8 | Ndufa5 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 | 68202 | 0.24 | 8.0e-05 | 1.40 | 7.4e-08 | respiratory electron transport chain(GO:0022904) | transport(GO:0006810) | Oxidative phosphorylation(00190) | OTTMUST00000066417 | 0 | 1 | 1 | 1 | 0 | 0 | Not Found | -0.3131 | 0.00396649
6X9TfVN8x7_f_OKfqo |  |  |  | 0.42 | 1.1e-04 | 0.50 | 5.6e-06 |  |  |  |  |  |  |  |  |  |  |  |
ry1e1qOt947dcQ5riQ | Ppm1b * |  | 19043* | 0.22 | 4.3e-04 | 0.60 | 1.4e-04 |  |  | ENSMUST00000112304 | 1 | 0 | 0 | 0 | 0 | 0 | Not Found | Not Found | Not Found
oUuo767U67hPAhSgl4 | Hus1 | Hus1 homolog (S. pombe) | 15574 | 0.22 | 4.6e-04 | 0.87 | 6.2e-07 | DNA damage checkpoint(GO:0000077) | double-strand break repair via homologous recombination(GO:0000724) | DNA repair(GO:0006281) | protein amino acid phosphorylation(GO:0006468) | response to DNA damage stimulus(GO:0006974) | embryonic development(GO:0009790) | negative regulation of DNA replication(GO:0008156) | response to UV(GO:0009411) | G1/S transition checkpoint(GO:0031575) |  | OTTMUST00000011503 | 1 | 0 | 2 | 0 | 1 | 0 | Not Found | Not Found | Not Found
ld.9cvPeXlLBVKCLck | Asah1 | N-acylsphingosine amidohydrolase 1 | 11886 | 0.22 | 5.7e-04 | 0.60 | 1.4e-05 |  | Sphingolipid metabolism(00600) | OTTMUST00000054076 | 1 | 1 | 1 | 1 | 0 | 0 | Not Found | -0.1342 | 0.0236953
3zeTwolFuqW9i4Vkjs | Pols | polymerase (DNA directed) sigma | 210106 | 0.22 | 1.4e-03 | 0.60 | 4.5e-05 | DNA replication(GO:0006260) | cell cycle(GO:0007049) | mitosis(GO:0007067) | cell division(GO:0051301) |  | OTTMUST00000081019 | 2 | 1 | 1 | 1 | 1 | 1 | -0.4701 | Not Found | Not Found
9EKV3r68p3OAfM.lf0 |  |  |  | 0.17 | 1.7e-03 | 0.75 | 8.5e-05 |  |  |  |  |  |  |  |  |  |  |  |
re7f7od_kU_3ejq6mA | Prkar1a | protein kinase, cAMP dependent regulatory, type I, alpha | 19084 | 0.16 | 2.9e-03 | 0.99 | 7.6e-09 | mesoderm formation(GO:0001707) | regulation of protein amino acid phosphorylation(GO:0001932) | signal transduction(GO:0007165) | protein amino acid phosphorylation(GO:0006468) | organ morphogenesis(GO:0009887) | cell proliferation(GO:0008283) | Apoptosis(04210) | Insulin signaling pathway(04910) | OTTMUST00000007044 | 0 | 2 | 1 | 1 | 0 | 0 | Not Found | -0.2854 | Not Found
Kl70hUQdU3V_lEg7Hw | Spryd4 | SPRY domain containing 4 | 66701 | 0.17 | 5.0e-03 | 1.25 | 7.7e-10 |  |  | ENSMUST00000061995 | 1 | 1 | 1 | 1 | 1 | 1 | -0.4078 | Not Found | Not Found
TJDtEB5LLt2heVnRSc | Fbxw7 * |  | 50754* | 0.28 | 6.4e-03 | 0.75 | 2.6e-06 |  |  | OTTMUST00000054321 | 1 | 3 | 2 | 2 | 1 | 1 | -0.5395 | Not Found | Not Found