Browsing Data by Available Products: 'TIGM Mice'

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Mammalian Phenotype

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Browsing Data by Available Products: 'TIGM Mice'

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Dusp12

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Dusp12 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	dual specificity phosphatase 12 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	1190004O14Rik, AA027408, AW049275, ENSMUSG00000045460, ESTM36, LMW-DSP4, T-DSP4, VH1, mVH1
Location:	Chr1:170873498-170885540(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1890614	Ensembl Gene ID:	ENSMUSG00000026659	Vega Gene ID:	OTTMUSG00000035839
Entrez Gene ID:	80915	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	DUSP12	11266
Rat	Dusp12	64014

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD (Project: 47601)	order	order	view details

ES Cell Clones

Cassette Type

2 Knockout-First Alleles

0 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Targeting Vector:

genbank file | image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0208_6_A05

PRPGS00093_A_D12

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	pass	Vector Integrity	pass

Distribution Centre
Karyotype	-	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	fail	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0208_6_B06

PRPGS00093_A_D12

C57BL/6N

JM8.N4

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	pass	Vector Integrity	pass

Distribution Centre
Karyotype	-	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	fail	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
93946	PRPGS00093_A_D12	PCS00093_B_D12	L1L2_Bact_P	L4L3_pD223_DTA_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
17	2	0	1	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

F2rl1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

F2rl1 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	coagulation factor II (thrombin) receptor-like 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	Gpcr11, PAR-2, Par2, Protease-activated receptor-2, proteinase-activated receptor-2
Location:	Chr13:95511732-95525240(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:101910	Ensembl Gene ID:	ENSMUSG00000021678	Vega Gene ID:
Entrez Gene ID:	14063	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	F2RL1	2150
Rat	F2rl1	116677

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

currently unavailable

F2rl1^{tm1a(EUCOMM)Wtsi}

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

EM:06771

P4161-EPD0592_3_B02

EPD0592_3_B02

C57BL/6NTac;C57BL/6NTac;C57BL/6N-A^tm1Brd/a

view data
( about )

ICS/

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	pass	5' Cassette Integrity		Neo SR-PCR	pass
Mutant Specific SR-PCR	pass	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 78671)	order	order	view details

ES Cell Clones

Cassette Type

13 Knockout-First Alleles

11 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0592_3_A02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_A03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_B02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	pass
3' LR-PCR	-

EPD0592_3_B04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_C02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_C04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_D02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	pass	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	pass
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	pass
3' LR-PCR	-

EPD0592_3_E04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	pass	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_F01

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_G02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_G04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_H01

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	pass	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_H03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0592_3_A04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_B03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_D03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_D04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_E02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_E03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_F03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_G01

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_G03

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_H02

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0592_3_H04

PG00202_Z_5_A04

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
87373	PG00202_Z_5_A04	PCS00202_A_E12	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
87373	PG00202_Z_5_A04	PCS00202_A_E12	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
30	4	1	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Fads1

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Fads1 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	fatty acid desaturase 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	0710001O03Rik, A930006B21Rik, AI317215
Location:	Chr19:10182888-10196870(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1923517	Ensembl Gene ID:	ENSMUSG00000010663	Vega Gene ID:
Entrez Gene ID:	76267	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	FADS1	3992
Rat	Fads1	84575

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

currently unavailable

Fads1^{tm1(KOMP)Vlcg}

Deletion (Promotor Driven Cassette)

BL3530

17259A-G2

C57BL/6NCrl;C57BL/6NCrl;C57BL/6N

view data
( about )

UCD/

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	na
Mutant Specific SR-PCR	na	LoxP Confirmation	na	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-Regeneron (Project: VG17259)	order	order	view details

ES Cell Clones

Cassette Type

6 Deletion Alleles

Promotor Driven

Allele:	genbank file
Targeting Vector:	genbank file

view all available ES cell clones

ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

17259A-A3

VG17259

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17259A-B1

VG17259

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17259A-B2

VG17259

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17259A-D12

VG17259

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17259A-F2

VG17259

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

17259A-G2

VG17259

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
	VG17259		ZEN-Ub1		view

	Vectors	ES Cells
EUCOMM (Project: 48623)	order	order	view details

ES Cell Clones

Cassette Type

13 Knockout-First Alleles

23 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0548_9_A07

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_A08

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_A11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	pass
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_B07

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_B08

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_B11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_B12

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_C07

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_C08

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_C12

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_D08

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_E10

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_F10

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0548_9_A09

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_B10

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_C10

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_D09

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_D10

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_D11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_D12

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_E08

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_E09

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_E11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_F07

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_F08

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_F09

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_F11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_F12

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_G09

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_G11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_G12

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_H09

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_H10

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_H11

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0548_9_H12

PRPGS00130_B_H10

C57BL/6N

JM8.N4

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0570_2_D02

PRPGS00130_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm2e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
115576	PRPGS00130_B_H10	PCS00130_B_H10	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
115576	PRPGS00130_B_H10	PCS00130_B_H10	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
34	4	0	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Fam76b

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Fam76b was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	family with sequence similarity 76, member B view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	2810485I05Rik, C78303
Location:	Chr9:13827716-13846541(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1920076	Ensembl Gene ID:	ENSMUSG00000037808	Vega Gene ID:	OTTMUSG00000023308
Entrez Gene ID:	72826	CCDS ID:

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	FAM76B	143684

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 78630)	order	order	view details

ES Cell Clones

Cassette Type

4 Knockout-First Alleles

2 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0591_5_A11

PG00202_Z_3_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0591_5_A12

PG00202_Z_3_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0591_5_F09

PG00202_Z_3_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0591_5_F10

PG00202_Z_3_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0591_5_D09

PG00202_Z_3_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0591_5_D10

PG00202_Z_3_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
55358	PG00202_Z_3_F10	PCS00202_A_C02	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
55358	PG00202_Z_3_F10	PCS00202_A_C02	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
KOMP-Regeneron (Project: VG19126)	order	order	view details

ES Cell Clones

Cassette Type

2 Deletion Alleles

Promotor Driven

Allele:	genbank file
Targeting Vector:	genbank file

view all available ES cell clones

ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

19126A-B1

VG19126

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

19126A-B9

VG19126

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
	VG19126		ZEN-Ub1		view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources no data available

Frs3	Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Frs3 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	fibroblast growth factor receptor substrate 3 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	4930417B13Rik, AI449674, Frs2beta, SNT2, Snt2
Location:	Chr17:47689030-47704286(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:2135965	Ensembl Gene ID:	ENSMUSG00000023266	Vega Gene ID:	OTTMUSG00000018351
Entrez Gene ID:	107971	CCDS ID:

Interpro Families

Insulin Receptor Substrate 1, Ptb (IPR002404)

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	FRS3	10817
Rat	Frs3	316213

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 76842)	order	order	view details

ES Cell Clones

Cassette Type

3 Knockout-First Alleles

21 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0665_5_D10

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_E11

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0666_4_B03

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	pass	LOXP	pass	LACZ	pass
Chromosome 1	pass	Chromosome 8a	pass	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	pass	Chromosome Y	pass

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0665_6_A09

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_A10

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_A11

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_A12

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_B09

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_B12

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_C10

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_C11

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_C12

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_D09

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_E09

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_E10

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_F09

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_F10

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_F11

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_F12

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_G10

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_G11

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_G12

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_H11

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0665_6_H12

PG00195_Z_7_F10

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
79136	PG00195_Z_7_F10	PCS00195_A_F11	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
79136	PG00195_Z_7_F10	PCS00195_A_F11	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

	Vectors	ES Cells
KOMP-Regeneron (Project: VG19352)	order	order	view details

ES Cell Clones

Cassette Type

2 Deletion Alleles

Promotor Driven

Allele:	genbank file
Targeting Vector:	genbank file

view all available ES cell clones

ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

19352A-A9

VG19352

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

19352A-D5

VG19352

C57BL/6NTac

VGB6

tm1(KOMP)Vlcg

Deletion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	-	LoxP Screen	-	3' Screen	-
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
	VG19352		ZEN-Ub1		view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
24	7	1	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Galnt2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Galnt2 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	AI480629, ppGaNTase-T2
Location:	Chr8:124231391-124345724(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:894694	Ensembl Gene ID:	ENSMUSG00000089704	Vega Gene ID:	OTTMUSG00000037046
Entrez Gene ID:	108148	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	GALNT2	2590
Rat	Galnt2	292090

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD	Vector - Initial Attempt Unsuccessful		Projects: 28550, 69983

	Vectors	ES Cells
KOMP-Regeneron	Targeting Vector QC Completed		Projects: VG19744

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
33	13	8	0	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Ggnbp2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Ggnbp2 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	gametogenetin binding protein 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	AI451896, C75939, D330017P12Rik, DIF-3, Zfp403
Location:	Chr11:84832361-84870817(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:2387356	Ensembl Gene ID:	ENSMUSG00000020530	Vega Gene ID:	OTTMUSG00000001025
Entrez Gene ID:	217039	CCDS ID:

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	GGNBP2	79893
Rat	Ggnbp2	360584

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

currently unavailable

Ggnbp2^{tm1a(KOMP)Wtsi}

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

TCPA0058

EPD0553_4_A06

C57BL/6NCrl;B6N-Tyrc/BrdCrCrl;C57BL/6N-A^tm1Brd/a

view data
( about )

TCP

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	pass
Loss of WT Allele (LOA) qPCR	pass	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	pass
LacZ SR-PCR	pass	5' Cassette Integrity		Neo SR-PCR	pass
Mutant Specific SR-PCR	pass	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

IKMC Vectors and ES Cells

	Vectors	ES Cells
KOMP-CSD (Project: 46209)	order	order	view details

ES Cell Clones

Cassette Type

23 Knockout-First Alleles

6 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0553_4_A06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	pass

Distribution Centre
Karyotype	0.7 - 0.61	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_A07

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	pass

Distribution Centre
Karyotype	0.6 - 0.51	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_A08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	pass

Distribution Centre
Karyotype	0.6 - 0.51	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_B05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_B06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_B07

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_B08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_C06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_C07

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_C08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_D05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_D06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_D07

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_D08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_E05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_E07

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_E08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_F06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_F08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_G06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_G08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_H05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_H08

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(KOMP)Wtsi

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

EPD0553_4_A05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	pass

Distribution Centre
Karyotype	-	Copy Number	pass	Cells Thawed Correctly	-
5' LR-PCR	pass	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_C05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_E06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_G05

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_H06

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

EPD0553_4_H07

PG00131_Z_3_F05

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(KOMP)Wtsi

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not attempted	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
191009	PG00131_Z_3_F05	PCS00131_A_F05	L1L2_Bact_P	L3L4_pZero_DTA_kan	view
191009	PG00131_Z_3_F05	PCS00131_A_F05	L1L2_Bact_P	L3L4_pZero_DTA_kan	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
23	3	2	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Glipr2

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Glipr2 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	GLI pathogenesis-related 2 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	5730414A08Rik, C77180, GAPR-1
Location:	Chr4:43957401-43979118(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1917770	Ensembl Gene ID:	ENSMUSG00000028480	Vega Gene ID:	OTTMUSG00000007145
Entrez Gene ID:	384009	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	GLIPR2	152007
Rat	Glipr2	679819

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM	Vector Unsuccessful - Project Terminated		Projects: 100092, 82777, 38318, 51876

	Vectors	ES Cells
KOMP-Regeneron	Parental BAC Obtained		Projects: VG11971

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
28	4	2	0	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Gpr1	Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Gpr1 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	G protein-coupled receptor 1 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:
Location:	Chr1:63182691-63214543(-)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:2385324	Ensembl Gene ID:	ENSMUSG00000046856	Vega Gene ID:	OTTMUSG00000002612
Entrez Gene ID:	241070	CCDS ID:

Interpro Families

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	GPR1	2825
Rat	Gpr1	25457

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice

Allele Name

Allele Type

EMMA ID

Colony Prefix

ES Cell/Common Name

Genetic Background

QC/Screening Data

MI/Distribution Centre

contact distributor

Gpr1^{tm1a(EUCOMM)Hmgu}

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

EM:04737

Monterotondo-HEPD0551_8_C01-1

HEPD0551_8_C01

C57BL/6NTac;C57BL/6NTac;C57BL/6N-A^tm1Brd/a

view data
( about )

Monterotondo

Southern Blot	na	TV Backbone Assay	na	5' LR-PCR	na
Loss of WT Allele (LOA) qPCR	na	Homozygous Loss of WT Allele (LOA) SR-PCR	na	Neo Count (qPCR)	na
LacZ SR-PCR	na	5' Cassette Integrity		Neo SR-PCR	pass
Mutant Specific SR-PCR	na	LoxP Confirmation	pass	3' LR-PCR	na
Genotyping Comment

view original data

Data provided by The International Knockout Mouse Consortium (IKMC), The European Mouse Mutant Archive (EMMA)

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 66732)	order	order	view details

ES Cell Clones

Cassette Type

9 Knockout-First Alleles

14 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0551_8_B04

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_C01

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	pass	Map Test	-	Karyotype	-
TV Backbone Assay	pass	5' LR-PCR	pass	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	pass	Mutant Specific SR-PCR	pass	LoxP Confirmation	pass
3' LR-PCR	pass

HEPD0551_8_D02

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_D04

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_E02

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_E03

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_F02

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_G02

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_H03

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0551_8_A03

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_B06

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_C02

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_C03

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_C06

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_D01

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_D06

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_E06

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_F01

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_F04

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_G01

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_G04

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_H04

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0551_8_H06

PRPGS00135_B_H10

C57BL/6N-A^tm1Brd/a

JM8A1.N3

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	not confirmed
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
209430	PRPGS00135_B_H10	PCS00135_C_H10	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view
209430	PRPGS00135_B_H10	PCS00135_C_H10	L1L2_Bact_P	R3R4_pBR_DTA+_Bsd_amp	view

	Vectors	ES Cells
KOMP-Regeneron	Parental BAC Obtained		Projects: VG13236

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
16	4	1	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

Gpr160

Datasets: Gene Details WTSI Phenotyping Mice IKMC Vectors and ES Cells DNA Clone Resources

Gpr160 was found for the following reasons:

ikmc_project_product_status_str	-	TIGM Mouse Available

Gene Details

Marker Name(s):	G protein-coupled receptor 160 view this gene in MGI
Marker Type:	protein coding gene
Synonyms:	1700025D19Rik
Location:	Chr3:30855950-30897190(+)

Related Human Conditions (from OMIM) - no related Human Condition

More Information

MGI Gene Accession:	MGI:1919112	Ensembl Gene ID:	ENSMUSG00000037661	Vega Gene ID:	OTTMUSG00000022043
Entrez Gene ID:	71862	CCDS ID:

Interpro Families

Gpcr, Rhodopsin Like, 7 Tm (IPR017452)

Orthologs / Homologs

Species	Marker Symbol	Entrez Gene ID	Ensembl Gene ID
Human	GPR160	26996
Rat	Gpr160	499588

view original data

Data provided by Mouse Genome Informatics (MGI), Ensembl

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

	Vectors	ES Cells
EUCOMM (Project: 89102)	order	order	view details

ES Cell Clones

Cassette Type

14 Knockout-First Alleles

12 Targeted Non-Conditional Alleles

Promotor Driven

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

Allele:	genbank file \| image
Targeting Vector:	genbank file \| image

view all available ES cell clones

ES Cell Clones With Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0731_6_A01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_B03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_C02

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_D01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_D03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_E01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_E02

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_E03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_F01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_F02

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_F03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	not confirmed	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_F04

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_G04

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_H01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1a(EUCOMM)Hmgu

Knockout-First - Reporter Tagged Insertion (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	pass	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

ES Cell Clones Without Conditional Potential

ES Cell Clone

Targeting Vector

ES Cell Strain

ES Cell Line

Allele

Allele Type

QC/Screening Data

HEPD0731_6_A02

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_A03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_B01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_B04

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_C01

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_C03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_C04

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_D02

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_E04

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_G03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_H03

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

HEPD0731_6_H04

HTGR03018_Z_6_E06

C57BL/6N-A^tm1Brd/a

JM8A3.N1

tm1e(EUCOMM)Hmgu

Targeted Non-Conditional (Promotor Driven Cassette)

view data ( about )

Production Centre
5' Screen	pass	LoxP Screen	not confirmed	3' Screen	pass
Loss of WT Allele (LOA)	-	Vector Integrity	-

Distribution Centre
Karyotype	-	Copy Number	-	Cells Thawed Correctly	-
5' LR-PCR	-	5' SR-PCR	-	3' SR-PCR	-
LOA	-	LOXP	-	LACZ	-
Chromosome 1	-	Chromosome 8a	-	Chromosome 8b	-
Chromosome 11a	-	Chromosome 11b	-	Chromosome Y	-

User/Mouse Clinic
Southern Blot	-	Map Test	-	Karyotype	-
TV Backbone Assay	-	5' LR-PCR	-	Loss of WT Allele (LOA)	-
Neo Count (qPCR)	-	LacZ SR-PCR	-	5' Cassette Integrity	-
Neo SR-PCR	-	Mutant Specific SR-PCR	-	LoxP Confirmation	-
3' LR-PCR	-

Targeting Vectors

Design	Targeting Vector	Intermediate Vector	Cassette	Backbone	Targeting Vector Genbank File
114916	HTGR03018_Z_6_E06	PCS03018_A_F12	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view
114916	HTGR03018_Z_6_E06	PCS03018_A_F12	L1L2_Bact_P	L3L4_pD223_DTA_T_spec	view

view original data

Data provided by The International Knockout Mouse Consortium (IKMC)

DNA Clone Resources

C57Bl/6J Bacs	129 Bacs	MICER clones	KO Targeting Vectors
30	4	3	2	view in Ensembl

view original data

Data provided by The Wellcome Trust Sanger Institute (WTSI)

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Mouse Resources Portal

Available Products

Mammalian Phenotype

Chromosome

Marker Symbol

Browsing Data by Available Products: 'TIGM Mice'

Dusp12

Dusp12 was found for the following reasons:

Gene Details

Related Human Conditions (from OMIM) - no related Human Condition

More Information

Interpro Families

Orthologs / Homologs

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Links

ES Cell Clones

ES Cell Clones With Conditional Potential

ES Cell Clones Without Conditional Potential

Targeting Vectors

DNA Clone Resources

F2rl1

F2rl1 was found for the following reasons:

Gene Details

Related Human Conditions (from OMIM) - no related Human Condition

More Information

Interpro Families

Orthologs / Homologs

WTSI Phenotyping no data available

Mice

IKMC Vectors and ES Cells

Links

ES Cell Clones

ES Cell Clones With Conditional Potential

ES Cell Clones Without Conditional Potential

Targeting Vectors

DNA Clone Resources

Fads1

Fads1 was found for the following reasons:

Gene Details

Related Human Conditions (from OMIM) - no related Human Condition

More Information

Interpro Families

Orthologs / Homologs

WTSI Phenotyping no data available

Mice

IKMC Vectors and ES Cells

Links

ES Cell Clones

ES Cell Clones With Conditional Potential

ES Cell Clones Without Conditional Potential

Targeting Vectors

Links

ES Cell Clones

ES Cell Clones With Conditional Potential

ES Cell Clones Without Conditional Potential

Targeting Vectors

DNA Clone Resources

Fam76b

Fam76b was found for the following reasons:

Gene Details

Related Human Conditions (from OMIM) - no related Human Condition

More Information

Orthologs / Homologs

WTSI Phenotyping no data available

Mice no data available

IKMC Vectors and ES Cells

Links

ES Cell Clones

ES Cell Clones With Conditional Potential

ES Cell Clones Without Conditional Potential

Targeting Vectors

Links

ES Cell Clones

ES Cell Clones With Conditional Potential

ES Cell Clones Without Conditional Potential

Targeting Vectors

DNA Clone Resources no data available

Frs3