Browsing Data by Marker Symbol: 'U'

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Ube2d4

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2d4 was found for the following reasons:

go_ontology	-	-amino acid ligase activity \| small conjugating protein ligase activity \| ubiquitin-protein ligase activity
go_term	-	ubiquitin-protein ligase activity
marker_name	-	ubiquitin-conjugating enzyme E2D 4
symbol	-	Ube2d4
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2d4
go	-	protein K27-linked ubiquitination
marker_symbol	-	Ube2d4
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	protein K27-linked ubiquitination

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2D 4 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	EG546638, Gm5959
Location:	Chr15:58846590-58847321(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:3644823	Ensembl Gene ID:	ENSMUSG00000061490	Vega Gene ID:
Entrez Gene ID:	546638	CCDS ID:

Interpro Families

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM	Vector - Initial Attempt Unsuccessful		Projects: 98292

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources no data available

Ube2dnl1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2dnl1 was found for the following reasons:

synonym	-	Ube2dnl
marker_name	-	ubiquitin-conjugating enzyme E2D N-terminal like 1
symbol	-	Ube2dnl1
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2dnl1
marker_symbol	-	Ube2dnl1
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2D N-terminal like 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	EG237009, Ube2dnl
Location:	ChrX:114905012-114905941(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:3646570	Ensembl Gene ID:	ENSMUSG00000050435	Vega Gene ID:	OTTMUSG00000018431
Entrez Gene ID:	237009	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2DNL	100131816

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM	Design Requested		Projects: 118031

	Vectors	ES Cells
KOMP-CSD	Vector - Initial Attempt Unsuccessful		Projects: 30125, 77700

	Vectors	ES Cells
KOMP-Regeneron	Design Not Possible		Projects: VG12531

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources no data available

Ube2dnl2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2dnl2 was found for the following reasons:

marker_name	-	ubiquitin-conjugating enzyme E2D N-terminal like 2
symbol	-	Ube2dnl2
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2dnl2
marker_symbol	-	Ube2dnl2
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2D N-terminal like 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	4930524E20Rik
Location:	ChrX:114907582-114908510(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1922347	Ensembl Gene ID:	ENSMUSG00000047079	Vega Gene ID:	OTTMUSG00000018419
Entrez Gene ID:	75097	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2D4	51619
Rat	Ube2d4l1	100359803

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD (Project: 67891)	order	order	view details

ES Cell Clones

Cassette Type

5 Deletion Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

DEPD00527_5_B02

DPGS00181_A_A07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1(KOMP)Mbp

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	-	3' Screen	no reads detected
Loss of WT Allele (LOA)	pass	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

DEPD00527_5_B03

DPGS00181_A_A07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1(KOMP)Mbp

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	-	3' Screen	no reads detected
Loss of WT Allele (LOA)	pass	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

DEPD00527_5_D01

DPGS00181_A_A07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1(KOMP)Mbp

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	-	3' Screen	no reads detected
Loss of WT Allele (LOA)	pass	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

DEPD00527_5_G01

DPGS00181_A_A07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1(KOMP)Mbp

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	-	3' Screen	no reads detected
Loss of WT Allele (LOA)	pass	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

DEPD00527_5_G04

DPGS00181_A_A07

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1(KOMP)Mbp

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	-	3' Screen	no reads detected
Loss of WT Allele (LOA)	pass	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
237612	DPGS00181_A_A07	PCS00181_A_A07	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

	Vectors	ES Cells
EUCOMM	Design Requested		Projects: 117516

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources no data available

Ube2e1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2e1 was found for the following reasons:

synonym	-	ubcM2
go_ontology	-	-amino acid ligase activity \| small conjugating protein ligase activity \| ubiquitin-protein ligase activity
go_term	-	ubiquitin-protein ligase activity
marker_name	-	ubiquitin-conjugating enzyme E2E 1
symbol	-	Ube2e1
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2e1
go	-	protein ubiquitination
marker_symbol	-	Ube2e1
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	protein ubiquitination

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2E 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	UbcM3, Ubce5, ubcM2
Location:	Chr14:18282722-18331859(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:107411	Ensembl Gene ID:	ENSMUSG00000021774	Vega Gene ID:	OTTMUSG00000028840
Entrez Gene ID:	22194	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2E1	7324

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 101591)	order	order	view details

ES Cell Clones

Cassette Type

5 Knockout-First Alleles

22 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0791_2_A10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_B09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_B10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_D09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_D11

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0791_2_A09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_A11

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_B12

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_C09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_C10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_C11

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_C12

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_D10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_E09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_E10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_E12

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_F09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_F10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_F11

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_F12

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_G09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_G10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_G11

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_G12

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_H09

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_H10

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0791_2_H11

PG00252_Z_7_C11

C57BL/6N-A^tm1Brd/a

JM8A3.N1.C2

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
380209	PG00252_Z_7_C11	PCS00252_A_G04	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
380209	PG00252_Z_7_C11	PCS00252_A_G04	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
NorCOMM	Design Requested		Projects: 66008

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
33	8	0	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ube2e2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2e2 was found for the following reasons:

synonym	-	Ube2e2
go_ontology	-	-amino acid ligase activity \| small conjugating protein ligase activity \| ubiquitin-protein ligase activity
go_term	-	ubiquitin-protein ligase activity
marker_name	-	ubiquitin-conjugating enzyme E2E 2
symbol	-	Ube2e2
interpro_desc	-	UBQ-conjugat_E2
microinjection_centre	-	UCD
marker_symbol_str	-	Ube2e2
go	-	protein K11-linked ubiquitination
marker_symbol	-	Ube2e2
microinjection_centre_status	-	UCD - Genotype confirmed
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	protein K11-linked ubiquitination

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2E 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	BC016265, Ube2e2
Location:	Chr14:18573575-18894267(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:2384997	Ensembl Gene ID:	ENSMUSG00000058317	Vega Gene ID:	OTTMUSG00000027804
Entrez Gene ID:	218793	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2E2	7325
Rat	Ube2e2	361013

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Ube2e2^{tm1a(KOMP)Wtsi}

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

BL1487

EPD0096_3_A11

C57BL/6NTac;C57BL/6NTac;C57BL/6N

view data
( about )

UCD

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	pass
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	pass
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	na	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD (Project: 38367)	order	order	view details

ES Cell Clones

Cassette Type

2 Knockout-First Alleles

0 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Targeting Vector:

genbank file | image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0096_3_A11

PRPGS00058_A_F07

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	pass	Vector Integrity	-

Distribution Centre
Karyotype	0.7 - 0.61	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0096_3_C12

PRPGS00058_A_F07

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	fail	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
45876	PRPGS00058_A_F07	PCS00058_A_F07	L1L2_Bact_P	L4L3_pD223_DTA_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
46	7	9	1	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ube2e3

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2e3 was found for the following reasons:

synonym	-	ubcM2
go_ontology	-	-amino acid ligase activity \| small conjugating protein ligase activity \| ubiquitin-protein ligase activity
go_term	-	ubiquitin-protein ligase activity
marker_name	-	ubiquitin-conjugating enzyme E2E 3
symbol	-	Ube2e3
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2e3
go	-	protein K11-linked ubiquitination
marker_symbol	-	Ube2e3
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	protein K11-linked ubiquitination

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2E 3 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	Ubce4, ubcM2
Location:	Chr2:78868124-78921293(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:107412	Ensembl Gene ID:	ENSMUSG00000027011	Vega Gene ID:	OTTMUSG00000016715
Entrez Gene ID:	22193	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2E3	10477
Rat	Ube2e3	295686

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM	Vector Unsuccessful - Project Terminated		Projects: 37128

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
25	4	1	0	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ube2f

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2f was found for the following reasons:

marker_name	-	ubiquitin-conjugating enzyme E2F (putative)
symbol	-	Ube2f
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2f
marker_symbol	-	Ube2f
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2F (putative) view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	2510010F15Rik, AI851109
Location:	Chr1:91250319-91286025(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1915171	Ensembl Gene ID:	ENSMUSG00000034343	Vega Gene ID:
Entrez Gene ID:	67921	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2F	140739
Rat	Ube2f	363284

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 99587)	order	order	view details

ES Cell Clones

Cassette Type

19 Knockout-First Alleles

4 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0820_4_A10

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	fail	LOXP	pass	LACZ	pass
Chromosome 1	pass	Chromosome 8a	pass	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	pass	Chromosome Y	pass

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_A11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_A12

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_B09

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_B11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_C10

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_D10

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_D11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_E10

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_E11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_E12

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_F09

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_F10

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_F12

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_G09

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_G12

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_H09

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_H10

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_H11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0820_4_B12

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_C09

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_C11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0820_4_G11

HTGR04022_Z_5_A11

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
375857	HTGR04022_Z_5_A11	PCS04022_A_E01	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
375857	HTGR04022_Z_5_A11	PCS04022_A_E01	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
NorCOMM	Design Requested		Projects: 70897

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
33	5	0	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ube2g1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2g1 was found for the following reasons:

go_ontology	-	-amino acid ligase activity \| small conjugating protein ligase activity \| ubiquitin-protein ligase activity
go_term	-	ubiquitin-protein ligase activity
marker_name	-	ubiquitin-conjugating enzyme E2G 1
symbol	-	Ube2g1
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2g1
go	-	protein K63-linked ubiquitination
marker_symbol	-	Ube2g1
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	protein K63-linked ubiquitination

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2G 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	2700059C12Rik, AI256795, AU014992, AW552068, D130023C12Rik
Location:	Chr11:72607283-72686481(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1914378	Ensembl Gene ID:	ENSMUSG00000020794	Vega Gene ID:	OTTMUSG00000006111
Entrez Gene ID:	67128	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2G1	7326
Rat	Ube2g1	64631

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 33172)	order	order	view details

ES Cell Clones

Cassette Type

0 Knockout-First Alleles

15 Targeted Non-Conditional Alleles

Promotorless

Targeting Vector:

genbank file | image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0590_5_A05

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_A06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_B04

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_B05

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_B06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_C04

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_C05

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_C06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_D04

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_D05

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_D06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_E06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_F06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_G04

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0590_5_H06

PG00011_A_2_E08

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotorless Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
39131	PG00011_A_2_E08	PCS00011_A_D08	L1L2_gt1	L3L4_pZero_DTA_kan	view

	Vectors	ES Cells
EUCOMM (Project: 89443)	order	order	view details

ES Cell Clones

Cassette Type

21 Knockout-First Alleles

6 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0712_8_A01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_A02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_A03

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_A04

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_B01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_B02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_B03

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_C01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_D01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_D02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_E02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_E04

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_F01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_F02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_F03

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_F04

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_G01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_G02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_G04

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_H01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_H02

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0712_8_B04

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_C03

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_D04

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_E01

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_E03

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0712_8_G03

HTGR03023_Z_3_B02

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
39131	HTGR03023_Z_3_B02	PCS03023_A_C07	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
39131	HTGR03023_Z_3_B02	PCS03023_A_C07	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
KOMP-Regeneron (Project: VG18683)	order	order	view details

ES Cell Clones

Cassette Type

1 Deletion Alleles

Promotor Driven

Allele:	genbank file
Targeting Vector:	genbank file

view all available ES cell clones

ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

18683A-B7

VG18683

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
	VG18683		ZEN-Ub1		view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
16	6	8	3	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ube2g2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2g2 was found for the following reasons:

synonym	-	UBC7
go_ontology	-	catabolic process \| ubiquitin-dependent protein catabolic process \| proteasomal ubiquitin-dependent protein
go_term	-	ubiquitin-dependent protein catabolic process
marker_name	-	ubiquitin-conjugating enzyme E2G 2
symbol	-	Ube2g2
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2g2
go	-	ubiquitin-dependent protein catabolic process
marker_symbol	-	Ube2g2
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	ubiquitin-dependent protein catabolic process

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2G 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	1110003O05Rik, D10Xrf369, UBC7, Ubc7p
Location:	Chr10:77622275-77645993(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1343188	Ensembl Gene ID:	ENSMUSG00000009293	Vega Gene ID:	OTTMUSG00000038229
Entrez Gene ID:	22213	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2G2	7327
Rat	Ube2g2	294331

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD (Project: 44641)	order	order	view details

ES Cell Clones

Cassette Type

8 Knockout-First Alleles

8 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0360_4_B05

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	pass	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_D07

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	pass	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_D08

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	pass	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_E05

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	pass	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_E07

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	pass	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_F08

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_G08

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_H06

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0360_4_A05

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_A06

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_C06

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_C08

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_D06

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_E08

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_F05

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0360_4_H08

PRPGS00159_A_C08

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
175331	PRPGS00159_A_C08	PCS00159_A_C08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
175331	PRPGS00159_A_C08	PCS00159_A_C08	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
26	2	1	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ube2h

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ube2h was found for the following reasons:

synonym	-	Ubc8
go_ontology	-	-amino acid ligase activity \| small conjugating protein ligase activity \| ubiquitin-protein ligase activity
go_term	-	ubiquitin-protein ligase activity
marker_name	-	ubiquitin-conjugating enzyme E2H
symbol	-	Ube2h
interpro_desc	-	UBQ-conjugat_E2
marker_symbol_str	-	Ube2h
go	-	protein K11-linked ubiquitination
marker_symbol	-	Ube2h
interpro	-	IPR000608\|\|UBQ-conjugat_E2\|\|Ubiquitin-conjugating enzyme, E2
GO	-	protein K11-linked ubiquitination

Gene Details

Marker Name(s):	ubiquitin-conjugating enzyme E2H view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	1500009C23Rik, AI181839, AI326965, AI462483, AW227540, E2-20K, Gid3, N28148, Ubc8
Location:	Chr6:30211289-30304539(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:104632	Ensembl Gene ID:	ENSMUSG00000039159	Vega Gene ID:	OTTMUSG00000014347
Entrez Gene ID:	22214	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	UBE2H	7328

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 83314)	order	order	view details

ES Cell Clones

Cassette Type

8 Knockout-First Alleles

13 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0679_4_B07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_B08

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_C05

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_D05

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_E08

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_F06

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_G07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_H05

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0679_4_A06

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_A07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_B05

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_B06

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_C06

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_C07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_C08

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_D06

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_D07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_E07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_F07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_H06

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0679_4_H07

HTGR03010_Z_4_H10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
43773	HTGR03010_Z_4_H10	PCS03010_A_D09	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
43773	HTGR03010_Z_4_H10	PCS03010_A_D09	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
NorCOMM	Vector Construction in Progress		Projects: 71354

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
40	11	1	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

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