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Mammalian Phenotype
Chromosome
Marker Symbol

Browsing Data by Marker Symbol: 'E'

Eya4

Eya4 was found for the following reasons:
ikmc_project_product_status - KOMP-CSD ES Cell Generated
marker_name - eyes absent 4 homolog (Drosophila)
product_status_str - ES Cell Generated
symbol - Eya4
interpro_desc - EYA
targeting_vector - HTGRS6004_A_E09
marker_symbol_str - Eya4
escell - EPD0585_2_E05
ensembl_gene_id - ENSMUSG00000010461
ensembl_peptide_id - ENSMUSP00000090335
marker_symbol - Eya4
product_status - ES Cell Generated
ensembl_transcript_id - ENSMUST00000092665
intermediate_vector - PCS6004_A_E09
allele - tm1e(KOMP)Wtsi
interpro - IPR006545||EYA||EYA

Gene Details

Marker Name(s): eyes absent 4 homolog (Drosophila)
view this gene in MGI
Marker Type: protein coding gene
Synonyms: B130023L16Rik
Location: Chr10:23104168-23349895(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1337104 Ensembl Gene ID: ENSMUSG00000010461 Vega Gene ID:
Entrez Gene ID: 14051 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human EYA4 2070
Rat Eya4 292172

WTSI Phenotyping no data available

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
currently unavailable Eya4tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) TCPA0076 EPD0585_2_D08 C57BL/6NCrl;B6N-Tyrc/BrdCrCrl;C57BL/6N-Atm1Brd/a view data
about )
TCP/
Southern Blot na TV Backbone Assay na 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) pass
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 79924)
order order view details

ES Cell Clones

Cassette Type 10 Knockout-First Alleles 2 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0585_2_A07 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 0.8 - 0.71 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_A08 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_B07 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA fail LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_D07 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_D08 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_D08 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 0.6 - 0.51 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_E05 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_E07 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_F07 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_F08 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0585_2_C05 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0585_2_G05 HTGRS6004_A_E09 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
94693 HTGRS6004_A_E09 PCS6004_A_E09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
94693 HTGRS6004_A_E09 PCS6004_A_E09 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
Vectors ES Cells
KOMP-Regeneron
Design Not Possible Projects: VG15962

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
74 10 10 2 view in Ensembl

Ezg

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Ezg was found for the following reasons:
marker_name - extent of zona glomerulosa
symbol - Ezg
marker_symbol_str - Ezg
marker_symbol - Ezg

Gene Details

Marker Name(s): extent of zona glomerulosa
view this gene in MGI
Marker Type: heritable phenotypic marker
Synonyms:
Location: ChrUN
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:95472 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: 104375 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

Ezh1

Ezh1 was found for the following reasons:
colony_prefix - Ezh1 EPD0188_1_E12
ikmc_project_product_status - EUCOMM ES Cell Generated
marker_name - enhancer of zeste homolog 1 (Drosophila)
product_status_str - ES Cell Generated
symbol - Ezh1
interpro_desc - EZH2_WD-Binding
marker_symbol_str - Ezh1
escell - EPD0188_1_E12
ensembl_gene_id - ENSMUSG00000006920
ensembl_peptide_id - ENSMUSP00000102905
marker_symbol - Ezh1
product_status - ES Cell Generated
ensembl_transcript_id - ENSMUST00000107284
ikmc_project_product_status_str - EUCOMM Vector Generated
ikmc_project - EUCOMM
allele - tm1e(EUCOMM)Wtsi
interpro - IPR021654||EZH2_WD-Binding||WD repeat binding protein EZH2

Gene Details

Marker Name(s): enhancer of zeste homolog 1 (Drosophila)
view this gene in MGI
Marker Type: protein coding gene
Synonyms: AW557915
Location: Chr11:101191115-101226463(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1097695 Ensembl Gene ID: ENSMUSG00000006920 Vega Gene ID: OTTMUSG00000002729
Entrez Gene ID: 14055 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human EZH1 2145
Rat Ezh1 303547

WTSI Phenotyping no data available

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
currently unavailable Ezh1tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) Ezh1 EPD0188_1_E12 EPD0188_1_E12 C57BL/6N;C57BL/6N;C57BL/6N view data
about )
MARC/
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
EUCOMM
(Project: 37205)
order order view details

ES Cell Clones

Cassette Type 8 Knockout-First Alleles 1 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0188_1_A09 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_C11 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_D09 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot pass Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_E12 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_F10 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_G12 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_H09 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR pass 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0188_1_H10 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1a(EUCOMM)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0188_1_A10 PRPGS00086_A_C02 C57BL/6N JM8.N4 tm1e(EUCOMM)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
87984 PRPGS00086_A_C02 PCS00086_A_C02 L1L2_Bact_P L4L3_pD223_DTA_spec view
87984 PRPGS00086_A_C02 PCS00086_A_C02 L1L2_Bact_P L4L3_pD223_DTA_spec view
Vectors ES Cells
KOMP-Regeneron
Parental BAC Obtained Projects: VG18052

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
29 5 1 2 view in Ensembl

Ezh2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Ezh2 was found for the following reasons:
synonym - Enx-1
ikmc_project_product_status - EUCOMM ES Cell Generated
marker_name - enhancer of zeste homolog 2 (Drosophila)
product_status_str - ES Cell Generated
symbol - Ezh2
interpro_desc - EZH2_WD-Binding
targeting_vector - PGS00029_A_E07
marker_symbol_str - Ezh2
escell - EPD0052_2_E05
ensembl_gene_id - ENSMUSG00000029687
ensembl_peptide_id - ENSMUSP00000080419
marker_symbol - Ezh2
product_status - ES Cell Generated
ensembl_transcript_id - ENSMUST00000081721
intermediate_vector - PCS00029_A_E07
mp_ontology - behavior/neurological phenotype | abnormal behavior | abnormal eating/drinking behavior | abnormal
ikmc_project_product_status_str - EUCOMM Vector Generated
ikmc_project - EUCOMM
allele - tm1e(EUCOMM)Hmgu
mp_term - abnormal eating/drinking behavior
interpro - IPR021654||EZH2_WD-Binding||WD repeat binding protein EZH2

Gene Details no data available

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Ezh2tm1a(EUCOMM)Wtsi MALM view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Ezh2tm1a(EUCOMM)Wtsi MALM view view view view view view view view view view view view view view view view view view view view view view view pdf view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

IKMC Vectors and ES Cells no data available

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
16 5 5 0 view in Ensembl

Ezr

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
Ezr was found for the following reasons:
synonym - ezrin
ikmc_project_product_status - EUCOMM ES Cell Generated
marker_name - ezrin
product_status_str - ES Cell Generated
symbol - Ezr
interpro_desc - Ezrin/radixin/moesin
marker_symbol_str - Ezr
escell - EPD0080_2_E08
ensembl_gene_id - ENSMUSG00000052397
ensembl_peptide_id - ENSMUSP00000063734
marker_symbol - Ezr
product_status - ES Cell Generated
ensembl_transcript_id - ENSMUST00000064234
mp_ontology - | abnormal embryogenesis/ development | prenatal growth retardation | abnormal embryonic growth
ikmc_project_product_status_str - EUCOMM Vector Generated
ikmc_project - EUCOMM
allele - tm1e(EUCOMM)Wtsi
ikmc_project_id - IST10808E12
mp_term - embryonic growth retardation
interpro - IPR011174||ERM||Ezrin/radixin/moesin

Gene Details no data available

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
Ezrtm2a(EUCOMM)Wtsi MDYB view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
Ezrtm2a(EUCOMM)Wtsi MDYB view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

IKMC Vectors and ES Cells no data available

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
24 6 0 0 view in Ensembl

e9.58a

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
e9.58a was found for the following reasons:
marker_name - e9.5 arrest 8a
symbol - e9.58a
marker_symbol_str - e9.58a
marker_symbol - e9.58a

Gene Details no data available

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

e9.5a19c

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
e9.5a19c was found for the following reasons:
marker_name - e9.5 arrest 19c
symbol - e9.5a19c
marker_symbol_str - e9.5a19c
marker_symbol - e9.5a19c

Gene Details

Marker Name(s): e9.5 arrest 19c
view this gene in MGI
Marker Type: heritable phenotypic marker
Synonyms:
Location: Chr16
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:3578209 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

ebo

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
ebo was found for the following reasons:
marker_name - ebouriffe
symbol - ebo
marker_symbol_str - ebo
marker_symbol - ebo

Gene Details

Marker Name(s): ebouriffe
view this gene in MGI
Marker Type: heritable phenotypic marker
Synonyms:
Location: Chr2
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:107466 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: 13594 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

ec

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
ec was found for the following reasons:
marker_name - ectopic
symbol - ec
marker_symbol_str - ec
marker_symbol - ec

Gene Details

Marker Name(s): ectopic
view this gene in MGI
Marker Type: heritable phenotypic marker
Synonyms:
Location: ChrUN
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:95277 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available

elap

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources
elap was found for the following reasons:
marker_name - eye lens aplasia
symbol - elap
marker_symbol_str - elap
marker_symbol - elap

Gene Details

Marker Name(s): eye lens aplasia
view this gene in MGI
Marker Type: heritable phenotypic marker
Synonyms: lap
Location: Chr2
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:105916 Ensembl Gene ID: Vega Gene ID:
Entrez Gene ID: 13708 CCDS ID:

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells no data available

DNA Clone Resources no data available