Auditory Brainstem Response (ABR) measurement for Calcoco2tm1(KOMP)WtsiMGP Select

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Calcoco2<tm1(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MDHD25.3e M00993311 Calcoco2/Calcoco2(locked) Female 13.3 25 30 10 15 35 40
MDHD25.3f M00993312 Calcoco2/Calcoco2(locked) Female 13.3 25 25 25 20 30 30
MDHD27.1c M00993287 Calcoco2/Calcoco2(locked) Female 14.1 25 35 15 20 25 30
MDHD32.2b M01057359 Calcoco2/Calcoco2(locked) Male 14.9 25 30 20 25 30 35
Calcoco2/Calcoco2 Average 13.9 25 30 17.5 20 30 33.75
Calcoco2/Calcoco2 Standard Deviation 0.66 0 3.54 5.59 3.54 3.54 4.15
Calcoco2/Calcoco2 Standard Error 0.33 0 1.77 2.8 1.77 1.77 2.07
MDHD27.1d M00993288 Calcoco2:failed(locked) Female 14.1 25 30 20 15 20 25
Calcoco2:failed Average 14.1 25 30 20 15 20 25
Calcoco2:failed Standard Deviation 0.0 0 0 0 0 0 0
Calcoco2:failed Standard Error 0.0 0 0 0 0 0 0
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAYV79.1f M00986536 +/+(locked) Female 14.2 20 25 25 20 35 45
MAYV79.1g M00986537 +/+(locked) Female 14.2 30 35 15 20 25 45
MBUX66.1e M01082998 +/+(locked) Female 14.1 30 35 20 30 30 50
MBUX66.1j M01083003 +/+(locked) Female 14.1 30 40 25 30 40 65
MDJJ52.1c M01164692 +/+(locked) Female 14.1 25 30 15 25 20 25
MEEB13.2g M01167983 +/+(locked) Female 14.3 25 30 15 15 25 35
MEEB13.2j M01167986 +/+(locked) Female 14.3 25 30 25 20 25 35
MCKW56.3a M01106664 +/+(locked) Male 13.8 25 30 25 20 40 45
MCKW56.3b M01106665 +/+(locked) Male 13.8 25 30 15 15 40 50
MDAL49.1a M01056684 +/+(locked) Male 14.2 25 40 25 20 30 35
MDAL49.1b M01056685 +/+(locked) Male 14.2 25 35 20 25 35 80
MDAL49.1d M01056687 +/+(locked) Male 14.2 20 30 20 20 25 30
+/+ Average 14.13 25.42 32.5 20.42 21.67 30.83 45
+/+ Standard Deviation 0.16 3.2 4.33 4.31 4.71 6.72 14.72
+/+ Standard Error 0.05 .92 1.25 1.24 1.36 1.94 4.25
MDAL47.1b M01056674 Tmod1:failed(locked) Male 14.2 30 35 25 25 35 40
Tmod1:failed Average 14.2 30 35 25 25 35 40
Tmod1:failed Standard Deviation 0.0 0 0 0 0 0 0
Tmod1:failed Standard Error 0.0 0 0 0 0 0 0

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL