WTSI Genome Editing (WGE) is a website that provides tools to aid with genome editing of human and mouse genomes
The CRISPR Finder will show CRISPR sites (paired or single) in and around genes. You can ask the finder to score the pairs for potential off-target sites, and browse individual and paired CRISPR sites using the Genoverse genome browser tool.
The Gibson Designer will find the oligos in either Human or Mouse genomes that can be used to create targeting vectors by Gibson assembly. The Gibson Designer matches the vector design with CRISPR sites appropriate for the creation of exon deletions.
If you use this site in your research, please cite:
Bin Shen, Wensheng Zhang, Jun Zhang, Jiankui Zhou, Jianying Wang, Li Chen, Lu Wang, Alex Hodgkins, Vivek Iyer, Xingxu Huang & William C Skarnes (2014) Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. doi:10.1038/nmeth.2857