Auditory Brainstem Response (ABR) measurement for Arid4atm1a(EUCOMM)WtsiMouse GP

Results Summary

  • ABR thresholds normal
  • Click ABR waveform shape abnormal
  • Click ABR wave 3 amplitude abnormal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Arid4a<tm1a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAAJ47.1c M00331930 Arid4a/+(locked) Female 13.9 25 35 25 25 30 60
Arid4a/+ Average 13.9 25 35 25 25 30 60
Arid4a/+ Standard Deviation 0.0 0 0 0 0 0 0
Arid4a/+ Standard Error 0.0 0 0 0 0 0 0
MAAJ47.1d M00331931 Arid4a/Arid4a(locked) Female 13.9 30 35 20 25 40 65
MAAJ47.1e M00331932 Arid4a/Arid4a(locked) Female 13.9 25 40 15 10 25 35
MAAJ129.2b M00523099 Arid4a/Arid4a(locked) Male 13.9 25 35 15 20 20 35
MAAJ27.1a M00218552 Arid4a/Arid4a(locked) Male 14.1 20 45 20 20 25 40
MAAJ30.1a M00218577 Arid4a/Arid4a(locked) Male 13.9 30 60 30 30 35 55
Arid4a/Arid4a Average 13.94 26 43 20 21 29 46
Arid4a/Arid4a Standard Deviation 0.08 3.74 9.27 5.48 6.63 7.35 12
Arid4a/Arid4a Standard Error 0.04 1.67 4.15 2.45 2.97 3.29 5.37
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBDK30.2e M00416608 +/+(locked) Female 13.9 20 30 15 20 25 40
MBDK30.2f M00416609 +/+(locked) Female 13.9 20 35 20 25 25 35
MBDK31.3e M00416614 +/+(locked) Female 13.6 20 30 10 15 25 30
MBEQ54.1f M00425103 +/+(locked) Female 14.1 20 30 10 20 25 35
MBEQ54.1h M00425105 +/+(locked) Female 14.1 20 40 20 10 30 30
MBKW35.7c M00326323 +/+(locked) Female 14.4 25 45 15 15 25 40
MBKW35.7d M00326324 +/+(locked) Female 14.4 25 45 20 25 25 30
MBLU60.2g M00362818 +/+(locked) Female 13.9 25 30 10 20 30 30
MBLU60.2h M00362819 +/+(locked) Female 13.9 25 35 15 30 30 35
MBTT56.1g M00454912 +/+(locked) Female 13.9 25 35 15 30 35 55
MBTT56.1h M00457321 +/+(locked) Female 13.9 25 30 10 20 30 40
MAFN52.3b M00350158 +/+(locked) Male 13.9 20 35 15 20 25 30
MAFN52.3c M00350159 +/+(locked) Male 13.9 15 30 10 20 20 25
MAKT43.1b M00327966 +/+(locked) Male 13.9 25 60 20 20 20 40
MAKT48.1a M00327971 +/+(locked) Male 14.5 25 30 20 20 20 30
MBDH44.2a M00533133 +/+(locked) Male 13.9 25 30 15 15 25 40
MBFF151.1b M00453226 +/+(locked) Male 13.9 20 30 15 20 25 35
MBFF151.1c M00453227 +/+(locked) Male 13.9 20 25 5 20 30 35
MBKW27.1a M00219976 +/+(locked) Male 14.2 30 45 20 30 30 55
MBKW28.1b M00219985 +/+(locked) Male 14.2 20 40 15 15 25 35
MBKW28.1c M00219986 +/+(locked) Male 14.2 25 40 15 20 25 25
MBKW34.1d M00220028 +/+(locked) Male 14.2 20 35 15 20 25 35
MBLD14.2c M00382700 +/+(locked) Male 13.5 25 30 10 20 25 40
MBLD18.2b M00382682 +/+(locked) Male 13.9 20 35 15 20 25 35
+/+ Average 14.0 22.5 35.42 14.58 20.42 26.04 35.83
+/+ Standard Deviation 0.23 3.23 7.49 4.06 4.77 3.53 7.31
+/+ Standard Error 0.05 .66 1.53 .83 .97 .72 1.49

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL

Input Output Functions