High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by erb
Valid After Annotation
yes
Comment Type Comment Detail Edited User Edited Date
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 99236
Chr: 11
Strand: 1
Status: Ordered
BACs: RP24-70C22 : RP24-79L9 : RP24-435D21 : RP24-95B14 : RP24-178K5 : RP24-485B8 : RP24-409G4 : RP24-216E9 : RP24-340E15 : RP24-209N4 :
Instance: Plate 109: Well G04: BACs RP24-340E15 RP24-70C22 RP24-209N4 RP24-79L9
Oligos on GRCm38:
Type Start End Length Seq
G5 83742881 83742930 50 ATCCCATACGAATTCTCCTTTGGGGCAATCAACATTGTAACTGTAAACCA
U5 83748706 83748755 50 TTCTCACCACAAAGCAACAACAGCACCAGAAACACTTAGGGCTATAAAAC
U3 83748881 83748930 50 CTTTCCACAGATGCAATCCCTCTATCCATGTCTCTAAAGTAGGCTTTCTT
D5 83749548 83749597 50 AAAATCAAACTATATTTGAGCAAGAAGTTAAAAAATCACCCCCAAATGAT
D3 83749653 83749702 50 AAATCTTGTGCCTATTTGATTTCTTAACTATTTCGTTTGAAAAGACTTAG
G3 83752980 83753029 50 CCTCTCAGTTATCTAATCCTAATCGTGAAAAAGCTACTAATGTCAATACG
Oligos:
Type Start End Length Seq
D5_D3 83563100 83563154 55 GTCTGTTTATGACTGTGGTATATATTTAACTATGTACAAATATATATTAAAATGA
G5 83556383 83556432 50 ATCCCATACGAATTCTCCTTTGGGGCAATCAACATTGTAACTGTAAACCA
U5 83562208 83562257 50 TTCTCACCACAAAGCAACAACAGCACCAGAAACACTTAGGGCTATAAAAC
U3 83562383 83562432 50 CTTTCCACAGATGCAATCCCTCTATCCATGTCTCTAAAGTAGGCTTTCTT
D5 83563050 83563099 50 AAAATCAAACTATATTTGAGCAAGAAGTTAAAAAATCACCCCCAAATGAT
D3 83563155 83563204 50 AAATCTTGTGCCTATTTGATTTCTTAACTATTTCGTTTGAAAAGACTTAG
G3 83566482 83566531 50 CCTCTCAGTTATCTAATCCTAATCGTGAAAAAGCTACTAATGTCAATACG
G5_U5 83556383 83562257 5875 ATCCCATACGAATTCTCCTTTGGGGCAATCAACATTGTAACTGTAAACCACCTGCATGCCTGCTGGAGCATTAAACACAGGAGTGTCTGCAACAAGCTGCCCAAGAGCTCAGCCTGCAGTAAGGCCAGCTCTCAGAGAAGGAAGGTGAACACTCGCTCCCTTGGCTCATGCCTGCAGCGTGCTTCTACTCAAGTACCAGTCCTGCTGGCCTCTCGGTCTACACAAGAGGAAATTAAATTTCTTGAGGCACTAGACACTCTATTTAATTGGCAGCATAAAATAATCTGGCACAAGTTAAATCTGGCCAGGGCACGTGTGCACTGAGAGCTGAGAGCTGGTGGAGAACACGGTCACCCGTGCAAAATGCAAATGGAGTCACCCTTGGTAGTCCTGGGCAAGCAAGGTGACTTTTGGTTTCTACTTTTTATTTATGTAAACAAAGCACTATCTGTCTTAGTCGGGGTTTCTGTTGCTGTGATGAAACACCATGACCATAAATAACTTGGGGGGAGAAAGGGGGTTTATTTCAGCTTACAAATCTCAGGTCATACTCCATTGCTGAAGGAAGTTTTGCAGGGGTATGGAGGCAGGAGCTGATGGAGGAGCCATGGAGAGGTGCTGCTTACTGGCTTGTACCTCATGGCTTGCTCAGCCTGCTTTCCTACAGCACGTAGGACCACCAGCCCAGGCGTAGGACCACCCACCATAGGTTGGGCCTTCCCACACCAGTCACTAACTTTTAAAATGTCCTACTGACTTGTCCACAGGCCAATCTGGGTAGGGACATTTTCTCAACTGCTGTTTCTCAACTGAGGTCCCCTCTTCCAAAATGACTCAAGCCTGTGGCAAGTTGAGACTCTGGTTCTCAGCCAGCGGGATTTCTGTGGGCTCTTTGTTCCTCTCTAGCTTCCCCCTTTAAAGTGGAAGCTCTTTAAGAATAAGCCCCATACCTGTTGCCTCCATAACCTCTGCACTGAACACCAGCTCTGGCACACTCCAGCAGATGCATAAACTGGGTTCAAAATGAGCATGGTTCTTTATTGACTAAAATGAATTCTTCTCTACCTACAGTCACATGTTCAGTGGTACACGGTGGGATTCCAGTGGAGATTTATTGAGCTCCAATGCTGTCATCTTAAGGTGTGTACCTTTATTGATTGATTTATTGATTTATTGAGCTCCAATGCTGTCATCTTAAGGTGTGTACCTTTATTGAATACCCGGTCTAGCAGCTTCAAGAACTCAGTATGAGATCTAGGTAGGTCCACCCCAGCATCTTGTCCTCCCCTCAACCTCTCCCGTTCTCAATCTTCTCTCTCCCCCATCATCATGTGGCTAGAAAAATCTACACCATCACTCCCTAGAGTCACTGAGAACAATAAACACCCACATGGGGCATTACAAACAAACCCCTCCTCGCTATCAAATCCTCCCAGGAGAGAAATGTAAGGCAGCAGTAATTAAGTACTATGTGCTAACAGAGCTCATTATGGACTATGACAGTCTGAAGACCAAGTTACTCTCCCAGGGTTTGGGCCTGGAAAAGTTTCAGAGATGAAGTTTAAGCTAGGATGGCTGATAGAGGACTTGACTTCTGACAGACAAGAATGCCCACATTCACAGAAGAACGGAAAGGGAATTCCGTGTTCCAGGCAGAGCTTGAGCCAGACAGCTTAAAGGTTACTGTACTCAGCATCATGAATGAAGCAGAGCGGACGGGAGTGCCTGGATGGTGGAGACAAGTGGGGCCCAAGAGAAGACATGAGGGGGCAGGATGGAATGTTTCCCTCAAACTCACAAGTCCCCACTCATCTCTGAATATCTCTCAGGACCCAATAAAGCGCATGCTTAGGGCATCAAAAGTAAACTCAGAATTAAGGCTATCTGAAGAGAGATGAGAATGATCTCCATACACCTTTGACCTTTGTGTAACTTCAGAAATGGTTTTATTTCCAGTCTCAGTATTGTGAAAATATGGAATTACATGAAAACACACTGTGTACCCCCTTCAGTGCTTAAGATCTCTAAAAAGTCTTTATTAGTTGTTATGGTTCAAGCATAGCATGTCCCCACCATGGGCTTGGATGCTTGAATACTTGGTCCCTAGTAGGGGGTGCTGTCTGGGGAGGCTTCAGAAACTTGAGGAGCTAGAGCTTGTTTGAGGGAAGTAGGGTACTAGGAATGGGATGGAAGACTTAGGACCTGTTCTCTGTGTGTGCCTCTCCGTTTGTTTCTCTCTGTGTGTCTCTCTCCATCTGTCTGTCTCTCTGGTCTCTGTTTTTCTTCGGATCACTTCATGTCTCACTATCTCTGTCTCTCTTTCCCTCTTCTCGTCTGCAAAGCTGTGAGACGTCCTAGCTGCATAGATTGCTATGCCTTCCCCACCGTGATGACAACACCCTCAAATAGTGAACCGGGCGGAGCCAGAAAAAATCCTTCCTCCTTCCAGTTACATTAGCCAGGTATTTTATCACAGTGACGAGAAGTCAGTAATATACCAGATTTTGGACTATTGCAGAAATTGTACACCAGTGGGCTAGGCAGAGTGGAGTTAAAGCAGCTTGCAAAAATACTTTCTGCACGGCCCATAACTCTGTCTTCTAACCCCCTTGAGAGAGCTAGCAGTGTATGGCATCAGTTGTTTGTCAGAGGACCTCCAGCTTGTGGCATGTAAGATCTGTGACAAATGAAAGGAAGAGACTCTTCCTGTAGCTCCAGAATTAGGAACCATAGTCCTTGTGAAATGATAGTCCTCTCTTCCTCCCTCTCTCCCCTCGTGTGCGTGTGCGTGTGCGTGTGTGCGTGTGTGTTCCTAGGAATTAAACTTAGGGCCCCTGCCCTCTACTGTTGAAATAAACCATTTTCCCAGACACCTACAAAGGCACAACAAACTGATCCTGTGACTTCTCCCTCTCCCTCTTACCCCCTGCTTCCAGCTCATTTCATGTTAAGGTATTTTATGAGTTACAGTATTTGTCTTACTTAGGGTTTCTATGACTGCAATGAAACACCATGACCAGAAAGCCAGTTGGGGAGGAAAGGGGCTTTTTGGCTTACACTGCCAGATCATTGGAAGAAGTCAGGACAGGAACTCAAGCAGGGCTGGAACCTGGAGGCAAGAACCATGGAAGGAAACTGATTACTGGCTTGCTCAGTCTGCTTTCTTAGAGATGCTAGGACCACCACCAGCCCAGGGATGGCACCACCCACAATGGGCTGGGCCCTCTCCCATTGATCACTAATTGAGAAAATGCCTTAGAGCTGGATCTCATAGAGGCCTCTTCTTCACTGAGGCTCCTTTCTCTCTGGGAACTCTAGCTTGTATCAAATTGACACATTGTGTGTGCTCCTCCCCCACCACCAGGGAAAGAAGAGGGTATCTCCCCTGGCACCCAAACCACAGATGGTCTGAGTTACAAATCACTCATCTGGATTAGCAGGCGTGTCTACAAGAAGGAAGAAGTGAAAGCTCACACCTGGGCTAGTTGTCACTCTCACCCCACTCACTCTCCAAGTCGCACAGCAGTGGTGCAGCCTGAGAGGCATCCTGGGAGGGTTTGATGTAGATATTGACAGCTGGGGTTCCCCAAAATGAAGGCTCCAAACAGCAGAGATGATGGCATCAAGGCAGAGGGAACGACGCTGGCTTACTGTCTCCTCAACAGGTGGCCGCACCCCACTGGCTCTCCCACGGTCTCTCCCTGCCCTCAGTTACAGGAACAACTGTCCTAGAGCAAGGAGAGAGAAGTACAAAAGCAGATGGTGTCCTAGCTGACACCCAGCTCCTGGATTGTCATTCCCTGAGGACACATTCCTTATCACAGCTCATTCCTGGCCCAGAGTAGATCTCTAATACTTCATTGAAAAGGAACGAGAGTCCCTTACCACCCCAGCTTTCCAACTTTAGGAGGACTATCTGTGGAACAGAGGGTGTTCTGTGGCACGGAGCTTGGCTTGAGTAAGATGGCACTACCCAGGACATACATAGTGTGTGTCCCTGGCAATGCTGGTTTCCAGGCGGGGCTTTGGCTTCTGTGGTGTGCAGGCCCGCCCCTAGCCTTGCTGCCTAGCTCAGTGGGTGAAGAGGTTTAAATTCTGGCAGCTCTTGGCCCTGCTGCGTATTGGCTAACATCCTTCAGCAGCAGCATGAAGCTAGGAGCCTTCCTTCTGTTGGTGTCCCTCATCACCCTCAGCCTAGAGGTACAGGAGCTGCAGGCTGCAGTGAGACCTCTGCAGCTTTTAGGTACGTCTGGGCAAACACGGTTGCACTATTCTTCCTCAAGTGAGGGGTGAGAAGACATCCAGAACCAGTGTGTGCAGGAAGGAGGGAGAAACTGGAGGAAAGAGTTCTCTTTAGGATCCTCCTTGATGGTCACCTCTGAATTCCCCAGAACAGAATCCCTCTTCTGCTTTCCCTCTCTCCATTCCCTGGGTCACCTGACTGGACCCCCCTCCTGCTTGCTCCTCAGCCAAGCTCCCTCTCCCAGACGTCTCCTTCTCTACTGGCGTCTACCTTGACCTGTCTACAGAAAATATTCTTGTAATTATGCTAGACACAGGTCAATCTCAATCTTCTTTACCAATATTATTCTCAGAAAGGGTTTTGTTGCCGTGGGTTTTCATTGTTGTTTGTTTGTATGTTTGCCCAAGCTGGCCAGGAACTGACTGTGTAACCCAGGCTGACCTCAACAACATGGCAATCTTCCTGCTTCATCTTGCTATGCTGGGATTACAAGCACAATCCACCCCATCTTATTTGGCCTCAAAGGACTACTCTTAATCCTCTGCCCATTCTTCCCTTAGCCCTTTTGTGACCACAGCCTCATCAAACTCCTGACACACTCTCCTCTGTAATAAAGTCTCGCCTTTGCAGACTGATGCTGTCTGCCCACCCCTACCCCACAACCCCTATCCTACAGCCAGCCAGCCCTGGATTATCTTGGACTGAATGCTTGCCCCTAAATGTCTTTAGATAGAGTTTAGCCTCTCGTCTTCACTCCATCAACTACCTGTTTTTGAAACAGTTTGCATCTCTTCCGTCCTTTGCCCTCTGTTGCCTCACTGTGTCCATCCCTGATTACTCCACAGCCCTGCCATTGCAAAAGCTTCCCAGAAGCCCTCTCCACCCCCTCTTCCTTCTTGGATGTGCTTACTAGTTGGATCCTGGTTCTGTCTATGCCTTTGCAGCAGATAATCCTCCCAAATGACCTTCTCTCAGTTTATTAAGTGAAATCCCAAATCCACAGGTCCTGATGGCCTGCTTGCCCTTTGCAACATTTATTTCTCCTGTTCCCAAACTCGCCCAGACCTCTACTTGCCAATCCTCCTGACTCCTCCTATTCCAGGGGAACTGGGAGTGGCAGATAATATGTCTAAGGGGTAGGACTTGAAGAGTCAGTCTCTGGTCCTGGGAGTGCCTCTGTAGGTCCTGTGTCCCAGGTCACACACGGACCTCCCCAAAGTTCCACCCTACTACATTTCCCCTTGTGACTTTTTGCATCTTTCCACACAGCACCCCTCCTGCTTTCCCCCACTACTCTTTCTTGACAATATCCCTGTACTCCAAGGCTTACTTTTTCTGACGAACAACAAATAATGTTTTATCTCCTCTGAGCCCTTTGAGTCTTCTAGAACTACCTTGCAGGACACTTGTGAAACACTGCTCTGTCTCCAGTACCTTAGACATAAGTCTTACTTGCATTGGAAGGCTCTAAGAGGCCCTTTTTACATCCATGTCCTTCCTAAATGCTTCATATACAGCAAAATACCATAAATATTTAATGATTGCTCCAACGACTGTGATTAGGGACAAGAGATCTTCAGCTTTCTCACCACAAAGCAACAACAGCACCAGAAACACTTAGGGCTATAAAAC
U3_D5 83562383 83563099 717 CTTTCCACAGATGCAATCCCTCTATCCATGTCTCTAAAGTAGGCTTTCTTTAATGAGATTATGTGTGTGCCTTGTTTTAACAGAAATAGACAGTAACACTTTACAAATGGTATTATGACTTCAGTTAAAAGGAACTGGTGAAATGTACTCCTTAATCGAGGACTGTCGTCCTCAGCACTTGAATCTAAAGCATGGAGGTTTTCATTAGTCTATTTGTCTGTCCTTGAGAGGATAAACTCATCAAGTAATTCAGCTTGACCTTTAGCTGATGATCTGCCTGCCTTAGCCTAGGAGTCCAGGATTAAAGGTTTATTCCACCATGCCTGCTTTCAACTGATCTTTTCTGACATAGGCACCTGTGCTGAGCTCTGCCGTGGTGACTGGGACTGTGGGCCAGAGGAACAATGTGTCAGTATTGGATGCAGTCACATCTGTACTACAAACTAAAAACAGGTAAAAATAAAAATAAAAATTACTGTTCCTCCAAATCTTCCCCAGACCAGGCAAGTTGTACATTTGACTTCCATGGGAATTCTAGAAGTTTGGTAAAATGAAACCTATGTGTTTCCCTATATCTTCTCCTTTTTTCTTCATGAAGAAGCACCTACCAGAGGATGGTGCTTCCCAAACCTTTAGAATAATTTTCCAAACCAGTCTACCCAGGAGAAAAATCAAACTATATTTGAGCAAGAAGTTAAAAAATCACCCCCAAATGAT
D3_G3 83563155 83566531 3377 AAATCTTGTGCCTATTTGATTTCTTAACTATTTCGTTTGAAAAGACTTAGTGGACAATTTTGATCTCAGTACTTATCTTCAGCTCCATCTTTGCTTTATCTGTAGCATGTACAATGTATGCTCTTCTACCAGCAATCACTATAAATGTAAGCTTAAAATATTTATTTTATAATTAACATGACTGTATTTTGAATACTGAAATGTGCACACCCTTACTAATTCCTTTGGGATATATCCCAGCATGTATAATTGATGACCAAAGCATACTCTCATGTTGGCCCATATTAACCTAAGCAGATACTTTTTCTGTAAAAATATTTGATGGGATTCATATTATTGCATGTTTCCCTATTTAATTTTCAGTACCCACTAATTTTTTAAAAAAGAATAAAAAGCTACCATGATTAAGTTGTATTAATATGATTAAAATTGTATTAGCAGATTTAGCTTATACTCTAAACACCAAGCTGTCTGAGCCAACGAGATTGATCAGTGGGTAAAAGTTCCTGCTGCCAAGACTGGTGATCTGAGTTTGATCCTTAGGACCCACGTGGTAGAACTGACTCTCAAAAGCTGTCCTCTGTCCTCTACATGTGTGCTATGGCAGGCACATGACTGCACACACACACACACACACACACACACACACACACACACACACATTGAAACATACACACCTGAAACCACATTTTAAATTAAAAACAAAACCCAAAGAGTCAGGAGCCATCACAGGACAAGCTAATAGCTAGCAGGTGATCTTCCTGGAATGGTCTGCCTCGGAGTATAATAACCCATGAGAGATTTGCTCCGATTGGATTTGCATGGCCCAGGAGAAAATCACTTTAGGAAGTATTCCTGCTATTATGTTTTTCCTGTTATTATTAAATATATTTAACAATCCCTGGGTATTAGCCCATCCTTTTGAGCAGATCTCTGCAGATCTACAAAGATGTACTGCCTTATAGTGATGCTATATAGACAAATAGATGACTTAATTCTTTATGATGATCCTATAAGAATTACTAAAATTATTTACTAAGCTCTTTTATAGTGGGACTGCTATTAGATACTTTTCTGATAGTTAAAACTGCAATGAGAACTTTGTCAGTCTCCATTGTGTCACCAATGAATTGCTTCTAGATAGTAACCAGACTTTCTACTACTCAAAGCACATTCCAAAAGGTTGTAAAACCATTAACCAAAGGTCATAAAAAGGGAACTAATGATTTATTATAGATGCTAGGACAGAAGATAAAATATTGACTGGATTTATCTATACAAAACATCACTAATAACTTAGTTATGTTTTTTTAACTCTCAAAGAACTTGTGGAACTTTAACAGGTGATGAATCTTTAGTGGGATAATTACTCCTAATGGATATGCATGTAAACACTCTCTGTTGTAAACTTCAATTTATGATTTGAATTTGTGTAAACTTATGATGAACTTTTTACCATCTGATCATATATTCTGAAAGATATATTAGTGCTAAGGACAAAAAAGAAAACGAAGAACTGGGCTGGGGAGAACTTTTTAGACAGAACACTTTTTAGACAGATCTGAACAGAACTTTTTAGAATAGAACTGAGGATAATTCTTTTAGTCAGGACACAAGAGAACTTTTTAGACAGAATTGAACTCAAGAAGAACTTAGAAGTAAAGGCATATCATTGGGAGAAAAGGCATTGAGAGTAAGAGCATTGCTGTGACATCAGAGCAGGAAGAGAGAGCAAAATGAGGAGAATGCAGAGTGGAATAACTTAAAAACTATAGGTAACATAAAAATCTAAGAGAGCAATGTGCAGAATGCAGAGGGAAAGAGGGGAAAAAAGAAGCTGCATAGAGCAGAAGGAGCAGGCAGGCTTCTCCTTACCATGGAACAGAACAGGTCTTTTCTGAATAGCAAGGCAGGCTTATTCTTATAAAGGAACAAAGCTTTCTTCTTACAAACTTGGGTTTAACTCATTTAGCATTAAAAGGCTAGAAGCGTTTTCTTTCCCTATGCAATAAAGATTGGAGCTCTTTTTCCATGTAGAATCAGTGAGTTCTTTCTGAAACCTGTGCTTGGTCTTTTGCTCCAAATGCATATGTAAGTATAGACATGTGTGTGTAAGTATGTAATTGTGAGAATGTATGCAAGTTGGACACAACTGGTTTTAATGGAAGTGTATAAATGCACATATGTGATTCTGTGTATGAATTTACGTAAGTTTATGCATATTTGCTTATATAAAAGTTTTTCCTTCTGCAAGCATTATTCTCTTCTGGTTCAATAGAAGTTTATTGTTCAATAGCCTGACAAGCAAGAGGCATCTGAACAAAAGGGAAAAGGTTCTAGCAATTAATCCTTTACTTAATCTCCTGCTGTTTTAGGATGAGGTGCTAAGTGCCAGAGACTGCAGTTTCTGGCCTCAGGAACATAGAAGGCAGGTCAGCTACAGTAGCACAGTAACTTGGACTTACATAAGTGAACTTTTAATTATTATACTACAACCCTAAATATCTCACAAGACAAAAGTCGTACCCTCCGCCAGTGATCTTCCCCCCTCTGCTACCTCAAGGAGAACCAACAAGAAAGAAGACCCCACCGGGGCTGGCCCCCAGCACCAAAGTGTTCATGTTCAGAGTATTTATCTATCCAGCCCACCTGTAGTGCTTTCTGGCTTTCTGATGGTGCTATGGATTTCCAGCATCAAGATCTAATGTTTCTCATTGCACTCTGGGCAGTAGCCAGGTACCCAAAACAGCAGGTCTCGTGTATAATTTTTATATCTATTTCTCCAACAAAGAATTTTCTCAAAGGCCATCTTCCCTGATTACTGTTCATTTCTTCCTATAGCTTCTACCTGGAAAAAAAAATGTGTCTGTTTGGAGCTCTGTGACCAAGAAAACAGTTGAAAATGGAGGCCATGTATGGAGATTACAAGCAGCACAGTGGAGTGGGACAAGGAGTTGTTTCTTTTAATAAATCATTAATGTAAAAGTCTCAACACTTCTTGAGTTATTTTCTTGGAGGTTGTTATGGTTTGAATATGACACGTCTCCCTGGTCCATAGTTGATTGTACTGTTTGGGAAGAAGGGGCCATGCTGGAGGAGATAGGTCAACGGGGTATTTTGTCCTGGGCTTGACATGTTTCTTGTTGTTTCCTGGTTGCTTTAGGTGAGAAGCCTCAACCAAATACCCCTGCTGTCACGATCATTCTCCTTTACTACAAAGCAGCAGATTCAGTCAAGCATAGCCTTAAACCACCTAAAATAGTGCACCAAAAAATTAATAATAATAATAATAATAATAATAATAAATCCTCCTTCCTTTAAACTGTTCCTCTCAGTTATCTAATCCTAATCGTGAAAAAGCTACTAATGTCAATACG
U5_15 83547209 83562257 15049 AAAGATATGCCTATACACCCCTTTAATCCCAAACAATGAAGGTAAAGTTAGTTTTTACAAGGAAGCACCCATGTTTGAAAGTGACATCTAATTGACAAGCAGACAAAATGATAAATCATAGAAAGGTATTAAAGAATGAATCATGAGAACAGCACAGGAAATAGGGGCTACTTAAGAGAGCAGTGGGGGGAGGGGGGTCAAGGGAGAAGGAGGCATTTTTACCAGGACAGTTTTACAGAGACCGGTTGACAGGTTGCAGGTGAAGACAGAATGAGCTAGAGAAGGAGAAGGAGACAAAGGATTAGAACAAATTGCCAGAGTTAGTTTGAGGGCAGGCAAAGCAGTTCAGTGAGAAGCTGAGAGAAGCCAGATCGAATCAGTCAGCTTGGTCAGGAGTTTGAGCCAGAACAGCTGAGTTGAACGATGCTATTCAATTTGAGGGGCTTTTGAGATGGTGTGGAGAAGGAGACTTTTCCTTTTGGGACATCAGCTGTGGCAGAAGGTCAGCTGAGTGCTTCCTCTGCCTCTCTGAGCTAGCAGGCTTTCACCCCAAGATCTGGCTCCCAAGTCTTCATCAGAAAAAATAAGCAATTGAGATTTTGTTTTTAAAAAACACATTTTATTAAGTTACATACACTAGATATAGTGACATGAACTCCTACTTAAACATTACAGAACTACCACCTGGTGATTGGCCAAGACCAATGGTTCCCCAATCGTGTGGTTACTAAAAATGCAGCAGAAATCTAGCTGCATCCTTGTGATTTCCCCTGCCCTTCTCAAGGGAAAGATCTGTCTCAGAACCATCATTTTCAAGTATAAGAACCTTTGGATGTGGTTAACATTTGAATTTCTCAGTGAATACATATTTATCCATACTCATTCCAAATAATGTATTTGTAAATGTAGGCATGAAGACACACACACACACACACACACACACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGTGTGTTTTACTTTCCACCATACAATGTTTACGTTTTTCAAATATTTCCTTATTATTGGTATCTGGAATCAGTGCATGTTTTTATGAAATGTCAGAGAGTTCAGATGATAAGTTGTGTAAACCATAGGTTTCTTTGGCAACCACTCAGCCCTGCCATTGCAGGGAGAAGTCAGAGGATTTATAAATGAAACACTCTGATGGTGTTGTTCCAGTAACTCCCCCTCTTCTTCATTACCTGGAAGCTTTTCCCCTTTTTCTGGGATGAAAGCTGGCTCACACCCTGCATCCTTCCTGACATCTGCATCTTTCCTGACATCCTGCATCCTTCCTGATGACATCCTGCATCCTTCCTGTGCCTTTACTCAGCTAAATCTTATGCTTCCCAGATTTAAAAAACAAACAAAAACCCTTTCTTTCTCAATAAGTCCTGCTGGTGTCTAATTTAGCCAAGCCTAATCTGAAATGCCCTGCTGAGCCCTGGTCCCTTTGTTTCCAGGCTATAGCACACCACCACCATCAGCAGCTGCCACTGAATAGGAAAGCCACAGAATAGCTATAAGTCTGGAAAGATAAGAACAAAAAAACCCACTGTACTTAAACCCTAGAATATTCTCCCCATGGTGTCATGTGTGCTGTGAATGTGTTACCAAACTGGGCTCACTTTGCCCTAACCACACAAACCATTGAAATAAAGGGATTTACAAAAGAGAAATGAGGAGATGGCCAAGAATTGAGGCAGGAGAGTAGATCTCCCCTCCCCACAGAAAGAGTTTAGAAGTGGTTGAGGTGTTGGGAAAGACACTAAGTTCTTGGACCCTAATAGGAGCTTTTATCATCCAACTCCATCCATCATGACCCCACATTGAGTGTCCTCTGCCCCTCTAAAATAGCAACTGTCCGCATGGGAGCTCGGATGCAAGGAAAGCTGTGACTTCCCTCTCTTCTCAGAAGTGATATTGCTGTCACCTGGATGGAAGCAATGAATCAGAAATTGAGAAGCAGATTAGACTTTTAGCCCTGGCGCATTGTGTGTTTGATATGTTCTGGCTTCTACGTGGTCATCTCTTTAACTGTGTAAAGGAAAATCACAGATACCTTAGGAAGGCAGCACCCCAGCACTGCGGAGCCATGACAAGGAGATCCTCTCTTTTGCCTCACACTTTCTCCTTCTCCATGGTCAGGAAAGTGGAATTGTAGATGACAAGTCCACTCCTCAAGCATCTTGTCAGTCTAAAAATAAAAAGTATGTGTAAGGTGAAGCCATCATTTAGCCATGAGCATTAACTCAGAAAACCTGGGCCTCATCCCCCTAGACACAGGAGCCTCATAGCTAGGCCATGGCGGGTGGGTGTGGCAGGACCACGTGTGTGCACTCTGGTTTCCCAACACCATGAGGATGCCAGTGTCTCTCTCTACTGATAGACACAGTGCTCACTTCTCCTGCATGCTATCAGAATAATACTTCCTGTGCTTGCTCTCTGATGCTGGAACATAAAAACATTGACCTACTCTGCACCCCCAAAAGACTGGTTGGCAGCTCCTCTTTAATTTGGCTCTCCCTCCTGTTCCCATTCTCCCCAGCAACTTTTTGTTGAGGTTGGTCTTGTGCACTGTGTATTCAAATGCTGAGTTCTGTGCCCCACGATGGGGTTGCAGTCTGGATATGGTCATTCTATTCACCAATGTGCTGTAAAGAATGCTCCTGAATTATCTCTGATTTGATAATAAAAGGCTAGAGCCTGTGACTGGGCAGGAGAGAGAGAAAAGTGGGACTTGAGATTGAGTAGGGGGGGGTCACAGGTAGGGACCGCGAGAGAAGGAAAACGGTAGGCTAAGAAGGCGGTTGTCATGAGGCAGGATAGACCATGAGGAAGAACCATTAGCAACTGGCCAAAAGAAACTCACATTGAAGACAAATATGGAACAGATCAAGGCTCAGCTGGCAAGTAATCCAGCATTTATCTGGTTATTAATAGCCTATTAGGGTTATAATAGTCTCAGGACCTGCCCAGTTTAGGTTGTTAAAAAATTCTGATGTCTTTGTGTTGTTTACTTGGGAGCTAACTGGGGAAAGAGTGTGGCAGAAACCACACCCCTATACAATTACTTTAGTACCTTTCCCCCTCCCTTCTCTGAACAGATGCACCACAACCATAGCTGTTCTATAAACTACAATCCCCAATTGTCACTCTCTGCTGTCCACGGGAAATGTTTCAGGTTTTCTCTCCTGCCCCACGTAGTTTCTTCATATCATTCATGCTATCAAATTGATGCAAAGTCCTTATCTTTTTCTTTTCTCTGTTTTTTTTTCTTTTTGGTAGGGCCTCCATAACTATCTGATTGGATAATGAAAGGCTTAAACTTGATAGTTGATTCTCTAAGTATAAGTAACAGAAGCAGACAAATATTAAAGCGAGGGAAGCCATTTCTTCAAGAGGACACATGTGTGTCACAAGGACCACTCCTTGCTCCCTGCCCCTTGTCTCTGTTTCTCAAATACATTACCTAGCACCCACTGCCAAGAAGAGACTCCGGCATTTCTACTATTGCAACAACATAAGGACAGAGGCCTTGACCCAGCATATTAGATGTCTGCCCTTGACTGGGGTTTAGAGCTGTAGTGACACAAAGCAGTTTTTGCCTTTAGTTGGCCCTTGGTAATGAATCGATAGGTACAAAACAAAGTGATGAAATCATGGAAATGCAAATCAAAACCACAGGAGACACCATCTCACCCCAGTCAGAGCAACTTTTATAGAAAGCCCCAAAATAGCAAAACTAGTGAGGCTGTGGAGAAAGGGGAACTCCCATTTGGGCACTCTCGGTAGGAATGTAAATTAGTACAGCTATTACAGAAAACAGCACAAGAGTTCCTCAAAACCATGAAACAAGACTGCTATCAGATTCAGCAATCCCACTGCTGGGCATTGAAATGAGATGAAATCATTATATCAAAAACATATCTGCAGTCCCTGTTACAGCATTATTCACAATGGCCGGGATGTGGAATCAACCAAGGTATCCACTGGTGGATAAACACACAACTACATATTGGTGGTAGGACTGTGTGTAGTCATGAAAAGACTGATGTACTATTATCTGCAATGACATGGCCAAAGCCAAAAGACACTGTGTTAATCGAAGTAAGCCAGGCTTATGCAGACAAATGCTGTGTGTTCTCATTCACTATAGGAACAAAATGTTGATAACATGAAAGTAGAGAGCAGAACAGAGGTTGTCAGGAGCTATCTAGGAATACTATAGGGGAGGAAGGGTTTGGACAATTAGCACCAAAACACAGTAGAAGACTGAATCCTTGGTCTCACAGCACACTACCTCACTCTGAGGTCCACACCAATTTCTTATCTGTAACTATAAGACTATTAAAGAGTGAAGGTTCCCAAAACAGGGTGATCAGTGTTTGACACGCATGGTGATGGTCCTGGTCTGATCACTACATGTATATATGAATGGAACTATCACACAGTGCACACAAGTATGTACACATGATACATGCTAATCTATAACTTTCAATTTTATGCAAGAGTTAAAGTGTTACAAAACTATAGGTCAAAAGATCAGCTCTGTCTGTAAGTTTATGTTTTTGAAATAAATACCATTAGTAGTTTGGTAGGCATTATTCCCATTTATATTCTATGGTATGTCAGTAACTGTATTATTTTATATATTCACTACAGTCTATAATAATACTGTCTGGGAGCAGACCCATTTTCTCACTGCCAATCATCCTTCCAGGGACATATCTCAGAGGTCTGTCTGTGTTCATGGTGTCATTTCTGTGCAGATTGCTGCCAAAGGACCTGGATCTGTGGAATCATATATCAAGAAGGACATGGTCATGTCTGCTCTGGTCATGTCTGTTCCCCCAGCCTGGGAACCAGGTGGAAGCACCAAGCCCTGCCCAGGTCCCCACCCCACACAGCCCAGTGAGCGCATGCCTAGCTCTGCCCTGGCTTTCCTTACTTCCATGGTTAGTGCTCAGGCTCTGTGCTCTCAAGTCTGTGAAGAGTAGATCACAGACACTTTCCCAGCCTTTCTTGAGCACAGAACACTCTGCTCTTGCCTTATTATACAAGTCAGATGTATGTAAAGTGGGAAAATTCAGAGACGGTATTAACCTGTCTTCTCACATAGAAAGCCACATTGACATTCTAAATGAAAGCTTCTACACACATGGACACACATAGACATACACAAACATACATACATACATACATACATACATACACAGACAGACAGACAGACAGACATACAAACACAGACATACACGTGCATACATACACAGACACATAAACATACATACACACACATAGACATAGACATACATACATAAACATACATACACATACACACACATACATACATAAACATACATACACACACACACACACACACACACACACCACACACCCTATTTTGTGTATCCATGGATAGACATAGCCCAACCTGATTGATGTCAAAACCAACTCCATATCAGTAGCCCTATCCTGACACTGGATGCAAAGAGAAAGAAGGCAGCCTTGGTGTCTGAGAATCAGGAAATACATCGTTCCTCCGTGGCCAGAGGACCATGAAGACACAGATGGATAATATCTTGCTTAGAAGGCACTATAAAGTTGAAAAAGCTGGAAATACTCTTAGAAATTACAGTGTTGTAAGGAAGTTAGCCCAAACTATGCTCCCCAAACCTTATGTGAAACATAACATCCCAGTGTTCATGCTGCCACCTGGTGGATGTGACTGTCGCTGCACCTCTGTACCCCTTGCTTCTTTACTAAAGCCTATAGTTGATATTTCCACTATAAATCTAGGAAGACTGTCAAAATGACCTGAGACCAGAGGAGCTGAGACTGAATGTAATCCAAGAACATCTTGAAAGCACATATTCATTCTCTCAGGATTACGGGCCAAACTTAGATACGCAGGCAATGGGAAGAGAATGCTGAAGAACCCTGCTGATAGAGTTATCTCACAGCGCCAAGTAGATCCACTCAATGTATTCACTTAACATGTGAGTGTAGAATCCAGTACACATCTTCCACGATGCTAACTTGAGAGAGTAAGATGGTTTTTAATGGGTTGCTGTGCAGCAAGAGTATGCTGCTGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTCTCATTAAACTTTAGATTACTGCTTCAGTAGGTCTGGCGTGGAATCCAAGAGTCTTCATTTCTTCCAAGGGATCTGTGCAATGGACAGACGCTGGTGACCTGTAAGTCTGCACTTATTTCAGGCTAAGTGCAAAGCATTTGTCAACCTTACCCTTCTCTCTGAAAATCACTGTCTCATTCTTCTGCTGAAATCCCGGAGAGTCACAAATATTCCCTCTCTTGTGTGACAAGATTAGATAGATAGATAGATAGATAGATAGATAGATATAAAACTCCAAATCACCCCCCATCTTACACACCACCCCAAACACCTGCCACCTCACTAGGCTAAAAGTGTGAGGGGTTTTGACAAATGTCCACACAAAGACCCATGTGATAAAGGCACTGTCTTCAGTCTCTTCTCTGGCACCATTCAGAGATGGTGGGATATCTTGGAGTGCAGTATAGTTGGAGGAGGTTTTGGGAGGCATGAGCTTGAAGCAGATATGGGGACCATTCAGTGCTCCTCTCTCTTTTTCTACCTGCTGCTTGCCCTGAGGTAGGTAAACTTTTCTCTGCCCCATAGTCCAATCATGATGCACAGTTTTGCTTCAGGCCTGAGAAGGAATGGAGCTGACTAACTATGGACAGAAACTTGTGAAATCCGGAGCCAGAACCAACCTTGCCTCTTTGTAAGTTGTTTACCTTAGAGATGTAATAACTTCATAGGAAATTATCTAACAGGTGGCGCAAAGTTTTGACAGAATTACAAACTGGATTGGTGCTATTTAAATGTAGTAAGAATGTGGTTGGTGTTCCTCAGTGGCTGCCCTACCACTGTTACTCAAGAATAAAATCTCCAAGCCACCTGTTCCCCGCAAAAGTGCACCTCAAGTAGTTTAACCAAGAAAGGAATGTTAAGTAGATTGAACTGTGAAACAGAGGAAGCCAGTCCCTATTCCTAGACTGTGCTCTGAGATTTGGATGCCAGGTGAATACAAATGGGAGCTACGCAGATACACAGGTGAGGTGTGGACAGAGTTCACAGAGGGAAGGTGTGGAGGAGAGAGTTTGGAGAGAGGTGCTGAATCTTGGCTAAGGGAGTGACTCAGTGGGTAAAAGCTCTGGCTGTACAGCTATAAGAGTATGAGTTCAAGTCCCTCGTCCACACTAAAATGCAAGCCATCCTTCTGCCTAGACATTTAGAGCACTACATATGGGATGGAGACAGGAGAATCATGGGGGTTGGCTGGCTGTCAGCATAGCTCCACTTCAGAGAGAGACTCTGCCTCAAGGGTAGAGAGTCATAGAGTGGGACACCTGATGACACGTTTACAAGAACACATAGGTGTGTGCACTGGCATATACACACAGGTATACACCAACACACACACACAGCTCACAAACATGAGGAGGAGGAGGCAGCAGCAGCTGTGTGTAGTTGGGCTAGGTATGGCCATGTTTGAGATGGAGCTAGATATTGAGAATTTGTGTAATAACAGGAGTAGAGATGGGAGATACACATGTATGGTGTGTGTGTGCATGCATGTGTGCATGTGTGTGTGCGCATGTGTGTGTGCACAAATGTGCTGTGCTGTGTGTGTGTGTGTATGTAATTCAGCACATCCAACTTCCCAATGTGTACTGATTTCCTTTCTCCCCACCCCATTTCTATCTGTCACTCAAAGTGGCCTACATTCTTTAATAGTCTCAATTATGAAGGAGAAGGGCTACTTTCCTTCATTTGGTATGCTGGTATAAAAGTATGTCATTACTCAATGCTAAGAAAAGATCTCTCATTCAGAAACCTCAGATACCTATCAGGGCTAGGCTCTATTTGCCTGTCTTGACCCAGGTTGCACATGCCAGCCTGGCTCAGTCGGAGGACATGTGTCATCTTTTCTCTGTCTTGACCCATCCATGCTATAACTAAGGAGACTTCATCAGCAACAAGATGCTGTAGTTTTAAGACCATGTCCTCAGTTGCACCAGTGTCTGCTTGGCTGGGGTCAAGTACTCAGGTCAAGATCCAGCTCCGGCGTTCAACAGAATCTTAGCAGTTGCACTATCTAGATCTGGTTCAGATGAAGGAAAGTGACAGGTGGGTGGACACATGCCTCTCATCCTCCTGGGGGTGGACCTTAACCCAACGGCCCCGCCAGGTCTCAACGTTCTAGTTTCAGAGACCAAGGGACAGGACATTTGGAACACTCTGGTTCTCACTAATCAATGTTGTCAACAATCCCTCTACCTTGCAAACATTCTTATCAAGAATGGGAGCCCTTTGGGTGGTGACAGTTCCACCACACACACATCCCCTGACAGTGTTGTGCTCTGTATTGCCTGTAACTTTTTTCTTTGATCATGCCCAAACTAATGACTTTTTATCAGAAACTGCTCAGATGCTATGCTTCTGACCTCGGTATGGGAACCATGAACCCATGTCTGATCACTGAGCTAACAGGAATGCCTTTTGTAAACCTTCACAGTACTGAATGCTGATGGTTTAAAGGGTGCTGTACCAAGAGTATGAATACATACATACTGTACTATCTTTTTCATCTCATATACCCAATACAGGAAGACCCCTGTCAGTGCAGCAGGGCTAGCAGCCCAGAACTGGCATTGGCAACCTAAGGAATTCTTCCATGTAGGGGTGTTTACTCAGGTAAAGCCAGCTCTTTGACTGAACCACAGAAAAGAACTTCAGGGTGAGTCAGAAGAGAGCATATTTAGACAAAGAGATGGACAGAAAGGGATAGTACAGGACAGAGACATGAGTGCAAATCAATCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCCCTCCCTCTCTCTCTCTCTCATTTTTTGAACACAGTGTTTCTCTGTGTAGCTCACAGGCTTCGCCTGTACTATTATGTCTGGCTGACACCACAGGGCTCAAATTCTCCCCTTTGGTATCCTGGTGATCACTCTCTCCCTCACATGGTTCAAGACAGAGGATGGGGGTCAGCGGAGAGTGAAAGGCAGAGCTGAAGTGGCAGTGAAGCAGAGATGGCTCTGCCACATCCCATACGAATTCTCCTTTGGGGCAATCAACATTGTAACTGTAAACCACCTGCATGCCTGCTGGAGCATTAAACACAGGAGTGTCTGCAACAAGCTGCCCAAGAGCTCAGCCTGCAGTAAGGCCAGCTCTCAGAGAAGGAAGGTGAACACTCGCTCCCTTGGCTCATGCCTGCAGCGTGCTTCTACTCAAGTACCAGTCCTGCTGGCCTCTCGGTCTACACAAGAGGAAATTAAATTTCTTGAGGCACTAGACACTCTATTTAATTGGCAGCATAAAATAATCTGGCACAAGTTAAATCTGGCCAGGGCACGTGTGCACTGAGAGCTGAGAGCTGGTGGAGAACACGGTCACCCGTGCAAAATGCAAATGGAGTCACCCTTGGTAGTCCTGGGCAAGCAAGGTGACTTTTGGTTTCTACTTTTTATTTATGTAAACAAAGCACTATCTGTCTTAGTCGGGGTTTCTGTTGCTGTGATGAAACACCATGACCATAAATAACTTGGGGGGAGAAAGGGGGTTTATTTCAGCTTACAAATCTCAGGTCATACTCCATTGCTGAAGGAAGTTTTGCAGGGGTATGGAGGCAGGAGCTGATGGAGGAGCCATGGAGAGGTGCTGCTTACTGGCTTGTACCTCATGGCTTGCTCAGCCTGCTTTCCTACAGCACGTAGGACCACCAGCCCAGGCGTAGGACCACCCACCATAGGTTGGGCCTTCCCACACCAGTCACTAACTTTTAAAATGTCCTACTGACTTGTCCACAGGCCAATCTGGGTAGGGACATTTTCTCAACTGCTGTTTCTCAACTGAGGTCCCCTCTTCCAAAATGACTCAAGCCTGTGGCAAGTTGAGACTCTGGTTCTCAGCCAGCGGGATTTCTGTGGGCTCTTTGTTCCTCTCTAGCTTCCCCCTTTAAAGTGGAAGCTCTTTAAGAATAAGCCCCATACCTGTTGCCTCCATAACCTCTGCACTGAACACCAGCTCTGGCACACTCCAGCAGATGCATAAACTGGGTTCAAAATGAGCATGGTTCTTTATTGACTAAAATGAATTCTTCTCTACCTACAGTCACATGTTCAGTGGTACACGGTGGGATTCCAGTGGAGATTTATTGAGCTCCAATGCTGTCATCTTAAGGTGTGTACCTTTATTGATTGATTTATTGATTTATTGAGCTCCAATGCTGTCATCTTAAGGTGTGTACCTTTATTGAATACCCGGTCTAGCAGCTTCAAGAACTCAGTATGAGATCTAGGTAGGTCCACCCCAGCATCTTGTCCTCCCCTCAACCTCTCCCGTTCTCAATCTTCTCTCTCCCCCATCATCATGTGGCTAGAAAAATCTACACCATCACTCCCTAGAGTCACTGAGAACAATAAACACCCACATGGGGCATTACAAACAAACCCCTCCTCGCTATCAAATCCTCCCAGGAGAGAAATGTAAGGCAGCAGTAATTAAGTACTATGTGCTAACAGAGCTCATTATGGACTATGACAGTCTGAAGACCAAGTTACTCTCCCAGGGTTTGGGCCTGGAAAAGTTTCAGAGATGAAGTTTAAGCTAGGATGGCTGATAGAGGACTTGACTTCTGACAGACAAGAATGCCCACATTCACAGAAGAACGGAAAGGGAATTCCGTGTTCCAGGCAGAGCTTGAGCCAGACAGCTTAAAGGTTACTGTACTCAGCATCATGAATGAAGCAGAGCGGACGGGAGTGCCTGGATGGTGGAGACAAGTGGGGCCCAAGAGAAGACATGAGGGGGCAGGATGGAATGTTTCCCTCAAACTCACAAGTCCCCACTCATCTCTGAATATCTCTCAGGACCCAATAAAGCGCATGCTTAGGGCATCAAAAGTAAACTCAGAATTAAGGCTATCTGAAGAGAGATGAGAATGATCTCCATACACCTTTGACCTTTGTGTAACTTCAGAAATGGTTTTATTTCCAGTCTCAGTATTGTGAAAATATGGAATTACATGAAAACACACTGTGTACCCCCTTCAGTGCTTAAGATCTCTAAAAAGTCTTTATTAGTTGTTATGGTTCAAGCATAGCATGTCCCCACCATGGGCTTGGATGCTTGAATACTTGGTCCCTAGTAGGGGGTGCTGTCTGGGGAGGCTTCAGAAACTTGAGGAGCTAGAGCTTGTTTGAGGGAAGTAGGGTACTAGGAATGGGATGGAAGACTTAGGACCTGTTCTCTGTGTGTGCCTCTCCGTTTGTTTCTCTCTGTGTGTCTCTCTCCATCTGTCTGTCTCTCTGGTCTCTGTTTTTCTTCGGATCACTTCATGTCTCACTATCTCTGTCTCTCTTTCCCTCTTCTCGTCTGCAAAGCTGTGAGACGTCCTAGCTGCATAGATTGCTATGCCTTCCCCACCGTGATGACAACACCCTCAAATAGTGAACCGGGCGGAGCCAGAAAAAATCCTTCCTCCTTCCAGTTACATTAGCCAGGTATTTTATCACAGTGACGAGAAGTCAGTAATATACCAGATTTTGGACTATTGCAGAAATTGTACACCAGTGGGCTAGGCAGAGTGGAGTTAAAGCAGCTTGCAAAAATACTTTCTGCACGGCCCATAACTCTGTCTTCTAACCCCCTTGAGAGAGCTAGCAGTGTATGGCATCAGTTGTTTGTCAGAGGACCTCCAGCTTGTGGCATGTAAGATCTGTGACAAATGAAAGGAAGAGACTCTTCCTGTAGCTCCAGAATTAGGAACCATAGTCCTTGTGAAATGATAGTCCTCTCTTCCTCCCTCTCTCCCCTCGTGTGCGTGTGCGTGTGCGTGTGTGCGTGTGTGTTCCTAGGAATTAAACTTAGGGCCCCTGCCCTCTACTGTTGAAATAAACCATTTTCCCAGACACCTACAAAGGCACAACAAACTGATCCTGTGACTTCTCCCTCTCCCTCTTACCCCCTGCTTCCAGCTCATTTCATGTTAAGGTATTTTATGAGTTACAGTATTTGTCTTACTTAGGGTTTCTATGACTGCAATGAAACACCATGACCAGAAAGCCAGTTGGGGAGGAAAGGGGCTTTTTGGCTTACACTGCCAGATCATTGGAAGAAGTCAGGACAGGAACTCAAGCAGGGCTGGAACCTGGAGGCAAGAACCATGGAAGGAAACTGATTACTGGCTTGCTCAGTCTGCTTTCTTAGAGATGCTAGGACCACCACCAGCCCAGGGATGGCACCACCCACAATGGGCTGGGCCCTCTCCCATTGATCACTAATTGAGAAAATGCCTTAGAGCTGGATCTCATAGAGGCCTCTTCTTCACTGAGGCTCCTTTCTCTCTGGGAACTCTAGCTTGTATCAAATTGACACATTGTGTGTGCTCCTCCCCCACCACCAGGGAAAGAAGAGGGTATCTCCCCTGGCACCCAAACCACAGATGGTCTGAGTTACAAATCACTCATCTGGATTAGCAGGCGTGTCTACAAGAAGGAAGAAGTGAAAGCTCACACCTGGGCTAGTTGTCACTCTCACCCCACTCACTCTCCAAGTCGCACAGCAGTGGTGCAGCCTGAGAGGCATCCTGGGAGGGTTTGATGTAGATATTGACAGCTGGGGTTCCCCAAAATGAAGGCTCCAAACAGCAGAGATGATGGCATCAAGGCAGAGGGAACGACGCTGGCTTACTGTCTCCTCAACAGGTGGCCGCACCCCACTGGCTCTCCCACGGTCTCTCCCTGCCCTCAGTTACAGGAACAACTGTCCTAGAGCAAGGAGAGAGAAGTACAAAAGCAGATGGTGTCCTAGCTGACACCCAGCTCCTGGATTGTCATTCCCTGAGGACACATTCCTTATCACAGCTCATTCCTGGCCCAGAGTAGATCTCTAATACTTCATTGAAAAGGAACGAGAGTCCCTTACCACCCCAGCTTTCCAACTTTAGGAGGACTATCTGTGGAACAGAGGGTGTTCTGTGGCACGGAGCTTGGCTTGAGTAAGATGGCACTACCCAGGACATACATAGTGTGTGTCCCTGGCAATGCTGGTTTCCAGGCGGGGCTTTGGCTTCTGTGGTGTGCAGGCCCGCCCCTAGCCTTGCTGCCTAGCTCAGTGGGTGAAGAGGTTTAAATTCTGGCAGCTCTTGGCCCTGCTGCGTATTGGCTAACATCCTTCAGCAGCAGCATGAAGCTAGGAGCCTTCCTTCTGTTGGTGTCCCTCATCACCCTCAGCCTAGAGGTACAGGAGCTGCAGGCTGCAGTGAGACCTCTGCAGCTTTTAGGTACGTCTGGGCAAACACGGTTGCACTATTCTTCCTCAAGTGAGGGGTGAGAAGACATCCAGAACCAGTGTGTGCAGGAAGGAGGGAGAAACTGGAGGAAAGAGTTCTCTTTAGGATCCTCCTTGATGGTCACCTCTGAATTCCCCAGAACAGAATCCCTCTTCTGCTTTCCCTCTCTCCATTCCCTGGGTCACCTGACTGGACCCCCCTCCTGCTTGCTCCTCAGCCAAGCTCCCTCTCCCAGACGTCTCCTTCTCTACTGGCGTCTACCTTGACCTGTCTACAGAAAATATTCTTGTAATTATGCTAGACACAGGTCAATCTCAATCTTCTTTACCAATATTATTCTCAGAAAGGGTTTTGTTGCCGTGGGTTTTCATTGTTGTTTGTTTGTATGTTTGCCCAAGCTGGCCAGGAACTGACTGTGTAACCCAGGCTGACCTCAACAACATGGCAATCTTCCTGCTTCATCTTGCTATGCTGGGATTACAAGCACAATCCACCCCATCTTATTTGGCCTCAAAGGACTACTCTTAATCCTCTGCCCATTCTTCCCTTAGCCCTTTTGTGACCACAGCCTCATCAAACTCCTGACACACTCTCCTCTGTAATAAAGTCTCGCCTTTGCAGACTGATGCTGTCTGCCCACCCCTACCCCACAACCCCTATCCTACAGCCAGCCAGCCCTGGATTATCTTGGACTGAATGCTTGCCCCTAAATGTCTTTAGATAGAGTTTAGCCTCTCGTCTTCACTCCATCAACTACCTGTTTTTGAAACAGTTTGCATCTCTTCCGTCCTTTGCCCTCTGTTGCCTCACTGTGTCCATCCCTGATTACTCCACAGCCCTGCCATTGCAAAAGCTTCCCAGAAGCCCTCTCCACCCCCTCTTCCTTCTTGGATGTGCTTACTAGTTGGATCCTGGTTCTGTCTATGCCTTTGCAGCAGATAATCCTCCCAAATGACCTTCTCTCAGTTTATTAAGTGAAATCCCAAATCCACAGGTCCTGATGGCCTGCTTGCCCTTTGCAACATTTATTTCTCCTGTTCCCAAACTCGCCCAGACCTCTACTTGCCAATCCTCCTGACTCCTCCTATTCCAGGGGAACTGGGAGTGGCAGATAATATGTCTAAGGGGTAGGACTTGAAGAGTCAGTCTCTGGTCCTGGGAGTGCCTCTGTAGGTCCTGTGTCCCAGGTCACACACGGACCTCCCCAAAGTTCCACCCTACTACATTTCCCCTTGTGACTTTTTGCATCTTTCCACACAGCACCCCTCCTGCTTTCCCCCACTACTCTTTCTTGACAATATCCCTGTACTCCAAGGCTTACTTTTTCTGACGAACAACAAATAATGTTTTATCTCCTCTGAGCCCTTTGAGTCTTCTAGAACTACCTTGCAGGACACTTGTGAAACACTGCTCTGTCTCCAGTACCTTAGACATAAGTCTTACTTGCATTGGAAGGCTCTAAGAGGCCCTTTTTACATCCATGTCCTTCCTAAATGCTTCATATACAGCAAAATACCATAAATATTTAATGATTGCTCCAACGACTGTGATTAGGGACAAGAGATCTTCAGCTTTCTCACCACAAAGCAACAACAGCACCAGAAACACTTAGGGCTATAAAAC
D3_15 83563155 83578205 15051 AAATCTTGTGCCTATTTGATTTCTTAACTATTTCGTTTGAAAAGACTTAGTGGACAATTTTGATCTCAGTACTTATCTTCAGCTCCATCTTTGCTTTATCTGTAGCATGTACAATGTATGCTCTTCTACCAGCAATCACTATAAATGTAAGCTTAAAATATTTATTTTATAATTAACATGACTGTATTTTGAATACTGAAATGTGCACACCCTTACTAATTCCTTTGGGATATATCCCAGCATGTATAATTGATGACCAAAGCATACTCTCATGTTGGCCCATATTAACCTAAGCAGATACTTTTTCTGTAAAAATATTTGATGGGATTCATATTATTGCATGTTTCCCTATTTAATTTTCAGTACCCACTAATTTTTTAAAAAAGAATAAAAAGCTACCATGATTAAGTTGTATTAATATGATTAAAATTGTATTAGCAGATTTAGCTTATACTCTAAACACCAAGCTGTCTGAGCCAACGAGATTGATCAGTGGGTAAAAGTTCCTGCTGCCAAGACTGGTGATCTGAGTTTGATCCTTAGGACCCACGTGGTAGAACTGACTCTCAAAAGCTGTCCTCTGTCCTCTACATGTGTGCTATGGCAGGCACATGACTGCACACACACACACACACACACACACACACACACACACACACACATTGAAACATACACACCTGAAACCACATTTTAAATTAAAAACAAAACCCAAAGAGTCAGGAGCCATCACAGGACAAGCTAATAGCTAGCAGGTGATCTTCCTGGAATGGTCTGCCTCGGAGTATAATAACCCATGAGAGATTTGCTCCGATTGGATTTGCATGGCCCAGGAGAAAATCACTTTAGGAAGTATTCCTGCTATTATGTTTTTCCTGTTATTATTAAATATATTTAACAATCCCTGGGTATTAGCCCATCCTTTTGAGCAGATCTCTGCAGATCTACAAAGATGTACTGCCTTATAGTGATGCTATATAGACAAATAGATGACTTAATTCTTTATGATGATCCTATAAGAATTACTAAAATTATTTACTAAGCTCTTTTATAGTGGGACTGCTATTAGATACTTTTCTGATAGTTAAAACTGCAATGAGAACTTTGTCAGTCTCCATTGTGTCACCAATGAATTGCTTCTAGATAGTAACCAGACTTTCTACTACTCAAAGCACATTCCAAAAGGTTGTAAAACCATTAACCAAAGGTCATAAAAAGGGAACTAATGATTTATTATAGATGCTAGGACAGAAGATAAAATATTGACTGGATTTATCTATACAAAACATCACTAATAACTTAGTTATGTTTTTTTAACTCTCAAAGAACTTGTGGAACTTTAACAGGTGATGAATCTTTAGTGGGATAATTACTCCTAATGGATATGCATGTAAACACTCTCTGTTGTAAACTTCAATTTATGATTTGAATTTGTGTAAACTTATGATGAACTTTTTACCATCTGATCATATATTCTGAAAGATATATTAGTGCTAAGGACAAAAAAGAAAACGAAGAACTGGGCTGGGGAGAACTTTTTAGACAGAACACTTTTTAGACAGATCTGAACAGAACTTTTTAGAATAGAACTGAGGATAATTCTTTTAGTCAGGACACAAGAGAACTTTTTAGACAGAATTGAACTCAAGAAGAACTTAGAAGTAAAGGCATATCATTGGGAGAAAAGGCATTGAGAGTAAGAGCATTGCTGTGACATCAGAGCAGGAAGAGAGAGCAAAATGAGGAGAATGCAGAGTGGAATAACTTAAAAACTATAGGTAACATAAAAATCTAAGAGAGCAATGTGCAGAATGCAGAGGGAAAGAGGGGAAAAAAGAAGCTGCATAGAGCAGAAGGAGCAGGCAGGCTTCTCCTTACCATGGAACAGAACAGGTCTTTTCTGAATAGCAAGGCAGGCTTATTCTTATAAAGGAACAAAGCTTTCTTCTTACAAACTTGGGTTTAACTCATTTAGCATTAAAAGGCTAGAAGCGTTTTCTTTCCCTATGCAATAAAGATTGGAGCTCTTTTTCCATGTAGAATCAGTGAGTTCTTTCTGAAACCTGTGCTTGGTCTTTTGCTCCAAATGCATATGTAAGTATAGACATGTGTGTGTAAGTATGTAATTGTGAGAATGTATGCAAGTTGGACACAACTGGTTTTAATGGAAGTGTATAAATGCACATATGTGATTCTGTGTATGAATTTACGTAAGTTTATGCATATTTGCTTATATAAAAGTTTTTCCTTCTGCAAGCATTATTCTCTTCTGGTTCAATAGAAGTTTATTGTTCAATAGCCTGACAAGCAAGAGGCATCTGAACAAAAGGGAAAAGGTTCTAGCAATTAATCCTTTACTTAATCTCCTGCTGTTTTAGGATGAGGTGCTAAGTGCCAGAGACTGCAGTTTCTGGCCTCAGGAACATAGAAGGCAGGTCAGCTACAGTAGCACAGTAACTTGGACTTACATAAGTGAACTTTTAATTATTATACTACAACCCTAAATATCTCACAAGACAAAAGTCGTACCCTCCGCCAGTGATCTTCCCCCCTCTGCTACCTCAAGGAGAACCAACAAGAAAGAAGACCCCACCGGGGCTGGCCCCCAGCACCAAAGTGTTCATGTTCAGAGTATTTATCTATCCAGCCCACCTGTAGTGCTTTCTGGCTTTCTGATGGTGCTATGGATTTCCAGCATCAAGATCTAATGTTTCTCATTGCACTCTGGGCAGTAGCCAGGTACCCAAAACAGCAGGTCTCGTGTATAATTTTTATATCTATTTCTCCAACAAAGAATTTTCTCAAAGGCCATCTTCCCTGATTACTGTTCATTTCTTCCTATAGCTTCTACCTGGAAAAAAAAATGTGTCTGTTTGGAGCTCTGTGACCAAGAAAACAGTTGAAAATGGAGGCCATGTATGGAGATTACAAGCAGCACAGTGGAGTGGGACAAGGAGTTGTTTCTTTTAATAAATCATTAATGTAAAAGTCTCAACACTTCTTGAGTTATTTTCTTGGAGGTTGTTATGGTTTGAATATGACACGTCTCCCTGGTCCATAGTTGATTGTACTGTTTGGGAAGAAGGGGCCATGCTGGAGGAGATAGGTCAACGGGGTATTTTGTCCTGGGCTTGACATGTTTCTTGTTGTTTCCTGGTTGCTTTAGGTGAGAAGCCTCAACCAAATACCCCTGCTGTCACGATCATTCTCCTTTACTACAAAGCAGCAGATTCAGTCAAGCATAGCCTTAAACCACCTAAAATAGTGCACCAAAAAATTAATAATAATAATAATAATAATAATAATAAATCCTCCTTCCTTTAAACTGTTCCTCTCAGTTATCTAATCCTAATCGTGAAAAAGCTACTAATGTCAATACGTTAAGTTTTTGTCTTAGTCAGGGTTTCTATTCCTGCACAAACATCATGACCAAGAAGCAAGTCAGGGAGGAAAGGATTTATTCAGGTTACAATTTCCACATTGCTGTTGATCGCCAAAGGATGTCAGGACAGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGACATGGAGGGATGTTACTTACTGGCTTGCTTCTTCTGGCTTGCTCAGCCTGCTCTCTTATAGAACCAAGACTACCAGCCCAGAGATGGTCCCACCCACAAGGGGCCCTTCTCCCTTGATCACTAATTGAGAAAATGCTCCACAGCTGGATCTCATGGAGGCACTTACTTCCCCAACTGAAGCTCCTTTCTCTGTGATAACTCCAGCTTGTGTCCTATCAGAACATCAAGAGTAGAAGGAAGCCATATAGCTCACTTTTCTGTCACTGCCATGTAGAAGAGTGCTCTCCAACTCCAGCCAGAATCTGAAACTCTAGCAACGTCAGCCCTGCCCAAGAGCAAACTTTCTGAGAGGGTAAGGGGTGGTGGGTGGCCTTATTTCTGCTGCCTTTTACCTATCCTAATCACAGAACGACTTGCAACCTACAGTTCAAATGATGGCTTTCCGGAACTGGAGCACACACCCAGGCCCTGCCCCAAGCGGTGCCCCGGAAGTATTTCCCAGGCTTTCTCACGGAGGAAAAATGGCGGCGGTGCAGGTCGCCGCCTCTCTTCCTTGTGGGCAGCCGCGAGAGGCCCCGCGGGAACTGTCGCCGGAGCAGGACGATGGGTTCCGCCGCTTGTCTGCCAGGCTCCGCGCGCTGCAGCCGGATGACAGCACCGTGTCCCGCATGGAGATTCACCTGCTCTTCGACCAGCTCATCTCCGAGAACTACAGCGAGGGCGGCGGCGTGGCCCCCGAGGTAGGCGGCGCGCCTCATCCTGCCCGGACAACGGCTGGTTCCTCCCTCCGGCCCCTCCGGCTCTCACCCACGTCCCGGATGACTTAGTTCCAGGCTGGCTTTCTCCCTCGGTCCGTGCCATATCTCTCTGCCCTCCATCTGAAAGCTTGCAAGCCAGCATCTTTTCAAAGGCTTCTGCTCTGACGCCCTCAGCTGCCATCCCTGGTGAGCGACTCCGGGAATGTCGGAGCGAGGGTAGTCTTAGTTGATTTCGCTGATGTACGCACTGAAGCTCACAAGAGATCCAGCGTCACGGTTTGGGCAAATGGCAGAGCTAGATTGGAACCTAAATTTGTCGTAAGGCCTGTATTTTCTGTTTTGTTTCGGCACTAGCTCTCGTTTGCTTCCACTTCTCCTGTCAGGATCATCTCATTCATACACATCCGCTCTTGTATATCACTGATTCCCAGAACCCCTGAGCCAGTCCTACCTGCGAGCATCTCAAACTTGATAGCTCGTATAGATGAATACATTGTCTCCCATACTCCTAATCCCCTATTCATTCTACCCTACCCCACCCCAAATACACACAAGGCATGCACACTGCTTCTTTTCTTTACCCTGGAGCCTGTTGGATTAAAATTCTTCAGTCTGTCTCAAACATGTTTCTCAGTTCACACTCTTCTCTCCCTAGTCTCTCACTGATCTTCCTCTATTCTAGCCCCATTCCAGACTATTCTTTGCCAGAGTCTGCTATTGGAAAAACAAAAACAAAAAACAAACAAAACGCATACAAGTGTACGCATAATCAGAGATTTTCTATGTATATTGGCAAATGGCAGAAGCAAGCGGCCAAACAGTAAGAGAAAAATATGGGGCTTAAGGATGTAAAGGGGAAAAGAATCCCGAGCAGCTCACCACGGATAAGGGAGCAGAGTGGAACTAAGTGAAATTAAGGGAGCTTTTTATTTATATACTTGGCTATTTTGCACAACAACAACAACAAAATGTGTGCCTTTCCATTCTTCTCTCCTTGTTCTCCTCTTGGGTTCAGTCACTTGGCACTTGGTGCCTCGGGTTCAGGCACCTGAGGACATTGCGCATCTCTGAGAGTTCCCCCACTCCATCCCCTCCCCTTGTTCTTTGATTACTTGTCTGTCTGGCAAAGTTCTATTTGTTCTTCAGATCTTCTTTGTTATCTTTCTGTCTCCCTCTCGAGGAGACAAGTTCTCTGTGGTTCAGGGAAGTGTTTGGTTCATCTTTGGAGTCAAAAAAAAAAAAAAAAAAAATACCTAGCCTACTGCCTGACACGTACTATATGCCCAGAGCCTTTCTGTTCATTAAGCATCTGTAGATGGTGTAATCTGTGGATAGCATCCTCTCCTCTCTCTCTCTCTCTCTTTCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTGTAAGTGTTATTTCTGATTTGCCCAATAGCATTGCATGCTTGGCTCATTTGGTATTTTTAGCTGTAGCTGTAGCATGTGCGTTTCTGTGTTGGCATTTATTTAATAGCACTGTATGGTAAAAAGCGCTTTTCCTTTCCTTTAGTCCTAACAAATTTTTGTTAACTTCATTCCTCCAGCCCCCACTTTCTGCCTCATCTTTGACTCGGATATTTTTTTTTTTCTCATTGAGTGTGGAACAGTCAGTGAGCTTTTCCGTTTGACTATGGGATTTGGGGATGTTCACTTGGCTAACAGGTGCATTTATCTTTGTTTTCCTTTTTTTTTTTAATAGGATGTCAGTGCTCTTCTTGTGCGAGCTTGTCAGCTGGTGCCTCTTAACCAGAATCACCTTGTGAGCAAAGTGTGTCAACTGATCCATCGTTTGCTTAACAGATTACAGGTAATCCTGTATGTAACCTGAAGGCTCAGATCAGGGAATACCACACCCGTTGTAGATCTGATAGGCTCACCTTTTGGTTTTGTTCTGAGACGGGATCTGAGCTCCGGTTGGCCCGAGAACTTTCTGATCTTTGTGTTCACTTTTTAAAGTGTTGGAATTGCAGATGTTTGTTGCTACACCCAAGTCAGTTGTCTAATCTTTTGAAAAGCCTGGAAAAGCAAAATGTCTTGGGCTGGGAATGTAACTCAGTGAATTACATTCACTTAGAGCCTTAGCCCCCTGGCAGACATGAGGCCATGGGTTCAATCCCAGCATCAGTACCACCAAAACAAAACCAAAACAAAACCAGTGCTTAGTAAGAGTACATCATTATATCCAAATGCAACTGACCAAATATATATTCTGATTTGAGAAATTGTAGACTTTTGCTTATGAGAAATATTCTTTTATGTTTGGTTCAATAAGCTAACATCACTCATGCCATCTTAGGTTTTTGGGGTCTTGTTTATTTGTTTTGTTTTCTCATGAAAAATAGAATTGACCAAGATATATCCCAGAACAAAATTACATGATTCCCCATTTCTTTCCTACCAATATTTTTTCCTGAAATTATTATTTTTGCAATCTACATAAAACCCCCAAAGTATCTTGGGATCAGAGAGCCGCAGGATAAAAAGTCCATAGTTATTTCTGTGAAGGTCTCTTCTGCAGTTCTCCGTCTGTAAAAGTTCTGAGTGAGTCCCTGAGTAGATGACTATATGGATATCAGCATTGTGTCAGCCCGAATCCTTTGGACTGCAAGAGAAGAGGGCATCCAATAGAACCAGAACCCTGTGGACAGGGAGTAAGATGCGTGCTGAGGTGTAACCAGCTGCATGTCTTACAGGAGCTGAGGAGTTTCCAGAATGTCACAGGTGGCTGTGGGGGGTGGGGGGTGGGGTGGCTTCTGAGTTCTTACTGTTGTCCAGTGTGCTTCATCTTTGTGACACTGGCAGCTGGAAAACTTCCCATCACCTGTGGCACCTGCCGCCCATAGCCAACTTCTAGCATTCGATGATACTATATATGAACTTAAGTGTCTGTAATTTGTTTTTTTGGTTAGCCTACTCTCTCTCTCAAGAGGATTATAAACTCTGTGGGGGCAGGAATGTGTCATATCTTCTTGGTATGTTCCTCACATCATGACAGATAAAGAGTAGGTTATTTAGCAGACATGTTTATTTGCTGTGTATTAGTTGAGTAACTACTATGTATTAATCAAATTTAATTGAACTAAGACATTTCAGTGTATTGACTCTTATATCTGCATTTATATCTGTCAGTTTCTGATTGGTTCCAGGAAAAGTTACAAGGACTACCAGATTCTACTTTGGAGAGATCACAGGGGCAGAATGAAGGAGACAGTGGGGCAAGGAGGTCTCCCCTATCCTGATCAGACTTGCCCATGCGTTCTCTTAAGTGAGAGCATCTCTGTTTTCTCCTTTCTCGGCAGGTGGTTGTGGATGAGCCAAACTTAGATTTCCTGTTGACCTACACGATCTCCGCTCTTCAACAGTGTAGCTCTTGGACACACATGGAAATTCTTCAAGCCCTGGCAGCTCTGGTTTACTGCAATGGCTCAAAATGTCAAAAGGTAGTGTCTTAGGTTCTGTTCTGTGATATACATAATGAACAAAAGCAACTTGGGGAAGAAAGGGTTTATTTCATTTTACAGCTTATAGCCCCATCATTAAGAGAAGCCAGGGCAGGAACTATAGCAGAAAGCATGGAGAAATGCTGCTTACTAGCTTGCTCTCTTTAGCCTACTCAGCTGCTTTTTTATACAACACAGGCCCAACTGCCTAGTCCTAGGGATGCCACCACTCACAGTGGGCTGGTACCTTCTACATTAGTTAGCAATTAAGAACATGCCCCACAGACATTCCTGCAGGCCAATCTGATGGAGAAAATTCCCCAGTTGAGGTTTCCTCTTCCCAGGTGACTATAGGTTTGTGTCAGGTTAACTACTGAAGCTAACTATGACAGGTAGTTCAAACTATTATGTCTTTCCTTTGAAAGGCTAGTAGCACAGACTTGAGAGCCAAGATGCCTATTTTCCAGTTTCCATCCTGTCTCTAATTGCGTGTATAGCCTAGGGAATTTCTTACTGAATGCCCTGGAGCCTTGTTTATTCAGGTATGAAATAGATGCTCCAAGGATGAAGTGAGATAGTATTTGGCACTCTATTAACTGTCAACCATTATTATCATCACCCTTTGCCCATGTAGCCAAGACTAAAGAACCCCAGCAATGACCTAGTAATGATTCTTACCACTGATTTCTGGCATACGTTTTGGGTAGCCATTGAAGGATTTACCTAATAGTTCATTTTTTGATAGCTAACTGCCTTTTAGTTATGAAACTATTGTCTAAACAAGTACATTCTTCCTAGAAGCTGTTCTCAGACCCACTCTGTTGATAATTTGTTTCAGCATCTCCCAGACTTGCTGGGCAAGAGTGGCCTCTTGATGAAATTGAGTGATTTATCTCACTCTGACCCTGAAGTCAGGAGAGCTGCAGTACACTGTATGGCAAACTTATGTCTCAGGTATGTGGATCCAGGTTTCCCAGTAATAGAAGGTAAGACCTCTCCTGGGCCCTCTGTAGGTTGTTCTGTCTGCCTGAAGCCAACTTAGAAAAGTGACATAGCCTAAGCTATGCCTGGTGGCACATTCCTGTAAACCTAGCACTAAAGAGCCGAGACAAGAGGATGCTGTTCCGCATAGCAAGTCCCAGACCAGCTTGGGCTCCAAGCTTTTATTTTGACAGAAAGAAAAGGACCCTAATTTACATTCCCCATGCCATTCAACAAGGTTATCTTATTGTTTGTTTTTAAAAACTATCAAGAAGTGAAGGGAGAAATGCATTCTGGAAGTGTAAAGGCGTGTAATATGTCAGTTTTATTTTCCGCCAATGTAAATTTTAACCATATTCTTTTAGAAAGAATATTCATGTGAGTTTAGGCTCTTGTGAGTTTCTGTTAAGTTCAGTTTCTTCTTTAAGAAGATGGAGCAGGTCTGTAGTATGCAATAGCGAACAGGCAGCACACACTTGACCTTGTCTCTGGGTAGCACTGCACCGTCCAGTGCCTAGGAAGGCAGCCTCAGTCTTTCTCCAAAAGATCTGTCTGCACTATTTCCCTCCTGTGACTGAAGGGCAAAACCAAAACAGATCTTTTCTTCCCCACATTTCCTGAACTACACTTCTGTGACGTTTTCCTATAGAAATTTCTCTTCCTTCTTCATGTGTGTTCAAGCCTGTGCAGGACAGCAGTAACTAGTCATGCAGGACAGCAGTAACTAGTCATCCAAGAGTGCCGTTTCTGGAGTACAGCAGTGTTCAGGGCGTGTTCCAGACTTAGCGGTCTGCATTGCCGTTGCTAATGCTCGCTCTTCTGGAAGCACCACTTCAAAGGCGACTGAATTGTCTCCTCTACATGTGTTTCAGTGTGCCAGGACAGCCATACTTGGAGGAGCCCTACCAGCATGTCTGCTTCCAAGCTTTCTTGACCATTTTACAGTCTCCAAAATCGTCTGATATGGATGATATAACATTTTGCATGGTAGGTGGGGCTGCTACTTGGCATCAGAGTATTGCAGGCATAGGACAGAAGCTTCAGGGTGTGTGTGTGTGTGTGTTTGTGTGTGTGTGTGTGTGTGTAAATGGTGTCTCTATTCAGTGGCAGAAGTCAGATCTGTGTGTGTGTGAATGGTGTCTATATGTCAGCAGCACTGAAGTCACACCTTTGGACTCAAATGATAAGGATCAGTTTTACTTGTTTTGGTTTGCTCTCAGTTTTGTTTTGACAACTTAAAAGCCCATATTCTTTCTCCTTCCCTGTCTAAAAACAATACAATGATTATGACTGATTTTATTAAAAGGGAGGAAATCTGCTCAACAATATCCTTTCTAATGACAACTACGATAATGTGTGGGCATATTTTAAATTAAGCATACATTTCAAACACTGTAGATCCAAGATAATAAAAGTTATTTTGGGCACCTGGCCCATACCCAAGCCCAGGAAGCTTCATTCCTTGGGCTGAATGAGCAGCAGGTTTACCATTTCGTGGGCTGTTTTATAGCCCTCTGCTTTTTGAAGGTTTAATGCCTCTTGGACAGCTTTCCAGTGCACATTTATATATGCGTCATGGTTTCTAAGGGCAACAAATGGAATAATTTGGTCAGATTATGCCCTGTTGCCTCCATGTTCTACCCTAAAATTGCACTGATAAAACCATATTGCGGGGCTCTTATGCGGTGCACAGATACAGCATTTAACGTGTGTGACCTAGTCATGTCCTTGTTGTTATCTTTCAGTTAGCAAGCATTAGCTTCTGGTATCATCATTGTAACTATAATCCTGAAAATTAACTTTCCCCACTATTTTAACAAGTCTCCAGGTCACACCCTGCCCTGAACAGATTATTACTGAAGGACAGAGCCCTGGAGATGAGGTTACTGAGCTGAGAAATCTGTGGTGATCGGAGCTGCCCTTCCACCCTCCCATGCCTCGCCTTCCCGAGCCACTCAGGGTGCTTTCTTACAGAGATCTTCCCTTCACCTCTCTGGTCCTTTAAAAGTTTTTAATGGCACTTTAATATGTGTATGTGATCATGTGGGCATGCCATAGCACTTGTTTTGAGGTCAGAGGACGTTGGAGAAGTTGTTTGGTTCTGTCTTTCCACCATGTGGGTCCTGGGAACTGAAATTAGGTCTCCAGGCTTGGAAGCAGGTGACTGTCCCTGCTAAGCCGTCCCACGGGCCCTCCGCACCCTTCCCATGCTCTCCGCTGCTCCCTTTCTGGCTCATCATTTATACCTGGTTTATGTGTGTGTACAGTAAGACTACTCAGTAGAGAACTCTCCTGGAAAGTGGCCCAGAGTGCTCATGGGTCTGTTCTCAGGGTCCTCATCTCTGCCGAGTGCTCCAAAAAAGTTTTCACCATCTAGGGGACTTTTTCCTCTTCCATTCTAAGTGGTCCCAGAGGAGCCAGATTTTATCCTTCCTTCTAACTTGCATCTCTGCCTCCCCACCCTCAGCTGTTACAGAATGCACTGAAAGGCATACAGTCGCTTCTCAATGGTGGGAAGATGAGGCTGACGCAGACCGAGCACCTGGGAGCGCTTCTCGCTGTGCTGAAGGTGAGGCAGGGGTCCGAGGGTCCTCACTTCCTGTTTCACACCTCAAGTGTGGGGCTGCCCATATCCTCTCCTCTCTTCAGCTAACTGTGGGGTATAAGAATTTCTCACATGGGTCAGGCAGTGGTGGCGCACACCTTTAATTCCAGCACTTGGTGGGCAGAGGCAGGGGGATTTCTGAGTTTGAGGCCTGCCTGGTCTACAGAGTGAGTTCCAGAACAGCCAGGGCTACACAGAGAAACCCTATCTCAAAAAATCAATTTAAAAAAAAAAAAAAAAGATTTCCTCACATGGATTTTAAGAGCAGAATAAAATATTCATGTTCAAGCATTCTCAGATAAAAGAGATTAAGAGCAGAAGACAACCACTATCAAGCAAATATATCAAAGTTTTAAGAAGTACTTTTGAATCTTACAAATCTATGATGAGATACGAGCTGTAGATAATTTAATTACAGATTATCGTGAACCCACCTTCATCTGTGTGTTCTGAAGGAGCTGTCATGATTAGCCTCTGAGTCTACACTCAGTAGGAAAGTCAGGCCACCCTGAGAGGCCCAGCACCCTTCAATTCATCCAGGGAGTCAGAGCCTCAGTGTTCACCTCAGGTCAAAGACGTTTGTTACAGTCTAGCTTCGTGACCAATATCTTAGTTATTTTCTCTTAACCCCTGAATAAGGACAGGCAGATATTACAATGGTTTGAATATGCTTAGCCCAGGGAATGGCACAATCATGAGATATGGCCTTGTTGGAGTAGCTATGGCCTTGTAGGAAGTGTATCACTGTGGGGATGGGTTTTGAGACGCACCTCCTAGCTGCCTGGGACACAGTCTTCTTCTGGCTGCCTTTGGATCAAGATGTAGAACTCATGGTTCCTCCAGCATCAAGTCTGCCTACAGGATGCTGTGCTTCCCCACCATGATGATAATGGACTGAACCTGTAAACCAACCCCAACTAAATATTGTCCTTTATAAGAGTTGCCTTGGTCATGGTGCCTCTTCACAGCAATAGAAACCATAATTAAGATAGAAGTTGGTACCAAGGACTGGGGTATTGCTGTGATAGGCTTGACCATGTTTTTGTTTGGAGGAATGTGCATTTGGGGACTTTGGATTTGGAAAGCAGTGGGATGCTTTAAGTGGGGCTCAGTGGGCTAGTTCAGTAGGAATCTGGAAGACATCGATGCTGAGAATGATTTGAGTTGTGGGGCCTGGCTCCAGATGTTTCAGAGGAGGATTTTAGTATGTGGCCTAGAGACTCTTGTGATATTTTGGTGAAGAATGTGGTTGCTTTTTGCCTTTGTCTGAAAAGTGTGCCTGAGGCTAAGGTGAAAAGATTCAGATTGATTACATTGACAAAGGACATCTCAAATCAATCAAGTTTAGACTCTGTCCTGCGGTTCACTTTTGTAAGGAGCATTTTGATCAAGCGTAGCAAGCTTAGAAAGGAAAAATACAAAATTTATGGTTCAAGTATTAAATGGGCAGCAGGAAGTGCAACGGAGCTAAGTCCTGTGTTCAAGGATATTAAATGGACTTTAGGGAGTGGTGACCTCAGGACAAGGTCCCTCCCAGCTGAGCTTATTGTTTATGTGTTTGCAGTTGATTAAGGGATAGTTATATGATCCAGATCTTGGGGCTTTTGTTCTGAATCTTGAGGCATAGTGGCCATGAAAAGTTTAGGTCCAAGCCTGGTGGAGCGCCTTTAATCTCAGCACTTAGGAGACAGGCATGCAGATCTCTGAGTTCAAGTTCTGTCTACAGAGCAAGTTCCAAGACAGCCAAGCTTAGACAGTGACAGAGTACACAAAGCTGGTGATGATGTATAGAACAAGGGGCCATGTTCCAGCCCCGGAAAGCAGCAGAACTCAGCAGCTCTCACCATGTGGCTCTGGCTTTAGAGTCAAGAATAGAAGGGACTAATGGGACAATCGATGCTGGTTAACTAGAGCTAAGAAATTAGTGGTGATTAAGGAGAGGCCAGTATCACTGAGATGAAATCTTCTGGGAAGTGCTTTCTGAGAGCACAAAGAAGCCATGTTCCAGAAATAGCTAAGGTTGCACCTCGTGCTGCAGCTGGACTTGGTAACATGTAAGAGTCACCCAGATGGTACTGTTTTGAAGTCATGAGGGGATCATGGAGAGAAGCTGAGACTCGGCACTGTGAGAGTCCAGGAAAGGCCATTGGTGAAGATGCAGCCTCTGTTGCAGTGGACAGCTCAGGACTAAAGGGATCATGCAAAGAAGCTGAGGCTTGGCACCATGAAGAGAGCCTATGAGAGGCTATTGGTGAAGCCTAGTTGCAGGGAAGACCCCAGTGTATTAGAGATGCCAGTACCATGGGATGAGTACCAAGAACAGCAGCAATGGAGTGGAGTCAACTAGATCCTGGAGTGCTACAGAAGGCACAGCTGGAGAAGTGACCCAAGTCCTTTGGAGGAGCCCAGAAGATCATGTGTGGATCCCAGACATTAAAACAAGAAACTATGAAGTTCAAGTTACCTTAGAGGCGTCAAGATATTAAAGATGCCAGAGCCATGGGATACCTGCCGAGGAAAACTGCTAACAGTGGTACAAGCTCAGGAGAAAGAAGCTGTTGCAGTCAACAAAGATGAAGGGAGCTGTAGATCTGAAAAGCTCATTAATATCAGATATAGAGATGCAATGTTTGGAATTTGCCCAGCTGGTTTCCTGTCTTGCTCTGGTCCAGTATTTTCTCAATGTGACGTTTTGAATGGTAGTGTATATCCTCTGATGTTGAAGTATGTGATCTGCCTCTTGATTCTGGTTTTATAGGAGATTACAGTTAAGAGATTACATAAATCTCAGAGGAGCCTTTGAACTTTGGACTGTTAACATTGTTGAGATTGTGATAGACTATGGGGAGTTTTGAAGTTGCATTATACTATAGCTAGGTATAGCTTCCATAGACTCATGTGTTTGAAAGGGAGTGGCACTATTAGGAGGTGTGACCTTGTAGGAAGTGTGTCACTGTGGGGGGTGAGCTTTAAGACCCCCCCCCCCAGCTGCCTGGGAGACAGTTCTGGATACCTTCAGATGAAGAAGTAGAACTCATGGCTCCTCCAGTACCACCTCTACCTGCCCGATGCCATGCGATGCCATGACGATAATGAATTGAACCTCTGAAAACCAGTCCCAATTAAATGTTTGCTTTATAAGAGTTGTCTTGGTCATGGTGTCTCTTCACAGCAATGCAACCCCAACTAAGACGGATATGGAAATACATAAAAATACATACAAAATATATAAAAATACATAAAAATGATATATTAATGGCTAGAGAAATGGCTCAGTGGTTAAATGTGCTTGTTCCTTTCACAAAGGACCCAACACATACCTGGTGACTCACGCCGTTCACAACTCCAATTCCAGGGTATCCAACCCCTTCTTCTCACCACTGCAGTCTCTGTGTACCCACATGGTCTACATACATGCAGATGGAATACTCATGCACATAAATCTAAAGAGGAATAAAGGATTGGGATTTAGAAGAAGAATAAATTGGCTTAAACAGTTTTTGATTAATAAAAAATAACTGGATAAAAGTGATTTGACAAACAAACAATCATAGATGCTCTAGTCTAATCTGGTGAGGCCAGTGCAGACCCAGCTGCAGCTAGATAAAACTAAGCTCTTCCCCATCCCAGTACTTGCCACGCCCAACCCTGCTTGGCTTCTGAAATT
GF3 -1 -1 1 CAGGTCAAGATCCAGCTCCGGCGTTCAAC
GF4 -1 -1 1 CAAGATCCAGCTCCGGCGTTCAAC
EX52 -1 -1 1 CATGAAGAAGCACCTACCAGAGGATGG
EX32 -1 -1 1 CACAGTCCCAGTCACCACGGCAGAG
GR3 -1 -1 1 GAATCAGTGATATACAAGAGCGGATGTG
GR4 -1 -1 1 CACAAGGAAGAGAGGCGGCGACCTGCACCG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000051748 -- 83562735 -- 83562835 ENSMUSE00000420571 -- ENSMUSE00000420571
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 11, start 83562735: end 83562835
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:11:83556235:83557234_148 G3:11:83566335:83567334_134
ACCEPT (0) gap pair G5:11:83556235:83557234_148 G3:11:83566335:83567334_147
ACCEPT (0) gap pair G5:11:83556235:83557234_148 G3:11:83566335:83567334_142
ACCEPT (0) gap pair G5:11:83556235:83557234_537 G3:11:83566335:83567334_134
ACCEPT (0) gap pair G5:11:83556235:83557234_537 G3:11:83566335:83567334_147
ACCEPT (0) gap pair G5:11:83556235:83557234_537 G3:11:83566335:83567334_142
ACCEPT (0) gap pair G5:11:83556235:83557234_532 G3:11:83566335:83567334_134
ACCEPT (0) gap pair G5:11:83556235:83557234_532 G3:11:83566335:83567334_147
ACCEPT (0) gap pair G5:11:83556235:83557234_532 G3:11:83566335:83567334_142
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

webmaster@sanger.ac.uk

Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK  Tel:+44 (0)1223 834244