Auditory Brainstem Response (ABR) measurement for Cadm1tm1.2BrdMGP Pipeline 2

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Cadm1<tm1.2Brd> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMQ111.1d M00047154 TSL/+(locked) Female 13.1 34 55 31 31 43 43
MAMQ112.1g M00047161 TSL/+(locked) Female 12.8 43 34 13 22 31 31
MAMQ118.1a M00048218 TSL/+(locked) Male 12.6 22 43 13 31 37 37
TSL/+ Average 12.83 33 44 19 28 37 37
TSL/+ Standard Deviation 0.21 8.6 8.6 8.49 4.24 4.9 4.9
TSL/+ Standard Error 0.12 4.97 4.97 4.9 2.45 2.83 2.83
MAMQ112.1e M00047159 TSL/TSL(locked) Female 12.8 31 52 37 31 58 55
MAMQ115.1d M00045760 TSL/TSL(locked) Female 13.1 34 37 31 31 40 61
MAMQ115.1f M00045762 TSL/TSL(locked) Female 13.1 34 40 34 46 43 37
MAMQ118.1f M00048223 TSL/TSL(locked) Female 12.6 34 58 40 31 49 49
MAMQ123.1f M00045796 TSL/TSL(locked) Female 12.8 31 46 10 34 46 40
MAMQ118.1b M00048219 TSL/TSL(locked) Male 12.6 34 46 25 28 52 55
MAMQ118.1c M00048220 TSL/TSL(locked) Male 12.6 43 37 13 25 43 49
MAMQ120.1c M00048243 TSL/TSL(locked) Male 12.9 28 37 10 28 25 34
MAMQ120.1d M00048244 TSL/TSL(locked) Male 12.9 43 46 28 40 49 70
MAMQ121.1c M00045783 TSL/TSL(locked) Male 13.2 34 52 22 46 43 43
TSL/TSL Average 12.86 34.6 45.1 25 34 44.8 49.3
TSL/TSL Standard Deviation 0.21 4.61 6.98 10.48 7.1 8.29 10.69
TSL/TSL Standard Error 0.07 1.46 2.21 3.31 2.24 2.62 3.38
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMQ111.1c M00047153 +/+(locked) Female 13.1 37 61 34 43 46 49
MAMQ123.1h M00045798 +/+(locked) Female 12.8 37 43 16 25 31 37
MAMQ98.1h M00045696 +/+(locked) Female 13.1 34 43 25 28 37 40
MAMQ117.1e M00048215 +/+(locked) Male 13.0 31 40 13 34 37 37
MAMQ117.1f M00048216 +/+(locked) Male 13.0 37 40 13 31 34 40
MAMQ121.1a M00045781 +/+(locked) Male 13.2 22 52 13 25 31 31
MAMQ121.1b M00045782 +/+(locked) Male 13.2 31 43 19 31 37 37
+/+ Average 13.06 32.71 46 19 31 36.14 38.71
+/+ Standard Deviation 0.13 5.03 7.17 7.35 5.78 4.73 5.03
+/+ Standard Error 0.05 1.9 2.71 2.78 2.19 1.79 1.9