Auditory Brainstem Response (ABR) measurement for Akt2tm1e(KOMP)WtsiMGP Pipeline 2

Results Summary

  • ABR thresholds normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean Threshold is plotted (+/- population standard deviation). The green shaded area indicates the mean threshold (+/- 1SD and +/- 2SD) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and are plotted as a function of absolute sound pressure level (SPL) and SPL relative to threshold. Means are plotted (+/- population standard deviation).

ABR Thresholds Summary

ABR Thresholds for Akt2<tm1e(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
MAAE77.1c M00019652 +/Akt2(locked) Female 12.8 46 25 19 31 49
MAAE20.1a M00012669 +/Akt2(locked) Male 13.2 49 16 25 37 46
MAAE20.1b M00012670 +/Akt2(locked) Male 13.2 46 28 31 46 46
MAAE42.1a M00012734 +/Akt2(locked) Male 13.3 52 16 22 37 49
MAAE42.1c M00012736 +/Akt2(locked) Male 13.3 52 28 28 37 49
MAAE42.1d M00012737 +/Akt2(locked) Male 13.3 43 22 25 37 46
MAAE63.1b M00019596 +/Akt2(locked) Male 12.4 52 22 25 37 55
+/Akt2 Average 13.07 48.57 22.43 25 37.43 48.57
+/Akt2 Standard Deviation 0.32 3.37 4.66 3.59 4.07 2.97
+/Akt2 Standard Error 0.12 1.28 1.76 1.36 1.54 1.12
MAAE63.1c M00019597 Akt2/Akt2(locked) Female 12.5 49 22 22 43 61
MAAE63.1f M00019600 Akt2/Akt2(locked) Female 12.5 46 19 22 37 58
MAAE65.1f M00019611 Akt2/Akt2(locked) Female 12.9 49 25 28 40 55
MAAE77.1d M00019653 Akt2/Akt2(locked) Female 12.8 58 31 31 49 58
MAAE62.1a M00019587 Akt2/Akt2(locked) Male 13.0 49 34 25 40 55
MAAE65.1a M00019606 Akt2/Akt2(locked) Male 12.8 52 22 25 43 49
MAAE65.1c M00019608 Akt2/Akt2(locked) Male 12.8 46 22 25 37 46
MAAE65.1d M00019609 Akt2/Akt2(locked) Male 12.8 61 31 34 46 61
MAAE71.1a M00019629 Akt2/Akt2(locked) Male 12.8 58 22 28 43 58
Akt2/Akt2 Average 12.77 52 25.33 26.67 42 55.67
Akt2/Akt2 Standard Deviation 0.16 5.29 4.99 3.77 3.74 4.85
Akt2/Akt2 Standard Error 0.05 1.76 1.66 1.26 1.25 1.62
Controls
Mouse Name Mouse Barcode Genotype Gender Age 6kHz 12kHz 18kHz 24kHz 30kHz
621.6d M00014134 +/+ Female 13.2 52 13 22 40 58
621.6e M00014135 +/+ Female 13.1 58 16 25 37 46
621.6f M00014136 +/+ Female 13.1 49 19 19 34 52
621.6g M00014137 +/+ Female 13.2 52 19 31 34 55
621.6h M00014138 +/+ Female 13.1 49 19 25 34 46
716.4c M00021245 +/+ Female 12.9 49 19 22 34 52
716.4d M00021247 +/+ Female 12.9 46 10 22 34 49
731.3b M00021246 +/+ Female 12.8 49 19 22 34 46
731.3c M00021248 +/+ Female 12.8 43 22 22 34 40
758.1c M00021249 +/+ Female 12.9 40 13 16 31 46
662.7a M00021253 +/+ Male 12.5 52 16 22 40 49
662.7b M00021255 +/+ Male 12.5 55 10 16 25 55
669.3a M00014124 +/+ Male 13.8 52 16 19 28 46
669.3b M00014125 +/+ Male 13.8 61 28 31 43 61
669.3c M00014126 +/+ Male 13.8 52 22 28 40 46
669.3d M00014127 +/+ Male 13.8 52 13 13 28 49
669.3e M00014128 +/+ Male 13.8 46 16 19 28 43
672.6b M00021254 +/+ Male 12.3 43 13 22 25 43
690.5a M00021250 +/+ Male 12.5 49 16 16 31 46
690.5b M00021251 +/+ Male 12.5 46 13 16 34 43
690.5c M00021252 +/+ Male 12.5 52 25 22 34 58
+/+ Average 13.04 49.86 17 21.43 33.43 49
+/+ Standard Deviation 0.49 4.82 4.57 4.69 4.78 5.55
+/+ Standard Error 0.11 1.05 1 1.02 1.04 1.21