Phenotyping Data for Adam17 (MBAD) Associated with 'behavior/neurological' (MP:0005386)

Open Field: Open Field (10 minutes)
Open Field (week 9) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Anxiety and exploratory drive are assessed over 10 minutes using open field apparatus (PhenoMaster ActiMot; TSE Systems)
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Total Distance Travelled Whole Arena
Whole Arena Resting Time
Whole Arena Average Speed
Total Rears
Total Distance Travelled - Periphery
Total Rest Time at Periphery
Total Time at Periphery
Average Speed at Periphery
Total Distance Travelled - Centre
Total Resting Time in Centre
Total Time in Centre
Average Speed at Centre
Latency To Enter Centre
Number Entries to Centre
% Distance Moved in Centre - Total
% Time Spent in Centre - Total
Grip Strength: Standard Protocol
Grip Strength (week 9) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Neuromuscular function and muscle strength are assessed with a Bioseb grip strength meter. Three trials each of fore paws and all paws are performed immediately following each other.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Grip Strength Fore Paws Trial 1
Grip Strength Fore Paws Trial 2
Grip Strength Fore Paws Trial 3
Grip Strength All Paws Trial 1
Grip Strength All Paws Trial 2
Grip Strength All Paws Trial 3
Eye Morphology: Eye Morphology v2
Eye Morphology (week 15) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are assessed for gross morphological changes to the eye using a slit lamp (Zeiss SL130) and ophthalmoscope (Heine Omega 500). The eye is examined both undilated and dilated (tropicamide and/or neosynephrine). Images on the slit lamp are collected using a Leica DFC420. Images of the fundus are collected by using a topical endoscope (BERCI Tele-Otoscope with HOPKINS straight forward 0?, diameter 3mm, Halogen cold light fountain light source) and camera (Nikon D40x with Nikon AF 85mm F1.8D AF Nikkor lens).
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Pupil Light Response
Stress Induced Hyperthermia: Standard Protocol
Core Temperature and Stress Induced Hyperthermia (15 weeks) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Basal core body temperature is measured rectally using a TH-5 thermometer with a RET-3 probe (Viking Medical). The mice are thereafter placed in a clean cage and a second reading is taken after 15-30 minutes.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Post Stress Temperature
Change in Temperature
Indirect Calorimetry: Standard Protocol
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Total VO2, VCO2, activity and energy expenditure refers to an average during the total time period. Water bottles are weighed before and after the experiment to assess water consumption.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Male
Het
population
parameter
Graph MP Annotation
Cumulative Food Intake
Water Intake
X-Activity Total
X-Activity Ambulatory
Hot Plate: Standard Protocol
Hotplate (week 10) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are assessed for thermal pain perception using a hotplate (TSE systems). Each mouse is placed on the plate heated to 52?C, and the latency to the first response, and response type, are recorded. Mice that do not respond after 30 seconds are removed.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Male
Het
population
parameter
Graph MP Annotation
Latency to respond