Available Products
Mammalian Phenotype
Chromosome
Marker Symbol

Browsing Data by Available Products: 'KOMP-CSD Mice'

2310057J18Rik

2310057J18Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 2310057J18 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms: 2210414P04Rik
Location: Chr10:28972288-28986303(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1914969 Ensembl Gene ID: ENSMUSG00000015519 Vega Gene ID: OTTMUSG00000028053
Entrez Gene ID: 67719 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Rat RGD1311933 308056

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
2310057J18Riktm1a(KOMP)Wtsi MCSX C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
2310057J18Riktm1a(KOMP)Wtsi MCSX C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view pdf view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 2310057J18Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MCSX EPD0101_4_E03 C57BL/6NTac;C57BL/6NTac;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 28704)
order order view details

ES Cell Clones

Cassette Type 1 Knockout-First Alleles 1 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0101_4_E03 PRPGS00059_A_B11 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0101_4_E04 PRPGS00059_A_B11 C57BL/6N JM8.F6 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
46093 PRPGS00059_A_B11 PCS00059_A_B11 L1L2_Bact_P L4L3_pD223_DTA_spec view
46093 PRPGS00059_A_B11 PCS00059_A_B11 L1L2_Bact_P L4L3_pD223_DTA_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
28 5 1 2 view in Ensembl

2610002J02Rik

2610002J02Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 2610002J02 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms:
Location: Chr4:155249802-155256684(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1914763 Ensembl Gene ID: ENSMUSG00000073684 Vega Gene ID: OTTMUSG00000010597
Entrez Gene ID: 67513 CCDS ID:
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human C1orf86 199990
Rat RGD1308923 362678

WTSI Phenotyping no data available

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 2610002J02Riktm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) BL1947 DEPD00507_1_A09 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
UCD/KOMP Repo
Southern Blot na TV Backbone Assay na 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) pass
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 23160)
order order view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 23 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
DEPD00507_1_A09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype 0.9 - 0.81 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_A10 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_A11 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_A12 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_B09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_B11 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_B12 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_C09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_C10 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_C11 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_D09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_D11 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_D12 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_E11 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_E12 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_F09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_F10 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_F11 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_F12 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_G09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_G10 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_G12 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
DEPD00507_1_H09 DPGS00179_A_H04 C57BL/6N-Atm1Brd/a JM8A3 tm1(KOMP)Mbp Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen - 3' Screen no reads detected
Loss of WT Allele (LOA) pass Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
236999 DPGS00179_A_H04 PCS00179_A_H04 L1L2_Bact_P R3R4_pBR_DTA+_Bsd_amp view
Vectors ES Cells
KOMP-Regeneron
(Project: VG11629)
order order view details

ES Cell Clones

Cassette Type 0 Knockout-First Alleles 6 Targeted Non-Conditional Alleles
Promotor Driven
Targeting Vector: genbank file
Allele: genbank file
Targeting Vector: genbank file
ES Cell Clones With Conditional Potential

No clones available.

ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
11629A-A2 VG11629 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
11629A-C2 VG11629 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
11629A-E1 VG11629 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
11629A-F9 VG11629 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
11629A-G6 VG11629 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
11629A-H2 VG11629 C57BL/6NTac VGB6 tm1(KOMP)Vlcg Reporter-Tagged Deletion (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen - LoxP Screen - 3' Screen -
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
VG11629 ZEN-UB1.GB view
Vectors ES Cells
EUCOMM
Vector Construction in Progress Projects: 117247

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
13 5 0 1 view in Ensembl

3110035E14Rik

3110035E14Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 3110035E14 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms: AI415019, AV164974, R75066
Location: Chr1:9601199-9627143(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1924232 Ensembl Gene ID: ENSMUSG00000067879 Vega Gene ID: OTTMUSG00000022603
Entrez Gene ID: 76982 CCDS ID:
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human C8orf46 254778
Rat RGD1561849 500393

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
3110035E14Riktm1a(KOMP)Wtsi MELP C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view
3110035E14Rik MUAH view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
3110035E14Riktm1a(KOMP)Wtsi MELP C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view view
3110035E14Rik MUAH view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
currently unavailable 3110035E14Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MELP EPD0684_4_A05 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment
currently unavailable 3110035E14Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) TCPA0050 EPD0684_4_A05 C57BL/6NCrl;B6N-Tyrc/BrdCrCrl;C57BL/6N-Atm1Brd/a view data
about )
TCP
Southern Blot na TV Backbone Assay na 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 32518)
order order view details

ES Cell Clones

Cassette Type 7 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0684_4_A05 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 0.6 - 0.51 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0684_4_A06 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number fail Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0684_4_C08 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0684_4_D07 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0684_4_E05 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0684_4_E06 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0684_4_H06 PG00028_Y_5_E08 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 0.8 - 0.71 Copy Number pass Cells Thawed Correctly -
5' LR-PCR fail 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
41531 PG00028_Y_5_E08 PCS00028_A_E08 L1L2_Bact_P L3L4_pD223_DTA_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
24 8 1 1 view in Ensembl

3830417A13Rik

3830417A13Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 3830417A13 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms:
Location: ChrX:64173548-64178778(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1917946 Ensembl Gene ID: ENSMUSG00000031179 Vega Gene ID: OTTMUSG00000017599
Entrez Gene ID: 70696 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Rat MGC114427 317297

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
3830417A13Riktm1a(KOMP)Wtsi MECY C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
3830417A13Riktm1a(KOMP)Wtsi MECY C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 3830417A13Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MECY EPD0658_2_H08 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 24958)
order order view details

ES Cell Clones

Cassette Type 18 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0658_2_A06 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_A07 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_B06 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_B08 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_C05 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_C07 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_C08 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_D07 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_D08 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_E07 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_E08 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_F05 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_G05 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_G07 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_G08 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_H05 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_H07 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0658_2_H08 PG00078_Z_1_D01 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR pass Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
55738 PG00078_Z_1_D01 PCS00078_A_D01 L1L2_Bact_P L3L4_pD223_DTA_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
20 4 0 1 view in Ensembl

4921536K21Rik

4921536K21Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 4921536K21 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms: AV043724
Location: Chr11:3886088-3895098(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1914680 Ensembl Gene ID: ENSMUSG00000020434 Vega Gene ID: OTTMUSG00000005036
Entrez Gene ID: 67430 CCDS ID:
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human C5orf52 100190949
Rat RGD1308775 305476

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
4921536K21Riktm1a(KOMP)Wtsi MFCN C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
4921536K21Riktm1a(KOMP)Wtsi MFCN C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 4921536K21Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MFCN EPD0218_5_D09 C57BL/6NTac;C57BL/6NTac;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 34319)
order order view details

ES Cell Clones

Cassette Type 13 Knockout-First Alleles 9 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0218_1_G09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_4_A12 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_4_F11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_4_G12 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_B09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_C10 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_D09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 fail Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_D11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_E09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_E11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_F09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_F10 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_H11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0218_1_B12 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_1_D11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_1_E11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_1_F12 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_1_G10 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_4_D09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_4_D11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_4_G11 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0218_5_A09 PRPGS00048_B_A06 C57BL/6N JM8.N4 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
43838 PRPGS00048_B_A06 PCS00048_A_A06 L1L2_Bact_P L3L4_pD223_DTA_spec view
43838 PRPGS00048_B_A06 PCS00048_A_A06 L1L2_Bact_P L3L4_pD223_DTA_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
15 2 0 2 view in Ensembl

4930591A17Rik

4930591A17Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 4930591A17 gene
view this gene in MGI
Marker Type: lincRNA gene
Synonyms:
Location: Chr2:179414936-179416882(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1915425 Ensembl Gene ID: ENSMUSG00000039138 Vega Gene ID: OTTMUSG00000016215
Entrez Gene ID: 68175 CCDS ID:

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
4930591A17Riktm1a(KOMP)Wtsi MFCL C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
4930591A17Riktm1a(KOMP)Wtsi MFCL C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 4930591A17Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MFCL EPD0757_4_A12 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment
contact distributor 4930591A17Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MFCL EPD0757_4_A12 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/Harwell
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 29975)
order order view details

ES Cell Clones

Cassette Type 6 Knockout-First Alleles 3 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0757_4_A12 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a - Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_D10 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_E11 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_E12 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_G11 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_G12 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0757_4_B10 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_B11 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0757_4_E09 PG00049_Y_1_A04 C57BL/6N-Atm1Brd/a JM8A3.N1 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not confirmed LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
44417 PG00049_Y_1_A04 PCS00049_A_A04 L1L2_Bact_P R3R4_pBR_amp view
44417 PG00049_Y_1_A04 PCS00049_A_A04 L1L2_Bact_P R3R4_pBR_amp view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
18 4 0 2 view in Ensembl

4932414N04Rik

4932414N04Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 4932414N04 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms:
Location: Chr2:68656486-68748467(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1922971 Ensembl Gene ID: ENSMUSG00000079324 Vega Gene ID: OTTMUSG00000012911
Entrez Gene ID: 75721 CCDS ID:
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Rat LOC685215 685215

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
4932414N04Riktm1a(KOMP)Wtsi MCPJ C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
4932414N04Riktm1a(KOMP)Wtsi MCPJ C57BL/6NTac;C57BL/6NTac;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view pdf view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 4932414N04Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MCPJ EPD0083_2_C05 C57BL/6NTac;C57BL/6NTac;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR fail
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 36881)
order order view details

ES Cell Clones

Cassette Type 3 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0083_2_C05 PRPGS00052_A_A01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0083_2_D06 PRPGS00052_A_A01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0083_2_F05 PRPGS00052_A_A01 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
34526 PRPGS00052_A_A01 PCS00052_A_A01 L1L2_Bact_P L4L3_pD223_DTA_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
32 6 1 1 view in Ensembl

4933402N03Rik

4933402N03Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 4933402N03 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms:
Location: Chr7:131137713-131146314(-)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:1914681 Ensembl Gene ID: ENSMUSG00000013668 Vega Gene ID: OTTMUSG00000036750
Entrez Gene ID: 233918 CCDS ID:
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Human C10orf120 399814
Rat LOC499276 499276

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
4933402N03Riktm2a(KOMP)Wtsi MFAX C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
4933402N03Riktm2a(KOMP)Wtsi MFAX C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 4933402N03Riktm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MFAX EPD0611_3_A02 C57BL/6NTac;C57BL/6NTac;C57BL/6N-Atm1Brd/a view data
about )
WTSI/Harwell
Southern Blot na TV Backbone Assay pass 5' LR-PCR na
Loss of WT Allele (LOA) qPCR pass Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 30199)
order order view details

ES Cell Clones

Cassette Type 12 Knockout-First Alleles 11 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0611_3_A02 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA pass LOXP pass LACZ pass
Chromosome 1 pass Chromosome 8a pass Chromosome 8b -
Chromosome 11a pass Chromosome 11b pass Chromosome Y pass
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_A04 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_B04 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_C01 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_C02 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_C04 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_E02 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_E03 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_E04 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_G01 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_G02 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_H04 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0611_3_A01 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_C03 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0611_3_E01 PG00116_Y_4_H10 C57BL/6N-Atm1Brd/a JM8A3.N1 tm2e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_A01 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_A02 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_B01 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_B03 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_C03 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_D01 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_G02 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0301_5_H01 PRPGS00116_C_H10 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen pass LoxP Screen not confirmed 3' Screen not confirmed
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
113783 PRPGS00116_C_H10 PCS00116_C_H10 L1L2_Bact_P L3L4_pZero_DTA_kan view
113783 PG00116_Y_4_H10 PCS00116_A_H10 L1L2_Bact_P L3L4_pZero_DTA_kan view
113783 PG00116_Y_4_H10 PCS00116_A_H10 L1L2_Bact_P L3L4_pZero_DTA_kan view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
22 2 0 3 view in Ensembl

4933425L06Rik

4933425L06Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 4933425L06 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms:
Location: Chr13:105082122-105121782(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information

WTSI Phenotyping

MP Ontology Based Heatmap
Allele Name Colony Prefix Colony Strain adipose tissue behavior/neurological cardiovascular system cellular craniofacial digestive/alimentary embryogenesis endocrine/exocrine gland growth/size hearing/vestibular/ear hematopoietic system homeostasis/metabolism immune system integument limbs/digits/tail liver/biliary system mortality/aging muscle nervous system other pigmentation renal/urinary system reproductive system respiratory system skeleton taste/olfaction tumorigenesis vision/eye
4933425L06Riktm1a(KOMP)Wtsi MBJA C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view
Legend: No Raw Data No Significant Annotations Significant Annotation Present Link to a Test Report Page Link to a test report page
About This Heatmap

This heatmap is built up of top-level terms from the MP (Mammalian Phenotype) ontology. We have mapped a majority of the parameters in our tests to appropriate MP terms, so when we perform a given test we can say if it should be annotated with the given MP term (if the phenotype is significant). This is what you see in this heatmap - if a significant result has been annotated to a top-level MP term (or any of its children), the cell will be red, if the result was not significant, the cell will be blue.

Please Note: as mentioned above we do not annotate all of our phenotyping tests to the MP ontology. The tests that are excluded are: 'Adult LacZ Expression', 'Brain/Eye/Skin Histopathology', 'Embryo LacZ Expression', 'Fertility', 'Heart Histology', 'Micronuclei', 'MicroCT & Quantitative Faxitron', 'Plasma Immunoglobulins', 'Prepulse Inhibition', 'Recessive Lethal Study', 'Tail Epidermis Wholemount', 'Tissue Biobank' and 'Viability at Weaning'. You can find our results for these tests in the 'Phenotyping Test Based Heatmap' below.

Phenotyping Test Based Heatmap
Allele Name Colony Prefix Colony Strain Viability at weaning Recessive Lethal Study Fertility Embryo LacZ Expression Adult LacZ Expression General Observations Weight Curves Open Field Modified SHIRPA Grip Strength Hot Plate Dysmorphology Rotarod Non-Invasive Blood Pressure Prepulse Inhibition Indirect Calorimetry Glucose Tolerance (ip) Auditory Brainstem Response Body Composition (DEXA) X-ray Imaging Stress Induced Hyperthermia Eye Morphology Plasma Chemistry Plasma Immunoglobulins Haematology (CBC) Peripheral Blood Lymphocytes Micronuclei Tissue Biobank Heart Weight Heart Histology Tail Epidermis Wholemount Skin Histopathology Brain Histopathology Eye Histopathology MicroCT & Quantitative Faxitron Salmonella Challenge Citrobacter Challenge
4933425L06Riktm1a(KOMP)Wtsi MBJA C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view view view view view view view view view view view view view view view view view view view view view view view view view view view
download a spreadsheet containing all WTSI phenotyping data
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned Link to a Test Report Page Link to a test report page Link to a PDF Report Link to a pdf report

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 4933425L06Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) MBJA EPD0081_4_A11 C57BL/6Brd-Tyrc-Brd;C57BL/6Brd-Tyrc-Brd;C57BL/6N view data
about )
WTSI/KOMP Repo
Southern Blot na TV Backbone Assay pass 5' LR-PCR pass
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR pass Neo Count (qPCR) pass
LacZ SR-PCR pass 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR pass LoxP Confirmation pass 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 24592)
order order view details

ES Cell Clones

Cassette Type 3 Knockout-First Alleles 0 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0081_4_A11 PRPGS00031_A_A07 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0081_4_C11 PRPGS00031_A_A07 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0081_4_C12 PRPGS00031_A_A07 C57BL/6N JM8.F6 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential

No clones available.

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
41731 PRPGS00031_A_A07 PCS00031_A_A07 L1L2_Bact_P L4L3_pD223_DTA_spec view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
40 1 5 1 view in Ensembl

5830411N06Rik

5830411N06Rik was found for the following reasons:
ikmc_project_product_status_str - KOMP-CSD Mouse Available

Gene Details

Marker Name(s): RIKEN cDNA 5830411N06 gene
view this gene in MGI
Marker Type: protein coding gene
Synonyms: Scart2
Location: Chr7:140247301-140299791(+)
Related Human Conditions (from OMIM) - no related Human Condition
More Information
MGI Gene Accession: MGI:2443685 Ensembl Gene ID: ENSMUSG00000054672 Vega Gene ID:
Entrez Gene ID: 244234 CCDS ID:
Interpro Families
Orthologs / Homologs
Species Marker Symbol Entrez Gene ID Ensembl Gene ID
Rat LOC680907 680907

WTSI Phenotyping no data available

Mice

Allele Name Allele Type EMMA ID Colony Prefix ES Cell/Common Name Genetic Background QC/Screening Data MI/Distribution Centre
contact distributor 5830411N06Riktm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) BL3037 EPD0344_4_D12 C57BL/6NCrl;C57BL/6NCrl;C57BL/6N-Atm1Brd/a view data
about )
UCD/KOMP Repo
Southern Blot na TV Backbone Assay na 5' LR-PCR na
Loss of WT Allele (LOA) qPCR na Homozygous Loss of WT Allele (LOA) SR-PCR na Neo Count (qPCR) na
LacZ SR-PCR na 5' Cassette Integrity Neo SR-PCR na
Mutant Specific SR-PCR na LoxP Confirmation na 3' LR-PCR na
Genotyping Comment

IKMC Vectors and ES Cells

Vectors ES Cells
KOMP-CSD
(Project: 69369)
order order view details

ES Cell Clones

Cassette Type 4 Knockout-First Alleles 6 Targeted Non-Conditional Alleles
Promotor Driven
Allele: genbank file | image
Targeting Vector: genbank file | image
Allele: genbank file | image
Targeting Vector: genbank file | image
ES Cell Clones With Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0344_4_A11 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype 0.4 - 0.31 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR pass
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_C11 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_D12 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity pass
Distribution Centre
Karyotype 0.6 - 0.51 Copy Number pass Cells Thawed Correctly -
5' LR-PCR pass 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_E10 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1a(KOMP)Wtsi Knockout First, Reporter-tagged insertion with conditional potential (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen pass 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
ES Cell Clones Without Conditional Potential
ES Cell Clone Targeting Vector ES Cell Strain ES Cell Line Allele Allele Type QC/Screening Data
EPD0344_4_C10 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_C12 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_D09 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_E12 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_G10 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -
EPD0344_4_G11 PRPGS00101_C_G11 C57BL/6N-Atm1Brd/a JM8A1.N3 tm1e(KOMP)Wtsi Targeted Non-Conditional (Promotor Driven Cassette) view data    ( about )
Production Centre
5' Screen not attempted LoxP Screen not confirmed 3' Screen pass
Loss of WT Allele (LOA) - Vector Integrity -
Distribution Centre
Karyotype - Copy Number - Cells Thawed Correctly -
5' LR-PCR - 5' SR-PCR - 3' SR-PCR -
LOA - LOXP - LACZ -
Chromosome 1 - Chromosome 8a - Chromosome 8b -
Chromosome 11a - Chromosome 11b - Chromosome Y -
User/Mouse Clinic
Southern Blot - Map Test - Karyotype -
TV Backbone Assay - 5' LR-PCR - Loss of WT Allele (LOA) -
Neo Count (qPCR) - LacZ SR-PCR - 5' Cassette Integrity -
Neo SR-PCR - Mutant Specific SR-PCR - LoxP Confirmation -
3' LR-PCR -

Targeting Vectors

Design Targeting Vector Intermediate Vector Cassette Backbone Targeting Vector Genbank File
97014 PRPGS00101_C_G11 PCS00101_C_G11 L1L2_Bact_P L3L4_pZero_DTA_kan view
97014 PRPGS00101_C_G11 PCS00101_C_G11 L1L2_Bact_P L3L4_pZero_DTA_kan view

DNA Clone Resources

C57Bl/6J Bacs 129 Bacs MICER clones KO Targeting Vectors
14 7 0 2 view in Ensembl